dbPTM

PEP3_YEAST - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PEP3_YEAST
UniProt AC	P27801
Protein Name	Vacuolar membrane protein PEP3
Gene Name	PEP3
Organism	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length	918
Subcellular Localization	Vacuole membrane Peripheral membrane protein Cytoplasmic side.
Protein Description	Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion..
Protein Sequence	MIKTRIEEVQLQFLTGNTELTHLKVSNDQLIVTTQRTIYRINLQDPAIVNHFDCPLSKELETIMNVHVSPMGSVILIRTNFGRYMLLKDGEFTQLNKIKNLDLSSLHWINETTFLMGIKKTPKLYRVELTGKDITTKLWYENKKLSGGIDGIAYWEGSLLLTIKDNILYWRDVTNMKFPLVLPDESEQFERLKHHAIKKFDSYNGLFAWVTSNGIVFGDLKEKQMEKDPASNNFGKFLSSSKVLLNFELPDYQNDKDHLIKDIVLTAFHILLLRKNTVTMVSQLNNDVVFHETIPRHQLTGSNTDSNEKFLGLVRDSVKETFWCFSNINVFEIIIENEPNSVWNLLVRDNKFDKALSLKGLTVREIESVKLSKAMYLFHTAKDFHSAAQTLGSMKDLSHFGEIALNFLQIKDYNDLNVILIKQLDNVPWKSTQVVLSSWIIWNFMKQLNDIELKINTTKPASTDEDNLLNWNLNLKEKSNELTKFLESHLEKLDNETVYQIMSKQNRQNELLIFASLINDMKFLLSFWIDQGNWYESLKILLTINNHDLVYKYSLILLLNSPEATVSTWMKIKDLDPNKLIPTILKFFTNWQNNSKLITNISEYPENYSLTYLKWCVREVPKMCNPIVYNSILYMMITDPRNDMILENDIIKFMKSNENKYDLNFQLRLSLKFKKTKTSIFLLTRLNLFEDAIDLALKNNLIDDCKVIVNDEILIEDYKLRKRLWLKIAKHLLLSMKDIDIKQLIRTILNDSNEILTIKDLLPFFNEYTTIANLKEELIKFLENHNMKMNEISEDIINSKNLKVEINTEISKFNEIYRILEPGKSCDECGKFLQIKKFIVFPCGHCFHWNCIIRVILNSNDYNLRQKTENFLKAKSKHNLNDLENIIVEKCGLCSDININKIDQPISIDETELAKWNE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
236	Ubiquitination	PASNNFGKFLSSSKV CCCCCHHHHHCCCCE	38.71	24961812
300	Phosphorylation	TIPRHQLTGSNTDSN CCCHHHCCCCCCCCC	31.81	28889911
309	Acetylation	SNTDSNEKFLGLVRD CCCCCCHHHHHHHHH	50.57	24489116
368	Phosphorylation	LTVREIESVKLSKAM CCCEEEEEEEHHHHH	30.16	30377154
373	Acetylation	IESVKLSKAMYLFHT EEEEEHHHHHHHHHC	47.83	25381059
631	Phosphorylation	CNPIVYNSILYMMIT CCHHHCCCHHHHHCC	9.04	27017623
634	Phosphorylation	IVYNSILYMMITDPR HHCCCHHHHHCCCCC	5.29	27017623
638	Phosphorylation	SILYMMITDPRNDMI CHHHHHCCCCCCCEE	23.27	27017623
907	Phosphorylation	NKIDQPISIDETELA CCCCCCCCCCHHHHH	30.58	22369663
911	Phosphorylation	QPISIDETELAKWNE CCCCCCHHHHHHCCC	32.33	22369663
915	Ubiquitination	IDETELAKWNE---- CCHHHHHHCCC----	64.28	23749301

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of PEP3_YEAST !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of PEP3_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PEP3_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AAP1_YEAST	AAP1	physical	11805826
PEP5_YEAST	PEP5	physical	11805826
VPS16_YEAST	VPS16	physical	11805826
VPS33_YEAST	VPS33	physical	11805826
VPS41_YEAST	VPS41	physical	11805826
VPS8_YEAST	VPS8	physical	11805826
SEC7_YEAST	SEC7	physical	11805837
PP2C1_YEAST	PTC1	physical	11805837
VPS41_YEAST	VPS41	physical	10944212
VAM6_YEAST	VAM6	physical	10944212
PEP5_YEAST	PEP5	physical	10944212
VPS16_YEAST	VPS16	physical	10944212
VAM3_YEAST	VAM3	physical	11030345
VPS33_YEAST	VPS33	physical	10944212
PEP3_YEAST	PEP3	physical	10978279
PEP7_YEAST	PEP7	physical	10978279
VPS33_YEAST	VPS33	physical	9362071
VAM3_YEAST	VAM3	genetic	9362071
VPS16_YEAST	VPS16	genetic	9362071
PEP7_YEAST	PEP7	genetic	10978279
PEP7_YEAST	PEP7	genetic	11422941
VPS21_YEAST	VPS21	genetic	11422941
VPS45_YEAST	VPS45	genetic	11422941
AAP1_YEAST	AAP1	physical	16429126
PEP5_YEAST	PEP5	physical	16429126
VPS16_YEAST	VPS16	physical	16429126
VPS33_YEAST	VPS33	physical	16429126
VPS41_YEAST	VPS41	physical	16429126
VPS8_YEAST	VPS8	physical	16429126
PEP12_YEAST	PEP12	genetic	10978279
ARL1_YEAST	ARL1	genetic	20093466
HOSM_YEAST	LYS21	genetic	20093466
ISU1_YEAST	ISU1	genetic	20093466
VPS8_YEAST	VPS8	physical	21325627
PEP5_YEAST	PEP5	physical	21325627
VPS33_YEAST	VPS33	physical	21325627
YPT52_YEAST	YPT52	physical	21325627
PMA2_YEAST	PMA2	genetic	20526336
PEP5_YEAST	PEP5	physical	20604902
VAM6_YEAST	VAM6	physical	20604902
SCH9_YEAST	SCH9	genetic	24514902
TOR1_YEAST	TOR1	genetic	24514902
GTR1_YEAST	GTR1	genetic	24514902
GTR2_YEAST	GTR2	genetic	24514902
VATA_YEAST	VMA1	genetic	24514902
VPH2_YEAST	VPH2	genetic	24514902
PEP3_YEAST	PEP3	physical	25324549
PEP5_YEAST	PEP5	physical	25324549
VAM6_YEAST	VAM6	physical	25324549
VPS16_YEAST	VPS16	physical	25324549
VPS33_YEAST	VPS33	physical	25324549
VPS41_YEAST	VPS41	physical	25324549

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PEP3_YEAST

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-907, AND MASSSPECTROMETRY.	18407956 [Abstract]