| UniProt ID | TOM1_YEAST | |
|---|---|---|
| UniProt AC | Q03280 | |
| Protein Name | E3 ubiquitin-protein ligase TOM1 | |
| Gene Name | TOM1 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 3268 | |
| Subcellular Localization | Nucleus, nucleolus . | |
| Protein Description | Probable ubiquitin ligase protein involved in many cellular processes, such as transcription regulation, maintenance of nuclear structure, cell cycle, mRNA export and rRNA maturation. E3 ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. Involved in transcription regulation by interacting, and probably mediating, ubiquitination of some subunit of the SAGA complex. Required for SPT7 ubiquitination. Participates in mRNA export from the nucleus by regulating the transport of hnRNP proteins. Required for the shuttling of hnRNP protein NAB2, probably by mediating ubiquitination of a protein associated with NAB2. Also required for full induction of the general stress and heat-shock responses. Involved in 18S rRNA maturation by affecting several early steps in the rRNA processing pathway.. | |
| Protein Sequence | MVLFTRCEKARKEKLAAGYKPLVDYLIDCDTPTFLERIEAIQEWDRSRDDLYVWIPILDRMDGLLLKVAEKYKYKQDPKKECEVKLVEMEAHDVDYCLKMLKFTRRLLLNTENRFVYSSGDVLMYLLNCPNFTIKLAVMRILAILGERFVIAREKIVAHNIFGDHNLRKKTLKLALSLSSSVMDEDGEHFSLVDLYFDKKKVPQKWRKLRFTHYTSNDFKKSSQQKNNINETQTSIKKVTMTTQELCEHSLQQIFDKGMALLPAESWFDFSIKASVAKAFSDDSGENIDLRNIIIETKLNAIAFVNTIFSPPQVSSKLFELDPYAFNSLTDLISLSETKIPKELRTDALFTLECISLKHVWCSDIIRNLGGNISHGLLFQILRYIAKTLREATDEIDEEYNVRFFYLISNLADVKPLHESLFAAGLIPTLLEIVSIRNCPYKRTLASATHLLETFIDNSETTTEFIENDGFTMLITSVANEIDFTLAHPETWQPPKYSVVYYSISFRELAYIRSLLKLVLKLLSTDSGDRIRNLIDSPILVSLKKILENKLVFGLTLITYTLDVVQKVINSEPTIYPVLVEAGLIPYVIDNFPKLIGPSAELLSLLPDVVSAICLNPEGLKQVKEKGLINNLFDFLLDADHARILTGGDRSTEYGTDIDELARHYPDLKANIVEALCNVIRKMPSTFRNEREFLFTSPKDQKYFFHRKNEEILTDKEEHEPAYWELLDKGTMLDTFTSVLFGMSLGNGSFSQVPQHLEARDFLAIIFMENPPYEYFTSVAISNVTEVLQYLDEKYEDYAFMDVMKVLNDQLENLNDFLNSPNDRSFFLERDGENSVRSCHSKLCRLAAILNIVTNVYIDLTTLSCKRIMQIYSYFDKRGFSLIKNLKLLFQKCALEEMYIRQHMPDSVITETMPLPIVDVSGDGPPLQIYIDDPKKGDQKGKITSVKTRNTLQMRTILYTLQSNTAILFRCFLRLSHSRNMDLEHKDLTTEVHIFENVVENVIEMLKATELEGHLPYILVLLNFNTFVFTIPKASPNSTEILQTIPAYIFYQKGGYLLYLHIIRDLFTRMTKIKDLSSLDNINYIDESNGILTLSCLINALTFYNKSMQTETMENVQSIGKYYVSIDDDYNIMKALTVPIKVMALAMILDLDKSDSLFKTQSRNVPYSVFKQLLSMLKNIFTNVNIYTKELYELHWDLIFPPIKKISLFEQVGIPGDVAANYLTDTGDDLPADNSIGLFSPEQWEKYKKLIGEDKSIYYPQPMQAQYYKGCSSKELDELRDTFFNDGLPSRIFTVLPFYPKLVNAFAKTLLQIFTKYDEPTEVFAGRILDRILETDLDDPATLSSLIHLFGIFLNEKYIYQKASHLMQRFIEYLEKSLKPEHVNTPWFSKALYVYEIILAKSELPHLEELSKDVLLRYPLLSMAKVFRIPDPMKQKLFDILIRVSDISNFYSALATSRILIFYSRDELYANNIARSGILSRLLKVIGSFQKLDKINFLESSFLLLTRRCFETTENVDALIRAEINRSFTARPLGGGDDAVRELTTILEEKAHVVMRSPSQFIDVLCETARFHEFDDQGALVDYSLKRFLGEKDKNTQASSTEKSDIYERTGIMHLLLSQLMAASEKDWLSEPANSSDLPENKKAQLDPSRNPVCAYMIFLLKLLVELVSSYNQCKFEFLTFSRRNTYAERPRPRTTAINFFLYRLLDKPVGTDHDKHEAKRREVIGMLARSVIIGFLATVQDDRTTKTDVKLADPHMNFIRKFAIEAIIKAIRNATSSSKLLESNHLKLDMWFRIITSMVYVQAPYLRQLLDSNKVEADQYQLCKLVIDLGLPSVITEAMASIDLNYPFSKKIFNVAVEALNTISSTRNNFSEHFKIEDHDEVEDEVDESDKEEIPDMFKNSALGMYDVEDIEEDDDDDTSLIGDDDAMAFVDSDNGFEVVFSDEDDDMGEEDADDARSDSEENELSSEMQSSTADGTDVDYEVDDADGLIINIDQPSGDDEEMADYDANISHSSHSENEDDASMDVIEVYDDELSSGYDVDLSDYDVDESDWDSGLSSLSISDEDSESSEDEPINSTRMGDSRRRWLIAEGVELTDDSQGESEEDDRGVFRGIEHIFSNENEPLFRVHDEMRHRNHHRSINRTHFHSAMSAPSLSLLNRGRRNQSNLINPLGPTGLEQVENDISDQVTVAGSGSRPRSHHLHFSEVLVSGSFFDEPVLDGIILKSTVSRWKDIFDMFYDSKTYANCIIPTVINRLYKVSLALQKDLENKREQEKLKNKNLLFNEAKVESHNSSDAISVEQDDIQESNVTHDDHEPVYVTIQGSEVDIGGTDIDPEFMNALPDDIRADVFAQHVRERRAEARLNSDHNVHSREIDSDFLEAIPEDIREGILDTEAEEQRMFGRIGSSADVIRADDDVSNNDEEVENGLDHGNSNDRNNADPEKKKPARIYFAPLIDRAGIASLMKSVFISKPYIQREIYHELFYRLCSSKQNRNDLMNTFLFILSEGIIDQHSLEKVYNIISSRAMGHAKTTTVRQLPSDCTPLTVANQTIEILQSLIDADSRLKYFLIAEHDNLIVNKANNKSRKEALPDKKLRWPLWHLFSLLDRKLITDESVLMDLLTRILQVCTKTLAVLSTSSNGKENLSKKFHLPSFDEDDLMKILSIIMLDSCTTRVFQQTLNIIYNLSKLQGCMSIFTKHLVSLAISIMSKLKSALDGLSREVGTITTGMEINSELLQKFTLPSSDQAKLLKILTTVDFLYTHKRKEEERNVKDLQSLYDKMNGGPVWSSLSECLSQFEKSQAINTSATILLPLIESLMVVCRRSDLSQNRNTAVKYEDAKLLDFSKTRVENLFFPFTDAHKKLLNQMIRSNPKLMSGPFALLVKNPKVLDFDNKRYFFNAKLKSDNQERPKLPITVRREQVFLDSYRALFFKTNDEIKNSKLEITFKGESGVDAGGVTREWYQVLSRQMFNPDYALFLPVPSDKTTFHPNRTSGINPEHLSFFKFIGMIIGKAIRDQCFLDCHFSREVYKNILGRPVSLKDMESLDPDYYKSLVWILENDITDIIEETFSVETDDYGEHKVINLIEGGKDIIVTEANKQDYVKKVVEYKLQTSVKEQMDNFLVGFYALISKDLITIFDEQELELLISGLPDIDVDDWKNNTTYVNYTATCKEVSYFWRAVRSFDAEERAKLLQFVTGTSKVPLNGFKELSGVNGVCKFSIHRDFGSSERLPSSHTCFNQLNLPPYESYETLRGSLLLAINEGHEGFGLA | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 155 | Ubiquitination | RFVIAREKIVAHNIF CEEEECHHHHHHHCC | 36.61 | 17644757 | |
| 169 | Ubiquitination | FGDHNLRKKTLKLAL CCCCCHHHHHHHHHH | 53.96 | 17644757 | |
| 220 | Ubiquitination | HYTSNDFKKSSQQKN ECCCCCHHHHHHHCC | 55.39 | 17644757 | |
| 221 | Ubiquitination | YTSNDFKKSSQQKNN CCCCCHHHHHHHCCC | 56.16 | 17644757 | |
| 226 | Ubiquitination | FKKSSQQKNNINETQ HHHHHHHCCCCCHHH | 44.30 | 23749301 | |
| 234 | Phosphorylation | NNINETQTSIKKVTM CCCCHHHHHHHHHHH | 39.58 | 29136822 | |
| 235 | Phosphorylation | NINETQTSIKKVTMT CCCHHHHHHHHHHHC | 23.69 | 30377154 | |
| 278 | Ubiquitination | SIKASVAKAFSDDSG EEHHHHHHHHCCCCC | 48.05 | 23749301 | |
| 298 | Ubiquitination | RNIIIETKLNAIAFV HHEEHHHHHHHEEEE | 27.27 | 17644757 | |
| 317 | Ubiquitination | SPPQVSSKLFELDPY CCCCHHHHHHCCCHH | 50.09 | 17644757 | |
| 420 | Phosphorylation | DVKPLHESLFAAGLI CCHHHHHHHHHCCCH | 20.31 | 26447709 | |
| 429 | Phosphorylation | FAAGLIPTLLEIVSI HHCCCHHHHHHHHHH | 36.66 | 26447709 | |
| 435 | Phosphorylation | PTLLEIVSIRNCPYK HHHHHHHHHCCCCCH | 22.84 | 26447709 | |
| 669 | Ubiquitination | ARHYPDLKANIVEAL HHHCCHHHHHHHHHH | 46.93 | 17644757 | |
| 682 | Ubiquitination | ALCNVIRKMPSTFRN HHHHHHHHCCCCCCC | 44.00 | 17644757 | |
| 872 | Phosphorylation | CKRIMQIYSYFDKRG HHHHHHHHHHHHHCC | 4.76 | 27017623 | |
| 873 | Phosphorylation | KRIMQIYSYFDKRGF HHHHHHHHHHHHCCC | 22.43 | 27017623 | |
| 874 | Phosphorylation | RIMQIYSYFDKRGFS HHHHHHHHHHHCCCH | 9.99 | 27017623 | |
| 1256 | Phosphorylation | KLIGEDKSIYYPQPM HHHCCCCCCCCCCCC | 28.30 | 21551504 | |
| 1258 | Phosphorylation | IGEDKSIYYPQPMQA HCCCCCCCCCCCCCC | 19.04 | 21551504 | |
| 1259 | Phosphorylation | GEDKSIYYPQPMQAQ CCCCCCCCCCCCCCH | 8.17 | 21551504 | |
| 1308 | Ubiquitination | KLVNAFAKTLLQIFT HHHHHHHHHHHHHHH | 33.00 | 17644757 | |
| 1316 | Ubiquitination | TLLQIFTKYDEPTEV HHHHHHHCCCCCCHH | 39.70 | 17644757 | |
| 1335 | Phosphorylation | ILDRILETDLDDPAT HHHHHHHCCCCCHHH | 38.12 | 19779198 | |
| 1342 | Phosphorylation | TDLDDPATLSSLIHL CCCCCHHHHHHHHHH | 32.34 | 19779198 | |
| 1357 | Ubiquitination | FGIFLNEKYIYQKAS HHHHHCHHHHHHHHH | 35.56 | 17644757 | |
| 1393 | Phosphorylation | PWFSKALYVYEIILA CHHHHHHHHHHHHHH | 13.07 | 22369663 | |
| 1395 | Phosphorylation | FSKALYVYEIILAKS HHHHHHHHHHHHHHC | 6.45 | 22369663 | |
| 1412 | Acetylation | PHLEELSKDVLLRYP CCHHHHCHHHHHHHH | 65.56 | 24489116 | |
| 1557 | Phosphorylation | KAHVVMRSPSQFIDV HCCEEECCHHHHHHH | 15.71 | 27214570 | |
| 1559 | Phosphorylation | HVVMRSPSQFIDVLC CEEECCHHHHHHHHH | 38.68 | 29734811 | |
| 1592 | Acetylation | LKRFLGEKDKNTQAS HHHHHCCCCCCCCCC | 72.68 | 25381059 | |
| 1594 | Ubiquitination | RFLGEKDKNTQASST HHHCCCCCCCCCCCC | 73.32 | 17644757 | |
| 1603 | Ubiquitination | TQASSTEKSDIYERT CCCCCCCHHHHHHHH | 54.22 | 17644757 | |
| 1618 | Phosphorylation | GIMHLLLSQLMAASE CHHHHHHHHHHHHCC | 22.65 | 27017623 | |
| 1708 | Ubiquitination | FLYRLLDKPVGTDHD HHHHHHCCCCCCCCC | 41.39 | 17644757 | |
| 1716 | Ubiquitination | PVGTDHDKHEAKRRE CCCCCCCHHHHHHHH | 39.67 | 17644757 | |
| 1720 | Ubiquitination | DHDKHEAKRREVIGM CCCHHHHHHHHHHHH | 49.34 | 17644757 | |
| 1851 | Ubiquitination | DLNYPFSKKIFNVAV CCCCCCCHHHHHHHH | 50.71 | 17644757 | |
| 1852 | Ubiquitination | LNYPFSKKIFNVAVE CCCCCCHHHHHHHHH | 51.86 | 17644757 | |
| 1890 | Phosphorylation | VEDEVDESDKEEIPD HCCCCCCCHHHHCCH | 49.90 | 25521595 | |
| 2096 | Phosphorylation | IAEGVELTDDSQGES EEECCEECCCCCCCC | 25.01 | 19795423 | |
| 2099 | Phosphorylation | GVELTDDSQGESEED CCEECCCCCCCCCCC | 42.78 | 19795423 | |
| 2103 | Phosphorylation | TDDSQGESEEDDRGV CCCCCCCCCCCCCHH | 53.78 | 19795423 | |
| 2119 | Phosphorylation | RGIEHIFSNENEPLF CCHHHHHCCCCCCCE | 42.50 | 22369663 | |
| 2144 | Phosphorylation | HHRSINRTHFHSAMS CCCCCCHHHHHCCCC | 25.14 | 30377154 | |
| 2151 | Phosphorylation | THFHSAMSAPSLSLL HHHHCCCCCCCHHHH | 36.42 | 21440633 | |
| 2154 | Phosphorylation | HSAMSAPSLSLLNRG HCCCCCCCHHHHHCC | 30.24 | 30377154 | |
| 2166 | Phosphorylation | NRGRRNQSNLINPLG HCCCCCCCCCCCCCC | 36.83 | 30377154 | |
| 2175 | Phosphorylation | LINPLGPTGLEQVEN CCCCCCCCCHHHHHC | 53.23 | 27017623 | |
| 2185 | Phosphorylation | EQVENDISDQVTVAG HHHHCCCCCCEEECC | 25.50 | 22369663 | |
| 2189 | Phosphorylation | NDISDQVTVAGSGSR CCCCCCEEECCCCCC | 10.06 | 28889911 | |
| 2242 | Ubiquitination | FDMFYDSKTYANCII HHHHHCCCCHHCCHH | 42.53 | 17644757 | |
| 2279 | Ubiquitination | EQEKLKNKNLLFNEA HHHHHCCCCCCCCHH | 47.59 | 23749301 | |
| 2365 | Phosphorylation | RAEARLNSDHNVHSR HHHHHHCCCCCCCHH | 44.85 | 30377154 | |
| 2376 | Phosphorylation | VHSREIDSDFLEAIP CCHHHCCHHHHHHCC | 35.60 | 19547744 | |
| 2406 | Phosphorylation | RMFGRIGSSADVIRA HHHHCCCCCCCEEEC | 21.71 | 22369663 | |
| 2407 | Phosphorylation | MFGRIGSSADVIRAD HHHCCCCCCCEEECC | 24.23 | 22369663 | |
| 2418 | Phosphorylation | IRADDDVSNNDEEVE EECCCCCCCCHHHHH | 36.59 | 17330950 | |
| 2433 | Phosphorylation | NGLDHGNSNDRNNAD HCCCCCCCCCCCCCC | 44.70 | 29136822 | |
| 2565 | Ubiquitination | IDADSRLKYFLIAEH CCCCCCCCEEEEEEC | 32.61 | 17644757 | |
| 2579 | Ubiquitination | HDNLIVNKANNKSRK CCCEEEECCCCCCHH | 41.41 | 17644757 | |
| 2614 | Phosphorylation | RKLITDESVLMDLLT HHCCCCHHHHHHHHH | 24.30 | 28889911 | |
| 2621 | Phosphorylation | SVLMDLLTRILQVCT HHHHHHHHHHHHHHH | 23.98 | 28889911 | |
| 2663 | Phosphorylation | DDLMKILSIIMLDSC HHHHHHHHHHHHHCC | 17.34 | 28889911 | |
| 2671 | Phosphorylation | IIMLDSCTTRVFQQT HHHHHCCCHHHHHHH | 22.91 | 28889911 | |
| 2708 | Phosphorylation | SLAISIMSKLKSALD HHHHHHHHHHHHHHH | 32.92 | 27017623 | |
| 2750 | Ubiquitination | SDQAKLLKILTTVDF HHHHHHHHHHHHHHH | 46.21 | 17644757 | |
| 2762 | Ubiquitination | VDFLYTHKRKEEERN HHHHHHCCCHHHHCC | 58.32 | 17644757 | |
| 2771 | Acetylation | KEEERNVKDLQSLYD HHHHCCHHHHHHHHH | 57.16 | 24489116 | |
| 2839 | Acetylation | AVKYEDAKLLDFSKT CCCHHHHHHHCCCCC | 63.00 | 24489116 | |
| 2845 | Ubiquitination | AKLLDFSKTRVENLF HHHHCCCCCHHHHCC | 40.10 | 17644757 | |
| 2845 | Acetylation | AKLLDFSKTRVENLF HHHHCCCCCHHHHCC | 40.10 | 24489116 | |
| 2860 | Ubiquitination | FPFTDAHKKLLNQMI CCCCHHHHHHHHHHH | 47.00 | 17644757 | |
| 2861 | Ubiquitination | PFTDAHKKLLNQMIR CCCHHHHHHHHHHHH | 48.62 | 17644757 | |
| 2875 | Phosphorylation | RSNPKLMSGPFALLV HHCCCHHCCCEEEEE | 54.47 | 19795423 | |
| 2983 | Ubiquitination | FLPVPSDKTTFHPNR EEECCCCCCCCCCCC | 54.52 | 17644757 | |
| 3003 | Ubiquitination | PEHLSFFKFIGMIIG HHHHHHHHHHHHHHH | 33.78 | 17644757 | |
| 3079 | Ubiquitination | TDDYGEHKVINLIEG CCCCCCEEEEEEEEC | 40.27 | 17644757 | |
| 3088 | Ubiquitination | INLIEGGKDIIVTEA EEEEECCEEEEEEEC | 57.52 | 17644757 | |
| 3097 | Ubiquitination | IIVTEANKQDYVKKV EEEEECCHHHHHHHH | 52.15 | 17644757 | |
| 3102 | Ubiquitination | ANKQDYVKKVVEYKL CCHHHHHHHHHHHHH | 33.35 | 17644757 | |
| 3103 | Ubiquitination | NKQDYVKKVVEYKLQ CHHHHHHHHHHHHHH | 41.28 | 17644757 | |
| 3189 | Ubiquitination | FDAEERAKLLQFVTG CCHHHHHHHHHHHHC | 56.82 | 17644757 | |
| 3199 | Ubiquitination | QFVTGTSKVPLNGFK HHHHCCCCCCCCCCH | 47.94 | 17644757 | |
| 3206 | Ubiquitination | KVPLNGFKELSGVNG CCCCCCCHHHCCCCC | 60.18 | 17644757 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of TOM1_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of TOM1_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of TOM1_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1557; SER-1890;SER-2119; SER-2151; SER-2185; THR-2189; SER-2376; SER-2406; SER-2407AND SER-2418, AND MASS SPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1890; SER-2119; SER-2185AND SER-2418, AND MASS SPECTROMETRY. | |
| "Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae."; Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.; J. Proteome Res. 6:1190-1197(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1890 AND SER-2418, ANDMASS SPECTROMETRY. | |
