MLC1_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MLC1_YEAST
• UniProt AC: P53141
• Protein Name: Myosin light chain 1
• Gene Name: MLC1
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 149
• Subcellular Localization: Bud neck . Bud tip . Concentrates to sites of polarized growth, namely to the incipient bud site in G1, to the bud tip during S and G2 phases of the cell cycle and to the bud neck during cytokinesis.
• Protein Description: Essential light chain for the class II conventional myosin MYO1. Acts also as light chain for the class V unconventional myosin MYO2 and for IQG1. Involved in the assembly of the contractile actomyosin ring at the bud neck during cytokinesis by recruiting IQG1 to the bud neck. Also required for chitin and MYO2-dependent secretory vesicle deposition to the center of the bud neck for septum formation. May stabilize MYO2 by binding to its IQ domains. Its major function is probably not to regulate MYO1 activity, but rather to coordinate actin ring formation and targeted membrane deposition during cytokinesis via its interactions with MYO1, IQG1 and MYO2..
• Protein Sequence: MSATRANKDIFTLFDKKGQGAIAKDSLGDYLRAIGYNPTNQLVQDIINADSSLRDASSLTLDQITGLIEVNEKELDATTKAKTEDFVKAFQVFDKESTGKVSVGDLRYMLTGLGEKLTDAEVDELLKGVEVDSNGEIDYKKFIEDVLRQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSATRANKD ------CCCCCCCCC	38.04	30377154
4	Phosphorylation	----MSATRANKDIF ----CCCCCCCCCCE	24.02	30377154
8	Acetylation	MSATRANKDIFTLFD CCCCCCCCCCEECCC	51.74	24489116
16	Acetylation	DIFTLFDKKGQGAIA CCEECCCCCCCCCHH	52.01	24489116
17	Acetylation	IFTLFDKKGQGAIAK CEECCCCCCCCCHHH	59.31	24489116
24	Acetylation	KGQGAIAKDSLGDYL CCCCCHHHCCHHHHH	41.56	24489116
39	Phosphorylation	RAIGYNPTNQLVQDI HHCCCCCHHHHHHHH	31.77	24909858
51	Phosphorylation	QDIINADSSLRDASS HHHHHCCCHHHHCHH	28.94	30377154
57	Phosphorylation	DSSLRDASSLTLDQI CCHHHHCHHCCHHHH	30.12	28132839
58	Phosphorylation	SSLRDASSLTLDQIT CHHHHCHHCCHHHHC	27.39	28132839
60	Phosphorylation	LRDASSLTLDQITGL HHHCHHCCHHHHCCE	30.18	29734811
73	Acetylation	GLIEVNEKELDATTK CEEEECHHHCCCCCC	59.37	24489116
82	2-Hydroxyisobutyrylation	LDATTKAKTEDFVKA CCCCCCCCHHHHHHH	54.74	-
83	Phosphorylation	DATTKAKTEDFVKAF CCCCCCCHHHHHHHH	45.72	25752575
88	Ubiquitination	AKTEDFVKAFQVFDK CCHHHHHHHHHEECC	43.40	24961812
95	Acetylation	KAFQVFDKESTGKVS HHHHEECCCCCCCEE	41.94	24489116
102	Phosphorylation	KESTGKVSVGDLRYM CCCCCCEEHHHHHHH	24.70	30377154
108	Phosphorylation	VSVGDLRYMLTGLGE EEHHHHHHHHHCCHH	12.08	28889911
111	Phosphorylation	GDLRYMLTGLGEKLT HHHHHHHHCCHHHCC	17.52	28889911
116	Ubiquitination	MLTGLGEKLTDAEVD HHHCCHHHCCHHHHH	55.69	22106047
133	Phosphorylation	LKGVEVDSNGEIDYK HCCCEECCCCCCCHH	52.49	22369663
139	Phosphorylation	DSNGEIDYKKFIEDV CCCCCCCHHHHHHHH	23.03	22369663

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MLC1_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MLC1_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MLC1_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MYO2_YEAST	MYO2	physical	12456647
SEC4_YEAST	SEC4	physical	12456647
IQG1_YEAST	IQG1	physical	11082046
MYO1_YEAST	MYO1	physical	11082046
MYO2_YEAST	MYO2	physical	12351846
MYO2_YEAST	MYO2	physical	12554638
MYO1_YEAST	MYO1	physical	15210731
MYO2_YEAST	MYO2	physical	16120677
MYO1_YEAST	MYO1	genetic	12456647
MYO2_YEAST	MYO2	genetic	12456647
MYO2_YEAST	MYO2	genetic	9700160
MYO4_YEAST	MYO4	physical	16554755
CALM_YEAST	CMD1	physical	16554755
HSP42_YEAST	HSP42	physical	16554755
RV167_YEAST	RVS167	physical	16554755
PIL1_YEAST	PIL1	physical	16554755
CORO_YEAST	CRN1	physical	16554755
IF4E_YEAST	CDC33	physical	16554755
MYO2_YEAST	MYO2	physical	16554755
LSP1_YEAST	LSP1	physical	16554755
BSP1_YEAST	BSP1	physical	16554755
MYO2_YEAST	MYO2	physical	16468996
IQG1_YEAST	IQG1	physical	10873803
MLC1_YEAST	MLC1	physical	18719252
MYO2_YEAST	MYO2	physical	18221262
MYO1_YEAST	MYO1	physical	21757693
MYO2_YEAST	MYO2	physical	21757693
IQG1_YEAST	IQG1	physical	24895401
CYK2_YEAST	HOF1	physical	24895401
IQGA1_HUMAN	IQGAP1	physical	18587628
K1683_HUMAN	KIAA1683	physical	27107014

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.