| UniProt ID | MRC1_YEAST | |
|---|---|---|
| UniProt AC | P25588 | |
| Protein Name | Mediator of replication checkpoint protein 1 | |
| Gene Name | MRC1 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 1096 | |
| Subcellular Localization | Nucleus . Associated with chromatin during S phase. | |
| Protein Description | Required for normal DNA replication. Phosphorylated in response to DNA replication stress. Phosphorylation allows it to mediate the activation of RAD53.. | |
| Protein Sequence | MDDALHALSSLTAKKRTTTYKKVAVPILDENDNTNGNGPNDIDNPPELTGNGFLFANATLNRVKNRLEGKKAPEQNHNNGKDRSENSLPTQLISNLYDGGEELEKSEVKDNSYSEKNVSSSFTQTQRIPVSIQQDKVFNVPIHSVNDGKPTQLIKEDGLVNETSQALKTPLTTGRPGATQRIDSSGATSQTQPIKSIEPQSQIITTSSNHSNALSPKIPIIPTELIGTSPLFQSIQNRGPDTQMDVPPQTAHDEDKTQAIGIPQATHQEQKTQIDTVAQTLQDEVPHTLKIREIQSELASEDSKREKARNVEYKKPQKPIPTKKFFSKESFLADFDDSSSNEDDDIKLENAHPKPVQNDDELHENKSVELNLTDETRINEKRVPLLSSYANNLKREIDSSKCITLDLDSDSDEYGDDDMDSIKLSKDESVLPISQLSKATILNLKARLSKQNQKLSQRPNKSKDPKVDHNVLLNTLRKASRKQILDHQKEVIETKGLKLEDMAKEKEIVENLLEQEILRNKRIRQKEKRREKLEENDFQLNAHDSGSDSGSESSGFALSGNEIADYESSGSENDNRRESDSEKEDDEIILKQKKSHHVKHIINESDSDTEVEAKPKEKADESLPKRIAINLGHYGDNIGEDTDKFQETNVLDTQNIEEVMAERNTIENEVKDDVYVNEEADEAIRRQLIDKEKLQLKQKEKEHEAKIKELKKRGVTNFFEMEAEESEDEWHGIGGADGEGSDDYDSDLEKMIDDYSKNNFNPHEIREMLAAENKEMDIKMINKILYDIKNGGFRNKRAKNSLELELSDDDEDDVLQQYRLKRRELMRKRRLEIGDDAKLVKNPKSSAFFESMVEDIIEYKNPFGAEEEYNLDITSTATDLDTQDNSINVGDNTGNNEQKPVDQKNKKVIISEDFVQKSLSFLKSNNYEDFETDKELSRIQHGNDEAIEDLYTLKQNSSIKSFTNSQTDSTTSKTVNTIIDLEKRPEDEDEVENGDTSLVGVFKHPSIIKSFASRTDINDKFKEGNKTVKILKSYKTVGSSKASITYMGKTRKLIAPKRKTEGSHRYHHDHHNKKMKMKTKTKSNKLFESGQDSFDN | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 9 | Phosphorylation | DDALHALSSLTAKKR HHHHHHHHHCCCCCC | 24.88 | 28889911 | |
| 10 | Phosphorylation | DALHALSSLTAKKRT HHHHHHHHCCCCCCC | 30.50 | 28889911 | |
| 12 | Phosphorylation | LHALSSLTAKKRTTT HHHHHHCCCCCCCCC | 38.08 | 28889911 | |
| 84 | Phosphorylation | HNNGKDRSENSLPTQ CCCCCCCCCCCCCHH | 51.79 | 22369663 | |
| 87 | Phosphorylation | GKDRSENSLPTQLIS CCCCCCCCCCHHHHH | 31.51 | 22369663 | |
| 90 | Phosphorylation | RSENSLPTQLISNLY CCCCCCCHHHHHHHC | 41.10 | 22369663 | |
| 94 | Phosphorylation | SLPTQLISNLYDGGE CCCHHHHHHHCCCCH | 29.40 | 22369663 | |
| 97 | Phosphorylation | TQLISNLYDGGEELE HHHHHHHCCCCHHHH | 19.45 | 22369663 | |
| 112 | Phosphorylation | KSEVKDNSYSEKNVS HHHCCCCCCCCCCCC | 39.92 | 28889911 | |
| 113 | Phosphorylation | SEVKDNSYSEKNVSS HHCCCCCCCCCCCCC | 27.40 | 19779198 | |
| 114 | Phosphorylation | EVKDNSYSEKNVSSS HCCCCCCCCCCCCCC | 41.75 | 28889911 | |
| 116 | Acetylation | KDNSYSEKNVSSSFT CCCCCCCCCCCCCCC | 57.62 | 24489116 | |
| 119 | Phosphorylation | SYSEKNVSSSFTQTQ CCCCCCCCCCCCCCE | 29.65 | 28889911 | |
| 120 | Phosphorylation | YSEKNVSSSFTQTQR CCCCCCCCCCCCCEE | 25.78 | 22369663 | |
| 121 | Phosphorylation | SEKNVSSSFTQTQRI CCCCCCCCCCCCEEC | 25.47 | 22369663 | |
| 123 | Phosphorylation | KNVSSSFTQTQRIPV CCCCCCCCCCEECCC | 32.43 | 22369663 | |
| 144 | Phosphorylation | VFNVPIHSVNDGKPT EECCEEEECCCCCCC | 24.28 | 28889911 | |
| 151 | Phosphorylation | SVNDGKPTQLIKEDG ECCCCCCCEEEECCC | 39.22 | 19515819 | |
| 164 | Phosphorylation | DGLVNETSQALKTPL CCCCCCCHHHHCCCC | 13.79 | 24909858 | |
| 168 | Acetylation | NETSQALKTPLTTGR CCCHHHHCCCCCCCC | 51.62 | 25381059 | |
| 179 | Phosphorylation | TTGRPGATQRIDSSG CCCCCCCCEEECCCC | 25.05 | 19515819 | |
| 184 | Phosphorylation | GATQRIDSSGATSQT CCCEEECCCCCCCCC | 28.44 | 22369663 | |
| 185 | Phosphorylation | ATQRIDSSGATSQTQ CCEEECCCCCCCCCC | 28.79 | 22369663 | |
| 189 | Phosphorylation | IDSSGATSQTQPIKS ECCCCCCCCCCCCCC | 30.60 | 25752575 | |
| 191 | Phosphorylation | SSGATSQTQPIKSIE CCCCCCCCCCCCCCC | 35.76 | 28889911 | |
| 205 | Phosphorylation | EPQSQIITTSSNHSN CCCCCEEECCCCCCC | 23.43 | 24961812 | |
| 206 | Phosphorylation | PQSQIITTSSNHSNA CCCCEEECCCCCCCC | 21.54 | 24961812 | |
| 207 | Phosphorylation | QSQIITTSSNHSNAL CCCEEECCCCCCCCC | 22.09 | 28889911 | |
| 208 | Phosphorylation | SQIITTSSNHSNALS CCEEECCCCCCCCCC | 36.15 | 28132839 | |
| 211 | Phosphorylation | ITTSSNHSNALSPKI EECCCCCCCCCCCCC | 28.22 | 28889911 | |
| 215 | Phosphorylation | SNHSNALSPKIPIIP CCCCCCCCCCCCCCC | 23.03 | 21551504 | |
| 223 | Phosphorylation | PKIPIIPTELIGTSP CCCCCCCHHHHCCCH | 32.77 | 23607784 | |
| 228 | Phosphorylation | IPTELIGTSPLFQSI CCHHHHCCCHHHHHH | 21.85 | 23607784 | |
| 229 | Phosphorylation | PTELIGTSPLFQSIQ CHHHHCCCHHHHHHH | 17.88 | 23607784 | |
| 234 | Phosphorylation | GTSPLFQSIQNRGPD CCCHHHHHHHHCCCC | 20.86 | 30377154 | |
| 242 | Phosphorylation | IQNRGPDTQMDVPPQ HHHCCCCCCCCCCCC | 29.00 | 19515819 | |
| 272 | Phosphorylation | ATHQEQKTQIDTVAQ CCCHHHHHHHHHHHH | 30.37 | 19515819 | |
| 300 | Phosphorylation | EIQSELASEDSKREK HHHHHHCCCHHHHHH | 55.34 | 21440633 | |
| 303 | Phosphorylation | SELASEDSKREKARN HHHCCCHHHHHHHHC | 29.57 | 21440633 | |
| 323 | Acetylation | PQKPIPTKKFFSKES CCCCCCCCCCCCCHH | 42.08 | 25381059 | |
| 330 | Phosphorylation | KKFFSKESFLADFDD CCCCCCHHHHCCCCC | 29.33 | 21440633 | |
| 338 | Phosphorylation | FLADFDDSSSNEDDD HHCCCCCCCCCCCCC | 37.19 | 28889911 | |
| 339 | Phosphorylation | LADFDDSSSNEDDDI HCCCCCCCCCCCCCC | 44.15 | 21440633 | |
| 340 | Phosphorylation | ADFDDSSSNEDDDIK CCCCCCCCCCCCCCC | 48.46 | 21440633 | |
| 367 | Phosphorylation | DELHENKSVELNLTD HHHHCCCEEEECCCC | 32.19 | 22369663 | |
| 373 | Phosphorylation | KSVELNLTDETRINE CEEEECCCCCCCCCC | 30.62 | 28889911 | |
| 387 | Phosphorylation | EKRVPLLSSYANNLK CCCHHHHHHHHHHHH | 29.05 | 27017623 | |
| 388 | Phosphorylation | KRVPLLSSYANNLKR CCHHHHHHHHHHHHH | 28.38 | 28889911 | |
| 389 | Phosphorylation | RVPLLSSYANNLKRE CHHHHHHHHHHHHHH | 15.24 | 27017623 | |
| 404 | Phosphorylation | IDSSKCITLDLDSDS HCCCCCEEEECCCCC | 24.56 | 21440633 | |
| 409 | Phosphorylation | CITLDLDSDSDEYGD CEEEECCCCCCCCCC | 46.43 | 20377248 | |
| 411 | Phosphorylation | TLDLDSDSDEYGDDD EEECCCCCCCCCCCC | 35.97 | 20377248 | |
| 414 | Phosphorylation | LDSDSDEYGDDDMDS CCCCCCCCCCCCHHH | 30.74 | 19795423 | |
| 426 | Acetylation | MDSIKLSKDESVLPI HHHCCCCCCCCCCCH | 75.78 | 22865919 | |
| 429 | Phosphorylation | IKLSKDESVLPISQL CCCCCCCCCCCHHHC | 38.80 | 24909858 | |
| 434 | Phosphorylation | DESVLPISQLSKATI CCCCCCHHHCCHHHH | 24.11 | 19515819 | |
| 437 | Phosphorylation | VLPISQLSKATILNL CCCHHHCCHHHHHHH | 16.77 | 28889911 | |
| 482 | Acetylation | TLRKASRKQILDHQK HHHHHHHHHHHHHHH | 38.91 | 25381059 | |
| 579 | Phosphorylation | ENDNRRESDSEKEDD CCCCCCCCCCCHHHH | 44.62 | 25704821 | |
| 581 | Phosphorylation | DNRRESDSEKEDDEI CCCCCCCCCHHHHHH | 60.90 | 25704821 | |
| 605 | Phosphorylation | VKHIINESDSDTEVE HEEECCCCCCCCCCC | 37.62 | 22369663 | |
| 607 | Phosphorylation | HIINESDSDTEVEAK EECCCCCCCCCCCCC | 56.57 | 22369663 | |
| 609 | Phosphorylation | INESDSDTEVEAKPK CCCCCCCCCCCCCCH | 45.97 | 22369663 | |
| 622 | Phosphorylation | PKEKADESLPKRIAI CHHHCCCCCCCCEEE | 50.98 | 17563356 | |
| 653 | Phosphorylation | QETNVLDTQNIEEVM HHCCCCCCCCHHHHH | 21.25 | 28889911 | |
| 801 | Phosphorylation | RNKRAKNSLELELSD CCCCCCCCEEEECCC | 23.63 | 22369663 | |
| 807 | Phosphorylation | NSLELELSDDDEDDV CCEEEECCCCCHHHH | 29.62 | 22369663 | |
| 818 | Phosphorylation | EDDVLQQYRLKRREL HHHHHHHHHHHHHHH | 12.64 | 19779198 | |
| 845 | Phosphorylation | KLVKNPKSSAFFESM HHCCCCCCHHHHHHH | 28.31 | 27017623 | |
| 846 | Phosphorylation | LVKNPKSSAFFESMV HCCCCCCHHHHHHHH | 34.91 | 27017623 | |
| 851 | Phosphorylation | KSSAFFESMVEDIIE CCHHHHHHHHHHHHH | 23.99 | 27017623 | |
| 882 | Phosphorylation | STATDLDTQDNSINV CCEECCCCCCCCEEC | 45.15 | 28889911 | |
| 911 | Phosphorylation | KNKKVIISEDFVQKS CCCEEEECHHHHHHH | 21.71 | 28889911 | |
| 918 | Phosphorylation | SEDFVQKSLSFLKSN CHHHHHHHHHHHHHC | 16.50 | 28889911 | |
| 920 | Phosphorylation | DFVQKSLSFLKSNNY HHHHHHHHHHHHCCC | 35.31 | 20377248 | |
| 924 | Phosphorylation | KSLSFLKSNNYEDFE HHHHHHHHCCCCCHH | 32.24 | 21440633 | |
| 932 | Phosphorylation | NNYEDFETDKELSRI CCCCCHHCHHHHHHH | 52.98 | 28889911 | |
| 937 | Phosphorylation | FETDKELSRIQHGND HHCHHHHHHHCCCCH | 28.56 | 28889911 | |
| 957 | Phosphorylation | LYTLKQNSSIKSFTN HHHHHCCCCCCCCCC | 30.83 | 28889911 | |
| 958 | Phosphorylation | YTLKQNSSIKSFTNS HHHHCCCCCCCCCCC | 41.40 | 21440633 | |
| 961 | Phosphorylation | KQNSSIKSFTNSQTD HCCCCCCCCCCCCCC | 35.32 | 22369663 | |
| 963 | Phosphorylation | NSSIKSFTNSQTDST CCCCCCCCCCCCCCC | 40.84 | 22369663 | |
| 965 | Phosphorylation | SIKSFTNSQTDSTTS CCCCCCCCCCCCCCC | 31.25 | 22369663 | |
| 967 | Phosphorylation | KSFTNSQTDSTTSKT CCCCCCCCCCCCCCE | 31.57 | 22369663 | |
| 969 | Phosphorylation | FTNSQTDSTTSKTVN CCCCCCCCCCCCEEC | 36.65 | 22369663 | |
| 970 | Phosphorylation | TNSQTDSTTSKTVNT CCCCCCCCCCCEECE | 37.59 | 22369663 | |
| 971 | Phosphorylation | NSQTDSTTSKTVNTI CCCCCCCCCCEECEE | 31.98 | 22369663 | |
| 972 | Phosphorylation | SQTDSTTSKTVNTII CCCCCCCCCEECEEE | 26.81 | 22369663 | |
| 996 | Phosphorylation | DEVENGDTSLVGVFK HHHCCCCCCEEEEEC | 25.92 | 24961812 | |
| 997 | Phosphorylation | EVENGDTSLVGVFKH HHCCCCCCEEEEECC | 26.29 | 21440633 | |
| 1006 | Phosphorylation | VGVFKHPSIIKSFAS EEEECCHHHHHHHHH | 36.34 | 28889911 | |
| 1010 | Phosphorylation | KHPSIIKSFASRTDI CCHHHHHHHHHCCCC | 18.68 | 24909858 | |
| 1013 | Phosphorylation | SIIKSFASRTDINDK HHHHHHHHCCCCCHH | 33.43 | 22369663 | |
| 1015 | Phosphorylation | IKSFASRTDINDKFK HHHHHHCCCCCHHHH | 38.44 | 24909858 | |
| 1026 | Acetylation | DKFKEGNKTVKILKS HHHHCCCCEEEEEEE | 66.60 | 25381059 | |
| 1032 | Acetylation | NKTVKILKSYKTVGS CCEEEEEEEEECCCC | 56.03 | 25381059 | |
| 1035 | Acetylation | VKILKSYKTVGSSKA EEEEEEEECCCCCCC | 43.77 | 25381059 | |
| 1089 | Phosphorylation | KSNKLFESGQDSFDN HHHHHHHCCCCCCCC | 34.13 | 30377154 | |
| 1093 | Phosphorylation | LFESGQDSFDN---- HHHCCCCCCCC---- | 26.98 | 21440633 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of MRC1_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of MRC1_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of MRC1_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87; SER-114; SER-120;SER-121; THR-123; SER-144; SER-164; SER-184; SER-189; THR-191;SER-207; SER-215; SER-229; SER-300; SER-367; THR-373; SER-388;SER-434; SER-437; SER-579; SER-581; SER-605; SER-607; SER-622;SER-801; SER-807; SER-911; SER-918; SER-920; THR-932; SER-937;THR-963; SER-965; SER-969; SER-997; SER-1006 AND SER-1093, AND MASSSPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-605; SER-607 ANDSER-622, AND MASS SPECTROMETRY. | |
| "Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry."; Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-605 AND THR-609, ANDMASS SPECTROMETRY. | |
| "Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae."; Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.; J. Proteome Res. 6:1190-1197(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-605 AND SER-607, ANDMASS SPECTROMETRY. | |
