| UniProt ID | RAD53_YEAST | |
|---|---|---|
| UniProt AC | P22216 | |
| Protein Name | Serine/threonine-protein kinase RAD53 | |
| Gene Name | RAD53 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 821 | |
| Subcellular Localization | Nucleus . | |
| Protein Description | Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1.. | |
| Protein Sequence | MENITQPTQQSTQATQRFLIEKFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSIKKVWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSNQLLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDRIRSNLKNTSKIASPGLTSSTASSMVANKTGIFKDFSIIDEVVGQGAFATVKKAIERTTGKTFAVKIISKRKVIGNMDGVTRELEVLQKLNHPRIVRLKGFYEDTESYYMVMEFVSGGDLMDFVAAHGAVGEDAGREISRQILTAIKYIHSMGISHRDLKPDNILIEQDDPVLVKITDFGLAKVQGNGSFMKTFCGTLAYVAPEVIRGKDTSVSPDEYEERNEYSSLVDMWSMGCLVYVILTGHLPFSGSTQDQLYKQIGRGSYHEGPLKDFRISEEARDFIDSLLQVDPNNRSTAAKALNHPWIKMSPLGSQSYGDFSQISLSQSLSQQKLLENMDDAQYEFVKAQRKLQMEQQLQEQDQEDQDGKIQGFKIPAHAPIRYTQPKSIEAETREQKLLHSNNTENVKSSKKKGNGRFLTLKPLPDSIIQESLEIQQGVNPFFIGRSEDCNCKIEDNRLSRVHCFIFKKRHAVGKSMYESPAQGLDDIWYCHTGTNVSYLNNNRMIQGTKFLLQDGDEIKIIWDKNNKFVIGFKVEINDTTGLFNEGLGMLQEQRVVLKQTAEEKDLVKKLTQMMAAQRANQPSASSSSMSAKKPPVSDTNNNGNNSVLNDLVESPINANTGNILKRIHSVSLSQSQIDPSKKVKRAKLDQTSKGPENLQFS | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 5 | Phosphorylation | ---MENITQPTQQST ---CCCCCCCCHHHH | 39.83 | 30377154 | |
| 8 | Phosphorylation | MENITQPTQQSTQAT CCCCCCCCHHHHHHH | 29.44 | 30377154 | |
| 11 | Phosphorylation | ITQPTQQSTQATQRF CCCCCHHHHHHHHHH | 16.81 | 30377154 | |
| 12 | Phosphorylation | TQPTQQSTQATQRFL CCCCHHHHHHHHHHH | 20.20 | 30377154 | |
| 15 | Phosphorylation | TQQSTQATQRFLIEK CHHHHHHHHHHHHHH | 15.51 | 30377154 | |
| 24 | Phosphorylation | RFLIEKFSQEQIGEN HHHHHHCCHHHHCCC | 43.82 | 28889911 | |
| 49 | Phosphorylation | QIPIRDLSADISQVL CCCHHHCCCCHHHHH | 28.34 | 28889911 | |
| 53 | Phosphorylation | RDLSADISQVLKEKR HHCCCCHHHHHHHCC | 18.13 | 30377154 | |
| 165 | Phosphorylation | NKVDRIRSNLKNTSK CCHHHHHHHCCCCCC | 44.11 | 17287358 | |
| 170 | Phosphorylation | IRSNLKNTSKIASPG HHHHCCCCCCCCCCC | 29.79 | 28889911 | |
| 171 | Phosphorylation | RSNLKNTSKIASPGL HHHCCCCCCCCCCCC | 31.30 | 28889911 | |
| 175 | Phosphorylation | KNTSKIASPGLTSST CCCCCCCCCCCCHHH | 23.99 | 25521595 | |
| 179 | Phosphorylation | KIASPGLTSSTASSM CCCCCCCCHHHHHHH | 26.92 | 30377154 | |
| 180 | Phosphorylation | IASPGLTSSTASSMV CCCCCCCHHHHHHHH | 30.63 | 30377154 | |
| 181 | Phosphorylation | ASPGLTSSTASSMVA CCCCCCHHHHHHHHH | 23.82 | 28889911 | |
| 182 | Phosphorylation | SPGLTSSTASSMVAN CCCCCHHHHHHHHHC | 30.26 | 28889911 | |
| 184 | Phosphorylation | GLTSSTASSMVANKT CCCHHHHHHHHHCCC | 21.03 | 28889911 | |
| 185 | Phosphorylation | LTSSTASSMVANKTG CCHHHHHHHHHCCCC | 18.14 | 28889911 | |
| 198 | Phosphorylation | TGIFKDFSIIDEVVG CCCCCCCHHHHHHHC | 28.86 | 28889911 | |
| 305 | Phosphorylation | EISRQILTAIKYIHS HHHHHHHHHHHHHHH | 28.15 | 28889911 | |
| 350 | Phosphorylation | AKVQGNGSFMKTFCG EEEECCCCHHHHHHH | 26.60 | 21440633 | |
| 354 | Phosphorylation | GNGSFMKTFCGTLAY CCCCHHHHHHHHHHH | 16.63 | 21440633 | |
| 358 | Phosphorylation | FMKTFCGTLAYVAPE HHHHHHHHHHHHCHH | 14.92 | 30377154 | |
| 372 | Phosphorylation | EVIRGKDTSVSPDEY HHHCCCCCCCCHHHH | 34.22 | 30377154 | |
| 373 | Phosphorylation | VIRGKDTSVSPDEYE HHCCCCCCCCHHHHH | 30.63 | 28889911 | |
| 375 | Phosphorylation | RGKDTSVSPDEYEER CCCCCCCCHHHHHHH | 26.80 | 28889911 | |
| 411 | Phosphorylation | GHLPFSGSTQDQLYK CCCCCCCCCHHHHHH | 23.00 | 28889911 | |
| 424 | Phosphorylation | YKQIGRGSYHEGPLK HHHHCCCCCCCCCCC | 23.19 | 17287358 | |
| 469 | Phosphorylation | NHPWIKMSPLGSQSY CCCCEEECCCCCCCC | 16.39 | 30377154 | |
| 473 | Phosphorylation | IKMSPLGSQSYGDFS EEECCCCCCCCCCHH | 24.52 | 30377154 | |
| 475 | Phosphorylation | MSPLGSQSYGDFSQI ECCCCCCCCCCHHHH | 32.55 | 30377154 | |
| 476 | Phosphorylation | SPLGSQSYGDFSQIS CCCCCCCCCCHHHHC | 17.05 | 30377154 | |
| 480 | Phosphorylation | SQSYGDFSQISLSQS CCCCCCHHHHCCCCC | 31.20 | 30377154 | |
| 483 | Phosphorylation | YGDFSQISLSQSLSQ CCCHHHHCCCCCHHH | 17.84 | 30377154 | |
| 485 | Phosphorylation | DFSQISLSQSLSQQK CHHHHCCCCCHHHHH | 15.78 | 20190278 | |
| 487 | Phosphorylation | SQISLSQSLSQQKLL HHHCCCCCHHHHHHH | 26.98 | 30377154 | |
| 489 | Phosphorylation | ISLSQSLSQQKLLEN HCCCCCHHHHHHHHC | 35.49 | 20190278 | |
| 533 | Acetylation | DGKIQGFKIPAHAPI CCCCCCEEECCCCCC | 55.38 | 25381059 | |
| 543 | Phosphorylation | AHAPIRYTQPKSIEA CCCCCCCCCCCCCCC | 28.66 | 21440633 | |
| 547 | Phosphorylation | IRYTQPKSIEAETRE CCCCCCCCCCCHHHH | 32.14 | 23749301 | |
| 560 | Phosphorylation | REQKLLHSNNTENVK HHHHHHCCCCCCCHH | 31.57 | 25533186 | |
| 563 | Phosphorylation | KLLHSNNTENVKSSK HHHCCCCCCCHHCCC | 32.94 | 28889911 | |
| 568 | Phosphorylation | NNTENVKSSKKKGNG CCCCCHHCCCCCCCC | 43.01 | 28889911 | |
| 569 | Phosphorylation | NTENVKSSKKKGNGR CCCCHHCCCCCCCCC | 42.66 | 28889911 | |
| 635 | Phosphorylation | KRHAVGKSMYESPAQ CCCCCCHHHHCCCCC | 22.22 | 28889911 | |
| 731 | Phosphorylation | KDLVKKLTQMMAAQR HHHHHHHHHHHHHHH | 24.25 | 21440633 | |
| 743 | Phosphorylation | AQRANQPSASSSSMS HHHCCCCCCCCCCCC | 30.90 | 19779198 | |
| 745 | Phosphorylation | RANQPSASSSSMSAK HCCCCCCCCCCCCCC | 34.78 | 21440633 | |
| 746 | Phosphorylation | ANQPSASSSSMSAKK CCCCCCCCCCCCCCC | 26.58 | 28889911 | |
| 747 | Phosphorylation | NQPSASSSSMSAKKP CCCCCCCCCCCCCCC | 27.80 | 21440633 | |
| 748 | Phosphorylation | QPSASSSSMSAKKPP CCCCCCCCCCCCCCC | 20.78 | 23749301 | |
| 750 | Phosphorylation | SASSSSMSAKKPPVS CCCCCCCCCCCCCCC | 38.59 | 23749301 | |
| 757 | Phosphorylation | SAKKPPVSDTNNNGN CCCCCCCCCCCCCCC | 44.48 | 30377154 | |
| 759 | Phosphorylation | KKPPVSDTNNNGNNS CCCCCCCCCCCCCCC | 32.13 | 21440633 | |
| 766 | Phosphorylation | TNNNGNNSVLNDLVE CCCCCCCCCHHHHHH | 31.79 | 20377248 | |
| 774 | Phosphorylation | VLNDLVESPINANTG CHHHHHHCCCCCCCC | 24.49 | 22369663 | |
| 780 | Phosphorylation | ESPINANTGNILKRI HCCCCCCCCHHHHHH | 29.24 | 22369663 | |
| 789 | Phosphorylation | NILKRIHSVSLSQSQ HHHHHHHEEECCHHH | 15.84 | 21551504 | |
| 791 | Phosphorylation | LKRIHSVSLSQSQID HHHHHEEECCHHHCC | 25.89 | 17563356 | |
| 793 | Phosphorylation | RIHSVSLSQSQIDPS HHHEEECCHHHCCCC | 21.93 | 21551504 | |
| 795 | Phosphorylation | HSVSLSQSQIDPSKK HEEECCHHHCCCCHH | 26.23 | 28889911 | |
| 811 | Phosphorylation | KRAKLDQTSKGPENL HHHHCCCCCCCCCCC | 31.88 | 30377154 | |
| 812 | Phosphorylation | RAKLDQTSKGPENLQ HHHCCCCCCCCCCCC | 29.95 | 28889911 | |
| 821 | Phosphorylation | GPENLQFS------- CCCCCCCC------- | 26.76 | 28889911 |
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of RAD53_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of RAD53_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; SER-175; SER-181;THR-182; SER-185; SER-350; THR-354; SER-375; SER-547; SER-560;THR-563; SER-748; SER-774; SER-789; SER-791; SER-793; SER-795; SER-812AND SER-821, AND MASS SPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-424; SER-560; SER-774;SER-789; SER-791 AND SER-793, AND MASS SPECTROMETRY. | |
| "Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry."; Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165 AND SER-424, ANDMASS SPECTROMETRY. | |
