dbPTM

TRM82_YEAST - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TRM82_YEAST
UniProt AC	Q03774
Protein Name	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82 {ECO:0000255\|HAMAP-Rule:MF_03056}
Gene Name	TRM82 {ECO:0000255\|HAMAP-Rule:MF_03056}
Organism	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length	444
Subcellular Localization	Nucleus .
Protein Description	Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA, a modification required to maintain stability of tRNAs; its absence resulting in tRNA decay. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit..
Protein Sequence	MSVIHPLQNLLTSRDGSLVFAIIKNCILSFKYQSPNHWEFAGKWSDDFDKIQESRNTTAKEQQGQSSENENENKKLKSNKGDSIKRTAAKVPSPGLGAPPIYSYIRNLRLTSDESRLIACADSDKSLLVFDVDKTSKNVLKLRKRFCFSKRPNAISIAEDDTTVIIADKFGDVYSIDINSIPEEKFTQEPILGHVSMLTDVHLIKDSDGHQFIITSDRDEHIKISHYPQCFIVDKWLFGHKHFVSSICCGKDYLLLSAGGDDKIFAWDWKTGKNLSTFDYNSLIKPYLNDQHLAPPRFQNENNDIIEFAVSKIIKSKNLPFVAFFVEATKCIIILEMSEKQKGDLALKQIITFPYNVISLSAHNDEFQVTLDNKESSGVQKNFAKFIEYNLNENSFVVNNEKSNEFDSAIIQSVQGDSNLVTKKEEIYPLYNVSSLRKHGEHYS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
31	Ubiquitination	KNCILSFKYQSPNHW HHHEEEEEECCCCCC	38.72	23749301
50	Acetylation	KWSDDFDKIQESRNT ECCCCHHHHHHHHHC	46.40	24489116
66	Phosphorylation	AKEQQGQSSENENEN HHHHHCCCCCCHHHH	46.58	28889911
67	Phosphorylation	KEQQGQSSENENENK HHHHCCCCCCHHHHH	36.23	17563356
85	Acetylation	SNKGDSIKRTAAKVP CCCCCHHHHHHCCCC	47.88	25381059
87	Phosphorylation	KGDSIKRTAAKVPSP CCCHHHHHHCCCCCC	26.43	22369663
90	Acetylation	SIKRTAAKVPSPGLG HHHHHHCCCCCCCCC	52.82	24489116
93	Phosphorylation	RTAAKVPSPGLGAPP HHHCCCCCCCCCCCC	34.29	22369663
287	Phosphorylation	YNSLIKPYLNDQHLA HHHCCHHHCCCCCCC	17.09	22369663
385	Ubiquitination	GVQKNFAKFIEYNLN CHHHHHHHHHHEECC	42.61	23749301

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of TRM82_YEAST !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of TRM82_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TRM82_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HXKA_YEAST	HXK1	physical	16554755
DUS1_YEAST	DUS1	genetic	16387656
DUS2_YEAST	SMM1	genetic	16387656
MOD5_YEAST	MOD5	genetic	18314501
PUS1_YEAST	PUS1	genetic	18314501
XPOT_YEAST	LOS1	genetic	18314501
TRM10_YEAST	TRM10	genetic	18314501
PT309_YEAST	PET309	genetic	19061648
EIF3J_YEAST	HCR1	genetic	19061648
STP1_YEAST	STP1	genetic	19061648
PUS7_YEAST	PUS7	genetic	19061648
PFD5_YEAST	GIM5	genetic	19061648
PUS1_YEAST	PUS1	genetic	19061648
LRP1_YEAST	LRP1	genetic	19061648
PFD5_YEAST	GIM5	genetic	19547744
IST2_YEAST	IST2	genetic	20093466
MAF1_YEAST	MAF1	genetic	20093466
UBP3_YEAST	UBP3	genetic	20093466
PUS3_YEAST	DEG1	genetic	20093466
FEN1_YEAST	RAD27	genetic	20093466
PNT1_YEAST	PNT1	genetic	20093466
PUS1_YEAST	PUS1	genetic	20093466
CUR1_YEAST	CUR1	genetic	20093466
DUS4_YEAST	DUS4	genetic	16387656
TRM10_YEAST	TRM10	genetic	16387656
NCL1_YEAST	NCL1	genetic	16387656
PUS7_YEAST	PUS7	genetic	16387656
DUS3_YEAST	DUS3	genetic	16387656
CSN12_YEAST	YJR084W	genetic	27708008
RTG3_YEAST	RTG3	genetic	27708008
MTU1_YEAST	SLM3	genetic	27708008
HTD2_YEAST	HTD2	genetic	27708008

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TRM82_YEAST

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93, AND MASSSPECTROMETRY.	18407956 [Abstract]
"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67 AND SER-93, AND MASSSPECTROMETRY.	17563356 [Abstract]