| UniProt ID | PBS2_YEAST | |
|---|---|---|
| UniProt AC | P08018 | |
| Protein Name | MAP kinase kinase PBS2 | |
| Gene Name | PBS2 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 668 | |
| Subcellular Localization | Cytoplasm . | |
| Protein Description | Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Seems to phosphorylate HOG1 on a tyrosine residue.. | |
| Protein Sequence | MEDKFANLSLHEKTGKSSIQLNEQTGSDNGSAVKRTSSTSSHYNNINADLHARVKAFQEQRALKRSASVGSNQSEQDKGSSQSPKHIQQIVNKPLPPLPVAGSSKVSQRMSSQVVQASSKSTLKNVLDNQETQNITDVNINIDTTKITATTIGVNTGLPATDITPSVSNTASATHKAQLLNPNRRAPRRPLSTQHPTRPNVAPHKAPAIINTPKQSLSARRGLKLPPGGMSLKMPTKTAQQPQQFAPSPSNKKHIETLSNSKVVEGKRSNPGSLINGVQSTSTSSSTEGPHDTVGTTPRTGNSNNSSNSGSSGGGGLFANFSKYVDIKSGSLNFAGKLSLSSKGIDFSNGSSSRITLDELEFLDELGHGNYGNVSKVLHKPTNVIMATKEVRLELDEAKFRQILMELEVLHKCNSPYIVDFYGAFFIEGAVYMCMEYMDGGSLDKIYDESSEIGGIDEPQLAFIANAVIHGLKELKEQHNIIHRDVKPTNILCSANQGTVKLCDFGVSGNLVASLAKTNIGCQSYMAPERIKSLNPDRATYTVQSDIWSLGLSILEMALGRYPYPPETYDNIFSQLSAIVDGPPPRLPSDKFSSDAQDFVSLCLQKIPERRPTYAALTEHPWLVKYRNQDVHMSEYITERLERRNKILRERGENGLSKNVPALHMGGL | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 9 | Phosphorylation | EDKFANLSLHEKTGK CCCCCCCCCCHHHCC | 27.73 | 22369663 | |
| 14 | Phosphorylation | NLSLHEKTGKSSIQL CCCCCHHHCCCCEEC | 47.02 | 22369663 | |
| 17 | Phosphorylation | LHEKTGKSSIQLNEQ CCHHHCCCCEECCCC | 33.74 | 22369663 | |
| 18 | Phosphorylation | HEKTGKSSIQLNEQT CHHHCCCCEECCCCC | 20.17 | 20377248 | |
| 25 | Phosphorylation | SIQLNEQTGSDNGSA CEECCCCCCCCCCCC | 32.26 | 22369663 | |
| 27 | Phosphorylation | QLNEQTGSDNGSAVK ECCCCCCCCCCCCEE | 31.15 | 22369663 | |
| 31 | Phosphorylation | QTGSDNGSAVKRTSS CCCCCCCCCEEEECC | 35.82 | 22369663 | |
| 36 | Phosphorylation | NGSAVKRTSSTSSHY CCCCEEEECCCCCCC | 22.70 | 29136822 | |
| 37 | Phosphorylation | GSAVKRTSSTSSHYN CCCEEEECCCCCCCC | 35.33 | 29136822 | |
| 38 | Phosphorylation | SAVKRTSSTSSHYNN CCEEEECCCCCCCCC | 31.53 | 17330950 | |
| 39 | Phosphorylation | AVKRTSSTSSHYNNI CEEEECCCCCCCCCC | 33.73 | 29136822 | |
| 40 | Phosphorylation | VKRTSSTSSHYNNIN EEEECCCCCCCCCCC | 19.64 | 29136822 | |
| 41 | Phosphorylation | KRTSSTSSHYNNINA EEECCCCCCCCCCCH | 30.03 | 29136822 | |
| 43 | Phosphorylation | TSSTSSHYNNINADL ECCCCCCCCCCCHHH | 16.32 | 28889911 | |
| 66 | Phosphorylation | EQRALKRSASVGSNQ HHHHHHHHHCCCCCH | 24.25 | 22890988 | |
| 68 | Phosphorylation | RALKRSASVGSNQSE HHHHHHHCCCCCHHH | 28.40 | 25521595 | |
| 71 | Phosphorylation | KRSASVGSNQSEQDK HHHHCCCCCHHHCCC | 29.55 | 25521595 | |
| 74 | Phosphorylation | ASVGSNQSEQDKGSS HCCCCCHHHCCCCCC | 41.28 | 22890988 | |
| 80 | Phosphorylation | QSEQDKGSSQSPKHI HHHCCCCCCCCHHHH | 30.83 | 29136822 | |
| 81 | Phosphorylation | SEQDKGSSQSPKHIQ HHCCCCCCCCHHHHH | 43.26 | 17563356 | |
| 83 | Phosphorylation | QDKGSSQSPKHIQQI CCCCCCCCHHHHHHH | 37.55 | 29136822 | |
| 111 | Phosphorylation | SKVSQRMSSQVVQAS CHHHHHHHHHHHHCC | 21.58 | 22369663 | |
| 112 | Phosphorylation | KVSQRMSSQVVQASS HHHHHHHHHHHHCCC | 20.04 | 21440633 | |
| 118 | Phosphorylation | SSQVVQASSKSTLKN HHHHHHCCCHHHHHH | 22.62 | 22369663 | |
| 119 | Phosphorylation | SQVVQASSKSTLKNV HHHHHCCCHHHHHHH | 33.37 | 21551504 | |
| 212 | Phosphorylation | KAPAIINTPKQSLSA CCCCCCCCCCCCHHH | 22.81 | 27214570 | |
| 238 | Phosphorylation | SLKMPTKTAQQPQQF CCCCCCCCCCCCCCC | 31.98 | 21126336 | |
| 248 | Phosphorylation | QPQQFAPSPSNKKHI CCCCCCCCCCCHHHH | 37.21 | 22369663 | |
| 250 | Phosphorylation | QQFAPSPSNKKHIET CCCCCCCCCHHHHEC | 66.67 | 22369663 | |
| 257 | Phosphorylation | SNKKHIETLSNSKVV CCHHHHECCCCCCEE | 34.58 | 30377154 | |
| 261 | Phosphorylation | HIETLSNSKVVEGKR HHECCCCCCEEECCC | 24.37 | 21440633 | |
| 269 | Phosphorylation | KVVEGKRSNPGSLIN CEEECCCCCCCCEEC | 51.35 | 22369663 | |
| 273 | Phosphorylation | GKRSNPGSLINGVQS CCCCCCCCEECCEEE | 27.60 | 22369663 | |
| 280 | Phosphorylation | SLINGVQSTSTSSST CEECCEEECCCCCCC | 23.17 | 23749301 | |
| 281 | Phosphorylation | LINGVQSTSTSSSTE EECCEEECCCCCCCC | 20.95 | 27017623 | |
| 284 | Phosphorylation | GVQSTSTSSSTEGPH CEEECCCCCCCCCCC | 23.27 | 23749301 | |
| 285 | Phosphorylation | VQSTSTSSSTEGPHD EEECCCCCCCCCCCC | 41.17 | 23749301 | |
| 293 | Phosphorylation | STEGPHDTVGTTPRT CCCCCCCCCCCCCCC | 20.15 | 30377154 | |
| 306 | Phosphorylation | RTGNSNNSSNSGSSG CCCCCCCCCCCCCCC | 34.84 | 28889911 | |
| 307 | Phosphorylation | TGNSNNSSNSGSSGG CCCCCCCCCCCCCCC | 36.82 | 28889911 | |
| 309 | Phosphorylation | NSNNSSNSGSSGGGG CCCCCCCCCCCCCCC | 41.84 | 25315811 | |
| 311 | Phosphorylation | NNSSNSGSSGGGGLF CCCCCCCCCCCCCCC | 26.15 | 23749301 | |
| 312 | Phosphorylation | NSSNSGSSGGGGLFA CCCCCCCCCCCCCCC | 45.01 | 23749301 | |
| 322 | Phosphorylation | GGLFANFSKYVDIKS CCCCCCCEEEEEECC | 23.92 | 27017623 | |
| 329 | Phosphorylation | SKYVDIKSGSLNFAG EEEEEECCCCCEECC | 33.67 | 28889911 | |
| 331 | Phosphorylation | YVDIKSGSLNFAGKL EEEECCCCCEECCEE | 27.51 | 28889911 | |
| 337 | Ubiquitination | GSLNFAGKLSLSSKG CCCEECCEEEECCCC | 31.32 | 17644757 | |
| 339 | Phosphorylation | LNFAGKLSLSSKGID CEECCEEEECCCCCC | 29.68 | 28889911 | |
| 341 | Phosphorylation | FAGKLSLSSKGIDFS ECCEEEECCCCCCCC | 26.99 | 28889911 | |
| 382 | Phosphorylation | SKVLHKPTNVIMATK HHHCCCCCCEEECCC | 47.09 | 19823750 | |
| 388 | Phosphorylation | PTNVIMATKEVRLEL CCCEEECCCCEEHHC | 15.45 | 19823750 | |
| 399 | Acetylation | RLELDEAKFRQILME EHHCCHHHHHHHHHH | 38.75 | 24489116 | |
| 487 | Ubiquitination | NIIHRDVKPTNILCS CCCCCCCCHHCEEEE | 50.87 | 23749301 | |
| 514 | Phosphorylation | VSGNLVASLAKTNIG CCHHHHHHHHHCCCC | 22.75 | 25704821 | |
| 518 | Phosphorylation | LVASLAKTNIGCQSY HHHHHHHCCCCCCCC | 26.85 | 21440633 | |
| 524 | Phosphorylation | KTNIGCQSYMAPERI HCCCCCCCCCCHHHH | 22.43 | 27017623 | |
| 533 | Phosphorylation | MAPERIKSLNPDRAT CCHHHHHHCCCCCCC | 30.89 | 23749301 | |
| 540 | Phosphorylation | SLNPDRATYTVQSDI HCCCCCCCEEEHHHH | 22.18 | 27017623 | |
| 541 | Phosphorylation | LNPDRATYTVQSDIW CCCCCCCEEEHHHHH | 12.15 | 27017623 | |
| 542 | Phosphorylation | NPDRATYTVQSDIWS CCCCCCEEEHHHHHH | 14.02 | 27017623 | |
| 545 | Phosphorylation | RATYTVQSDIWSLGL CCCEEEHHHHHHHHH | 27.08 | 27017623 | |
| 553 | Phosphorylation | DIWSLGLSILEMALG HHHHHHHHHHHHHHC | 24.27 | 27017623 | |
| 593 | Phosphorylation | RLPSDKFSSDAQDFV CCCCCCCCCCHHHHH | 32.80 | 19779198 | |
| 614 | Phosphorylation | IPERRPTYAALTEHP CCCCCCCCHHHHCCC | 7.80 | 21440633 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of PBS2_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of PBS2_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of PBS2_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9; SER-38; SER-248 ANDSER-514, AND MASS SPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-68; SER-71;SER-81; SER-248 AND SER-269, AND MASS SPECTROMETRY. | |
| "Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry."; Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38 AND SER-68, AND MASSSPECTROMETRY. | |
| "Phosphoproteome analysis by mass spectrometry and its application toSaccharomyces cerevisiae."; Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M.,Shabanowitz J., Hunt D.F., White F.M.; Nat. Biotechnol. 20:301-305(2002). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71 AND SER-83, AND MASSSPECTROMETRY. | |
| "Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor."; Maeda T., Takekawa M., Saito H.; Science 269:554-558(1995). Cited for: NUCLEOTIDE SEQUENCE OF 91-101, DOMAIN, PHOSPHORYLATION AT SER-514 ANDTHR-518, AND MUTAGENESIS OF PRO-96; LYS-389; SER-514 AND THR-518. | |
