OSH2_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: OSH2_YEAST
• UniProt AC: Q12451
• Protein Name: Oxysterol-binding protein homolog 2
• Gene Name: OSH2
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 1283
• Subcellular Localization: Cell membrane ; Peripheral membrane protein . Cell periphery, enriched in small buds of G1 phase cells and near the bud-neck region of S phase cells.
• Protein Description: Lipid-binding protein involved in maintenance of intracellular sterol distribution and homeostasis. Binds to phosphoinositides..
• Protein Sequence: MSREDLSIAEDLNQVSKPLLKVKLLEVLGQGDFKHLKALVDNEFQPKDDPSVQQVLNLILHYAVQVAPILLIKEIVAHWVDQVGDEKSSSKSDDGIHLDLNYQDENGNTPLHLAAAQSRSDVISFLLSQKSINDCVKNKAHQQPLDMCKDLNVAQMIQLKRDDYFLETVHSLRAAMNKRDFSKLDSIWKNPRNLNLLDINGIDPETGTTLLYEYSQKKDIEMCQWLLKHGAEATVKDGKGRSPLDLVKNIKLPAKPSNNVTPEIKLKNLLEKNLREQAIVHEDVASSKPPTYKGFLKKWTNFAHGYKLRWFILSGDGNLSYYKDQSHVDRPRGTLKVSTCRLHIDSSEKLNFELLGGITGTTRWRLKGNHPIETTRWVNAIQSAIRFAKDKEILNKKKAVPPSLALKNKSPALISHSKTQGSLPEASQYYQHTLHKEVIQPSSVSLYRRPSNNLSVVSSEIQLNDNLTESGKRFVSKMIENRLDGSKTPVGVHTGSALQRVRSSNTLKSNRSMQSGSGVASPIDKVPNGANLSQSNTTTGSTASLSDNNYIDNFEGDEANSDDEEEDLGINFDRDEEYIKAQYGPYKEKLDMYEQAISIELSSLIELIEQEEPSPEVWLTIKKSLINTSTIFGKLKDLTYKRDKRLVDMVSKQGDVNNVWVQSVKELEMELSNKTERLASIDKERRGLKKILHKKLLESHATAGNKESLENDKEQESDTTASTLGQIAKFISATKEEDEASDADEFYDAAELVDEVTELTEAHPEISTAAAPKHAPPPVPNETDNDSQYVQDEKSKIESNVEKTSQKFEKQNNLVTEDEPKTDQSLKNFKAEDKESQVKEKTKEIASSVIGEKTIVAVTTVQKRKEEYLLKEGSYLGYEDGIRKRLSMDKDDRPKISLWAVLKSMVGKDMTRMTLPVTFNEPTSLLQRVAEDLEYSELLDQAATFEDSTLRTLYVAAFTASSYASTTKRVAKPFNPLLGETFEYSRPDKQYRFFTEQVSHHPPISATWTESPRWDFWGESFVDTKFNGRSFNVKHLGLWHIKLRPNDNEKEELYTWKKPNNTVIGILIGNPQVDNHGEVNVVNHTTGDHCKLYFKARGWRSSGAYEITGEVYNKKKQKVWILGGHWNEAIFAKKVVKDGDLSLEKTRTAASAGNGPTDDGTKFLIWKANDRPEEPFNLTPFAITLNAPQPHLLPWLPPTDTRLRPDQRAMEDGRYDEAGDEKFRVEEKQRAARRKREENNLEYHPQWFVRDTHPITKAKYWRYTGKYWVKRRDHDLKDCGDIF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSREDLSIA ------CCHHHHHHH	56.53	22369663
2	Acetylation	------MSREDLSIA ------CCHHHHHHH	56.53	15665377
7	Phosphorylation	-MSREDLSIAEDLNQ -CCHHHHHHHHHHHH	31.88	22369663
16	Phosphorylation	AEDLNQVSKPLLKVK HHHHHHCCHHHHHHH	20.84	22369663
34	Acetylation	VLGQGDFKHLKALVD HHCCCCHHHHHHHHC	53.41	24489116
89	Phosphorylation	QVGDEKSSSKSDDGI HHCCCCCCCCCCCCE	53.50	19779198
90	Phosphorylation	VGDEKSSSKSDDGIH HCCCCCCCCCCCCEE	43.18	19779198
92	Phosphorylation	DEKSSSKSDDGIHLD CCCCCCCCCCCEEEE	42.77	28889911
183	Acetylation	MNKRDFSKLDSIWKN HHHCCHHHHHHHHCC	57.20	24489116
248	Acetylation	RSPLDLVKNIKLPAK CCHHHHHHHCCCCCC	61.32	24489116
255	Ubiquitination	KNIKLPAKPSNNVTP HHCCCCCCCCCCCCH	47.22	23749301
272	Acetylation	KLKNLLEKNLREQAI HHHHHHHHHHHHHHC	62.34	24489116
307	Acetylation	TNFAHGYKLRWFILS CCCCCCCEEEEEEEC	35.85	24489116
323	Acetylation	DGNLSYYKDQSHVDR CCCCCEECCCCCCCC	40.85	24489116
407	Acetylation	VPPSLALKNKSPALI CCHHHHHCCCCCHHH	57.64	25381059
409	Acetylation	PSLALKNKSPALISH HHHHHCCCCCHHHCC	57.12	25381059
410	Phosphorylation	SLALKNKSPALISHS HHHHCCCCCHHHCCC	25.46	29136822
415	Phosphorylation	NKSPALISHSKTQGS CCCCHHHCCCCCCCC	23.66	19779198
417	Phosphorylation	SPALISHSKTQGSLP CCHHHCCCCCCCCCC	30.52	19779198
419	Phosphorylation	ALISHSKTQGSLPEA HHHCCCCCCCCCCHH	40.97	29136822
422	Phosphorylation	SHSKTQGSLPEASQY CCCCCCCCCCHHHHH	30.82	17330950
427	Phosphorylation	QGSLPEASQYYQHTL CCCCCHHHHHHHHHC	19.63	29136822
429	Phosphorylation	SLPEASQYYQHTLHK CCCHHHHHHHHHCCC	11.55	19779198
433	Phosphorylation	ASQYYQHTLHKEVIQ HHHHHHHHCCCCEEC	18.29	19779198
442	Phosphorylation	HKEVIQPSSVSLYRR CCCEECCCCCEEEEC	27.31	22890988
443	Phosphorylation	KEVIQPSSVSLYRRP CCEECCCCCEEEECC	23.55	22890988
445	Phosphorylation	VIQPSSVSLYRRPSN EECCCCCEEEECCCC	22.95	25521595
447	Phosphorylation	QPSSVSLYRRPSNNL CCCCCEEEECCCCCE	9.32	22890988
451	Phosphorylation	VSLYRRPSNNLSVVS CEEEECCCCCEEEEE	36.22	22369663
455	Phosphorylation	RRPSNNLSVVSSEIQ ECCCCCEEEEEEEEE	23.69	25521595
458	Phosphorylation	SNNLSVVSSEIQLND CCCEEEEEEEEECCC	21.79	25521595
459	Phosphorylation	NNLSVVSSEIQLNDN CCEEEEEEEEECCCC	27.40	20377248
470	Phosphorylation	LNDNLTESGKRFVSK CCCCCCHHHHHHHHH	44.36	19779198
477	Acetylation	SGKRFVSKMIENRLD HHHHHHHHHHHHCCC	38.30	25381059
486	Phosphorylation	IENRLDGSKTPVGVH HHHCCCCCCCCCCCC	32.78	22369663
488	Phosphorylation	NRLDGSKTPVGVHTG HCCCCCCCCCCCCCC	25.55	22369663
494	Phosphorylation	KTPVGVHTGSALQRV CCCCCCCCCHHHHHH	30.35	22369663
496	Phosphorylation	PVGVHTGSALQRVRS CCCCCCCHHHHHHHC	27.28	22369663
503	Phosphorylation	SALQRVRSSNTLKSN HHHHHHHCCCCCCCC	25.69	19823750
504	Phosphorylation	ALQRVRSSNTLKSNR HHHHHHCCCCCCCCC	24.22	20377248
506	Phosphorylation	QRVRSSNTLKSNRSM HHHHCCCCCCCCCCC	37.19	20377248
509	Phosphorylation	RSSNTLKSNRSMQSG HCCCCCCCCCCCCCC	40.36	22369663
512	Phosphorylation	NTLKSNRSMQSGSGV CCCCCCCCCCCCCCC	25.98	22369663
515	Phosphorylation	KSNRSMQSGSGVASP CCCCCCCCCCCCCCC	26.70	22369663
517	Phosphorylation	NRSMQSGSGVASPID CCCCCCCCCCCCCCC	35.03	22369663
521	Phosphorylation	QSGSGVASPIDKVPN CCCCCCCCCCCCCCC	21.71	20377248
587	Ubiquitination	KAQYGPYKEKLDMYE HHHHCCHHHHHHHHH	52.05	23749301
634	Acetylation	NTSTIFGKLKDLTYK CCHHHHHHHHHCCCC	41.43	24489116
652	Ubiquitination	RLVDMVSKQGDVNNV HHHHHHHCCCCCCCE	47.06	23749301
663	Phosphorylation	VNNVWVQSVKELEME CCCEEHHHHHHHHHH	26.07	21551504
699	Phosphorylation	LHKKLLESHATAGNK HHHHHHHHHCCCCCH	20.56	21440633
702	Phosphorylation	KLLESHATAGNKESL HHHHHHCCCCCHHHH	29.67	21440633
708	Phosphorylation	ATAGNKESLENDKEQ CCCCCHHHHHCCCHH	42.46	20377248
717	Phosphorylation	ENDKEQESDTTASTL HCCCHHCCCCHHHHH	39.69	22369663
719	Phosphorylation	DKEQESDTTASTLGQ CCHHCCCCHHHHHHH	33.28	20377248
720	Phosphorylation	KEQESDTTASTLGQI CHHCCCCHHHHHHHH	24.49	22369663
722	Phosphorylation	QESDTTASTLGQIAK HCCCCHHHHHHHHHH	23.28	25521595
723	Phosphorylation	ESDTTASTLGQIAKF CCCCHHHHHHHHHHH	32.23	25521595
732	Phosphorylation	GQIAKFISATKEEDE HHHHHHHHCCCCCCC	32.77	23749301
734	Phosphorylation	IAKFISATKEEDEAS HHHHHHCCCCCCCCC	32.00	21551504
741	Phosphorylation	TKEEDEASDADEFYD CCCCCCCCCHHHHHH	31.14	22369663
747	Phosphorylation	ASDADEFYDAAELVD CCCHHHHHHHHHHHH	11.56	22369663
757	Phosphorylation	AELVDEVTELTEAHP HHHHHHHHHHHHHCC	24.19	22369663
760	Phosphorylation	VDEVTELTEAHPEIS HHHHHHHHHHCCCCC	25.54	22369663
767	Phosphorylation	TEAHPEISTAAAPKH HHHCCCCCCCCCCCC	15.44	22369663
768	Phosphorylation	EAHPEISTAAAPKHA HHCCCCCCCCCCCCC	26.44	22369663
783	Phosphorylation	PPPVPNETDNDSQYV CCCCCCCCCCCCHHC	46.90	21551504
787	Phosphorylation	PNETDNDSQYVQDEK CCCCCCCCHHCHHHH	29.66	22369663
789	Phosphorylation	ETDNDSQYVQDEKSK CCCCCCHHCHHHHHH	12.20	22369663
795	Phosphorylation	QYVQDEKSKIESNVE HHCHHHHHHHHHHHH	36.50	21440633
799	Phosphorylation	DEKSKIESNVEKTSQ HHHHHHHHHHHHHHH	50.04	26447709
804	Phosphorylation	IESNVEKTSQKFEKQ HHHHHHHHHHHHHHH	23.92	21440633
805	Phosphorylation	ESNVEKTSQKFEKQN HHHHHHHHHHHHHHC	42.30	20377248
816	Phosphorylation	EKQNNLVTEDEPKTD HHHCCCCCCCCCCCH	40.67	22369663
821	Ubiquitination	LVTEDEPKTDQSLKN CCCCCCCCCHHHHHC	63.79	23749301
822	Phosphorylation	VTEDEPKTDQSLKNF CCCCCCCCHHHHHCC	50.41	22369663
825	Phosphorylation	DEPKTDQSLKNFKAE CCCCCHHHHHCCCCC	44.09	22369663
827	Ubiquitination	PKTDQSLKNFKAEDK CCCHHHHHCCCCCCH	66.63	23749301
834	Ubiquitination	KNFKAEDKESQVKEK HCCCCCCHHHHHHHH	51.26	23749301
836	Phosphorylation	FKAEDKESQVKEKTK CCCCCHHHHHHHHHH	46.12	28889911
847	Phosphorylation	EKTKEIASSVIGEKT HHHHHHHHHHHCCCE	30.69	28152593
848	Phosphorylation	KTKEIASSVIGEKTI HHHHHHHHHHCCCEE	14.56	20377248
952	Phosphorylation	FEDSTLRTLYVAAFT CCHHHHHHHHHHHHH	26.08	28889911
954	Phosphorylation	DSTLRTLYVAAFTAS HHHHHHHHHHHHHHH	6.34	28889911
959	Phosphorylation	TLYVAAFTASSYAST HHHHHHHHHHHCHHC	22.39	28889911
967	Phosphorylation	ASSYASTTKRVAKPF HHHCHHCCCCCCCCC	17.85	28889911
1050	Acetylation	LRPNDNEKEELYTWK ECCCCCCCCCCEEEE	63.84	24489116
1142	Phosphorylation	VVKDGDLSLEKTRTA EEECCCCCCEEEECH	38.94	28889911
1146	Phosphorylation	GDLSLEKTRTAASAG CCCCCEEEECHHHCC	24.71	22369663
1148	Phosphorylation	LSLEKTRTAASAGNG CCCEEEECHHHCCCC	31.63	25521595
1151	Phosphorylation	EKTRTAASAGNGPTD EEEECHHHCCCCCCC	33.96	22369663
1157	Phosphorylation	ASAGNGPTDDGTKFL HHCCCCCCCCCCEEE	48.39	22890988
1161	Phosphorylation	NGPTDDGTKFLIWKA CCCCCCCCEEEEEEC	26.14	22890988

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of OSH2_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of OSH2_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of OSH2_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ATC3_YEAST	DRS2	physical	16554755
OSH1_YEAST	SWH1	physical	16554755
H2A2_YEAST	HTA2	physical	16554755
EIF3A_YEAST	RPG1	physical	16554755
YRA1_YEAST	YRA1	physical	16554755
COPB2_YEAST	SEC27	physical	16554755
LAA1_YEAST	LAA1	physical	16554755
SSO2_YEAST	SSO2	genetic	16269340
TSC3_YEAST	TSC3	genetic	16269340
PLSC_YEAST	SLC1	genetic	16269340
PST2_YEAST	PST2	genetic	16269340
MYO5_YEAST	MYO5	physical	18467557
UME1_YEAST	UME1	physical	18467557
HSP71_YEAST	SSA1	physical	19536198
OSH3_YEAST	OSH3	genetic	11238399
SYSC_YEAST	SES1	genetic	20526336
TOR2_YEAST	TOR2	genetic	20526336
KES1_YEAST	KES1	genetic	21819498
OSH2_YEAST	OSH2	physical	22940862
HSP71_YEAST	SSA1	physical	22940862
SCS2_YEAST	SCS2	physical	24366873
SCS22_YEAST	SCS22	physical	24366873
SEC22_YEAST	SEC22	physical	24705552
SSO1_YEAST	SSO1	physical	24705552
GPI19_YEAST	GPI19	genetic	27708008
COG2_YEAST	COG2	genetic	27708008
UTP9_YEAST	UTP9	genetic	27708008
KTHY_YEAST	CDC8	genetic	27708008
CDC11_YEAST	CDC11	genetic	27708008
SEC23_YEAST	SEC23	genetic	27708008

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"A multidimensional chromatography technology for in-depthphosphoproteome analysis.";
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
Mol. Cell. Proteomics 7:1389-1396(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-422; SER-445; THR-488;SER-515; SER-722; SER-732; SER-787; SER-825; THR-1146; THR-1148 ANDSER-1151, AND MASS SPECTROMETRY.
PubMed: 18407956

"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases.";
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7; THR-783; SER-787;SER-825; THR-1146; THR-1148 AND SER-1151, AND MASS SPECTROMETRY.
PubMed: 17563356

"Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry.";
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-422 AND SER-503, ANDMASS SPECTROMETRY.
PubMed: 17287358