| UniProt ID | SWA2_YEAST | |
|---|---|---|
| UniProt AC | Q06677 | |
| Protein Name | Auxilin-like clathrin uncoating factor SWA2 | |
| Gene Name | SWA2 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 668 | |
| Subcellular Localization |
Cytoplasm . Endoplasmic reticulum membrane Peripheral membrane protein . |
|
| Protein Description | Cofactor for the uncoating of clathrin-coated vesicles (CCVs) by Hsp70-type chaperones (SSA1/2/3 and SSB1/2). Coat disassembly is important for fusion of vesicles with target membranes and for recycling components of clathrin coats to the cytoplasm for further rounds of vesicle formation. Binds to assembled clathrin and recruits the ATP-activated chaperone to CCVs. Stimulates the ATPase activity of the clathrin-associated Hsp70-type chaperone SSA1, which then disrupts clathrin-clathrin interactions, leading to release of the clathrin coat. In addition, prevents unproductive clathrin assembly in the cell. Also required for cortical endoplasmic reticulum inheritance.. | |
| Protein Sequence | MSDPFAHLLTSLKNKDSASASKETTPQSSNSPSITGSAVADVARTDKSPNDSLHSISAPPLIPSPKVDFSAPPLVPTNSTTKSNTANNTPPSALANTDDDFNQLFGMGTVTTTDTIQKPDEDYYGSKEDHLYNGDDALVDEVKDMEIARLMSLGLSIEEATEFYENDVTYERYLEILKSKQKERNDLAIRKKESGIKMEKSGLSNIVGTDSNNLFSMATDFFNKGKKLVDQWTSFPPEANDRLNNYSKTHDKVEDYDLPQVNDSPNRILFEDNEVVENLPPADNPDQDLLTDFETKIDITKRTAPDVSHSSSPTSGILIEENSRRNEPLIEDSLLDFSEGNLTNSKSNEDSTLFNENSNTDSTIPISDIELSGYNEFKAKGTSLFKNGDYINSLQEYEKSLNTLPLNHPLRIIALSNIIASQLKIGEYSKSIENSSMALELFPSSKAKWKNKISNSDPERSFNDIWPKIMIRRAESFEHLESFKKALETYQELIKKNFFDDKIMQGKRRCQDFINPPPVKKSMPVKKKTTTTSPATKKQNLTASSSNSPISVDSTSEIKKRELENAKLALYDKVFEKISSWKDGKDDDIRHLLANLSSLLTWCNWKDVSMQDLVMPKRVKITYMKAVAKTHPDKIPESLSLENKMIAENIFSTLSIAWDKFKLQNDIN | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 2 | Phosphorylation | ------MSDPFAHLL ------CCCHHHHHH | 54.73 | 22369663 | |
| 17 | Phosphorylation | TSLKNKDSASASKET HHHCCCCCCCCCCCC | 26.23 | 29688323 | |
| 19 | Phosphorylation | LKNKDSASASKETTP HCCCCCCCCCCCCCC | 36.71 | 29688323 | |
| 21 | Phosphorylation | NKDSASASKETTPQS CCCCCCCCCCCCCCC | 28.64 | 29688323 | |
| 24 | Phosphorylation | SASASKETTPQSSNS CCCCCCCCCCCCCCC | 47.73 | 19779198 | |
| 25 | Phosphorylation | ASASKETTPQSSNSP CCCCCCCCCCCCCCC | 22.03 | 21551504 | |
| 28 | Phosphorylation | SKETTPQSSNSPSIT CCCCCCCCCCCCCCC | 32.46 | 21440633 | |
| 29 | Phosphorylation | KETTPQSSNSPSITG CCCCCCCCCCCCCCC | 35.76 | 25752575 | |
| 31 | Phosphorylation | TTPQSSNSPSITGSA CCCCCCCCCCCCCCH | 23.21 | 25752575 | |
| 33 | Phosphorylation | PQSSNSPSITGSAVA CCCCCCCCCCCCHHH | 32.38 | 23749301 | |
| 35 | Phosphorylation | SSNSPSITGSAVADV CCCCCCCCCCHHHHH | 29.44 | 24961812 | |
| 37 | Phosphorylation | NSPSITGSAVADVAR CCCCCCCCHHHHHHC | 15.77 | 21551504 | |
| 45 | Phosphorylation | AVADVARTDKSPNDS HHHHHHCCCCCCCCC | 37.73 | 23749301 | |
| 48 | Phosphorylation | DVARTDKSPNDSLHS HHHCCCCCCCCCCCC | 31.57 | 23749301 | |
| 52 | Phosphorylation | TDKSPNDSLHSISAP CCCCCCCCCCCCCCC | 34.32 | 21440633 | |
| 55 | Phosphorylation | SPNDSLHSISAPPLI CCCCCCCCCCCCCCC | 24.43 | 21440633 | |
| 57 | Phosphorylation | NDSLHSISAPPLIPS CCCCCCCCCCCCCCC | 37.42 | 21440633 | |
| 64 | Phosphorylation | SAPPLIPSPKVDFSA CCCCCCCCCCCCCCC | 30.29 | 17330950 | |
| 70 | Phosphorylation | PSPKVDFSAPPLVPT CCCCCCCCCCCCCCC | 35.09 | 20377248 | |
| 77 | Phosphorylation | SAPPLVPTNSTTKSN CCCCCCCCCCCCCCC | 34.03 | 19823750 | |
| 79 | Phosphorylation | PPLVPTNSTTKSNTA CCCCCCCCCCCCCCC | 39.67 | 24909858 | |
| 80 | Phosphorylation | PLVPTNSTTKSNTAN CCCCCCCCCCCCCCC | 40.07 | 19823750 | |
| 81 | Phosphorylation | LVPTNSTTKSNTANN CCCCCCCCCCCCCCC | 32.09 | 24909858 | |
| 178 | Acetylation | ERYLEILKSKQKERN HHHHHHHHHHHHHHC | 62.36 | 24489116 | |
| 194 | Phosphorylation | LAIRKKESGIKMEKS HHHHHHHHCCCCCCC | 55.48 | 28889911 | |
| 200 | Ubiquitination | ESGIKMEKSGLSNIV HHCCCCCCCCCCCCC | 46.45 | 23749301 | |
| 248 | Ubiquitination | DRLNNYSKTHDKVED HHHHCCCCCCCCCCC | 39.25 | 22817900 | |
| 252 | Ubiquitination | NYSKTHDKVEDYDLP CCCCCCCCCCCCCCC | 39.66 | 23749301 | |
| 252 | Acetylation | NYSKTHDKVEDYDLP CCCCCCCCCCCCCCC | 39.66 | 24489116 | |
| 256 | Phosphorylation | THDKVEDYDLPQVND CCCCCCCCCCCCCCC | 13.08 | 28132839 | |
| 264 | Phosphorylation | DLPQVNDSPNRILFE CCCCCCCCCCEEEEC | 20.90 | 22369663 | |
| 303 | Phosphorylation | KIDITKRTAPDVSHS HCCCCCCCCCCCCCC | 44.06 | 22369663 | |
| 308 | Phosphorylation | KRTAPDVSHSSSPTS CCCCCCCCCCCCCCC | 25.65 | 22369663 | |
| 310 | Phosphorylation | TAPDVSHSSSPTSGI CCCCCCCCCCCCCCE | 25.63 | 22369663 | |
| 311 | Phosphorylation | APDVSHSSSPTSGIL CCCCCCCCCCCCCEE | 34.61 | 22369663 | |
| 312 | Phosphorylation | PDVSHSSSPTSGILI CCCCCCCCCCCCEEE | 34.90 | 22369663 | |
| 314 | Phosphorylation | VSHSSSPTSGILIEE CCCCCCCCCCEEECC | 41.34 | 22369663 | |
| 315 | Phosphorylation | SHSSSPTSGILIEEN CCCCCCCCCEEECCC | 27.50 | 22369663 | |
| 323 | Phosphorylation | GILIEENSRRNEPLI CEEECCCCCCCCCCC | 35.58 | 22369663 | |
| 333 | Phosphorylation | NEPLIEDSLLDFSEG CCCCCCCHHHCCCCC | 20.31 | 24961812 | |
| 338 | Phosphorylation | EDSLLDFSEGNLTNS CCHHHCCCCCCCCCC | 44.24 | 28889911 | |
| 382 | Phosphorylation | NEFKAKGTSLFKNGD CCCCCCCCCHHCCCC | 22.88 | 21440633 | |
| 383 | Phosphorylation | EFKAKGTSLFKNGDY CCCCCCCCHHCCCCH | 40.24 | 21440633 | |
| 386 | Ubiquitination | AKGTSLFKNGDYINS CCCCCHHCCCCHHHH | 66.37 | 23749301 | |
| 424 | Ubiquitination | NIIASQLKIGEYSKS HHHHHHHHHCCCCHH | 40.82 | 17644757 | |
| 476 | Phosphorylation | IMIRRAESFEHLESF HHHHHHHHHHHHHHH | 34.64 | 28889911 | |
| 531 | Phosphorylation | PVKKKTTTTSPATKK CCCCCCCCCCCCCCC | 30.43 | 30377154 | |
| 532 | Phosphorylation | VKKKTTTTSPATKKQ CCCCCCCCCCCCCCC | 30.53 | 30377154 | |
| 533 | Phosphorylation | KKKTTTTSPATKKQN CCCCCCCCCCCCCCC | 15.50 | 28889911 | |
| 536 | Phosphorylation | TTTTSPATKKQNLTA CCCCCCCCCCCCCCC | 42.00 | 30377154 | |
| 542 | Phosphorylation | ATKKQNLTASSSNSP CCCCCCCCCCCCCCC | 31.85 | 22369663 | |
| 544 | Phosphorylation | KKQNLTASSSNSPIS CCCCCCCCCCCCCCC | 29.12 | 22369663 | |
| 545 | Phosphorylation | KQNLTASSSNSPISV CCCCCCCCCCCCCCC | 30.95 | 22369663 | |
| 546 | Phosphorylation | QNLTASSSNSPISVD CCCCCCCCCCCCCCC | 38.54 | 22369663 | |
| 548 | Phosphorylation | LTASSSNSPISVDST CCCCCCCCCCCCCCC | 26.09 | 22369663 | |
| 551 | Phosphorylation | SSSNSPISVDSTSEI CCCCCCCCCCCCCHH | 24.21 | 22369663 | |
| 554 | Phosphorylation | NSPISVDSTSEIKKR CCCCCCCCCCHHHHH | 31.51 | 19823750 | |
| 555 | Phosphorylation | SPISVDSTSEIKKRE CCCCCCCCCHHHHHH | 26.39 | 19823750 | |
| 556 | Phosphorylation | PISVDSTSEIKKREL CCCCCCCCHHHHHHH | 41.08 | 19823750 | |
| 634 | Acetylation | VAKTHPDKIPESLSL HHHHCCCCCCCCCCC | 65.69 | 24489116 | |
| 638 | Phosphorylation | HPDKIPESLSLENKM CCCCCCCCCCCCCHH | 20.22 | 28152593 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of SWA2_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of SWA2_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of SWA2_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48; SER-64; SER-79;SER-264; SER-311; SER-312; THR-314; SER-315 AND SER-338, AND MASSSPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64; SER-310; SER-311;SER-312 AND THR-314, AND MASS SPECTROMETRY. | |
| "Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae."; Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.; J. Proteome Res. 6:1190-1197(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64; SER-264; SER-308 ANDSER-312, AND MASS SPECTROMETRY. | |
