dbPTM

SIR4_YEAST - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SIR4_YEAST
UniProt AC	P11978
Protein Name	Regulatory protein SIR4
Gene Name	SIR4
Organism	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length	1358
Subcellular Localization	Nucleus.
Protein Description	The proteins SIR1 through SIR4 are required for transcriptional repression of the silent mating type loci, HML and HMR. [PubMed: 18039933 The proteins SIR2 through SIR4 repress mulitple loci by modulating chromatin structure]
Protein Sequence	MPNDNKTPNRSSTPKFTKKPVTPNDKIPEREEKSNEVKTPKIPLFTFAKSKNYSRPSTAIHTSPHQPSDVKPTSHKQLQQPKSSPLKKNNYNSFPHSNLEKISNSKLLSLLRSKTSAGRIESNNPSHDASRSLASFEQTAFSRHAQQQTSTFNSKPVRTIVPISTSQTNNSFLSGVKSLLSEEKIRDYSKEILGINLANEQPVLEKPLKKGSADIGASVISLTKDKSIRKDTVEEKKEEKLNIGKNFAHSDSLSVPKVSAGDSGISPEESKARSPGIAKPNAIQTEVYGINEESTNERLEINQEKPVKLDENSANSTVASALDTNGTSATTETLTSKKIVPSPKKVAIDQDKITLHDEKTLAPSKHQPITSEQKMKEDADLKRMEILKSPHLSKSPADRPQGRRNSRNFSTRDEETTKLAFLVEYEGQENNYNSTSRSTEKKNDMNTSAKNKNGENKKIGKRPPEIMSTEAHVNKVTEETTKQIQSVRIDGRKVLQKVQGESHIDSRNNTLNVTPSKRPQLGEIPNPMKKHKPNEGRTPNISNGTINIQKKLEPKEIVRDILHTKESSNEAKKTIQNPLNKSQNTALPSTHKVTQKKDIKIGTNDLFQVESAPKISSEIDRENVKSKDEPVSKAVESKSLLNLFSNVLKAPFIKSESKPFSSDALSKEKANFLETIASTEKPENKTDKVSLSQPVSASKHEYSDNFPVSLSQPSKKSFANHTEDEQIEKKKICRGRMNTIITHPGKMELVYVSDSDDSSSDNDSLTDLESLSSGESNEIKVTNDLDTSAEKDQIQAGKWFDPVLDWRKSDRELTKNILWRIADKTTYDKETITDLIEQGIPKHSYLSGNPLTSVTNDICSVENYETSSAFFYQQVHKKDRLQYLPLYAVSTFENTNNTEKNDVTNKNINIGKHSQEQNSSSAKPSQIPTVSSPLGFEETKLSTTPTKSNRRVSHSDTNSSKPKNTKENLSKSSWRQEWLANLKLISVSLVDEFPSELSDSDRQIINEKMQLLKDIFANNLKSAISNNFRESDIIILKGEIEDYPMSSEIKIYYNELQNKPDAKKARFWSFMKTQRFVSNMGFDIQKSCEPVSISTSVKPHVVEPEHMADAKIMPKDILQITKKPLMVKNVKPSSPPDVKSLVQLSTMETKTLPEKKQFDSIFNSNKAKIIPGNGKHASENISLSFSRPASYGYFSVGKRVPIVEDRRVKQLDDITDSNTTEILTSVDVLGTHSQTGTQQSNMYTSTQKTELEIDNKDSVTECSKDMKEDGLSFVDIVLSKAASALDEKEKQLAVANEIIRSLSDEVMRNEIRITSLQGDLTFTKKCLENARSQISEKDAKINKLMEKDFQVNKEIKPY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MPNDNKTPNRSSTP -CCCCCCCCCCCCCC	31.86	19779198
12	Phosphorylation	NKTPNRSSTPKFTKK CCCCCCCCCCCCCCC	45.55	21440633
22	Phosphorylation	KFTKKPVTPNDKIPE CCCCCCCCCCCCCCH	25.54	20377248
39	Phosphorylation	EKSNEVKTPKIPLFT HHCCCCCCCCCCCEE	34.68	21440633
57	Phosphorylation	SKNYSRPSTAIHTSP CCCCCCCCCCCCCCC	29.39	27017623
62	Phosphorylation	RPSTAIHTSPHQPSD CCCCCCCCCCCCCCC	37.53	17287358
63	Phosphorylation	PSTAIHTSPHQPSDV CCCCCCCCCCCCCCC	13.26	21440633
83	Phosphorylation	KQLQQPKSSPLKKNN HHCCCCCCCCCCCCC	43.82	20377248
84	Phosphorylation	QLQQPKSSPLKKNNY HCCCCCCCCCCCCCC	40.14	20377248
91	Phosphorylation	SPLKKNNYNSFPHSN CCCCCCCCCCCCCCC	22.90	28889911
93	Phosphorylation	LKKNNYNSFPHSNLE CCCCCCCCCCCCCHH	29.78	30377154
135	Phosphorylation	DASRSLASFEQTAFS HHHHHHHHHHHHHHH	34.31	30377154
168	Phosphorylation	VPISTSQTNNSFLSG EECCCCCCCCCHHHH	35.81	24961812
171	Phosphorylation	STSQTNNSFLSGVKS CCCCCCCCHHHHHHH	29.63	28889911
174	Phosphorylation	QTNNSFLSGVKSLLS CCCCCHHHHHHHHCC	39.26	24961812
177	Acetylation	NSFLSGVKSLLSEEK CCHHHHHHHHCCHHH	38.62	25381059
181	Phosphorylation	SGVKSLLSEEKIRDY HHHHHHCCHHHHHHH	49.34	30377154
218	Phosphorylation	GSADIGASVISLTKD CCCCCCCEEEHHHCC	18.12	30377154
221	Phosphorylation	DIGASVISLTKDKSI CCCCEEEHHHCCCCC	28.03	30377154
250	Phosphorylation	IGKNFAHSDSLSVPK CCCCCCCCCCCCCCC	26.01	30377154
252	Phosphorylation	KNFAHSDSLSVPKVS CCCCCCCCCCCCCCC	26.09	21551504
254	Phosphorylation	FAHSDSLSVPKVSAG CCCCCCCCCCCCCCC	40.08	30377154
266	Phosphorylation	SAGDSGISPEESKAR CCCCCCCCHHHHHCC	29.77	28889911
274	Phosphorylation	PEESKARSPGIAKPN HHHHHCCCCCCCCCC	32.13	25752575
285	Phosphorylation	AKPNAIQTEVYGINE CCCCCEEEEEECCCC	22.70	19779198
342	Phosphorylation	TSKKIVPSPKKVAID CCCCCCCCCCEEEEC	38.16	25752575
389	Phosphorylation	KRMEILKSPHLSKSP HHHHHHHCCCCCCCC	17.69	19823750
393	Phosphorylation	ILKSPHLSKSPADRP HHHCCCCCCCCCCCC	28.22	19779198
395	Phosphorylation	KSPHLSKSPADRPQG HCCCCCCCCCCCCCC	23.19	28889911
502	Phosphorylation	LQKVQGESHIDSRNN HHHHCCCCCCCCCCC	32.35	27017623
506	Phosphorylation	QGESHIDSRNNTLNV CCCCCCCCCCCEEEC	35.98	28889911
514	Phosphorylation	RNNTLNVTPSKRPQL CCCEEECCCCCCCCC	22.29	22369663
516	Phosphorylation	NTLNVTPSKRPQLGE CEEECCCCCCCCCCC	31.75	28889911
567	Phosphorylation	DILHTKESSNEAKKT HHHHCCCCCHHHHHH	39.77	28889911
582	Phosphorylation	IQNPLNKSQNTALPS HCCCCCHHCCCCCCC	27.82	19779198
585	Phosphorylation	PLNKSQNTALPSTHK CCCHHCCCCCCCCCC	23.18	19779198
589	Phosphorylation	SQNTALPSTHKVTQK HCCCCCCCCCCCCCC	43.95	19779198
611	Phosphorylation	NDLFQVESAPKISSE CCCEEECCCCCCCCC	51.52	22369663
637	Phosphorylation	PVSKAVESKSLLNLF CCHHHHHCHHHHHHH	22.42	21440633
639	Phosphorylation	SKAVESKSLLNLFSN HHHHHCHHHHHHHHH	47.57	22369663
661	Phosphorylation	KSESKPFSSDALSKE CCCCCCCCCCCCCHH	35.52	27214570
690	Phosphorylation	ENKTDKVSLSQPVSA CCCCCCCCCCCCCCC	28.22	21440633
692	Phosphorylation	KTDKVSLSQPVSASK CCCCCCCCCCCCCCC	25.57	25521595
696	Phosphorylation	VSLSQPVSASKHEYS CCCCCCCCCCCCCCC	33.24	30377154
709	Phosphorylation	YSDNFPVSLSQPSKK CCCCCCCCCCCCCCC	23.65	21440633
711	Phosphorylation	DNFPVSLSQPSKKSF CCCCCCCCCCCCCCC	32.32	20377248
714	Phosphorylation	PVSLSQPSKKSFANH CCCCCCCCCCCCCCC	45.11	21440633
717	Phosphorylation	LSQPSKKSFANHTED CCCCCCCCCCCCCCH	33.07	30377154
787	Phosphorylation	KVTNDLDTSAEKDQI EEECCCCCHHHHHHH	36.81	22369663
788	Phosphorylation	VTNDLDTSAEKDQIQ EECCCCCHHHHHHHH	33.81	22369663
925	Phosphorylation	NSSSAKPSQIPTVSS CCCCCCHHHCCCCCC	39.78	24961812
929	Phosphorylation	AKPSQIPTVSSPLGF CCHHHCCCCCCCCCC	34.35	28889911
931	Phosphorylation	PSQIPTVSSPLGFEE HHHCCCCCCCCCCCC	28.81	21440633
932	Phosphorylation	SQIPTVSSPLGFEET HHCCCCCCCCCCCCC	21.58	20377248
939	Phosphorylation	SPLGFEETKLSTTPT CCCCCCCCCCCCCCC	30.04	24961812
943	Phosphorylation	FEETKLSTTPTKSNR CCCCCCCCCCCCCCC	47.43	28889911
944	Phosphorylation	EETKLSTTPTKSNRR CCCCCCCCCCCCCCC	26.11	25521595
946	Phosphorylation	TKLSTTPTKSNRRVS CCCCCCCCCCCCCCC	45.04	28889911
953	Phosphorylation	TKSNRRVSHSDTNSS CCCCCCCCCCCCCCC	18.57	28889911
957	Phosphorylation	RRVSHSDTNSSKPKN CCCCCCCCCCCCCCC	39.76	28889911
961	Acetylation	HSDTNSSKPKNTKEN CCCCCCCCCCCHHHH	60.89	25381059
983	Ubiquitination	QEWLANLKLISVSLV HHHHHHCCEEEEEHH	43.51	15699485
1008	Ubiquitination	DRQIINEKMQLLKDI HHHHHHHHHHHHHHH	27.71	15699485
1013	Ubiquitination	NEKMQLLKDIFANNL HHHHHHHHHHHHHHH	58.32	15699485
1037	Ubiquitination	ESDIIILKGEIEDYP HHCEEEEECCCCCCC	44.05	15699485
1043	Phosphorylation	LKGEIEDYPMSSEIK EECCCCCCCCCCEEE	6.50	28132839
1053	Phosphorylation	SSEIKIYYNELQNKP CCEEEEEHHHHHCCC	12.97	28132839
1087	Phosphorylation	MGFDIQKSCEPVSIS CCCEEECCCCEEEEE	13.82	27017623
1092	Phosphorylation	QKSCEPVSISTSVKP ECCCCEEEEECCCCC	22.87	27017623
1094	Phosphorylation	SCEPVSISTSVKPHV CCCEEEEECCCCCCC	13.71	27017623
1128	Ubiquitination	TKKPLMVKNVKPSSP CCCCEEEECCCCCCC	41.90	15542864
1128	Sumoylation	TKKPLMVKNVKPSSP CCCCEEEECCCCCCC	41.90	15542864
1128	Sumoylation	TKKPLMVKNVKPSSP CCCCEEEECCCCCCC	41.90	-
1133	Phosphorylation	MVKNVKPSSPPDVKS EEECCCCCCCCCHHH	50.16	20377248
1134	Phosphorylation	VKNVKPSSPPDVKSL EECCCCCCCCCHHHH	49.32	17330950
1140	Phosphorylation	SSPPDVKSLVQLSTM CCCCCHHHHHHHHHC	33.35	28889911
1215	Phosphorylation	VKQLDDITDSNTTEI CCCHHHCCCCCCCEE	40.13	29688323
1217	Phosphorylation	QLDDITDSNTTEILT CHHHCCCCCCCEEEE	27.68	29688323
1219	Phosphorylation	DDITDSNTTEILTSV HHCCCCCCCEEEEEE	29.74	29688323
1220	Phosphorylation	DITDSNTTEILTSVD HCCCCCCCEEEEEEE	25.86	29688323
1224	Phosphorylation	SNTTEILTSVDVLGT CCCCEEEEEEEEEEC	32.16	29688323
1225	Phosphorylation	NTTEILTSVDVLGTH CCCEEEEEEEEEECC	16.93	29688323
1231	Phosphorylation	TSVDVLGTHSQTGTQ EEEEEEECCCCCCCH	17.75	29688323
1233	Phosphorylation	VDVLGTHSQTGTQQS EEEEECCCCCCCHHH	29.31	29688323
1235	Phosphorylation	VLGTHSQTGTQQSNM EEECCCCCCCHHHCC	45.25	29688323
1237	Phosphorylation	GTHSQTGTQQSNMYT ECCCCCCCHHHCCEE	27.16	29688323
1240	Phosphorylation	SQTGTQQSNMYTSTQ CCCCCHHHCCEECCC	17.47	29688323
1243	Phosphorylation	GTQQSNMYTSTQKTE CCHHHCCEECCCEEE	11.20	29688323
1244	Phosphorylation	TQQSNMYTSTQKTEL CHHHCCEECCCEEEE	17.96	29688323
1245	Phosphorylation	QQSNMYTSTQKTELE HHHCCEECCCEEEEE	16.02	29688323
1246	Phosphorylation	QSNMYTSTQKTELEI HHCCEECCCEEEEEE	26.70	29688323
1283	Phosphorylation	IVLSKAASALDEKEK HHHHHHHHCCCHHHH	33.94	30377154

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	SAN1	P22470	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of SIR4_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SIR4_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PMM_YEAST	SEC53	physical	11805837
SIR3_YEAST	SIR3	physical	11805837
BLM10_YEAST	BLM10	physical	11805837
IMA1_YEAST	SRP1	physical	11805837
SIR2_YEAST	SIR2	physical	11805837
SIR2_YEAST	SIR2	physical	8752220
SIR2_YEAST	SIR2	physical	9122169
SIR3_YEAST	SIR3	physical	8752220
SIR3_YEAST	SIR3	physical	9122169
UBP3_YEAST	UBP3	physical	8752220
SIR2_YEAST	SIR2	physical	12024030
SIR2_YEAST	SIR2	physical	11500379
RAP1_YEAST	RAP1	physical	7744964
SIR3_YEAST	SIR3	physical	12791253
RAP1_YEAST	RAP1	physical	12080091
H4_YEAST	HHF1	physical	8647429
H3_YEAST	HHT1	physical	8647429
H4_YEAST	HHF1	physical	7867066
H3_YEAST	HHT1	physical	7867066
ESC1_YEAST	ESC1	physical	12417731
SIR4_YEAST	SIR4	physical	10757754
ZDS2_YEAST	ZDS2	physical	14742666
KU80_YEAST	YKU80	physical	14551211
SIR2_YEAST	SIR2	physical	15282295
SIR3_YEAST	SIR3	physical	15282295
SIR4_YEAST	SIR4	physical	1946372
SIR3_YEAST	SIR3	genetic	9742128
RAD52_YEAST	RAD52	genetic	9278054
SAN1_YEAST	SAN1	genetic	2471670
SUM1_YEAST	SUM1	genetic	3905506
NEJ1_YEAST	NEJ1	genetic	11676923
SAN1_YEAST	SAN1	genetic	15078868
SIR3_YEAST	SIR3	genetic	3325825
SIR3_YEAST	SIR3	physical	16554755
GSP1_YEAST	GSP1	physical	16956377
SIR2_YEAST	SIR2	physical	16956377
RT106_YEAST	RTT106	physical	17410207
MPS3_YEAST	MPS3	physical	18039933
SIR4_YEAST	SIR4	physical	14636601
KU70_YEAST	YKU70	genetic	19047366
SIR4_YEAST	SIR4	physical	19217406
SIR2_YEAST	SIR2	physical	19217406
SIR3_YEAST	SIR3	physical	19217406
H2A1_YEAST	HTA1	physical	19217406
H2B1_YEAST	HTB1	physical	19217406
H3_YEAST	HHT1	physical	19217406
H4_YEAST	HHF1	physical	19217406
SIR3_YEAST	SIR3	genetic	3023863
SIR2_YEAST	SIR2	physical	19782027
SUM1_YEAST	SUM1	physical	18086891
NMA2_YEAST	NMA2	physical	18086891
CHD1_YEAST	CHD1	physical	18086891
ESC1_YEAST	ESC1	physical	18086891
NU157_YEAST	NUP157	physical	18086891
CSM1_YEAST	CSM1	physical	21664583
CHZ1_YEAST	CHZ1	physical	20008511
RRS1_YEAST	RRS1	physical	21822217
EBP2_YEAST	EBP2	physical	21822217
MPS3_YEAST	MPS3	physical	21822217
SIR1_YEAST	SIR1	physical	21822217
SIF2_YEAST	SIF2	physical	21822217
SIR4_YEAST	SIR4	physical	21822217
SIR3_YEAST	SIR3	physical	21822217
RAP1_YEAST	RAP1	physical	21822217
KU70_YEAST	YKU70	physical	21822217
ESC1_YEAST	ESC1	physical	21822217
SIR2_YEAST	SIR2	physical	21822217
SIR3_YEAST	SIR3	physical	21896656
SIR2_YEAST	SIR2	physical	21896656
SIR2_YEAST	SIR2	physical	22654676
SIR3_YEAST	SIR3	physical	22654676
H4_YEAST	HHF1	physical	22654676
KU80_YEAST	YKU80	physical	22654676
SIR1_YEAST	SIR1	physical	22654676
SIF2_YEAST	SIF2	physical	22654676
SIR1_YEAST	SIR1	genetic	22654676
NTR2_YEAST	NTR2	physical	22875988
NUF2_YEAST	NUF2	physical	22875988
SMT3_YEAST	SMT3	genetic	23417015
RAP1_YEAST	RAP1	genetic	23417015
SIR3_YEAST	SIR3	physical	23299941
SIR2_YEAST	SIR2	physical	23299941
NU170_YEAST	NUP170	physical	23452847
SIR2_YEAST	SIR2	physical	26319015
NSE3_YEAST	NSE3	physical	27564449
SIR1_YEAST	SIR1	genetic	28188183
SIR1_YEAST	SIR1	physical	28188183
DOT1_YEAST	DOT1	genetic	26587833
NU170_YEAST	NUP170	physical	28883038
PO152_YEAST	POM152	physical	28883038
NU192_YEAST	NUP192	physical	28883038
NU157_YEAST	NUP157	physical	28883038
NU188_YEAST	NUP188	physical	28883038
NIC96_YEAST	NIC96	physical	28883038
NU145_YEAST	NUP145	physical	28883038
NU133_YEAST	NUP133	physical	28883038
NUP84_YEAST	NUP84	physical	28883038

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SIR4_YEAST

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-274; SER-342; SER-692;SER-709; SER-711; SER-788 AND SER-1134, AND MASS SPECTROMETRY.	18407956 [Abstract]
"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1134, AND MASSSPECTROMETRY.	17563356 [Abstract]
"Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry."; Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-62, AND MASSSPECTROMETRY.	17287358 [Abstract]
Sumoylation
Reference	PubMed
"A proteomic strategy for gaining insights into protein sumoylation inyeast."; Denison C., Rudner A.D., Gerber S.A., Bakalarski C.E., Moazed D.,Gygi S.P.; Mol. Cell. Proteomics 4:246-254(2005). Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-1128, AND MASS SPECTROMETRY.	15542864 [Abstract]