NCL1_YEAST - dbPTM
NCL1_YEAST - PTM Information in dbPTM
Basic Information of Protein
UniProt ID NCL1_YEAST
UniProt AC P38205
Protein Name Multisite-specific tRNA:(cytosine-C(5))-methyltransferase
Gene Name NCL1
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length 684
Subcellular Localization Nucleus, nucleolus .
Protein Description Methylates cytosine to m5C at several positions in different tRNAs and pre-tRNAs containing intron. Able to modify tRNAs at all four positions (34, 40, 48 and 49) at which m5C has been found in tRNAs. May be involved in ribosome biogenesis as its disruption leads to increased sensitivity to the antibiotic paromomycin..
Protein Sequence MARRKNFKKGNKKTFGARDDSRAQKNWSELVKENEKWEKYYKTLALFPEDQWEEFKKTCQAPLPLTFRITGSRKHAGEVLNLFKERHLPNLTNVEFEGEKIKAPVELPWYPDHLAWQLDVPKTVIRKNEQFAKTQRFLVVENAVGNISRQEAVSMIPPIVLEVKPHHTVLDMCAAPGSKTAQLIEALHKDTDEPSGFVVANDADARRSHMLVHQLKRLNSANLMVVNHDAQFFPRIRLHGNSNNKNDVLKFDRILCDVPCSGDGTMRKNVNVWKDWNTQAGLGLHAVQLNILNRGLHLLKNNGRLVYSTCSLNPIENEAVVAEALRKWGDKIRLVNCDDKLPGLIRSKGVSKWPVYDRNLTEKTKGDEGTLDSFFSPSEEEASKFNLQNCMRVYPHQQNTGGFFITVFEKVEDSTEAATEKLSSETPALESEGPQTKKIKVEEVQKKERLPRDANEEPFVFVDPQHEALKVCWDFYGIDNIFDRNTCLVRNATGEPTRVVYTVCPALKDVIQANDDRLKIIYSGVKLFVSQRSDIECSWRIQSESLPIMKHHMKSNRIVEANLEMLKHLLIESFPNFDDIRSKNIDNDFVEKMTKLSSGCAFIDVSRNDPAKENLFLPVWKGNKCINLMVCKEDTHELLYRIFGIDANAKATPSAEEKEKEKETTESPAETTTGTSTEAPSAAN
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure		 ASA (%) Reference Orthologous
Protein Cluster
25AcetylationRDDSRAQKNWSELVK
CCCHHHHHHHHHHHH		60.4424489116
28PhosphorylationSRAQKNWSELVKENE
HHHHHHHHHHHHHHH		30.3730377154
36AcetylationELVKENEKWEKYYKT
HHHHHHHHHHHHHHH		72.7024489116
56AcetylationEDQWEEFKKTCQAPL
HHHHHHHHHHCCCCC		50.9125381059
84AcetylationGEVLNLFKERHLPNL
HHHHHHHHHHCCCCC		57.9424489116
100AcetylationNVEFEGEKIKAPVEL
EEEEECCEEECCEEC		61.4324489116
154PhosphorylationISRQEAVSMIPPIVL
CCHHHHHHCCCCEEE		20.3129688323
168PhosphorylationLEVKPHHTVLDMCAA
EEECCCCCHHHCCCC		21.5229688323
178PhosphorylationDMCAAPGSKTAQLIE
HCCCCCCCHHHHHHH		26.2729688323
216UbiquitinationHMLVHQLKRLNSANL
HHHHHHHHHCCCCCE		48.5522817900
220PhosphorylationHQLKRLNSANLMVVN
HHHHHCCCCCEEEEC		24.1130377154
245AcetylationLHGNSNNKNDVLKFD
ECCCCCCCCCCEECC		59.7922865919
250AcetylationNNKNDVLKFDRILCD
CCCCCCEECCEEEEE		45.1022865919
268UbiquitinationSGDGTMRKNVNVWKD
CCCCCCCCCEEECCC		55.1923749301
278PhosphorylationNVWKDWNTQAGLGLH
EECCCCCCCCCCCCC		18.6229136822
352AcetylationIRSKGVSKWPVYDRN
HHCCCCCCCCCCCCC		53.7822865919
414PhosphorylationVFEKVEDSTEAATEK
EEEECCCCHHHHHHH		18.0319779198
415PhosphorylationFEKVEDSTEAATEKL
EEECCCCHHHHHHHH		41.8029136822
419PhosphorylationEDSTEAATEKLSSET
CCCHHHHHHHHHCCC		40.4729136822
421AcetylationSTEAATEKLSSETPA
CHHHHHHHHHCCCCC		49.6824489116
423PhosphorylationEAATEKLSSETPALE
HHHHHHHHCCCCCCC		36.8822369663
424PhosphorylationAATEKLSSETPALES
HHHHHHHCCCCCCCC		56.7922369663
426PhosphorylationTEKLSSETPALESEG
HHHHHCCCCCCCCCC		18.7922369663
431PhosphorylationSETPALESEGPQTKK
CCCCCCCCCCCCCCE		49.0222369663
436PhosphorylationLESEGPQTKKIKVEE
CCCCCCCCCEEEHHH		37.1222369663
437AcetylationESEGPQTKKIKVEEV
CCCCCCCCEEEHHHH		47.4524489116
440AcetylationGPQTKKIKVEEVQKK
CCCCCEEEHHHHHHH		53.0922865919
446AcetylationIKVEEVQKKERLPRD
EEHHHHHHHHCCCCC		62.8724489116
508UbiquitinationYTVCPALKDVIQAND
EEECHHHHHHHHCCC		52.6423749301
530PhosphorylationSGVKLFVSQRSDIEC
EEEEEEEECCCCCEE		16.7319779198
592AcetylationIDNDFVEKMTKLSSG
CCHHHHHHHHHHCCC		46.7124489116
606PhosphorylationGCAFIDVSRNDPAKE
CCEEEECCCCCHHHH		22.9426447709
612AcetylationVSRNDPAKENLFLPV
CCCCCHHHHCCEEEE		53.1524489116
654PhosphorylationANAKATPSAEEKEKE
CCCCCCCCHHHHHHH		43.9119779198
664PhosphorylationEKEKEKETTESPAET
HHHHHHCCCCCCCCC		47.1022369663
665PhosphorylationKEKEKETTESPAETT
HHHHHCCCCCCCCCC		35.2422369663
667PhosphorylationKEKETTESPAETTTG
HHHCCCCCCCCCCCC		27.5822369663
671PhosphorylationTTESPAETTTGTSTE
CCCCCCCCCCCCCCC		31.5920377248
672PhosphorylationTESPAETTTGTSTEA
CCCCCCCCCCCCCCC		18.0922369663
673PhosphorylationESPAETTTGTSTEAP
CCCCCCCCCCCCCCC		45.9719795423
675PhosphorylationPAETTTGTSTEAPSA
CCCCCCCCCCCCCCC		30.3122369663
676PhosphorylationAETTTGTSTEAPSAA
CCCCCCCCCCCCCCC		26.1225521595
677PhosphorylationETTTGTSTEAPSAAN
CCCCCCCCCCCCCCC		35.6819779198
681PhosphorylationGTSTEAPSAAN----
CCCCCCCCCCC----		47.2422369663
Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources

Oops, there are no upstream regulatory protein records of NCL1_YEAST !!
Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference

Oops, there are no descriptions of PTM sites of NCL1_YEAST !!
Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10) 		Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of NCL1_YEAST !!
Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions
C1TM_YEASTMIS1physical
11805826
RM10_YEASTMRPL10physical
11805826
RLA4_YEASTRPP2Bphysical
11805826
FBRL_YEASTNOP1physical
16554755
YRA1_YEASTYRA1physical
16554755
MAM33_YEASTMAM33physical
16554755
STM1_YEASTSTM1physical
16554755
RRP6_YEASTRRP6genetic
16387656
PAP2_YEASTPAP2genetic
16387656
CFAH_HUMANCFHphysical
17959597
MET22_YEASTMET22genetic
18443146
XRN2_YEASTRAT1genetic
18443146
XRN1_YEASTXRN1genetic
18443146
PFD6_YEASTYKE2genetic
19061648
SWD3_YEASTSWD3genetic
19061648
TAN1_YEASTTAN1genetic
19061648
PXR1_YEASTPXR1genetic
19061648
PFD5_YEASTGIM5genetic
19061648
SRC1_YEASTSRC1genetic
19061648
IF2B_YEASTSUI3genetic
19061648
SYDM_YEASTMSD1genetic
19061648
PFD2_YEASTGIM4genetic
19061648
PRP19_YEASTPRP19genetic
19061648
LHP1_YEASTLHP1genetic
19061648
RRP8_YEASTRRP8genetic
19061648
ARX1_YEASTARX1genetic
19061648
GIR2_YEASTGIR2genetic
19061648
LRS4_YEASTLRS4genetic
19061648
TRM7_YEASTTRM7genetic
19061648
NGR1_YEASTNGR1genetic
19061648
ARC1_YEASTARC1genetic
19061648
IF4F2_YEASTTIF4632genetic
19061648
LTV1_YEASTLTV1genetic
19061648
PAN3_YEASTPAN3genetic
19061648
MRT4_YEASTMRT4genetic
19061648
TRM11_YEASTTRM11genetic
19061648
HIR2_YEASTHIR2genetic
19061648
CSK22_YEASTCKA2genetic
19061648
UAF30_YEASTUAF30genetic
19061648
BMT5_YEASTBMT5genetic
19061648
NU188_YEASTNUP188genetic
19061648
SAP30_YEASTSAP30genetic
19061648
ELP6_YEASTELP6genetic
19061648
RU2A_YEASTLEA1genetic
19061648
ELP4_YEASTELP4genetic
19061648
SYNC_YEASTDED81genetic
19061648
PP12_YEASTGLC7genetic
19061648
EF1A_YEASTTEF2genetic
22895820
MET22_YEASTMET22genetic
22895820
SLX5_YEASTSLX5genetic
27708008
VMA21_YEASTVMA21genetic
27708008
HPC2_YEASTHPC2genetic
27708008
YB69_YEASTYBR219Cgenetic
27708008
SNF5_YEASTSNF5genetic
27708008
STE50_YEASTSTE50genetic
27708008
RLA1_YEASTRPP1Agenetic
27708008
UBX3_YEASTUBX3genetic
27708008
ATIF_YEASTINH1genetic
27708008
TRMB_YEASTTRM8genetic
27708008
MAF1_YEASTMAF1genetic
27708008
OS9_YEASTYOS9genetic
27708008
RPA14_YEASTRPA14genetic
27708008
TRM82_YEASTTRM82genetic
27708008
MCM21_YEASTMCM21genetic
27708008
GGA1_YEASTGGA1genetic
27708008
DYL1_YEASTDYN2genetic
27708008
AIM11_YEASTAIM11genetic
27708008
ODPA_YEASTPDA1genetic
27708008
SGF73_YEASTSGF73genetic
27708008
YGI1_YEASTYGL081Wgenetic
27708008
MRM2_YEASTMRM2genetic
27708008
XRN1_YEASTXRN1genetic
27708008
DSD1_YEASTDSD1genetic
27708008
VAM7_YEASTVAM7genetic
27708008
TAN1_YEASTTAN1genetic
27708008
PFD3_YEASTPAC10genetic
27708008
TBP7_YEASTYTA7genetic
27708008
HTD2_YEASTHTD2genetic
27708008
URM1_YEASTURM1genetic
27708008
VHR1_YEASTVHR1genetic
27708008
ATG32_YEASTATG32genetic
27708008
PHO86_YEASTPHO86genetic
27708008
RS27A_YEASTRPS27Agenetic
27708008
BPT1_YEASTBPT1genetic
27708008
FPS1_YEASTFPS1genetic
27708008
RNP1_YEASTRNP1genetic
27708008
PFD6_YEASTYKE2genetic
27708008
ADY4_YEASTADY4genetic
27708008
YL283_YEASTYLR283Wgenetic
27708008
PAU4_YEASTPAU4genetic
27708008
TBA3_YEASTTUB3genetic
27708008
YMP0_YEASTYMR010Wgenetic
27708008
AIP1_YEASTAIP1genetic
27708008
HFA1_YEASTHFA1genetic
27708008
TMA23_YEASTTMA23genetic
27708008
YME2_YEASTYME2genetic
27708008
ASI3_YEASTASI3genetic
27708008
PFD4_YEASTGIM3genetic
27708008
IZH2_YEASTIZH2genetic
27708008
BUB3_YEASTBUB3genetic
27708008
BUD7_YEASTBUD7genetic
27708008
MSC6_YEASTMSC6genetic
27708008
MET31_YEASTMET31genetic
27708008
SPEE_YEASTSPE3genetic
27708008
MED1_YEASTMED1genetic
27708008
SYT1_YEASTSYT1genetic
27708008
DOM34_YEASTDOM34genetic
29158977
Drug and Disease Associations
Kegg Drug
DrugBank
There are no disease associations of PTM sites.
Regulatory Network of NCL1_YEAST

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Related Literatures of Post-Translational Modification
Phosphorylation
ReferencePubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis.";
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
Mol. Cell. Proteomics 7:1389-1396(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423; SER-424; THR-426AND SER-431, AND MASS SPECTROMETRY.
"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases.";
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-665 AND SER-667, ANDMASS SPECTROMETRY.

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