| UniProt ID | MCM5_YEAST | |
|---|---|---|
| UniProt AC | P29496 | |
| Protein Name | Minichromosome maintenance protein 5 | |
| Gene Name | MCM5 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 775 | |
| Subcellular Localization | Nucleus. Mobilized from the cytoplasm to the nucleus as mitosis is completed. Remains in the nucleus until the initiation of the next round of replication. | |
| Protein Description | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity; specifically the MCM2-MCM5 association is proposed to be reversible and to mediate a open ring conformation which may facilitate DNA loading. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity.. | |
| Protein Sequence | MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYSLTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENTSMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLSIMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDPYIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSIYNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQLSRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGTAKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCIDEFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNIDFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMKRYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRITESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKRRLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 2 | Phosphorylation | ------MSFDRPEIY ------CCCCCCCCC | 35.54 | 28152593 | |
| 10 | Phosphorylation | FDRPEIYSAPVLQGE CCCCCCCCCCCCCCC | 31.58 | 24961812 | |
| 18 | Phosphorylation | APVLQGESPNDDDNT CCCCCCCCCCCCCCH | 35.51 | 24961812 | |
| 32 | Acetylation | TEIIKSFKNFILEFR HHHHHHHHHHHHEEE | 59.02 | 24489116 | |
| 71 | Phosphorylation | NMEHLIGYNEDIYKK EHHHHHCCCHHHHHH | 14.37 | 27017623 | |
| 217 | Phosphorylation | SCLSTIESESSMANE HHHHHHHCHHHHCCC | 38.64 | 30377154 | |
| 225 | Phosphorylation | ESSMANESNIGDEST HHHHCCCCCCCCHHH | 33.29 | 23749301 | |
| 312 | Phosphorylation | NGAGSGRSGGGNGGS CCCCCCCCCCCCCCC | 45.36 | 17563356 | |
| 319 | Phosphorylation | SGGGNGGSGVAIRTP CCCCCCCCCEEEECC | 31.34 | 17563356 | |
| 420 | Phosphorylation | LLLGDPGTAKSQLLK EEECCCCCHHHHHHH | 35.98 | 28889911 | |
| 423 | Phosphorylation | GDPGTAKSQLLKFVE CCCCCHHHHHHHHHH | 24.48 | 28889911 | |
| 427 | Acetylation | TAKSQLLKFVEKVSP CHHHHHHHHHHHHCC | 56.83 | 24489116 | |
| 431 | Acetylation | QLLKFVEKVSPIAVY HHHHHHHHHCCEEEE | 42.59 | 24489116 | |
| 669 | Phosphorylation | SLAKLELSPIAQERH HHHHCCCCHHHHHCC | 12.60 | 30377154 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of MCM5_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of MCM5_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of MCM5_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312 AND SER-319, ANDMASS SPECTROMETRY. | |
