| UniProt ID | IRA2_YEAST | |
|---|---|---|
| UniProt AC | P19158 | |
| Protein Name | Inhibitory regulator protein IRA2 | |
| Gene Name | IRA2 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 3079 | |
| Subcellular Localization | Cytoplasm . | |
| Protein Description | Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase activity of Ras proteins.. | |
| Protein Sequence | MSQPTKNKKKEHGTDSKSSRMTRTLVNHILFERILPILPVESNLSTYSEVEEYSSFISCRSVLINVTVSRDANAMVEGTLELIESLLQGHEIISDKGSSDVIESILIILRLLSDALEYNWQNQESLHYNDISTHVEHDQEQKYRPKLNSILPDYSSTHSNGNKHFFHQSKPQALIPELASKLLESCAKLKFNTRTLQILQNMISHVHGNILTTLSSSILPRHKSYLTRHNHPSHCKMIDSTLGHILRFVAASNPSEYFEFIRKSVQVPVTQTHTHSHSHSHSLPSSVYNSIVPHFDLFSFIYLSKHNFKKYLELIKNLSVTLRKTIYHCLLLHYSAKAIMFWIMARPAEYYELFNLLKDNNNEHSKSLNTLNHTLFEEIHSTFNVNSMITTNQNAHQGSSSPSSSSPSSPPSSSSSDNNNQNIIAKSLSRQLSHHQSYIQQQSERKLHSSWTTNSQSSTSLSSSTSNSTTTDFSTHTQPGEYDPSLPDTPTMSNITISASSLLSQTPTPTTQLQQRLNSAAAAAAAAASPSNSTPTGYTAEQQSRASYDAHKTGHTGKDYDEHFLSVTRLDNVLELYTHFDDTEVLPHTSVLKFLTTLTMFDIDLFNELNATSFKYIPDCTMHRPKERTSSFNNTAHETGSEKTSGIKHITQGLKKLTSLPSSTKKTVKFVKMLLRNLNGNQAVSDVALLDTMRALLSFFTMTSAVFLVDRNLPSVLFAKRLIPIMGTNLSVGQDWNSKINNSLMVCLKKNSTTFVQLQLIFFSSAIQFDHELLLARLSIDTMANNLNMQKLCLYTEGFRIFFDIPSKKELRKAIAVKISKFFKTLFSIIADILLQEFPYFDEQITDIVASILDGTIINEYGTKKHFKGSSPSLCSTTRSRSGSTSQSSMTPVSPLGLDTDICPMNTLSLVGSSTSRNSDNVNSLNSSPKNLSSDPYLSHLVAPRARHALGGPSSIIRNKIPTTLTSPPGTEKSSPVQRPQTESISATPMAITNSTPLSSAAFGIRSPLQKIRTRRYSDESLGKFMKSTNNYIQEHLIPKDLNEATLQDARRIMINIFSIFKRPNSYFIIPHNINSNLQWVSQDFRNIMKPIFVAIVSPDVDLQNTAQSFMDTLLSNVITYGESDENISIEGYHLLCSYTVTLFAMGLFDLKINNEKRQILLDITVKFMKVRSHLAGIAEASHHMEYISDSEKLTFPLIMGTVGRALFVSLYSSQQKIEKTLKIAYTEYLSAINFHERNIDDADKTWVHNIEFVEAMCHDNYTTSGSIAFQRRTRNNILRFATIPNAILLDSMRMIYKKWHTYTHSKSLEKQERNDFRNFAGILASLSGILFINKKILQEMYPYLLDTVSELKKNIDSFISKQCQWLNYPDLLTRENSRDILSVELHPLSFNLLFNNLRLKLKELACSDLSIPENESSYVLLEQIIKMLRTILGRDDDNYVMMLFSTEIVDLIDLLTDEIKKIPAYCPKYLKAIIQMTKMFSALQHSEVNLGVKNHFHVKNKWLRQITDWFQVSIAREYDFENLSKPLKEMDLVKRDMDILYIDTAIEASTAIAYLTRHTFLEIPPAASDPELSRSRSVIFGFYFNILMKGLEKSSDRDNYPVFLRHKMSVLNDNVILSLTNLSNTNVDASLQFTLPMGYSGNRNIRNAFLEVFINIVTNYRTYTAKTDLGKLEAADKFLRYTIEHPQLSSFGAAVCPASDIDAYAAGLINAFETRNATHIVVAQLIKNEIEKSSRPTDILRRNSCATRSLSMLARSKGNEYLIRTLQPLLKKIIQNRDFFEIEKLKPEDSDAERQIELFVKYMNELLESISNSVSYFPPPLFYICQNIYKVACEKFPDHAIIAAGSFVFLRFFCPALVSPDSENIIDISHLSEKRTFISLAKVIQNIANGSENFSRWPALCSQKDFLKECSDRIFRFLAELCRTDRTIDIQVRTDPTPIAFDYQFLHSFVYLYGLEVRRNVLNEAKHDDGDIDGDDFYKTTFLLIDDVLGQLGQPKMEFSNEIPIYIREHMDDYPELYEFMNRHAFRNIETSTAYSPSVHESTSSEGIPIITLTMSNFSDRHVDIDTVAYKFLQIYARIWTTKHCLIIDCTEFDEGGLDMRKFISLVMGLLPEVAPKNCIGCYYFNVNETFMDNYGKCLDKDNVYVSSKIPHYFINSNSDEGLMKSVGITGQGLKVLQDIRVSLHDITLYDEKRNRFTPVSLKIGDIYFQVLHETPRQYKIRDMGTLFDVKFNDVYEISRIFEVHVSSITGVAAEFTVTFQDERRLIFSSPKYLEIVKMFYYAQIRLESEYEMDNNSSTSSPNSNNKDKQQKERTKLLCHLLLVSLIGLFDESKKMKNSSYNLIAATEASFGLNFGSHFHRSPEVYVPEDTTTFLGVIGKSLAESNPELTAYMFIYVLEALKNNVIPHVYIPHTICGLSYWIPNLYQHVYLADDEEGPENISHIFRILIRLSVRETDFKAVYMQYVWLLLLDDGRLTDIIVDEVINHALERDSENRDWKKTISLLTVLPTTEVANNIIQKILAKIRSFLPSLKLEAMTQSWSELTILVKISIHVFFETSLLVQMYLPEILFIVSLLIDVGPRELRSSLHQLLMNVCHSLAINSALPQDHRNNLDEISDIFAHQKVKFMFGFSEDKGRILQIFSASSFASKFNILDFFINNILLLMEYSSTYEANVWKTRYKKYVLESVFTSNSFLSARSIMIVGIMGKSYITEGLCKAMLIETMKVIAEPKITDEHLFLAISHIFTYSKIVEGLDPNLDLMKHLFWFSTLFLESRHPIIFEGALLFVSNCIRRLYMAQFENESETSLISTLLKGRKFAHTFLSKIENLSGIVWNEDNFTHILIFIINKGLSNPFIKSTAFDFLKMMFRNSYFEHQINQKSDHYLCYMFLLYFVLNCNQFEELLGDVDFEGEMVNIENKNTIPKILLEWLSSDNENANITLYQGAILFKCSVTDEPSRFRFALIIRHLLTKKPICALRFYSVIRNEIRKISAFEQNSDCVPLAFDILNLLVTHSESNSLEKLHEESIERLTKRGLSIVTSSGIFAKNSDMMIPLDVKPEDIYERKRIMTMILSRMSCSA | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 2 | Phosphorylation | ------MSQPTKNKK ------CCCCCCCCC | 57.05 | 28889911 | |
| 156 | Phosphorylation | SILPDYSSTHSNGNK HCCCCCCCCCCCCCC | 24.74 | 21551504 | |
| 157 | Phosphorylation | ILPDYSSTHSNGNKH CCCCCCCCCCCCCCC | 24.31 | 21551504 | |
| 159 | Phosphorylation | PDYSSTHSNGNKHFF CCCCCCCCCCCCCCC | 46.66 | 21551504 | |
| 224 | Phosphorylation | SILPRHKSYLTRHNH CCCHHCHHHHHHCCC | 21.17 | 15665377 | |
| 263 | Ubiquitination | EYFEFIRKSVQVPVT HHHHHHHHHCCCCCC | 50.60 | 17644757 | |
| 427 | Phosphorylation | NQNIIAKSLSRQLSH CCHHHHHHHHHHHHH | 23.66 | 24961812 | |
| 429 | Phosphorylation | NIIAKSLSRQLSHHQ HHHHHHHHHHHHHHH | 25.68 | 24961812 | |
| 433 | Phosphorylation | KSLSRQLSHHQSYIQ HHHHHHHHHHHHHHH | 15.58 | 23749301 | |
| 437 | Phosphorylation | RQLSHHQSYIQQQSE HHHHHHHHHHHHHHH | 21.61 | 24961812 | |
| 438 | Phosphorylation | QLSHHQSYIQQQSER HHHHHHHHHHHHHHH | 9.00 | 21440633 | |
| 519 | Phosphorylation | QLQQRLNSAAAAAAA HHHHHHHHHHHHHHH | 24.71 | 29136822 | |
| 529 | Phosphorylation | AAAAAAASPSNSTPT HHHHHHHCCCCCCCC | 24.92 | 29136822 | |
| 531 | Phosphorylation | AAAAASPSNSTPTGY HHHHHCCCCCCCCCC | 40.70 | 29136822 | |
| 533 | Phosphorylation | AAASPSNSTPTGYTA HHHCCCCCCCCCCCH | 39.85 | 30377154 | |
| 534 | Phosphorylation | AASPSNSTPTGYTAE HHCCCCCCCCCCCHH | 29.17 | 19779198 | |
| 536 | Phosphorylation | SPSNSTPTGYTAEQQ CCCCCCCCCCCHHHH | 42.80 | 29136822 | |
| 538 | Phosphorylation | SNSTPTGYTAEQQSR CCCCCCCCCHHHHHH | 12.78 | 19779198 | |
| 539 | Phosphorylation | NSTPTGYTAEQQSRA CCCCCCCCHHHHHHH | 25.68 | 19779198 | |
| 544 | Phosphorylation | GYTAEQQSRASYDAH CCCHHHHHHHCCCCC | 29.52 | 19779198 | |
| 547 | Phosphorylation | AEQQSRASYDAHKTG HHHHHHHCCCCCCCC | 23.57 | 19779198 | |
| 629 | Phosphorylation | MHRPKERTSSFNNTA CCCCCCCCCCCCCCC | 29.79 | 29136822 | |
| 630 | Phosphorylation | HRPKERTSSFNNTAH CCCCCCCCCCCCCCC | 38.87 | 29136822 | |
| 631 | Phosphorylation | RPKERTSSFNNTAHE CCCCCCCCCCCCCCC | 31.23 | 19823750 | |
| 635 | Phosphorylation | RTSSFNNTAHETGSE CCCCCCCCCCCCCCC | 30.42 | 29136822 | |
| 639 | Phosphorylation | FNNTAHETGSEKTSG CCCCCCCCCCCCCCC | 35.82 | 19823750 | |
| 641 | Phosphorylation | NTAHETGSEKTSGIK CCCCCCCCCCCCCHH | 43.25 | 19823750 | |
| 655 | Acetylation | KHITQGLKKLTSLPS HHHHHHHHHHHCCCC | 53.19 | 25381059 | |
| 870 | Phosphorylation | TKKHFKGSSPSLCST CCCCCCCCCCCCCCC | 39.40 | 22369663 | |
| 871 | Phosphorylation | KKHFKGSSPSLCSTT CCCCCCCCCCCCCCC | 27.46 | 22369663 | |
| 873 | Phosphorylation | HFKGSSPSLCSTTRS CCCCCCCCCCCCCCC | 43.91 | 22369663 | |
| 880 | Phosphorylation | SLCSTTRSRSGSTSQ CCCCCCCCCCCCCCC | 28.80 | 19779198 | |
| 885 | Phosphorylation | TRSRSGSTSQSSMTP CCCCCCCCCCCCCCC | 34.02 | 19779198 | |
| 891 | Phosphorylation | STSQSSMTPVSPLGL CCCCCCCCCCCCCCC | 23.61 | 27738172 | |
| 919 | Phosphorylation | GSSTSRNSDNVNSLN CCCCCCCCCCCHHCC | 29.47 | 22369663 | |
| 924 | Phosphorylation | RNSDNVNSLNSSPKN CCCCCCHHCCCCCCC | 25.33 | 22369663 | |
| 927 | Phosphorylation | DNVNSLNSSPKNLSS CCCHHCCCCCCCCCC | 54.00 | 22369663 | |
| 928 | Phosphorylation | NVNSLNSSPKNLSSD CCHHCCCCCCCCCCC | 38.53 | 22369663 | |
| 933 | Phosphorylation | NSSPKNLSSDPYLSH CCCCCCCCCCCCHHH | 41.93 | 22369663 | |
| 934 | Phosphorylation | SSPKNLSSDPYLSHL CCCCCCCCCCCHHHH | 45.85 | 22369663 | |
| 937 | Phosphorylation | KNLSSDPYLSHLVAP CCCCCCCCHHHHHHH | 26.31 | 22369663 | |
| 939 | Phosphorylation | LSSDPYLSHLVAPRA CCCCCCHHHHHHHHH | 14.66 | 22369663 | |
| 960 | Ubiquitination | PSSIIRNKIPTTLTS CCHHHHCCCCCCCCC | 39.97 | 17644757 | |
| 963 | Phosphorylation | IIRNKIPTTLTSPPG HHHCCCCCCCCCCCC | 37.60 | 22369663 | |
| 964 | Phosphorylation | IRNKIPTTLTSPPGT HHCCCCCCCCCCCCC | 23.76 | 25521595 | |
| 966 | Phosphorylation | NKIPTTLTSPPGTEK CCCCCCCCCCCCCCC | 36.78 | 22369663 | |
| 967 | Phosphorylation | KIPTTLTSPPGTEKS CCCCCCCCCCCCCCC | 32.01 | 20377248 | |
| 971 | Phosphorylation | TLTSPPGTEKSSPVQ CCCCCCCCCCCCCCC | 46.11 | 20377248 | |
| 973 | Ubiquitination | TSPPGTEKSSPVQRP CCCCCCCCCCCCCCC | 56.95 | 17644757 | |
| 974 | Phosphorylation | SPPGTEKSSPVQRPQ CCCCCCCCCCCCCCC | 33.37 | 20377248 | |
| 975 | Phosphorylation | PPGTEKSSPVQRPQT CCCCCCCCCCCCCCC | 39.91 | 21440633 | |
| 982 | Phosphorylation | SPVQRPQTESISATP CCCCCCCCCCCCCCC | 34.09 | 21440633 | |
| 984 | Phosphorylation | VQRPQTESISATPMA CCCCCCCCCCCCCCE | 25.82 | 21440633 | |
| 986 | Phosphorylation | RPQTESISATPMAIT CCCCCCCCCCCCEEE | 35.06 | 22369663 | |
| 988 | Phosphorylation | QTESISATPMAITNS CCCCCCCCCCEEECC | 13.55 | 22369663 | |
| 993 | Phosphorylation | SATPMAITNSTPLSS CCCCCEEECCCCCHH | 17.36 | 22369663 | |
| 995 | Phosphorylation | TPMAITNSTPLSSAA CCCEEECCCCCHHHC | 23.81 | 22369663 | |
| 996 | Phosphorylation | PMAITNSTPLSSAAF CCEEECCCCCHHHCC | 30.25 | 20377248 | |
| 999 | Phosphorylation | ITNSTPLSSAAFGIR EECCCCCHHHCCCCC | 21.09 | 21440633 | |
| 1000 | Phosphorylation | TNSTPLSSAAFGIRS ECCCCCHHHCCCCCC | 31.16 | 20377248 | |
| 1007 | Phosphorylation | SAAFGIRSPLQKIRT HHCCCCCCHHHHHHH | 28.21 | 28889911 | |
| 1014 | Phosphorylation | SPLQKIRTRRYSDES CHHHHHHHCCCCHHH | 23.99 | 24961812 | |
| 1017 | Phosphorylation | QKIRTRRYSDESLGK HHHHHCCCCHHHHHH | 19.72 | 29136822 | |
| 1018 | Phosphorylation | KIRTRRYSDESLGKF HHHHCCCCHHHHHHH | 32.84 | 21551504 | |
| 1021 | Phosphorylation | TRRYSDESLGKFMKS HCCCCHHHHHHHHHH | 47.62 | 28889911 | |
| 1028 | Phosphorylation | SLGKFMKSTNNYIQE HHHHHHHHCCHHHHH | 25.28 | 19779198 | |
| 1029 | Phosphorylation | LGKFMKSTNNYIQEH HHHHHHHCCHHHHHH | 23.20 | 19779198 | |
| 1173 | Phosphorylation | VKFMKVRSHLAGIAE HHHHHHHHHHHHHHH | 26.32 | 30377154 | |
| 1189 | Phosphorylation | SHHMEYISDSEKLTF HHHHHHCCCHHHCHH | 33.89 | 30377154 | |
| 1191 | Phosphorylation | HMEYISDSEKLTFPL HHHHCCCHHHCHHHH | 29.82 | 30377154 | |
| 1297 | Phosphorylation | LDSMRMIYKKWHTYT HHHHHHHHHHHHHHC | 9.36 | 21126336 | |
| 1342 | Phosphorylation | KKILQEMYPYLLDTV HHHHHHHHHHHHHHH | 6.48 | 30377154 | |
| 1344 | Phosphorylation | ILQEMYPYLLDTVSE HHHHHHHHHHHHHHH | 11.47 | 30377154 | |
| 1348 | Phosphorylation | MYPYLLDTVSELKKN HHHHHHHHHHHHHHH | 26.38 | 30377154 | |
| 1482 | Phosphorylation | IQMTKMFSALQHSEV HHHHHHHHHHCCCCC | 25.27 | 19823750 | |
| 1487 | Phosphorylation | MFSALQHSEVNLGVK HHHHHCCCCCCCCCC | 30.22 | 19823750 | |
| 1560 | Phosphorylation | IAYLTRHTFLEIPPA HHHHHHCCCCCCCCC | 26.99 | 27017623 | |
| 1663 | Phosphorylation | NIVTNYRTYTAKTDL HHHHHCCCCCCCCCH | 18.18 | 30377154 | |
| 1664 | Phosphorylation | IVTNYRTYTAKTDLG HHHHCCCCCCCCCHH | 8.83 | 30377154 | |
| 1665 | Phosphorylation | VTNYRTYTAKTDLGK HHHCCCCCCCCCHHH | 22.51 | 30377154 | |
| 1734 | Phosphorylation | IKNEIEKSSRPTDIL HHHHHHHCCCCCHHH | 20.84 | 30377154 | |
| 1810 | Phosphorylation | YMNELLESISNSVSY HHHHHHHHHHHHHHC | 31.14 | 21551504 | |
| 1817 | Phosphorylation | SISNSVSYFPPPLFY HHHHHHHCCCCCHHH | 20.25 | 21551504 | |
| 2292 | Phosphorylation | QIRLESEYEMDNNSS HHEEEEEEECCCCCC | 26.32 | 28889911 | |
| 2300 | Phosphorylation | EMDNNSSTSSPNSNN ECCCCCCCCCCCCCC | 32.80 | 28889911 | |
| 2316 | Phosphorylation | DKQQKERTKLLCHLL HHHHHHHHHHHHHHH | 27.43 | 28889911 | |
| 3073 | Phosphorylation | RIMTMILSRMSCSA- HHHHHHHHHHCCCC- | 18.27 | 27017623 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of IRA2_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of IRA2_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of IRA2_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-631; SER-871; SER-928;SER-934; SER-995; THR-996 AND SER-1018, AND MASS SPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-934 AND SER-1018, ANDMASS SPECTROMETRY. | |
| "Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry."; Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1018, AND MASSSPECTROMETRY. | |
| "Quantitative phosphoproteomics applied to the yeast pheromonesignaling pathway."; Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J.,Mann M., Jensen O.N.; Mol. Cell. Proteomics 4:310-327(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-224, AND MASSSPECTROMETRY. | |
| "Phosphoproteome analysis by mass spectrometry and its application toSaccharomyces cerevisiae."; Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M.,Shabanowitz J., Hunt D.F., White F.M.; Nat. Biotechnol. 20:301-305(2002). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-924 AND SER-928, ANDMASS SPECTROMETRY. | |
