dbPTM

RPN2_YEAST - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RPN2_YEAST
UniProt AC	P32565
Protein Name	26S proteasome regulatory subunit RPN2
Gene Name	RPN2
Organism	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length	945
Subcellular Localization
Protein Description	Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins..
Protein Sequence	MSLTTAAPLLALLRENQDSVKTYALESINNVVDQLWSEISNELPDIEALYDDDTFSDREMAALIASKVYYNLGEYESAVKYALAAKDRFDIDEKSQFVETIVSKSIEMYVQEASKQYTKDEQFYTKDIIDPKLTSIFERMIEKCLKASELKLALGIALEGYRLDIIESALKSKLDQDSTSENVKIINYLLTLAITTVTNSKFRSSILRKSFDFLMNMPNCDYLTLNKVVVNLNDAGLALQLFKKLKEENDEGLSAQIAFDLVSSASQQLLEILVTELTAQGYDPALLNILSGLPTCDYYNTFLLNNKNIDIGLLNKSKSSLDGKFSLFHTAVSVANGFMHAGTTDNSFIKANLPWLGKAQNWAKFTATASLGVIHKGNLLEGKKVMAPYLPGSRASSRFIKGGSLYGLGLIYAGFGRDTTDYLKNIIVENSGTSGDEDVDVLLHGASLGIGLAAMGSANIEVYEALKEVLYNDSATSGEAAALGMGLCMLGTGKPEAIHDMFTYSQETQHGNITRGLAVGLALINYGRQELADDLITKMLASDESLLRYGGAFTIALAYAGTGNNSAVKRLLHVAVSDSNDDVRRAAVIALGFVLLRDYTTVPRIVQLLSKSHNAHVRCGTAFALGIACAGKGLQSAIDVLDPLTKDPVDFVRQAAMIALSMILIQQTEKLNPQVADINKNFLSVITNKHQEGLAKFGACVAQGIMNAGGRNVTIQLENADTGTLDTKSVVGLVMFSQFWYWFPLAHFLSLSFTPTTVIGIRGSDQAIPKFQMNCYAKEDAFSYPRMYEEASGKEVEKVATAVLSTTARAKARAKKTKKEKGPNEEEKKKEHEEKEKERETNKKGIKETKENDEEFYKNKYSSKPYKVDNMTRILPQQSRYISFIKDDRFVPVRKFKGNNGVVVLRDREPKEPVALIETVRQMKDVNAPLPTPFKVDDNVDFPSA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSLTTAAPL ------CCHHHHHHH	34.13	30377154
2	Acetylation	------MSLTTAAPL ------CCHHHHHHH	34.13	22814378
56	Phosphorylation	LYDDDTFSDREMAAL HCCCCCCCHHHHHHH	37.54	28889911
67	Ubiquitination	MAALIASKVYYNLGE HHHHHHHHHHHCCCC	25.82	17644757
80	Ubiquitination	GEYESAVKYALAAKD CCHHHHHHHHHHCCC	25.23	17644757
94	Ubiquitination	DRFDIDEKSQFVETI CCCCCCCHHHHHHHH	45.89	17644757
94	Acetylation	DRFDIDEKSQFVETI CCCCCCCHHHHHHHH	45.89	24489116
104	Ubiquitination	FVETIVSKSIEMYVQ HHHHHHHHHHHHHHH	44.55	17644757
105	Phosphorylation	VETIVSKSIEMYVQE HHHHHHHHHHHHHHH	19.10	28889911
126	Acetylation	KDEQFYTKDIIDPKL CCCCCCCCCCCCHHH	35.23	24489116
126	Succinylation	KDEQFYTKDIIDPKL CCCCCCCCCCCCHHH	35.23	23954790
132	Acetylation	TKDIIDPKLTSIFER CCCCCCHHHHHHHHH	61.73	24489116
146	Ubiquitination	RMIEKCLKASELKLA HHHHHHHHHHHHHHH	60.38	23749301
151	Ubiquitination	CLKASELKLALGIAL HHHHHHHHHHHHHHH	27.88	17644757
168	Phosphorylation	YRLDIIESALKSKLD HHHHHHHHHHHHCCC	28.61	22369663
171	Ubiquitination	DIIESALKSKLDQDS HHHHHHHHHCCCCCC	45.31	17644757
173	Ubiquitination	IESALKSKLDQDSTS HHHHHHHCCCCCCCH	55.27	23749301
227	Ubiquitination	CDYLTLNKVVVNLND CCEEEECEEEECCCH	39.25	17644757
243	Ubiquitination	GLALQLFKKLKEEND HHHHHHHHHHHHHCC	66.83	17644757
244	Ubiquitination	LALQLFKKLKEENDE HHHHHHHHHHHHCCC	58.41	17644757
307	Ubiquitination	NTFLLNNKNIDIGLL CHHHCCCCCEEEEEE	54.96	17644757
316	Acetylation	IDIGLLNKSKSSLDG EEEEEECCCCCCCCC	60.26	24489116
316	Ubiquitination	IDIGLLNKSKSSLDG EEEEEECCCCCCCCC	60.26	17644757
319	Phosphorylation	GLLNKSKSSLDGKFS EEECCCCCCCCCCHH	43.13	30377154
364	Ubiquitination	GKAQNWAKFTATASL CCCCCHHHHHEEEEE	34.56	17644757
376	Methylation	ASLGVIHKGNLLEGK EEECEEECCCHHCCC	38.07	20137074
376	Ubiquitination	ASLGVIHKGNLLEGK EEECEEECCCHHCCC	38.07	17644757
383	Ubiquitination	KGNLLEGKKVMAPYL CCCHHCCCEEECCCC	32.59	17644757
384	Ubiquitination	GNLLEGKKVMAPYLP CCHHCCCEEECCCCC	48.37	17644757
389	Phosphorylation	GKKVMAPYLPGSRAS CCEEECCCCCCCCCC	18.91	30377154
396	Phosphorylation	YLPGSRASSRFIKGG CCCCCCCCCCCCCCC	22.42	30377154
397	Phosphorylation	LPGSRASSRFIKGGS CCCCCCCCCCCCCCC	30.17	30377154
401	Ubiquitination	RASSRFIKGGSLYGL CCCCCCCCCCCEECC	55.28	17644757
471	Phosphorylation	EALKEVLYNDSATSG HHHHHHHCCCCCCHH	23.31	27017623
538	Acetylation	LADDLITKMLASDES HHHHHHHHHHHCCHH	25.56	24489116
538	Ubiquitination	LADDLITKMLASDES HHHHHHHHHHHCCHH	25.56	24961812
569	Ubiquitination	TGNNSAVKRLLHVAV CCCCHHHHHHHHHHH	36.72	17644757
611	Ubiquitination	RIVQLLSKSHNAHVR HHHHHHHHCCCCCCC	56.56	22106047
632	Ubiquitination	LGIACAGKGLQSAID HHHHHCCCCHHHHHH	37.61	17644757
646	Acetylation	DVLDPLTKDPVDFVR HHHCCCCCCHHHHHH	68.65	24489116
646	Ubiquitination	DVLDPLTKDPVDFVR HHHCCCCCCHHHHHH	68.65	17644757
680	Acetylation	PQVADINKNFLSVIT HHHHHCCHHHHHHHC	49.78	24489116
680	Ubiquitination	PQVADINKNFLSVIT HHHHHCCHHHHHHHC	49.78	17644757
684	Phosphorylation	DINKNFLSVITNKHQ HCCHHHHHHHCCCCH	13.82	22369663
687	Phosphorylation	KNFLSVITNKHQEGL HHHHHHHCCCCHHHH	35.74	22369663
689	Ubiquitination	FLSVITNKHQEGLAK HHHHHCCCCHHHHHH	37.71	17644757
689	Acetylation	FLSVITNKHQEGLAK HHHHHCCCCHHHHHH	37.71	24489116
696	Ubiquitination	KHQEGLAKFGACVAQ CCHHHHHHHHHHHHH	49.74	17644757
770	Acetylation	GSDQAIPKFQMNCYA CCCCCCCHHCCEEEE	41.96	24489116
770	Ubiquitination	GSDQAIPKFQMNCYA CCCCCCCHHCCEEEE	41.96	17644757
778	Ubiquitination	FQMNCYAKEDAFSYP HCCEEEECCCHHCCH	29.65	17644757
794	Ubiquitination	MYEEASGKEVEKVAT HHHHHCCCHHHHHHH	56.97	23749301
798	Ubiquitination	ASGKEVEKVATAVLS HCCCHHHHHHHHHHH	42.20	22817900
801	Phosphorylation	KEVEKVATAVLSTTA CHHHHHHHHHHHHHH	21.93	20486117
805	Phosphorylation	KVATAVLSTTARAKA HHHHHHHHHHHHHHH	19.67	30377154
806	Phosphorylation	VATAVLSTTARAKAR HHHHHHHHHHHHHHH	21.95	28889911
807	Phosphorylation	ATAVLSTTARAKARA HHHHHHHHHHHHHHH	15.69	25752575
818	Ubiquitination	KARAKKTKKEKGPNE HHHHHHHHHHCCCCH	68.74	17644757
819	Ubiquitination	ARAKKTKKEKGPNEE HHHHHHHHHCCCCHH	70.64	17644757
821	Ubiquitination	AKKTKKEKGPNEEEK HHHHHHHCCCCHHHH	84.37	17644757
828	Ubiquitination	KGPNEEEKKKEHEEK CCCCHHHHHHHHHHH	72.87	17644757
829	Ubiquitination	GPNEEEKKKEHEEKE CCCHHHHHHHHHHHH	67.67	17644757
830	Ubiquitination	PNEEEKKKEHEEKEK CCHHHHHHHHHHHHH	75.31	17644757
835	Ubiquitination	KKKEHEEKEKERETN HHHHHHHHHHHHHHH	71.72	17644757
837	Ubiquitination	KEHEEKEKERETNKK HHHHHHHHHHHHHHH	73.47	17644757
843	Ubiquitination	EKERETNKKGIKETK HHHHHHHHHCHHHHH	60.85	17644757
844	Ubiquitination	KERETNKKGIKETKE HHHHHHHHCHHHHHH	68.86	17644757
847	Ubiquitination	ETNKKGIKETKENDE HHHHHCHHHHHHCHH	68.92	17644757
850	Acetylation	KKGIKETKENDEEFY HHCHHHHHHCHHHHH	56.46	24489116
850	Ubiquitination	KKGIKETKENDEEFY HHCHHHHHHCHHHHH	56.46	17644757
857	Phosphorylation	KENDEEFYKNKYSSK HHCHHHHHHHCCCCC	19.55	27717283
858	Ubiquitination	ENDEEFYKNKYSSKP HCHHHHHHHCCCCCC	52.89	17644757
860	Ubiquitination	DEEFYKNKYSSKPYK HHHHHHHCCCCCCEE	42.21	17644757
864	Ubiquitination	YKNKYSSKPYKVDNM HHHCCCCCCEECCCC	46.56	17644757
867	Ubiquitination	KYSSKPYKVDNMTRI CCCCCCEECCCCCCC	52.19	17644757
883	Phosphorylation	PQQSRYISFIKDDRF CCHHHHEEEECCCCE	16.40	28889911
886	Ubiquitination	SRYISFIKDDRFVPV HHHEEEECCCCEEEE	52.12	17644757
886	Acetylation	SRYISFIKDDRFVPV HHHEEEECCCCEEEE	52.12	24489116
895	Ubiquitination	DRFVPVRKFKGNNGV CCEEEEEEEECCCCE	52.22	17644757
911	Ubiquitination	VLRDREPKEPVALIE EEECCCCCCCEEHHH	70.65	17644757
911	Acetylation	VLRDREPKEPVALIE EEECCCCCCCEEHHH	70.65	24489116
924	Ubiquitination	IETVRQMKDVNAPLP HHHHHHHCCCCCCCC	50.62	17644757
924	Acetylation	IETVRQMKDVNAPLP HHHHHHHCCCCCCCC	50.62	24489116
932	Phosphorylation	DVNAPLPTPFKVDDN CCCCCCCCCCCCCCC	49.38	27214570
935	Ubiquitination	APLPTPFKVDDNVDF CCCCCCCCCCCCCCC	46.05	17644757

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of RPN2_YEAST !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RPN2_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RPN2_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UBR1_YEAST	UBR1	physical	10688918
SEM1_YEAST	SEM1	physical	15572408
RPN12_YEAST	RPN12	physical	15572408
HSC82_YEAST	HSC82	genetic	12853471
NOT1_YEAST	CDC39	physical	16554755
SIR4_YEAST	SIR4	physical	16554755
PRS6B_YEAST	RPT3	physical	16554755
RPN3_YEAST	RPN3	physical	16554755
RPN12_YEAST	RPN12	physical	16554755
PSD10_YEAST	NAS6	physical	16554755
PRS7_YEAST	RPT1	physical	16554755
SN114_YEAST	SNU114	physical	16554755
RPN13_YEAST	RPN13	physical	16554755
SPG5_YEAST	SPG5	physical	16554755
PRS6A_YEAST	RPT5	physical	16554755
PRS10_YEAST	RPT4	physical	16554755
RPN8_YEAST	RPN8	physical	16554755
RPN7_YEAST	RPN7	physical	16554755
METK1_YEAST	SAM1	physical	16554755
UBX1_YEAST	SHP1	genetic	17314980
TEC1_YEAST	TEC1	genetic	17314980
HUL5_YEAST	HUL5	physical	17190603
RPN13_YEAST	RPN13	physical	18467557
PSA1_YEAST	SCL1	physical	18511945
PSA4_YEAST	PRE6	physical	18511945
PSA5_YEAST	PUP2	physical	18511945
PSA6_YEAST	PRE5	physical	18511945
PSA7_YEAST	PRE10	physical	18511945
HSP71_YEAST	SSA1	physical	19536198
SSB1_YEAST	SSB1	physical	19536198
RFU1_YEAST	RFU1	genetic	19410548
RPN10_YEAST	RPN10	genetic	18622397
RPN3_YEAST	RPN3	genetic	18622397
PRS8_YEAST	RPT6	genetic	18622397
PRS6B_YEAST	RPT3	genetic	18622397
PSB1_YEAST	PRE3	genetic	18622397
PRS4_YEAST	RPT2	genetic	18622397
RPN11_YEAST	RPN11	genetic	18622397
PSA2_YEAST	PRE8	genetic	18622397
PSB2_YEAST	PUP1	genetic	18622397
PSA6_YEAST	PRE5	genetic	18622397
PSA7_YEAST	PRE10	genetic	18622397
PSA5_YEAST	PUP2	genetic	18622397
PSMD9_YEAST	NAS2	physical	20471945
PRS6B_YEAST	RPT3	physical	22237024
PRS8_YEAST	RPT6	physical	22237024
RPN13_YEAST	RPN13	physical	22318722
PRS4_YEAST	RPT2	physical	22307589
PRS8_YEAST	RPT6	physical	22307589
RPN3_YEAST	RPN3	physical	22307589
RPN5_YEAST	RPN5	physical	22307589
RPN9_YEAST	RPN9	physical	22307589
PRS8_YEAST	RPT6	physical	22940862
HSP72_YEAST	SSA2	physical	22940862
RPN2_YEAST	RPN2	physical	22940862
RPN10_YEAST	RPN10	physical	22940862
RPN9_YEAST	RPN9	physical	22940862
RPN12_YEAST	RPN12	physical	22940862
HSP71_YEAST	SSA1	physical	22940862
PRS6A_YEAST	RPT5	physical	22940862
PRS6B_YEAST	RPT3	physical	22940862
RPN3_YEAST	RPN3	physical	22940862
PRS10_YEAST	RPT4	physical	22940862
RPN8_YEAST	RPN8	physical	22940862
RPN1_YEAST	RPN1	physical	22940862
RPN5_YEAST	RPN5	physical	22940862
RPN11_YEAST	RPN11	physical	22940862
RPN7_YEAST	RPN7	physical	22940862
PRS7_YEAST	RPT1	physical	22940862
RPN6_YEAST	RPN6	physical	22940862
UBP6_YEAST	UBP6	physical	22940862
PHO88_YEAST	PHO88	genetic	23891562
PFD3_YEAST	PAC10	genetic	23891562
CUZ1_YEAST	CUZ1	physical	24297164
RPN4_YEAST	RPN4	physical	11248031
PMP1_YEAST	PMP1	physical	26404137

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RPN2_YEAST

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"N-terminal modifications of the 19S regulatory particle subunits ofthe yeast proteasome."; Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H.; Arch. Biochem. Biophys. 409:341-348(2003). Cited for: PROTEIN SEQUENCE OF 2-9, AND ACETYLATION AT SER-2.	12504901 [Abstract]
Phosphorylation
Reference	PubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; THR-801; SER-883 ANDTHR-932, AND MASS SPECTROMETRY.	18407956 [Abstract]