dbPTM

PGK_YEAST - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PGK_YEAST
UniProt AC	P00560
Protein Name	Phosphoglycerate kinase
Gene Name	PGK1
Organism	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length	416
Subcellular Localization	Cytoplasm .
Protein Description
Protein Sequence	MSLSSKLSVQDLDLKDKRVFIRVDFNVPLDGKKITSNQRIVAALPTIKYVLEHHPRYVVLASHLGRPNGERNEKYSLAPVAKELQSLLGKDVTFLNDCVGPEVEAAVKASAPGSVILLENLRYHIEEEGSRKVDGQKVKASKEDVQKFRHELSSLADVYINDAFGTAHRAHSSMVGFDLPQRAAGFLLEKELKYFGKALENPTRPFLAILGGAKVADKIQLIDNLLDKVDSIIIGGGMAFTFKKVLENTEIGDSIFDKAGAEIVPKLMEKAKAKGVEVVLPVDFIIADAFSADANTKTVTDKEGIPAGWQGLDNGPESRKLFAATVAKAKTIVWNGPPGVFEFEKFAAGTKALLDEVVKSSAAGNTVIIGGGDTATVAKKYGVTDKISHVSTGGGASLELLEGKELPGVAFLSEKK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSLSSKLSV ------CCCCCCCCC	36.64	22814378
2	Phosphorylation	------MSLSSKLSV ------CCCCCCCCC	36.64	22369663
4	Phosphorylation	----MSLSSKLSVQD ----CCCCCCCCCCC	20.37	22369663
5	Phosphorylation	---MSLSSKLSVQDL ---CCCCCCCCCCCC	42.80	22369663
6	Acetylation	--MSLSSKLSVQDLD --CCCCCCCCCCCCC	41.21	22865919
6	Succinylation	--MSLSSKLSVQDLD --CCCCCCCCCCCCC	41.21	23954790
6	Ubiquitination	--MSLSSKLSVQDLD --CCCCCCCCCCCCC	41.21	24961812
8	Phosphorylation	MSLSSKLSVQDLDLK CCCCCCCCCCCCCCC	22.82	22369663
15	2-Hydroxyisobutyrylation	SVQDLDLKDKRVFIR CCCCCCCCCCEEEEE	61.95	-
15	Acetylation	SVQDLDLKDKRVFIR CCCCCCCCCCEEEEE	61.95	24489116
15	Succinylation	SVQDLDLKDKRVFIR CCCCCCCCCCEEEEE	61.95	23954790
15	Ubiquitination	SVQDLDLKDKRVFIR CCCCCCCCCCEEEEE	61.95	17644757
17	Acetylation	QDLDLKDKRVFIRVD CCCCCCCCEEEEEEE	49.20	24489116
17	Ubiquitination	QDLDLKDKRVFIRVD CCCCCCCCEEEEEEE	49.20	15699485
32	2-Hydroxyisobutyrylation	FNVPLDGKKITSNQR EECCCCCCCCCCCHH	40.44	-
32	Acetylation	FNVPLDGKKITSNQR EECCCCCCCCCCCHH	40.44	24489116
32	Succinylation	FNVPLDGKKITSNQR EECCCCCCCCCCCHH	40.44	23954790
32	Ubiquitination	FNVPLDGKKITSNQR EECCCCCCCCCCCHH	40.44	24961812
33	2-Hydroxyisobutyrylation	NVPLDGKKITSNQRI ECCCCCCCCCCCHHH	57.66	-
33	Ubiquitination	NVPLDGKKITSNQRI ECCCCCCCCCCCHHH	57.66	24961812
35	Phosphorylation	PLDGKKITSNQRIVA CCCCCCCCCCHHHHH	30.42	21440633
36	Phosphorylation	LDGKKITSNQRIVAA CCCCCCCCCHHHHHH	34.14	20377248
46	Phosphorylation	RIVAALPTIKYVLEH HHHHHHHHHHHHHHH	30.83	28152593
48	2-Hydroxyisobutyrylation	VAALPTIKYVLEHHP HHHHHHHHHHHHHCC	31.61	-
48	Acetylation	VAALPTIKYVLEHHP HHHHHHHHHHHHHCC	31.61	24489116
48	Succinylation	VAALPTIKYVLEHHP HHHHHHHHHHHHHCC	31.61	23954790
48	Ubiquitination	VAALPTIKYVLEHHP HHHHHHHHHHHHHCC	31.61	23749301
49	Phosphorylation	AALPTIKYVLEHHPR HHHHHHHHHHHHCCC	13.35	28889911
62	Phosphorylation	PRYVVLASHLGRPNG CCEEEEEHHCCCCCC	18.32	28889911
74	2-Hydroxyisobutyrylation	PNGERNEKYSLAPVA CCCCCCCCCCHHHHH	43.62	-
74	Acetylation	PNGERNEKYSLAPVA CCCCCCCCCCHHHHH	43.62	24489116
74	Ubiquitination	PNGERNEKYSLAPVA CCCCCCCCCCHHHHH	43.62	23749301
75	Phosphorylation	NGERNEKYSLAPVAK CCCCCCCCCHHHHHH	11.61	22369663
76	Phosphorylation	GERNEKYSLAPVAKE CCCCCCCCHHHHHHH	29.17	22369663
82	Acetylation	YSLAPVAKELQSLLG CCHHHHHHHHHHHHC	60.22	24489116
82	Succinylation	YSLAPVAKELQSLLG CCHHHHHHHHHHHHC	60.22	23954790
82	Ubiquitination	YSLAPVAKELQSLLG CCHHHHHHHHHHHHC	60.22	24961812
86	Phosphorylation	PVAKELQSLLGKDVT HHHHHHHHHHCCCCE	37.88	25521595
90	Acetylation	ELQSLLGKDVTFLND HHHHHHCCCCEECCC	49.33	25381059
90	Ubiquitination	ELQSLLGKDVTFLND HHHHHHCCCCEECCC	49.33	17644757
93	Phosphorylation	SLLGKDVTFLNDCVG HHHCCCCEECCCCCC	32.32	11283598
98	Glutathionylation	DVTFLNDCVGPEVEA CCEECCCCCCHHHHH	3.57	22833525
108	Ubiquitination	PEVEAAVKASAPGSV HHHHHHHHCCCCCEE	31.71	17644757
110	Phosphorylation	VEAAVKASAPGSVIL HHHHHHCCCCCEEEE	29.31	21082442
114	Phosphorylation	VKASAPGSVILLENL HHCCCCCEEEEEECH	12.62	22369663
123	Phosphorylation	ILLENLRYHIEEEGS EEEECHHEEHHHCCC	15.43	22369663
130	Phosphorylation	YHIEEEGSRKVDGQK EEHHHCCCCCCCCEE	32.01	22369663
132	Ubiquitination	IEEEGSRKVDGQKVK HHHCCCCCCCCEEEC	46.50	22817900
137	2-Hydroxyisobutyrylation	SRKVDGQKVKASKED CCCCCCEEECCCHHH	51.75	-
137	Acetylation	SRKVDGQKVKASKED CCCCCCEEECCCHHH	51.75	24489116
137	Ubiquitination	SRKVDGQKVKASKED CCCCCCEEECCCHHH	51.75	22817900
139	Ubiquitination	KVDGQKVKASKEDVQ CCCCEEECCCHHHHH	54.71	22817900
142	2-Hydroxyisobutyrylation	GQKVKASKEDVQKFR CEEECCCHHHHHHHH	63.89	-
142	Acetylation	GQKVKASKEDVQKFR CEEECCCHHHHHHHH	63.89	24489116
142	Succinylation	GQKVKASKEDVQKFR CEEECCCHHHHHHHH	63.89	23954790
142	Ubiquitination	GQKVKASKEDVQKFR CEEECCCHHHHHHHH	63.89	22817900
147	2-Hydroxyisobutyrylation	ASKEDVQKFRHELSS CCHHHHHHHHHHHHH	44.19	-
147	Acetylation	ASKEDVQKFRHELSS CCHHHHHHHHHHHHH	44.19	24489116
147	Succinylation	ASKEDVQKFRHELSS CCHHHHHHHHHHHHH	44.19	23954790
147	Ubiquitination	ASKEDVQKFRHELSS CCHHHHHHHHHHHHH	44.19	17644757
153	Phosphorylation	QKFRHELSSLADVYI HHHHHHHHHHHHHHH	21.96	19779198
154	Phosphorylation	KFRHELSSLADVYIN HHHHHHHHHHHHHHH	39.95	22369663
159	Phosphorylation	LSSLADVYINDAFGT HHHHHHHHHHHHCCC	8.44	21440633
172	Phosphorylation	GTAHRAHSSMVGFDL CCCHHHHHHCCCCCC	21.10	22369663
173	Phosphorylation	TAHRAHSSMVGFDLP CCHHHHHHCCCCCCC	13.97	22369663
190	Acetylation	AAGFLLEKELKYFGK HHHHHHHHHHHHHHH	69.21	24489116
190	Succinylation	AAGFLLEKELKYFGK HHHHHHHHHHHHHHH	69.21	23954790
190	Ubiquitination	AAGFLLEKELKYFGK HHHHHHHHHHHHHHH	69.21	23749301
193	2-Hydroxyisobutyrylation	FLLEKELKYFGKALE HHHHHHHHHHHHHHC	39.36	-
193	Acetylation	FLLEKELKYFGKALE HHHHHHHHHHHHHHC	39.36	24489116
193	Succinylation	FLLEKELKYFGKALE HHHHHHHHHHHHHHC	39.36	23954790
193	Ubiquitination	FLLEKELKYFGKALE HHHHHHHHHHHHHHC	39.36	23749301
197	Acetylation	KELKYFGKALENPTR HHHHHHHHHHCCCCC	39.64	24489116
197	Ubiquitination	KELKYFGKALENPTR HHHHHHHHHHCCCCC	39.64	23749301
203	Phosphorylation	GKALENPTRPFLAIL HHHHCCCCCCHHHHH	63.66	22369663
214	Acetylation	LAILGGAKVADKIQL HHHHCCCHHHHHHHH	41.03	24489116
214	Succinylation	LAILGGAKVADKIQL HHHHCCCHHHHHHHH	41.03	23954790
214	Ubiquitination	LAILGGAKVADKIQL HHHHCCCHHHHHHHH	41.03	23749301
218	Acetylation	GGAKVADKIQLIDNL CCCHHHHHHHHHHHH	23.71	24489116
218	Ubiquitination	GGAKVADKIQLIDNL CCCHHHHHHHHHHHH	23.71	24961812
241	Phosphorylation	IGGGMAFTFKKVLEN ECCCHHEEHHHHHHC	25.63	28889911
243	Ubiquitination	GGMAFTFKKVLENTE CCHHEEHHHHHHCCC	38.63	17644757
244	2-Hydroxyisobutyrylation	GMAFTFKKVLENTEI CHHEEHHHHHHCCCC	48.11	-
244	Ubiquitination	GMAFTFKKVLENTEI CHHEEHHHHHHCCCC	48.11	17644757
249	Phosphorylation	FKKVLENTEIGDSIF HHHHHHCCCCCCHHH	21.43	29136822
254	Phosphorylation	ENTEIGDSIFDKAGA HCCCCCCHHHHHHCC	21.68	29136822
258	Acetylation	IGDSIFDKAGAEIVP CCCHHHHHHCCCHHH	37.66	24489116
258	Succinylation	IGDSIFDKAGAEIVP CCCHHHHHHCCCHHH	37.66	23954790
258	Ubiquitination	IGDSIFDKAGAEIVP CCCHHHHHHCCCHHH	37.66	23749301
266	2-Hydroxyisobutyrylation	AGAEIVPKLMEKAKA HCCCHHHHHHHHHHH	49.96	-
266	Acetylation	AGAEIVPKLMEKAKA HCCCHHHHHHHHHHH	49.96	24489116
266	Succinylation	AGAEIVPKLMEKAKA HCCCHHHHHHHHHHH	49.96	23954790
266	Ubiquitination	AGAEIVPKLMEKAKA HCCCHHHHHHHHHHH	49.96	23749301
270	2-Hydroxyisobutyrylation	IVPKLMEKAKAKGVE HHHHHHHHHHHCCCE	41.02	-
274	Ubiquitination	LMEKAKAKGVEVVLP HHHHHHHCCCEEEEE	63.88	22106047
297	Ubiquitination	FSADANTKTVTDKEG CCCCCCCCCCCCCCC	40.58	22817900
298	Phosphorylation	SADANTKTVTDKEGI CCCCCCCCCCCCCCC	26.97	28889911
300	Phosphorylation	DANTKTVTDKEGIPA CCCCCCCCCCCCCCC	46.73	28132839
302	2-Hydroxyisobutyrylation	NTKTVTDKEGIPAGW CCCCCCCCCCCCCCC	48.77	-
302	Acetylation	NTKTVTDKEGIPAGW CCCCCCCCCCCCCCC	48.77	24489116
302	Succinylation	NTKTVTDKEGIPAGW CCCCCCCCCCCCCCC	48.77	23954790
302	Ubiquitination	NTKTVTDKEGIPAGW CCCCCCCCCCCCCCC	48.77	23749301
318	Phosphorylation	GLDNGPESRKLFAAT CCCCCHHHHHHHHHH	37.54	11283598
320	Acetylation	DNGPESRKLFAATVA CCCHHHHHHHHHHHH	58.18	22865919
320	Ubiquitination	DNGPESRKLFAATVA CCCHHHHHHHHHHHH	58.18	23749301
325	Phosphorylation	SRKLFAATVAKAKTI HHHHHHHHHHCCEEE	19.98	20377248
328	2-Hydroxyisobutyrylation	LFAATVAKAKTIVWN HHHHHHHCCEEEEEC	46.19	-
328	Succinylation	LFAATVAKAKTIVWN HHHHHHHCCEEEEEC	46.19	23954790
328	Ubiquitination	LFAATVAKAKTIVWN HHHHHHHCCEEEEEC	46.19	17644757
330	2-Hydroxyisobutyrylation	AATVAKAKTIVWNGP HHHHHCCEEEEECCC	37.77	-
330	Acetylation	AATVAKAKTIVWNGP HHHHHCCEEEEECCC	37.77	24489116
330	Ubiquitination	AATVAKAKTIVWNGP HHHHHCCEEEEECCC	37.77	17644757
331	Phosphorylation	ATVAKAKTIVWNGPP HHHHCCEEEEECCCC	26.14	22369663
345	2-Hydroxyisobutyrylation	PGVFEFEKFAAGTKA CCCEEHHHHHCHHHH	45.70	-
345	Acetylation	PGVFEFEKFAAGTKA CCCEEHHHHHCHHHH	45.70	24489116
345	Ubiquitination	PGVFEFEKFAAGTKA CCCEEHHHHHCHHHH	45.70	23749301
350	Phosphorylation	FEKFAAGTKALLDEV HHHHHCHHHHHHHHH	14.39	21440633
351	2-Hydroxyisobutyrylation	EKFAAGTKALLDEVV HHHHCHHHHHHHHHH	35.80	-
351	Acetylation	EKFAAGTKALLDEVV HHHHCHHHHHHHHHH	35.80	24489116
351	Ubiquitination	EKFAAGTKALLDEVV HHHHCHHHHHHHHHH	35.80	23749301
359	Acetylation	ALLDEVVKSSAAGNT HHHHHHHHHCCCCCE	43.82	24489116
359	Succinylation	ALLDEVVKSSAAGNT HHHHHHHHHCCCCCE	43.82	23954790
360	Phosphorylation	LLDEVVKSSAAGNTV HHHHHHHHCCCCCEE	17.38	22369663
361	Phosphorylation	LDEVVKSSAAGNTVI HHHHHHHCCCCCEEE	19.20	22369663
366	Phosphorylation	KSSAAGNTVIIGGGD HHCCCCCEEEECCCC	16.50	22369663
374	Phosphorylation	VIIGGGDTATVAKKY EEECCCCHHHHHHHH	27.74	22369663
376	Phosphorylation	IGGGDTATVAKKYGV ECCCCHHHHHHHHCC	24.08	22369663
379	Acetylation	GDTATVAKKYGVTDK CCHHHHHHHHCCCCC	42.04	24489116
379	Succinylation	GDTATVAKKYGVTDK CCHHHHHHHHCCCCC	42.04	23954790
379	Ubiquitination	GDTATVAKKYGVTDK CCHHHHHHHHCCCCC	42.04	22817900
380	2-Hydroxyisobutyrylation	DTATVAKKYGVTDKI CHHHHHHHHCCCCCC	37.18	-
380	Acetylation	DTATVAKKYGVTDKI CHHHHHHHHCCCCCC	37.18	24489116
380	Ubiquitination	DTATVAKKYGVTDKI CHHHHHHHHCCCCCC	37.18	23749301
386	Acetylation	KKYGVTDKISHVSTG HHHCCCCCCEEEECC	36.42	24489116
388	Phosphorylation	YGVTDKISHVSTGGG HCCCCCCEEEECCCC	24.47	22369663
391	Phosphorylation	TDKISHVSTGGGASL CCCCEEEECCCCCCE	18.86	22369663
392	Phosphorylation	DKISHVSTGGGASLE CCCEEEECCCCCCEE	38.46	22369663
397	Phosphorylation	VSTGGGASLELLEGK EECCCCCCEEHHCCC	26.17	22369663
404	Acetylation	SLELLEGKELPGVAF CEEHHCCCCCCCEEE	46.86	24489116
413	Phosphorylation	LPGVAFLSEKK---- CCCEEEEECCC----	40.70	22369663
415	Acetylation	GVAFLSEKK------ CEEEEECCC------	61.48	24489116
415	Succinylation	GVAFLSEKK------ CEEEEECCC------	61.48	23954790
416	2-Hydroxyisobutyrylation	VAFLSEKK------- EEEEECCC-------	62.61	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of PGK_YEAST !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of PGK_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PGK_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
COPA_YEAST	COP1	physical	11805826
COPB2_YEAST	SEC27	physical	11805826
LSM1_YEAST	LSM1	physical	17513695
LSM2_YEAST	LSM2	physical	17513695
LSM3_YEAST	LSM3	physical	17513695
LSM4_YEAST	LSM4	physical	17513695
LSM5_YEAST	LSM5	physical	17513695
LSM6_YEAST	LSM6	physical	17513695
LSM7_YEAST	LSM7	physical	17513695
PAT1_YEAST	PAT1	physical	17513695
KCC4_YEAST	KCC4	genetic	19269370
PGK_YEAST	PGK1	physical	16353200
CSG2_YEAST	CSG2	genetic	20093466
EFM4_YEAST	EFM4	genetic	20093466
MET28_YEAST	MET28	genetic	20093466
SDHX_YEAST	YJL045W	genetic	20093466
YJ24_YEAST	KCH1	genetic	20093466
ADE_YEAST	AAH1	genetic	20093466
BUD21_YEAST	BUD21	genetic	20093466
NAM7_YEAST	NAM7	physical	23928302
NMD2_YEAST	NMD2	physical	23928302
UPF3_YEAST	UPF3	physical	23928302
HSP71_YEAST	SSA1	physical	23928302

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PGK_YEAST

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Quantitative phosphoproteomics applied to the yeast pheromonesignaling pathway."; Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J.,Mann M., Jensen O.N.; Mol. Cell. Proteomics 4:310-327(2005). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION AT SER-4,AND MASS SPECTROMETRY.	15665377 [Abstract]
Phosphorylation
Reference	PubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; SER-8; SER-76;THR-93; SER-114; THR-203; THR-241; THR-331; THR-376; SER-391 ANDTHR-392, AND MASS SPECTROMETRY.	18407956 [Abstract]
"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391 AND SER-413, ANDMASS SPECTROMETRY.	17563356 [Abstract]
"Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry."; Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36; SER-110; SER-114;SER-130 AND SER-397, AND MASS SPECTROMETRY.	17287358 [Abstract]
"Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae."; Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.; J. Proteome Res. 6:1190-1197(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-154; THR-331 ANDTHR-392, AND MASS SPECTROMETRY.	17330950 [Abstract]
"Quantitative phosphoproteomics applied to the yeast pheromonesignaling pathway."; Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J.,Mann M., Jensen O.N.; Mol. Cell. Proteomics 4:310-327(2005). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION AT SER-4,AND MASS SPECTROMETRY.	15665377 [Abstract]