| UniProt ID | TRA1_YEAST | |
|---|---|---|
| UniProt AC | P38811 | |
| Protein Name | Transcription-associated protein 1 | |
| Gene Name | TRA1 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 3744 | |
| Subcellular Localization | Nucleus . | |
| Protein Description | Essential component of histone acetyltransferase (HAT) complexes, which serves as a target for activators during recruitment of HAT complexes. Essential for vegetative growth. Functions as a component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.. | |
| Protein Sequence | MSLTEQIEQFASRFRDDDATLQSRYSTLSELYDIMELLNSPEDYHFFLQAVIPLLLNQLKEVPISYDAHSPEQKLRNSMLDIFNRCLMNQTFQPYAMEVLEFLLSVLPKENEENGILCMKVLTTLFKSFKSILQDKLDSFIRIIIQIYKNTPNLINQTFYEAGKAEQGDLDSPKEPQADELLDEFSKNDEEKDFPSKQSSTEPRFENSTSSNGLRSSMFSFKILSECPITMVTLYSSYKQLTSTSLPEFTPLIMNLLNIQIKQQQEAREQAESRGEHFTSISTEIINRPAYCDFILAQIKATSFLAYVFIRGYAPEFLQDYVNFVPDLIIRLLQDCPSELSSARKELLHATRHILSTNYKKLFLPKLDYLFDERILIGNGFTMHETLRPLAYSTVADFIHNIRSELQLSEIEKTIKIYTGYLLDESLALTVQIMSAKLLLNLVERILKLGKENPQEAPRAKKLLMIIIDSYMNRFKTLNRQYDTIMKYYGRYETHKKEKAEKLKNSIQDNDKESEEFMRKVLEPSDDDHLMPQPKKEDINDSPDVEMTESDKVVKNDVEMFDIKNYAPILLLPTPTNDPIKDAFYLYRTLMSFLKTIIHDLKVFNPPPNEYTVANPKLWASVSRVFSYEEVIVFKDLFHECIIGLKFFKDHNEKLSPETTKKHFDISMPSLPVSATKDARELMDYLAFMFMQMDNATFNEIIEQELPFVYERMLEDSGLLHVAQSFLTSEITSPNFAGILLRFLKGKLKDLGNVDFNTSNVLIRLFKLSFMSVNLFPNINEVVLLPHLNDLILNSLKYSTTAEEPLVYFYLIRTLFRSIGGGRFENLYRSIKPILQVLLQSLNQMILTARLPHERELYVELCITVPVRLSVLAPYLPFLMKPLVFALQQYPDLVSQGLRTLELCIDNLTAEYFDPIIEPVIDDVSKALFNLLQPQPFNHAISHNVVRILGKLGGRNRQFLKPPTDLTEKTELDIDAIADFKINGMPEDVPLSVTPGIQSALNILQSYKSDIHYRKSAYKYLTCVLLLMTKSSAEFPTNYTELLKTAVNSIKLERIGIEKNFDLEPTVNKRDYSNQENLFLRLLESVFYATSIKELKDDAMDLLNNLLDHFCLLQVNTTLLNKRNYNGTFNIDLKNPNFMLDSSLILDAIPFALSYYIPEVREVGVLAYKRIYEKSCLIYGEELALSHSFIPELAKQFIHLCYDETYYNKRGGVLGIKVLIDNVKSSSVFLKKYQYNLANGLLFVLKDTQSEAPSAITDSAEKLLIDLLSITFADVKEEDLGNKVLENTLTDIVCELSNANPKVRNACQKSLHTISNLTGIPIVKLMDHSKQFLLSPIFAKPLRALPFTMQIGNVDAITFCLSLPNTFLTFNEELFRLLQESIVLADAEDESLSTNIQKTTEYSTSEQLVQLRIACIKLLAIALKNEEFATAQQGNIRIRILAVFFKTMLKTSPEIINTTYEALKGSLAENSKLPKELLQNGLKPLLMNLSDHQKLTVPGLDALSKLLELLIAYFKVEIGRKLLDHLTAWCRVEVLDTLFGQDLAEQMPTKIIVSIINIFHLLPPQADMFLNDLLLKVMLLERKLRLQLDSPFRTPLARYLNRFHNPVTEYFKKNMTLRQLVLFMCNIVQRPEAKELAEDFEKELDNFYDFYISNIPKNQVRVVSFFTNMVDLFNTMVITNGDEWLKKKGNMILKLKDMLNLTLKTIKENSFYIDHLQLNQSIAKFQALYLRFTELSERDQNPLLLDFIDFSFSNGIKASYSLKKFIFHNIIASSNKEKQNNFINDATLFVLSDKCLDARIFVLKNVINSTLIYEVATSGSLKSYLVEDKKPKWLELLHNKIWKNSNAILAYDVLDHHDLFRFELLQLSAIFIKADPEIIAEIKKDIIKFCWNFIKLEDTLIKQSAYLVTSYFISKFDFPIKVVTQVFVALLRSSHVEARYLVKQSLDVLTPVLHERMNAAGTPDTWINWVKRVMVENSSSQNNILYQFLISHPDLFFNSRDLFISNIIHHMNKITFMSNSNSDSHTLAIDLASLILYWENKTLEITNVNNTKTDSDGDVVMSDSKSDINPVEADTTAIIVDANNNSPISLHLREACTAFLIRYVCASNHRAIETELGLRAINILSELISDKHWTNVNVKLVYFEKFLIFQDLDSENILYYCMNALDVLYVFFKNKTKEWIMENLPTIQNLLEKCIKSDHHDVQEALQKVLQVIMKAIKAQGVSVIIEEESPGKTFIQMLTSVITQDLQETSSVTAGVTLAWVLFMNFPDNIVPLLTPLMKTFSKLCKDHLSISQPKDAMALEEARITTKLLEKVLYILSLKVSLLGDSRRPFLSTVALLIDHSMDQNFLRKIVNMSRSWIFNTEIFPTVKEKAAILTKMLAFEIRGEPSLSKLFYEIVLKLFDQEHFNNTEITVRMEQPFLVGTRVEDIGIRKRFMTILDNSLERDIKERLYYVIRDQNWEFIADYPWLNQALQLLYGSFNREKELSLKNIYCLSPPSILQEYLPENAEMVTEVNDLELSNFVKGHIASMQGLCRIISSDFIDSLIEIFYQDPKAIHRAWVTLFPQVYKSIPKNEKYGFVRSIITLLSKPYHTRQISSRTNVINMLLDSISKIESLELPPHLVKYLAISYNAWYQSINILESIQSNTSIDNTKIIEANEDALLELYVNLQEEDMFYGLWRRRAKYTETNIGLSYEQIGLWDKAQQLYEVAQVKARSGALPYSQSEYALWEDNWIQCAEKLQHWDVLTELAKHEGFTDLLLECGWRVADWNSDRDALEQSVKSVMDVPTPRRQMFKTFLALQNFAESRKGDQEVRKLCDEGIQLSLIKWVSLPIRYTPAHKWLLHGFQQYMEFLEATQIYANLHTTTVQNLDSKAQEIKRILQAWRDRLPNTWDDVNMWNDLVTWRQHAFQVINNAYLPLIPALQQSNSNSNINTHAYRGYHEIAWVINRFAHVARKHNMPDVCISQLARIYTLPNIEIQEAFLKLREQAKCHYQNMNELTTGLDVISNTNLVYFGTVQKAEFFTLKGMFLSKLRAYEEANQAFATAVQIDLNLAKAWAQWGFFNDRRLSEEPNNISFASNAISCYLQAAGLYKNSKIRELLCRILWLISIDDASGMLTNAFDSFRGEIPVWYWITFIPQLLTSLSHKEANMVRHILIRIAKSYPQALHFQLRTTKEDFAVIQRQTMAVMGDKPDTNDRNGRRQPWEYLQELNNILKTAYPLLALSLESLVAQINDRFKSTTDEDLFRLINVLLIDGTLNYNRLPFPRKNPKLPENTEKNLVKFSTTLLAPYIRPKFNADFIDNKPDYETYIKRLRYWRRRLENKLDRASKKENLEVLCPHLSNFHHQKFEDIEIPGQYLLNKDNNVHFIKIARFLPTVDFVRGTHSSYRRLMIRGHDGSVHSFAVQYPAVRHSRREERMFQLYRLFNKSLSKNVETRRRSIQFNLPIAIPLSPQVRIMNDSVSFTTLHEIHNEFCKKKGFDPDDIQDFMADKLNAAHDDALPAPDMTILKVEIFNSIQTMFVPSNVLKDHFTSLFTQFEDFWLFRKQFASQYSSFVFMSYMMMINNRTPHKIHVDKTSGNVFTLEMLPSRFPYERVKPLLKNHDLSLPPDSPIFHNNEPVPFRLTPNIQSLIGDSALEGIFAVNLFTISRALIEPDNELNTYLALFIRDEIISWFSNLHRPIIENPQLREMVQTNVDLIIRKVAQLGHLNSTPTVTTQFILDCIGSAVSPRNLARTDVNFMPWF | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 2 | Acetylation | ------MSLTEQIEQ ------CCHHHHHHH | 56.13 | 22814378 | |
| 4 | Phosphorylation | ----MSLTEQIEQFA ----CCHHHHHHHHH | 34.82 | 30377154 | |
| 12 | Phosphorylation | EQIEQFASRFRDDDA HHHHHHHHHHCCCHH | 32.99 | 30377154 | |
| 74 | Acetylation | DAHSPEQKLRNSMLD CCCCHHHHHHHHHHH | 47.99 | 24489116 | |
| 172 | Phosphorylation | AEQGDLDSPKEPQAD CCCCCCCCCCCCCHH | 45.21 | 22369663 | |
| 186 | Phosphorylation | DELLDEFSKNDEEKD HHHHHHHCCCHHHCC | 28.62 | 29136822 | |
| 196 | Phosphorylation | DEEKDFPSKQSSTEP HHHCCCCCCCCCCCC | 43.23 | 24961812 | |
| 199 | Phosphorylation | KDFPSKQSSTEPRFE CCCCCCCCCCCCCCC | 42.85 | 24961812 | |
| 200 | Phosphorylation | DFPSKQSSTEPRFEN CCCCCCCCCCCCCCC | 34.01 | 24961812 | |
| 201 | Phosphorylation | FPSKQSSTEPRFENS CCCCCCCCCCCCCCC | 56.08 | 20377248 | |
| 208 | Phosphorylation | TEPRFENSTSSNGLR CCCCCCCCCCCCCCC | 23.59 | 24961812 | |
| 209 | Phosphorylation | EPRFENSTSSNGLRS CCCCCCCCCCCCCCH | 47.99 | 24961812 | |
| 210 | Phosphorylation | PRFENSTSSNGLRSS CCCCCCCCCCCCCHH | 22.94 | 24961812 | |
| 211 | Phosphorylation | RFENSTSSNGLRSSM CCCCCCCCCCCCHHH | 34.70 | 24961812 | |
| 216 | Phosphorylation | TSSNGLRSSMFSFKI CCCCCCCHHHHHHHH | 31.06 | 24961812 | |
| 217 | Phosphorylation | SSNGLRSSMFSFKIL CCCCCCHHHHHHHHH | 19.88 | 24961812 | |
| 220 | Phosphorylation | GLRSSMFSFKILSEC CCCHHHHHHHHHHCC | 19.59 | 24961812 | |
| 230 | Phosphorylation | ILSECPITMVTLYSS HHHCCCCEEEEEHHH | 7.25 | 27017623 | |
| 506 | Phosphorylation | KAEKLKNSIQDNDKE HHHHHHHHHCCCHHH | 21.75 | 30377154 | |
| 542 | Phosphorylation | KKEDINDSPDVEMTE CCCCCCCCCCCCCCC | 20.64 | 22369663 | |
| 548 | Phosphorylation | DSPDVEMTESDKVVK CCCCCCCCCCCCCCC | 20.87 | 21440633 | |
| 552 | Acetylation | VEMTESDKVVKNDVE CCCCCCCCCCCCCHH | 59.20 | 23572591 | |
| 602 | Acetylation | KTIIHDLKVFNPPPN HHHHHHHCCCCCCCC | 51.33 | 24489116 | |
| 1059 | Acetylation | LERIGIEKNFDLEPT HHCCCCCCCCCCCCC | 62.08 | 24489116 | |
| 1248 | Phosphorylation | LLFVLKDTQSEAPSA EEEEEECCCCCCCCC | 32.19 | 29688323 | |
| 1250 | Phosphorylation | FVLKDTQSEAPSAIT EEEECCCCCCCCCCC | 37.51 | 27017623 | |
| 1254 | Phosphorylation | DTQSEAPSAITDSAE CCCCCCCCCCCHHHH | 39.07 | 27017623 | |
| 1257 | Phosphorylation | SEAPSAITDSAEKLL CCCCCCCCHHHHHHH | 24.50 | 27017623 | |
| 1259 | Phosphorylation | APSAITDSAEKLLID CCCCCCHHHHHHHHH | 29.67 | 29688323 | |
| 1269 | Phosphorylation | KLLIDLLSITFADVK HHHHHHHHCCCCCCC | 27.21 | 29688323 | |
| 1271 | Phosphorylation | LIDLLSITFADVKEE HHHHHHCCCCCCCHH | 14.92 | 29688323 | |
| 1297 | Phosphorylation | TDIVCELSNANPKVR HHHHHHHHCCCHHHH | 16.17 | 28889911 | |
| 1310 | Phosphorylation | VRNACQKSLHTISNL HHHHHHHHHHHHHHC | 10.25 | 19779198 | |
| 1313 | Phosphorylation | ACQKSLHTISNLTGI HHHHHHHHHHHCCCC | 31.24 | 19779198 | |
| 1315 | Phosphorylation | QKSLHTISNLTGIPI HHHHHHHHHCCCCCE | 27.26 | 19779198 | |
| 1318 | Phosphorylation | LHTISNLTGIPIVKL HHHHHHCCCCCEEEE | 37.00 | 19779198 | |
| 1430 | Phosphorylation | LKNEEFATAQQGNIR HCCHHHHHHHCCCHH | 30.25 | 28889911 | |
| 1466 | Phosphorylation | TYEALKGSLAENSKL HHHHHHCCHHHCCCC | 23.87 | 17563356 | |
| 1840 | Acetylation | WLELLHNKIWKNSNA HHHHHHHHHHCCCCE | 38.91 | 24489116 | |
| 2053 | Phosphorylation | TNVNNTKTDSDGDVV EECCCCCCCCCCCEE | 38.40 | 28889911 | |
| 2055 | Phosphorylation | VNNTKTDSDGDVVMS CCCCCCCCCCCEEEC | 49.04 | 27214570 | |
| 2062 | Phosphorylation | SDGDVVMSDSKSDIN CCCCEEECCCCCCCC | 27.42 | 27214570 | |
| 2064 | Phosphorylation | GDVVMSDSKSDINPV CCEEECCCCCCCCCC | 27.10 | 28889911 | |
| 2588 | Phosphorylation | RSIITLLSKPYHTRQ HHHHHHHCCCCHHHC | 34.40 | 30377154 | |
| 2593 | Phosphorylation | LLSKPYHTRQISSRT HHCCCCHHHCCHHHH | 20.58 | 30377154 | |
| 2611 | Phosphorylation | NMLLDSISKIESLEL HHHHHHHHHHHHCCC | 31.37 | 19779198 | |
| 2788 | Phosphorylation | KSVMDVPTPRRQMFK HHHHCCCCCHHHHHH | 29.31 | 25752575 | |
| 3175 | Acetylation | HFQLRTTKEDFAVIQ HEEECCCHHHHHEEE | 55.46 | 24489116 | |
| 3282 | Acetylation | NTEKNLVKFSTTLLA CCHHCCCHHHHHHHH | 36.44 | 24489116 | |
| 3307 | Phosphorylation | FIDNKPDYETYIKRL CCCCCCCHHHHHHHH | 20.78 | 27017623 | |
| 3386 | Phosphorylation | DFVRGTHSSYRRLMI CCCCCCCCCHHEEEE | 28.98 | 25521595 | |
| 3432 | Ubiquitination | LFNKSLSKNVETRRR HHHHHHCCCHHHHHH | 71.01 | 23749301 | |
| 3440 | Phosphorylation | NVETRRRSIQFNLPI CHHHHHHHHCCCCCE | 20.94 | 21440633 | |
| 3550 | Phosphorylation | LFRKQFASQYSSFVF HHHHHHHHHHHHHHH | 30.82 | 30377154 | |
| 3559 | Phosphorylation | YSSFVFMSYMMMINN HHHHHHHHHHHHHCC | 9.83 | 30377154 |
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of TRA1_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of TRA1_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172; SER-542 ANDTHR-2788, AND MASS SPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172 AND SER-1466, ANDMASS SPECTROMETRY. | |
