| UniProt ID | IPL1_YEAST | |
|---|---|---|
| UniProt AC | P38991 | |
| Protein Name | Spindle assembly checkpoint kinase | |
| Gene Name | IPL1 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 367 | |
| Subcellular Localization | Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Associates with the mitotic spindle and on elongated and disassembling spindles. Also associated with the kinetochore. | |
| Protein Description | Required for high-fidelity chromosome segregation during the later part of each cell cycle. Acts in opposition to the phosphatase PP1. Has a role in attaching the kinetochores to the microtubules and ensuring that sister kinetochores connect to opposite poles. The promotion of bi-orientation is achieved by selectively detaching kinetochore-microtubule attachments that are not under tension. Phosphorylates histone H3 to form H3S10ph during mitosis and meiosis. Phosphorylates RGD1 in vitro.. | |
| Protein Sequence | MQRNSLVNIKLNANSPSKKTTTRPNTSRINKPWRISHSPQQRNPNSKIPSPVREKLNRLPVNNKKFLDMESSKIPSPIRKATSSKMIHENKKLPKFKSLSLDDFELGKKLGKGKFGKVYCVRHRSTGYICALKVMEKEEIIKYNLQKQFRREVEIQTSLNHPNLTKSYGYFHDEKRVYLLMEYLVNGEMYKLLRLHGPFNDILASDYIYQIANALDYMHKKNIIHRDIKPENILIGFNNVIKLTDFGWSIINPPENRRKTVCGTIDYLSPEMVESREYDHTIDAWALGVLAFELLTGAPPFEEEMKDTTYKRIAALDIKMPSNISQDAQDLILKLLKYDPKDRMRLGDVKMHPWILRNKPFWENKRL | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 5 | Phosphorylation | ---MQRNSLVNIKLN ---CCCCCCEEEEEC | 35.56 | 19823750 | |
| 15 | Phosphorylation | NIKLNANSPSKKTTT EEEECCCCCCCCCCC | 28.30 | 28889911 | |
| 36 | Phosphorylation | INKPWRISHSPQQRN CCCCCCCCCCCCCCC | 14.89 | 17287358 | |
| 38 | Phosphorylation | KPWRISHSPQQRNPN CCCCCCCCCCCCCCC | 20.01 | 17287358 | |
| 50 | Phosphorylation | NPNSKIPSPVREKLN CCCCCCCCHHHHHHH | 39.23 | 25521595 | |
| 71 | Phosphorylation | KKFLDMESSKIPSPI CCCCCCCCCCCCCCH | 30.23 | 24961812 | |
| 72 | Phosphorylation | KFLDMESSKIPSPIR CCCCCCCCCCCCCHH | 22.69 | 21440633 | |
| 76 | Phosphorylation | MESSKIPSPIRKATS CCCCCCCCCHHHHHH | 36.11 | 21551504 | |
| 178 | Phosphorylation | FHDEKRVYLLMEYLV ECCHHHHHHEEHHHH | 9.73 | 28132839 | |
| 183 | Phosphorylation | RVYLLMEYLVNGEMY HHHHEEHHHHCCHHH | 11.28 | 28132839 | |
| 190 | Phosphorylation | YLVNGEMYKLLRLHG HHHCCHHHHHHHHHC | 8.32 | 28132839 | |
| 260 | Phosphorylation | PPENRRKTVCGTIDY CCCCCCCCCEEECCC | 21.15 | 8007975 | |
| 264 | Phosphorylation | RRKTVCGTIDYLSPE CCCCCEEECCCCCHH | 12.92 | 27214570 | |
| 322 | Phosphorylation | ALDIKMPSNISQDAQ ECCCCCCCCCCHHHH | 43.21 | 27017623 | |
| 325 | Phosphorylation | IKMPSNISQDAQDLI CCCCCCCCHHHHHHH | 26.68 | 27017623 |
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of IPL1_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of IPL1_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50 AND SER-76, AND MASSSPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, AND MASSSPECTROMETRY. | |
| "Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry."; Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36 AND SER-38, AND MASSSPECTROMETRY. | |
| "Phospho-regulation of kinetochore-microtubule attachments by theAurora kinase Ipl1p."; Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S.,Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.; Cell 111:163-172(2002). Cited for: FUNCTION, AND PHOSPHORYLATION AT SER-5; SER-76 AND THR-260. | |
