| UniProt ID | TOF1_YEAST | |
|---|---|---|
| UniProt AC | P53840 | |
| Protein Name | Topoisomerase 1-associated factor 1 | |
| Gene Name | TOF1 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 1238 | |
| Subcellular Localization | Nucleus . Associated with chromatin during S phase. | |
| Protein Description | Forms a fork protection complex (FPC) with CSM3 and which is required for chromosome segregation during meiosis and DNA damage repair. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors and protects stalled replication forks against the fork-releasing activity of RRM3 helicase.. | |
| Protein Sequence | MSADLQQGTTNAADFSLTVLRARIALLATAIGGPDYTSQIDPPPYKLGDDCLACLKDLKRWFKLVDDQQKRWDVAMAVAEYRILTDDLLPILIDWENKCSLAAKLAKNNPDHEEFRNKAYYDKIALNCLQLLVLMTWPLIVTEQSSSNQITLYGELKKHQLVYKKTILSMESGKVLRAAIRLALDVIKIDRLSRTPRDNMVLKLVLNFFRNVIAIEPGEFTINTKKSMPKKGITSIDTLPPNVSMDDISLNTVISSFHKNKVFGFLLTLTSSLSKEFDQDFINIPLLEIMFYFTKDVNQELLFPRQFETGTHSKVVNKNESSSANNIVTSAGFELSKLLQKEHQMRKNVIKHTSARHSRFGGLLSIQTPDKTRLTVSGSQALVDEKIALQKLDDSKKWNKRIIKKHQSVAAEGLPNSLLNSQTGKAIFFTESNGKHFKEFINNFIDSGFNILLHSVTNYFTTEQDRMVTLEQVEYLLFFAWFVKYQLLRSKIDNSADIKQVSEALKEVTFILVSSLLRSAYDLKNWTVTHAGMIAFNELLNLVSRTKAAQEEDSTDIEFIVSRLFSDERIQLLSNLPKIGSKYSLQFMKSCIELTHSVLKVLEQYSDDKTLVIEGKSRRQKKFNISEGDITKLIEEENVDRDEALDILTSSLRSIEVNFQKVQANYMTEPVIETYINFLERFRELEDDSIKKVFSFFHRVFVQAKEQALLFRFDLIILLREMLSPDGLDRMSRSRKYVSQFSDYFLARLKKRLKKSPAWFVGLLFPPLHNSEVGFYQRYGEYNVLNNESMYAAPASQFKPIPDEEALPPSILLDMKYGVLVSTLLDDGKTELLDQLLKHITHTLDIFKSWLTVNVNAGKETVNPPNEYFTLTGVLNNDPIFKDKDYRALLLLIGYSIPRKINEPCFLPGTVEVSDLTVSCELVKKYLSTPFETPNGLPSSSYLLRVRSEKDSFSHNEQDGWEGDDDYDYNDPYIVPDDQILSKSDAAYFKDLDNNASDKLKGTKFSKGIARSKKKDKRKRRKGEAKTNLPMFGDQDDERPQTVRERHGVFSKEFISDSEDDEDLMNPIFFENETYMRWLLDKNNGQLTEDRYIQFAKFAAERMNNGGVVTGDYTSLFGGSIPSIESIRATESSSFAPDKSLISLASHVASEMSIFDVNNNNNNQLSDDDVNSESRNSLGSSQPSNSQNMFQSEVYSRKESTKRSLEASAADESDEDEEAIRLFGKKSRVVLSQGDSDD | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 2 | Phosphorylation | ------MSADLQQGT ------CCCCCCCCC | 41.62 | 28889911 | |
| 10 | Phosphorylation | ADLQQGTTNAADFSL CCCCCCCCCHHHHHH | 29.68 | 28889911 | |
| 16 | Phosphorylation | TTNAADFSLTVLRAR CCCHHHHHHHHHHHH | 24.55 | 28889911 | |
| 18 | Phosphorylation | NAADFSLTVLRARIA CHHHHHHHHHHHHHH | 19.12 | 28889911 | |
| 85 | Phosphorylation | VAEYRILTDDLLPIL HHHHHHCCCCCHHHH | 25.70 | 28889911 | |
| 100 | Phosphorylation | IDWENKCSLAAKLAK CCCCCHHHHHHHHHH | 23.74 | 28889911 | |
| 163 | Phosphorylation | LKKHQLVYKKTILSM HHHHHHEEEEEEEEC | 18.50 | 28889911 | |
| 166 | Phosphorylation | HQLVYKKTILSMESG HHHEEEEEEEECCCH | 24.01 | 21551504 | |
| 169 | Phosphorylation | VYKKTILSMESGKVL EEEEEEEECCCHHHH | 19.36 | 29734811 | |
| 172 | Phosphorylation | KTILSMESGKVLRAA EEEEECCCHHHHHHH | 34.96 | 29734811 | |
| 234 | Phosphorylation | SMPKKGITSIDTLPP CCCCCCCCCCCCCCC | 28.44 | 27017623 | |
| 249 | Phosphorylation | NVSMDDISLNTVISS CCCHHHCCHHHHHHH | 23.66 | 27017623 | |
| 256 | Phosphorylation | SLNTVISSFHKNKVF CHHHHHHHHHHCCHH | 21.90 | 27017623 | |
| 379 | Phosphorylation | TRLTVSGSQALVDEK CEEEEECCHHHHCHH | 12.55 | 27017623 | |
| 562 | Phosphorylation | TDIEFIVSRLFSDER CCHHHHHHHHCCHHH | 20.60 | 19779198 | |
| 566 | Phosphorylation | FIVSRLFSDERIQLL HHHHHHCCHHHHHHH | 43.06 | 19779198 | |
| 622 | Acetylation | GKSRRQKKFNISEGD CCCHHHHCCCCCCCH | 34.99 | 25381059 | |
| 626 | Phosphorylation | RQKKFNISEGDITKL HHHCCCCCCCHHHHH | 36.31 | 21551504 | |
| 631 | Phosphorylation | NISEGDITKLIEEEN CCCCCHHHHHHHHHC | 25.25 | 30377154 | |
| 654 | Phosphorylation | ILTSSLRSIEVNFQK HHHHHHCCEEECCHH | 28.52 | 25752575 | |
| 674 | Phosphorylation | MTEPVIETYINFLER CCHHHHHHHHHHHHH | 20.69 | 27017623 | |
| 675 | Phosphorylation | TEPVIETYINFLERF CHHHHHHHHHHHHHH | 4.89 | 27017623 | |
| 724 | Phosphorylation | ILLREMLSPDGLDRM HHHHHHCCCCCHHHH | 21.45 | 24961812 | |
| 732 | Phosphorylation | PDGLDRMSRSRKYVS CCCHHHHHHHHHHHH | 28.18 | 24961812 | |
| 734 | Phosphorylation | GLDRMSRSRKYVSQF CHHHHHHHHHHHHHH | 26.31 | 24961812 | |
| 817 | Phosphorylation | SILLDMKYGVLVSTL HHHHHHCCCEEEEEC | 13.06 | 27214570 | |
| 823 | Phosphorylation | KYGVLVSTLLDDGKT CCCEEEEECCCCCCH | 24.70 | 27214570 | |
| 926 | Phosphorylation | SCELVKKYLSTPFET EHHHHHHHHCCCCCC | 10.52 | 28889911 | |
| 928 | Phosphorylation | ELVKKYLSTPFETPN HHHHHHHCCCCCCCC | 30.83 | 28889911 | |
| 939 | Phosphorylation | ETPNGLPSSSYLLRV CCCCCCCCCCCEEEE | 36.64 | 28889911 | |
| 940 | Phosphorylation | TPNGLPSSSYLLRVR CCCCCCCCCCEEEEE | 22.51 | 28889911 | |
| 942 | Phosphorylation | NGLPSSSYLLRVRSE CCCCCCCCEEEEECC | 16.23 | 28889911 | |
| 952 | Phosphorylation | RVRSEKDSFSHNEQD EEECCCCCCCCCCCC | 39.45 | 19779198 | |
| 954 | Phosphorylation | RSEKDSFSHNEQDGW ECCCCCCCCCCCCCC | 29.38 | 19779198 | |
| 997 | Phosphorylation | KDLDNNASDKLKGTK HCCCCCCCHHCCCCC | 37.08 | 23749301 | |
| 1056 | Phosphorylation | VFSKEFISDSEDDED CCCHHHCCCCCCCHH | 40.17 | 28889911 | |
| 1058 | Phosphorylation | SKEFISDSEDDEDLM CHHHCCCCCCCHHHC | 35.81 | 28889911 | |
| 1074 | Phosphorylation | PIFFENETYMRWLLD CHHCCCHHHHHHHHC | 33.91 | 27017623 | |
| 1075 | Phosphorylation | IFFENETYMRWLLDK HHCCCHHHHHHHHCC | 4.40 | 27017623 | |
| 1115 | Phosphorylation | VVTGDYTSLFGGSIP EEECCHHHHCCCCCC | 18.57 | 28889911 | |
| 1134 | Phosphorylation | IRATESSSFAPDKSL HCCCCCCCCCCCHHH | 34.41 | 28889911 | |
| 1177 | Phosphorylation | VNSESRNSLGSSQPS CCCCHHHCCCCCCCC | 32.75 | 28889911 | |
| 1180 | Phosphorylation | ESRNSLGSSQPSNSQ CHHHCCCCCCCCCCC | 30.97 | 27017623 | |
| 1181 | Phosphorylation | SRNSLGSSQPSNSQN HHHCCCCCCCCCCCC | 44.29 | 28889911 | |
| 1186 | Phosphorylation | GSSQPSNSQNMFQSE CCCCCCCCCCHHHHH | 27.54 | 23749301 | |
| 1195 | Phosphorylation | NMFQSEVYSRKESTK CHHHHHHHCCHHHHH | 9.94 | 27017623 | |
| 1200 | Phosphorylation | EVYSRKESTKRSLEA HHHCCHHHHHHHHHH | 42.11 | 27017623 | |
| 1204 | Phosphorylation | RKESTKRSLEASAAD CHHHHHHHHHHHCCC | 31.92 | 22369663 | |
| 1208 | Phosphorylation | TKRSLEASAADESDE HHHHHHHHCCCCCCC | 18.02 | 22369663 | |
| 1213 | Phosphorylation | EASAADESDEDEEAI HHHCCCCCCCCHHHH | 47.01 | 22369663 | |
| 1232 | Phosphorylation | KKSRVVLSQGDSDD- CCCEEEECCCCCCC- | 22.10 | 22369663 | |
| 1236 | Phosphorylation | VVLSQGDSDD----- EEECCCCCCC----- | 50.97 | 22369663 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of TOF1_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of TOF1_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of TOF1_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626; SER-654; SER-1134AND SER-1213, AND MASS SPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626, AND MASSSPECTROMETRY. | |
| "Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae."; Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.; J. Proteome Res. 6:1190-1197(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1213, AND MASSSPECTROMETRY. | |
