YNF8_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: YNF8_YEAST
• UniProt AC: P53947
• Protein Name: Vacuolar membrane protein YNL058C
• Gene Name: YNL058C
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 316
• Subcellular Localization: Vacuole membrane ; Single-pass membrane protein .
• Protein Sequence: MVKKNFIPSVSLVRRDLPTLVTTTTSSTALSKPTSSVVSETSSKSLPSLTSSAFSTSSGATSSSSLIVASITPPSTAGNPFILNAADKPNGTVYIAVGAVIGAIFISILIWWLVSSYLSRRFTMTNSYANDSKNLYRGHHKHSSSLQSNPFDINDEKSYMQDDWDSMSQLESSQYEDAASPFNPIQDPFTDNRRSLFISPTLQVSQYEKSHSRHQSKDTNIFIDDPFLYVGTYLEEEEEEEEERKLNLNRPQRAASPERKEKKINSMEGYHKRNQSSLGLIPVASATSNTSSPKKAHKRQAPSMFLDDVLNGREII

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
19	Phosphorylation	LVRRDLPTLVTTTTS HHCCCCCEEEEEECC	40.95	27017623
25	Phosphorylation	PTLVTTTTSSTALSK CEEEEEECCCCCCCC	20.70	27017623
31	Phosphorylation	TTSSTALSKPTSSVV ECCCCCCCCCCCCCC	33.84	30377154
36	Phosphorylation	ALSKPTSSVVSETSS CCCCCCCCCCCCCCC	29.08	27017623
133	Ubiquitination	NSYANDSKNLYRGHH CCCCCCCCCCCCCCC	54.56	23749301
143	Phosphorylation	YRGHHKHSSSLQSNP CCCCCCCCCCCCCCC	26.82	19779198
144	Phosphorylation	RGHHKHSSSLQSNPF CCCCCCCCCCCCCCC	34.48	23749301
145	Phosphorylation	GHHKHSSSLQSNPFD CCCCCCCCCCCCCCC	33.18	21440633
148	Phosphorylation	KHSSSLQSNPFDIND CCCCCCCCCCCCCCC	52.33	19823750
195	Phosphorylation	PFTDNRRSLFISPTL CCCCCCCCEEECCCE	25.13	29650682
199	Phosphorylation	NRRSLFISPTLQVSQ CCCCEEECCCEECHH	12.42	27017623
209	Acetylation	LQVSQYEKSHSRHQS EECHHHHHHHCCCCC	48.84	24489116
256	Phosphorylation	NRPQRAASPERKEKK CCHHHCCCHHHHHHH	26.72	17287358
266	Phosphorylation	RKEKKINSMEGYHKR HHHHHHHHHCCHHHC	22.99	21440633
270	Phosphorylation	KINSMEGYHKRNQSS HHHHHCCHHHCCHHC	7.29	27017623
276	Phosphorylation	GYHKRNQSSLGLIPV CHHHCCHHCCCCEEE	30.95	19823750
277	Phosphorylation	YHKRNQSSLGLIPVA HHHCCHHCCCCEEEC	19.38	20377248
285	Phosphorylation	LGLIPVASATSNTSS CCCEEECCCCCCCCC	32.11	20377248
287	Phosphorylation	LIPVASATSNTSSPK CEEECCCCCCCCCCC	21.73	19684113
288	Phosphorylation	IPVASATSNTSSPKK EEECCCCCCCCCCCH	37.52	19684113
290	Phosphorylation	VASATSNTSSPKKAH ECCCCCCCCCCCHHH	30.28	20377248
291	Phosphorylation	ASATSNTSSPKKAHK CCCCCCCCCCCHHHH	49.22	19684113
292	Phosphorylation	SATSNTSSPKKAHKR CCCCCCCCCCHHHHC	38.41	19823750
303	Phosphorylation	AHKRQAPSMFLDDVL HHHCCCCCCCHHHHH	25.78	21440633

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of YNF8_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of YNF8_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of YNF8_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EIF3J_YEAST	HCR1	genetic	27708008
LTE1_YEAST	LTE1	genetic	27708008
REI1_YEAST	REI1	genetic	27708008
PDE2_YEAST	PDE2	genetic	27708008

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases.";
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292, AND MASSSPECTROMETRY.
PubMed: 17563356

"Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry.";
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-256, AND MASSSPECTROMETRY.
PubMed: 17287358