CBPY_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CBPY_YEAST
• UniProt AC: P00729
• Protein Name: Carboxypeptidase Y {ECO:0000303|Ref.4}
• Gene Name: PRC1 {ECO:0000303|PubMed:3028649}
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 532
• Subcellular Localization: Vacuole .
• Protein Description: Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate..
• Protein Sequence: MKAFTSLLCGLGLSTTLAKAISLQRPLGLDKDVLLQAAEKFGLDLDLDHLLKELDSNVLDAWAQIEHLYPNQVMSLETSTKPKFPEAIKTKKDWDFVVKNDAIENYQLRVNKIKDPKILGIDPNVTQYTGYLDVEDEDKHFFFWTFESRNDPAKDPVILWLNGGPGCSSLTGLFFELGPSSIGPDLKPIGNPYSWNSNATVIFLDQPVNVGFSYSGSSGVSNTVAAGKDVYNFLELFFDQFPEYVNKGQDFHIAGESYAGHYIPVFASEILSHKDRNFNLTSVLIGNGLTDPLTQYNYYEPMACGEGGEPSVLPSEECSAMEDSLERCLGLIESCYDSQSVWSCVPATIYCNNAQLAPYQRTGRNVYDIRKDCEGGNLCYPTLQDIDDYLNQDYVKEAVGAEVDHYESCNFDINRNFLFAGDWMKPYHTAVTDLLNQDLPILVYAGDKDFICNWLGNKAWTDVLPWKYDEEFASQKVRNWTASITDEVAGEVKSYKHFTYLRVFNGGHMVPFDVPENALSMVNEWIHGGFSL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
124	N-linked_Glycosylation	KILGIDPNVTQYTGY EECCCCCCCCEEEEE	45.80	28189789
198	N-linked_Glycosylation	NPYSWNSNATVIFLD CCCCCCCCCEEEEEC	36.10	7727362
257	Phosphorylation	DFHIAGESYAGHYIP CEEECCCCCCCCCCH	21.26	22369663
258	Phosphorylation	FHIAGESYAGHYIPV EEECCCCCCCCCCHH	16.58	22369663
262	Phosphorylation	GESYAGHYIPVFASE CCCCCCCCCHHHHHH	13.15	22369663
274	Acetylation	ASEILSHKDRNFNLT HHHHHHCCCCCCCCC	55.82	24489116
279	N-linked_Glycosylation	SHKDRNFNLTSVLIG HCCCCCCCCCEEEEC	46.06	7727362
476	Ubiquitination	DEEFASQKVRNWTAS CHHHHHHHHHHHEEE	40.44	23749301
476	2-Hydroxyisobutyrylation	DEEFASQKVRNWTAS CHHHHHHHHHHHEEE	40.44	-
476	Acetylation	DEEFASQKVRNWTAS CHHHHHHHHHHHEEE	40.44	24489116
479	N-linked_Glycosylation	FASQKVRNWTASITD HHHHHHHHHEEECHH	43.36	7727362
496	Acetylation	AGEVKSYKHFTYLRV CCCCEEECEEEEEEE	39.06	24489116
496	2-Hydroxyisobutyrylation	AGEVKSYKHFTYLRV CCCCEEECEEEEEEE	39.06	-
499	Phosphorylation	VKSYKHFTYLRVFNG CEEECEEEEEEEECC	22.74	19779198
500	Phosphorylation	KSYKHFTYLRVFNGG EEECEEEEEEEECCC	7.49	19779198
520	Phosphorylation	DVPENALSMVNEWIH CCCHHHHHHHHHHHH	20.48	19779198

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	HRD1	Q08109	PMID:22199232
-	K	Ubiquitination	E3 ubiquitin ligase	RSP5	P39940	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
124	N	Phosphorylation	The four high mannose core N-glycans found in mature CPY are Man(11-15)GlcNAc(2) at Asn-124, Man(8-12)GlcNAc(2) at Asn-198, Man(9-14)GlcNAc(2) at Asn-279 and phosphorylated Man(12-17)GlcNAc(2) as well as Man(11-16)GlcNAc(2) at Asn-479.	28189789
198	N	Phosphorylation	The four high mannose core N-glycans found in mature CPY are Man(11-15)GlcNAc(2) at Asn-124, Man(8-12)GlcNAc(2) at Asn-198, Man(9-14)GlcNAc(2) at Asn-279 and phosphorylated Man(12-17)GlcNAc(2) as well as Man(11-16)GlcNAc(2) at Asn-479.	28189789
279	N	Phosphorylation	The four high mannose core N-glycans found in mature CPY are Man(11-15)GlcNAc(2) at Asn-124, Man(8-12)GlcNAc(2) at Asn-198, Man(9-14)GlcNAc(2) at Asn-279 and phosphorylated Man(12-17)GlcNAc(2) as well as Man(11-16)GlcNAc(2) at Asn-479.	28189789
479	N	Phosphorylation	The four high mannose core N-glycans found in mature CPY are Man(11-15)GlcNAc(2) at Asn-124, Man(8-12)GlcNAc(2) at Asn-198, Man(9-14)GlcNAc(2) at Asn-279 and phosphorylated Man(12-17)GlcNAc(2) as well as Man(11-16)GlcNAc(2) at Asn-479.	28189789

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CBPY_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PDR1_YEAST	PDR1	physical	16554755
SIA1_YEAST	SIA1	physical	16554755
ADH1_YEAST	ADH1	physical	16429126
CPYI_YEAST	TFS1	physical	12791700
HRD3_YEAST	HRD3	physical	21486563
OS9_YEAST	YOS9	physical	21486563
DER1_YEAST	DER1	physical	21486563
CBPY_YEAST	PRC1	physical	21486563
CPYI_YEAST	TFS1	physical	12459491
IRE1_YEAST	IRE1	physical	21775630
IRE1_YEAST	IRE1	physical	21852455
HRD3_YEAST	HRD3	physical	21737688
GRP78_YEAST	KAR2	physical	22190740
ENV7_YEAST	ENV7	physical	23166297
GRP78_YEAST	KAR2	physical	22298424
OS9_YEAST	YOS9	physical	22298424
HRD1_YEAST	HRD1	physical	22298424
OS9_YEAST	YOS9	physical	16168370
GRP78_YEAST	KAR2	physical	25036483
OS9_YEAST	YOS9	physical	25036483

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.