TOP3_YEAST - dbPTM
TOP3_YEAST - PTM Information in dbPTM
Basic Information of Protein
UniProt ID TOP3_YEAST
UniProt AC P13099
Protein Name DNA topoisomerase 3
Gene Name TOP3
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length 656
Subcellular Localization
Protein Description Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Essential for proper chromosome segregation in both meiosis and mitosis. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA. The TOP3-SGS1 protein complex may function as a eukaryotic reverse gyrase introducing positive supercoils into extrachromosomal ribosomal DNA rings..
Protein Sequence MKVLCVAEKNSIAKAVSQILGGGRSTSRDSGYMYVKNYDFMFSGFPFARNGANCEVTMTSVAGHLTGIDFSHDSHGWGKCAIQELFDAPLNEIMNNNQKKIASNIKREARNADYLMIWTDCDREGEYIGWEIWQEAKRGNRLIQNDQVYRAVFSHLERQHILNAARNPSRLDMKSVHAVGTRIEIDLRAGVTFTRLLTETLRNKLRNQATMTKDGAKHRGGNKNDSQVVSYGTCQFPTLGFVVDRFERIRNFVPEEFWYIQLVVENKDNGGTTTFQWDRGHLFDRLSVLTFYETCIETAGNVAQVVDLKSKPTTKYRPLPLTTVELQKNCARYLRLNAKQSLDAAEKLYQKGFISYPRTETDTFPHAMDLKSLVEKQAQLDQLAAGGRTAWASYAASLLQPENTSNNNKFKFPRSGSHDDKAHPPIHPIVSLGPEANVSPVERRVYEYVARHFLACCSEDAKGQSMTLVLDWAVERFSASGLVVLERNFLDVYPWARWETTKQLPRLEMNALVDIAKAEMKAGTTAPPKPMTESELILLMDTNGIGTDATIAEHIDKIQVRNYVRSEKVGKETYLQPTTLGVSLVHGFEAIGLEDSFAKPFQRREMEQDLKKICEGHASKTDVVKDIVEKYRKYWHKTNACKNTLLQVYDRVKASM
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure
ASA (%) Reference Orthologous
Protein Cluster
30PhosphorylationGRSTSRDSGYMYVKN
CCCCCCCCCEEEEEC
30.6128889911

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources

Oops, there are no upstream regulatory protein records of TOP3_YEAST !!

Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference

Oops, there are no descriptions of PTM sites of TOP3_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10)
Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of TOP3_YEAST !!

Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions
SGS1_YEASTSGS1physical
11124263
SGS1_YEASTSGS1physical
12036100
SGS1_YEASTSGS1physical
15899853
RMI1_YEASTRMI1physical
15899853
RAD16_YEASTRAD16genetic
11459632
MRE11_YEASTMRE11genetic
12399378
RAD1_YEASTRAD1genetic
12399378
RAD50_YEASTRAD50genetic
12399378
RDH54_YEASTRDH54genetic
12399378
SGS1_YEASTSGS1genetic
10757756
XRS2_YEASTXRS2genetic
12399378
SRS2_YEASTSRS2genetic
11156978
MMS4_YEASTMMS4genetic
11139495
SLX1_YEASTSLX1genetic
11139495
MUS81_YEASTMUS81genetic
11139495
SLX4_YEASTSLX4genetic
11139495
SLX5_YEASTSLX5genetic
11139495
SLX8_YEASTSLX8genetic
11139495
SRS2_YEASTSRS2genetic
11139495
RAD51_YEASTRAD51genetic
12399378
RAD54_YEASTRAD54genetic
12399378
RAD55_YEASTRAD55genetic
12399378
RAD57_YEASTRAD57genetic
12399378
SGS1_YEASTSGS1genetic
12509234
SGS1_YEASTSGS1genetic
12399378
SGS1_YEASTSGS1genetic
12036100
SGS1_YEASTSGS1genetic
8913739
SGS1_YEASTSGS1genetic
7969174
ATE1_YEASTATE1physical
16488395
KPC1_YEASTPKC1physical
16488395
SGS1_YEASTSGS1genetic
15616582
PIF1_YEASTPIF1genetic
16816432
SGS1_YEASTSGS1genetic
16816432
RAD54_YEASTRAD54genetic
16816432
RAD55_YEASTRAD55genetic
16816432
COM1_YEASTSAE2genetic
17314980
MUS81_YEASTMUS81genetic
17314980
MRC1_YEASTMRC1genetic
17314980
NUP84_YEASTNUP84genetic
17314980
PRI1_YEASTPRI1genetic
17314980
NU133_YEASTNUP133genetic
17314980
MMS4_YEASTMMS4genetic
17314980
SLX4_YEASTSLX4genetic
17314980
SLX8_YEASTSLX8genetic
17314980
DPOD3_YEASTPOL32genetic
17314980
UBC4_YEASTUBC4genetic
17314980
SUS1_YEASTSUS1genetic
17314980
CUL8_YEASTRTT101genetic
17314980
PCNA_YEASTPOL30genetic
17314980
UBP3_YEASTUBP3genetic
17314980
SLX1_YEASTSLX1genetic
17314980
RT107_YEASTRTT107genetic
17314980
XRS2_YEASTXRS2genetic
17314980
THP2_YEASTTHP2genetic
17314980
ASF1_YEASTASF1genetic
17314980
SRS2_YEASTSRS2genetic
17314980
RAD55_YEASTRAD55genetic
17314980
SGS1_YEASTSGS1genetic
17571075
RMI1_YEASTRMI1physical
17693398
SGS1_YEASTSGS1physical
17693398
SHU1_YEASTSHU1genetic
17671161
RAD51_YEASTRAD51genetic
17671161
RAD54_YEASTRAD54genetic
17671161
SGS1_YEASTSGS1genetic
18272435
SGS1_YEASTSGS1physical
19158389
SGS1_YEASTSGS1genetic
19214189
SGS1_YEASTSGS1genetic
11585898
SHU1_YEASTSHU1genetic
15654096
SHU2_YEASTSHU2genetic
15654096
CSM2_YEASTCSM2genetic
15654096
PSY3_YEASTPSY3genetic
15654096
RAD52_YEASTRAD52genetic
20541983
SRS2_YEASTSRS2genetic
21459050
RNH2B_YEASTRNH202genetic
21741981
RNH2C_YEASTRNH203genetic
24550002
RAD9_YEASTRAD9genetic
24278365
SGS1_YEASTSGS1genetic
24278365
SGS1_YEASTSGS1genetic
27811238
SGS1_YEASTSGS1genetic
25699707

Drug and Disease Associations
Kegg Drug
DrugBank
There are no disease associations of PTM sites.
Regulatory Network of TOP3_YEAST

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Related Literatures of Post-Translational Modification

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