PIF1_YEAST - dbPTM
PIF1_YEAST - PTM Information in dbPTM
Basic Information of Protein
UniProt ID PIF1_YEAST
UniProt AC P07271
Protein Name ATP-dependent DNA helicase PIF1 {ECO:0000255|HAMAP-Rule:MF_03176}
Gene Name PIF1 {ECO:0000255|HAMAP-Rule:MF_03176}
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length 859
Subcellular Localization Isoform Nuclear: Nucleus, nucleolus . Mainly concentrated in the nucleolus, and occasionally redistributes to single nuclear foci outside the nucleolus, probably sites of DNA repair.
Isoform Mitochondrial: Mitochondrion inner membrane
Peripheral m
Protein Description DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Appears to move along DNA in single nucleotide or base pair steps, powered by hydrolysis of 1 molecule of ATP. Processes at an unwinding rate of about 75 bp/s. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replication fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing..
Protein Sequence MPKWIRSTLNHIIPRRPFICSFNSFLLLKNVSHAKLSFSMSSRGFRSNNFIQAQLKHPSILSKEDLDLLSDSDDWEEPDCIQLETEKQEKKIITDIHKEDPVDKKPMRDKNVMNFINKDSPLSWNDMFKPSIIQPPQLISENSFDQSSQKKSRSTGFKNPLRPALKKESSFDELQNNSISQERSLEMINENEKKKMQFGEKIAVLTQRPSFTELQNDQDDSNLNPHNGVKVKIPICLSKEQESIIKLAENGHNIFYTGSAGTGKSILLREMIKVLKGIYGRENVAVTASTGLAACNIGGITIHSFAGIGLGKGDADKLYKKVRRSRKHLRRWENIGALVVDEISMLDAELLDKLDFIARKIRKNHQPFGGIQLIFCGDFFQLPPVSKDPNRPTKFAFESKAWKEGVKMTIMLQKVFRQRGDVKFIDMLNRMRLGNIDDETEREFKKLSRPLPDDEIIPAELYSTRMEVERANNSRLSKLPGQVHIFNAIDGGALEDEELKERLLQNFLAPKELHLKVGAQVMMVKNLDATLVNGSLGKVIEFMDPETYFCYEALTNDPSMPPEKLETWAENPSKLKAAMEREQSDGEESAVASRKSSVKEGFAKSDIGEPVSPLDSSVFDFMKRVKTDDEVVLENIKRKEQLMQTIHQNSAGKRRLPLVRFKASDMSTRMVLVEPEDWAIEDENEKPLVSRVQLPLMLAWSLSIHKSQGQTLPKVKVDLRRVFEKGQAYVALSRAVSREGLQVLNFDRTRIKAHQKVIDFYLTLSSAESAYKQLEADEQVKKRKLDYAPGPKYKAKSKSKSNSPAPISATTQSNNGIAAMLQRHSRKRFQLKKESNSNQVHSLVSDEPRGQDTEDHILE
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure		 ASA (%) Reference Orthologous
Protein Cluster
41PhosphorylationAKLSFSMSSRGFRSN
CEEEEECCCCCCCCC		18.7128889911
47PhosphorylationMSSRGFRSNNFIQAQ
CCCCCCCCCCCHHHH		33.5928889911
70PhosphorylationKEDLDLLSDSDDWEE
HHHHHCCCCCCCCCC		42.2221440633
72PhosphorylationDLDLLSDSDDWEEPD
HHHCCCCCCCCCCCC		33.8121440633
140PhosphorylationIQPPQLISENSFDQS
CCCCCHHCCCCCCHH		39.0421440633
143PhosphorylationPQLISENSFDQSSQK
CCHHCCCCCCHHHHH		26.5821551504
169PhosphorylationRPALKKESSFDELQN
HHHHHCCCCHHHHHH		45.2222369663
170PhosphorylationPALKKESSFDELQNN
HHHHCCCCHHHHHHC		37.5022369663
178PhosphorylationFDELQNNSISQERSL
HHHHHHCCCCHHHHH		31.0322369663
180PhosphorylationELQNNSISQERSLEM
HHHHCCCCHHHHHHH		26.3023749301
184PhosphorylationNSISQERSLEMINEN
CCCCHHHHHHHHCHH		28.1228889911
210PhosphorylationAVLTQRPSFTELQND
EEHHCCCCCHHHCCC		47.1725521595
212PhosphorylationLTQRPSFTELQNDQD
HHCCCCCHHHCCCCC		39.8725521595
584PhosphorylationAAMEREQSDGEESAV
HHHHHHHCCCCHHHH		42.7121551504
589PhosphorylationEQSDGEESAVASRKS
HHCCCCHHHHHHCHH		24.6021551504
593PhosphorylationGEESAVASRKSSVKE
CCHHHHHHCHHHHHH		33.3730377154
612PhosphorylationSDIGEPVSPLDSSVF
CCCCCCCCCCCHHHH		30.3821440633
616PhosphorylationEPVSPLDSSVFDFMK
CCCCCCCHHHHHHHH		35.7630377154
627PhosphorylationDFMKRVKTDDEVVLE
HHHHHCCCCCHHHHH		45.9130377154
737PhosphorylationVALSRAVSREGLQVL
HHHHHHHHHCCCEEE		24.5123749301
766PhosphorylationDFYLTLSSAESAYKQ
HHHHHHCCHHHHHHH		38.8928889911
769PhosphorylationLTLSSAESAYKQLEA
HHHCCHHHHHHHHHH		36.2928889911
797PhosphorylationGPKYKAKSKSKSNSP
CHHHCCCCCCCCCCC		47.2024961812
799PhosphorylationKYKAKSKSKSNSPAP
HHCCCCCCCCCCCCC		48.7024961812
801PhosphorylationKAKSKSKSNSPAPIS
CCCCCCCCCCCCCCC		50.0221440633
803PhosphorylationKSKSKSNSPAPISAT
CCCCCCCCCCCCCEE		29.5423749301
808PhosphorylationSNSPAPISATTQSNN
CCCCCCCCEECCCCC		20.6424961812
810PhosphorylationSPAPISATTQSNNGI
CCCCCCEECCCCCHH		20.6924961812
811PhosphorylationPAPISATTQSNNGIA
CCCCCEECCCCCHHH		29.7124961812
813PhosphorylationPISATTQSNNGIAAM
CCCEECCCCCHHHHH		30.1224961812
825PhosphorylationAAMLQRHSRKRFQLK
HHHHHHHHHHHHEEC		41.5124961812
Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources

Oops, there are no upstream regulatory protein records of PIF1_YEAST !!
Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference

Oops, there are no descriptions of PTM sites of PIF1_YEAST !!
Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10) 		Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of PIF1_YEAST !!
Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions
RIM2_YEASTRIM2genetic
7891656
RAD50_YEASTRAD50genetic
15811632
MRE11_YEASTMRE11genetic
15811632
RRM3_YEASTRRM3genetic
15907372
RIR1_YEASTRNR1genetic
15907372
RRM3_YEASTRRM3genetic
15829566
SML1_YEASTSML1genetic
15829566
RAD1_YEASTRAD1genetic
15687264
RAD10_YEASTRAD10genetic
15687264
DPOD3_YEASTPOL32genetic
16537895
TOP3_YEASTTOP3genetic
16816432
SGS1_YEASTSGS1genetic
16816432
RAD54_YEASTRAD54genetic
16816432
RAD55_YEASTRAD55genetic
16816432
RAD5_YEASTRAD5genetic
16449653
RAD18_YEASTRAD18genetic
16449653
TERT_YEASTEST2genetic
18212041
RAD51_YEASTRAD51genetic
18245359
RAD52_YEASTRAD52genetic
18245359
RAD54_YEASTRAD54genetic
18245359
MRE11_YEASTMRE11genetic
18245359
SGS1_YEASTSGS1genetic
18245359
SUA5_YEASTSUA5genetic
19369944
RRM3_YEASTRRM3genetic
19277716
MPH1_YEASTMPH1genetic
18591428
RAD51_YEASTRAD51genetic
19424434
RAD52_YEASTRAD52genetic
19424434
KU70_YEASTYKU70genetic
20194442
PTPA1_YEASTRRD1genetic
20194442
CIK1_YEASTCIK1genetic
20194442
2A5D_YEASTRTS1genetic
20194442
IRC6_YEASTIRC6genetic
20194442
PEX10_YEASTPEX10genetic
20194442
CTF18_YEASTCTF18genetic
20194442
PP4C_YEASTPPH3genetic
20194442
PP2A4_YEASTPPG1genetic
20194442
MAD3_YEASTMAD3genetic
15514023
MAD2_YEASTMAD2genetic
15514023
BUB3_YEASTBUB3genetic
15514023
BUB2_YEASTBUB2genetic
15514023
CTF8_YEASTCTF8genetic
15514023
CTF18_YEASTCTF18genetic
15514023
DCC1_YEASTDCC1genetic
15514023
TERT_YEASTEST2genetic
19704012
PIF1_YEASTPIF1physical
20795654
EXO1_YEASTEXO1genetic
21045806
FEN1_YEASTRAD27physical
20628185
RAD5_YEASTRAD5genetic
20463880
TERT_YEASTEST2genetic
21666682
RIM1_YEASTRIM1physical
23175612
PCNA_YEASTPOL30physical
24025768
ACON_YEASTACO1genetic
24503898
ATM_YEASTTEL1genetic
24699249
TPS2_YEASTTPS2genetic
27708008
PP4C_YEASTPPH3genetic
27708008
PET8_YEASTPET8genetic
27708008
TLG2_YEASTTLG2genetic
27708008
PSB6_YEASTPRE7genetic
27708008
STN1_YEASTSTN1genetic
27708008
MET30_YEASTMET30genetic
27708008
MCM10_YEASTMCM10genetic
27708008
CDC11_YEASTCDC11genetic
27708008
HRP1_YEASTHRP1genetic
27708008
PSB2_YEASTPUP1genetic
27708008
PSA7_YEASTPRE10genetic
27708008
PSB5_YEASTPRE2genetic
27708008
GPT1_YEASTSCT1genetic
27708008
TEC1_YEASTTEC1genetic
27708008
SMY2_YEASTSMY2genetic
27708008
MRC1_YEASTMRC1genetic
27708008
RL13A_YEASTRPL13Agenetic
27708008
PEX7_YEASTPEX7genetic
27708008
ACL4_YEASTYDR161Wgenetic
27708008
RAD9_YEASTRAD9genetic
27708008
ATU2_YEASTCCC2genetic
27708008
BLM10_YEASTBLM10genetic
27708008
YGB0_YEASTMPO1genetic
27708008
MIG1_YEASTMIG1genetic
27708008
LCL3_YEASTLCL3genetic
27708008
PSA3_YEASTPRE9genetic
27708008
YG5L_YEASTYGR266Wgenetic
27708008
RRM3_YEASTRRM3genetic
27708008
SOL3_YEASTSOL3genetic
27708008
FIS1_YEASTFIS1genetic
27708008
BNR1_YEASTBNR1genetic
27708008
YIU0_YEASTYIR020Cgenetic
27708008
MPCP_YEASTMIR1genetic
27708008
ILM1_YEASTILM1genetic
27708008
ASH1_YEASTASH1genetic
27708008
MRS4_YEASTMRS4genetic
27708008
MLP1_YEASTMLP1genetic
27708008
ACE2_YEASTACE2genetic
27708008
UPS2_YEASTUPS2genetic
27708008
ENT2_YEASTENT2genetic
27708008
TOP3_YEASTTOP3genetic
27708008
NDE1_YEASTNDL1genetic
27708008
ATG23_YEASTATG23genetic
27708008
YM8C_YEASTYMR262Wgenetic
27708008
SPO1_YEASTSPO1genetic
27708008
APP1_YEASTAPP1genetic
27708008
PHO91_YEASTPHO91genetic
27708008
ATP23_YEASTATP23genetic
27708008
DCAM_YEASTSPE2genetic
27708008
PMG3_YEASTGPM3genetic
27708008
HST3_YEASTHST3genetic
27708008
DIA2_YEASTDIA2genetic
27708008
BUD7_YEASTBUD7genetic
27708008
PET20_YEASTPET20genetic
27708008
RBD2_YEASTRBD2genetic
27708008
ATPN_YEASTATP20genetic
27708008
NAA30_YEASTMAK3genetic
27708008
DEF1_YEASTDEF1genetic
26483010
YIP4_YEASTYIP4genetic
26483010
AK_YEASTHOM3genetic
26483010
AP1M1_YEASTAPM1genetic
26483010
ARGR1_YEASTARG80genetic
26483010
CDH1_YEASTCDH1genetic
26483010
GCR1_YEASTGCR1genetic
26483010
GTO3_YEASTGTO3genetic
26483010
PRK1_YEASTPRK1genetic
26483010
RAD10_YEASTRAD10genetic
26483010
SKT5_YEASTSKT5genetic
26483010
SOP4_YEASTSOP4genetic
26483010
UMP1_YEASTUMP1genetic
26483010
KC11_YEASTYCK1genetic
26483010
RRM3_YEASTRRM3genetic
27991904
RRM3_YEASTRRM3genetic
28429714
SUB1_YEASTSUB1physical
28369605
SIZ1_YEASTSIZ1genetic
28637749
PCNA_YEASTPOL30physical
29141206
DPOZ_YEASTREV3genetic
26669261
REV1_YEASTREV1genetic
26669261
DNA2_YEASTDNA2genetic
25637499
KU70_YEASTYKU70genetic
25637499
HRQ1_YEASTHRQ1genetic
24440721
DNA2_YEASTDNA2genetic
28970327
Drug and Disease Associations
Kegg Drug
DrugBank
There are no disease associations of PTM sites.
Regulatory Network of PIF1_YEAST

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Related Literatures of Post-Translational Modification
Phosphorylation
ReferencePubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis.";
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
Mol. Cell. Proteomics 7:1389-1396(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; SER-169; SER-170 ANDSER-584, AND MASS SPECTROMETRY.
"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases.";
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169 AND SER-210, ANDMASS SPECTROMETRY.

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