| UniProt ID | VAC7_YEAST | |
|---|---|---|
| UniProt AC | P53950 | |
| Protein Name | Vacuolar segregation protein 7 | |
| Gene Name | VAC7 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 1165 | |
| Subcellular Localization |
Vacuole membrane Single-pass type II membrane protein . |
|
| Protein Description | The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Positively regulates FAB1 kinase activity. Major activator of FAB1 during hyperosmotic shock and can elevate levels of PtdIns(3,5)P2 in the absence of VAC14 and FIG4. Directly involved in vacuolar membrane scission. Required for normal vacuole acidification, inheritance and morphology.. | |
| Protein Sequence | MTEEDRKLTVETETVEAPVANNLLLSNNSNVVAPNPSIPSASTSTSPLHREIVDDSVATANTTSNVVQHNLPTIDNNLMDSDATSHNQDHWHSDINRAGTSMSTSDIPTDLHLEHIGSVSSTNNNSNNALINHNPLSSHLSNPSSSLRNKKSSLLVASNPAFASDVELSKKKPAVISNNMPTSNIALYQTARSANIHGPSSTSASKAFRKASAFSNNTAPSTSNNIGSNTPPAPLLPLPSLSQQNKPKIIERPTMHVTNSREILLGENLLDDTKAKNAPANSTTHDNGPVANDGLRIPNHSNADDNENNNKMKKNKNINSGKNERNDDTSKICTTSTKTAPSTAPLGSTDNTQALTASVSSSNADNHNNNKKKTSSNNNGNNSNSASNKTNADIKNSNADLSASTSNNNAINDDSHESNSEKPTKADFFAARLATAVGENEISDSEETFVYESAANSTKNLIFPDSSSQQQQQQQQPPKQQQQQQNHGITSKISAPLLNNNKKLLSRLKNSRHISTGAILNNTIATISTNPNLNSNVMQNNNNLMSGHNHLDELSSIKQEPPHQLQQQQPPMDVQSVDSYTSDNPDSNVIAKSPDKRSSLVSLSKVSPHLLSSTSSNGNTISCPNVATNSQELEPNNDISTKKSLSNSTLRHSSANRNSNYGDNKRPLRTTVSKIFDSNPNGAPLRRYSGVPDHVNLEDYIEQPHNYPTMQNSVKKDEFYNSRNNKFPHGLNFYGDNNVIEEENNGDSSNVNRPQHTNLQHEFIPEDNESDENDIHSMFYYNHKNDLETKPLISDYGEDEDVDDYDRPNATFNSYYGSASNTHELPLHGRMPSRSNNDYYDFMVGNNTGNNNQLNEYTPLRMKRGQRHLSRTNNSIMNGSIHMNGNDDVTHSNINNNDIVGYSPHNFYSRKSPFVKVKNFLYLAFVISSLLMTGFILGFLLATNKELQDVDVVVMDNVISSSDELIFDITVSAFNPGFFSISVSQVDLDIFAKSSYLKCDSNGDCTVMEQERKILQITTNLSLVEESANNDISGGNIETVLLGTAKKLETPLKFQGGAFNRNYDVSVSSVKLLSPGSREAKHENDDDDDDDGDDGDDENNTNERQYKSKPNARDDKEDDTKKWKLLIKHDYELIVRGSMKYEVPFFNTQKSTAIQKDSMVHPGKK | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 152 | Phosphorylation | SSLRNKKSSLLVASN HHHCCCCCCEEEECC | 28.03 | 28889911 | |
| 153 | Phosphorylation | SLRNKKSSLLVASNP HHCCCCCCEEEECCH | 34.30 | 21440633 | |
| 164 | Phosphorylation | ASNPAFASDVELSKK ECCHHHHCCCHHHCC | 35.42 | 22369663 | |
| 169 | Phosphorylation | FASDVELSKKKPAVI HHCCCHHHCCCCEEE | 28.99 | 29688323 | |
| 228 | Phosphorylation | STSNNIGSNTPPAPL CCCCCCCCCCCCCCC | 33.46 | 21551504 | |
| 230 | Phosphorylation | SNNIGSNTPPAPLLP CCCCCCCCCCCCCCC | 32.14 | 21440633 | |
| 254 | Phosphorylation | PKIIERPTMHVTNSR CCCCCCCCEECCCCC | 26.57 | 21440633 | |
| 258 | Phosphorylation | ERPTMHVTNSREILL CCCCEECCCCCHHHC | 16.88 | 22369663 | |
| 260 | Phosphorylation | PTMHVTNSREILLGE CCEECCCCCHHHCCC | 23.17 | 20377248 | |
| 273 | Phosphorylation | GENLLDDTKAKNAPA CCCCCCCCCCCCCCC | 32.35 | 22369663 | |
| 352 | Phosphorylation | PLGSTDNTQALTASV CCCCCCCHHHEEEEC | 20.30 | 19779198 | |
| 358 | Phosphorylation | NTQALTASVSSSNAD CHHHEEEECCCCCCC | 19.80 | 23749301 | |
| 361 | Phosphorylation | ALTASVSSSNADNHN HEEEECCCCCCCCCC | 26.00 | 19779198 | |
| 362 | Phosphorylation | LTASVSSSNADNHNN EEEECCCCCCCCCCC | 28.65 | 28889911 | |
| 376 | Phosphorylation | NNKKKTSSNNNGNNS CCCCCCCCCCCCCCC | 49.13 | 27017623 | |
| 385 | Phosphorylation | NNGNNSNSASNKTNA CCCCCCCCCCCCCCH | 32.66 | 23749301 | |
| 387 | Phosphorylation | GNNSNSASNKTNADI CCCCCCCCCCCCHHH | 38.38 | 25371407 | |
| 390 | Phosphorylation | SNSASNKTNADIKNS CCCCCCCCCHHHHCC | 39.63 | 25371407 | |
| 397 | Phosphorylation | TNADIKNSNADLSAS CCHHHHCCCCCCCCC | 28.52 | 23749301 | |
| 402 | Phosphorylation | KNSNADLSASTSNNN HCCCCCCCCCCCCCC | 22.26 | 24961812 | |
| 404 | Phosphorylation | SNADLSASTSNNNAI CCCCCCCCCCCCCCC | 29.06 | 24961812 | |
| 405 | Phosphorylation | NADLSASTSNNNAIN CCCCCCCCCCCCCCC | 34.88 | 24961812 | |
| 406 | Phosphorylation | ADLSASTSNNNAIND CCCCCCCCCCCCCCC | 34.76 | 23749301 | |
| 418 | Phosphorylation | INDDSHESNSEKPTK CCCCCCCCCCCCCCH | 39.99 | 21551504 | |
| 420 | Phosphorylation | DDSHESNSEKPTKAD CCCCCCCCCCCCHHH | 57.17 | 21440633 | |
| 435 | Phosphorylation | FFAARLATAVGENEI HHHHHHHHHHCCCCC | 26.85 | 22369663 | |
| 443 | Phosphorylation | AVGENEISDSEETFV HHCCCCCCCCCCEEE | 28.98 | 22369663 | |
| 445 | Phosphorylation | GENEISDSEETFVYE CCCCCCCCCCEEEEE | 30.46 | 22369663 | |
| 448 | Phosphorylation | EISDSEETFVYESAA CCCCCCCEEEEECCC | 17.85 | 22369663 | |
| 451 | Phosphorylation | DSEETFVYESAANST CCCCEEEEECCCCCC | 10.44 | 22369663 | |
| 453 | Phosphorylation | EETFVYESAANSTKN CCEEEEECCCCCCCC | 18.26 | 22369663 | |
| 457 | Phosphorylation | VYESAANSTKNLIFP EEECCCCCCCCEECC | 35.71 | 22369663 | |
| 458 | Phosphorylation | YESAANSTKNLIFPD EECCCCCCCCEECCC | 24.61 | 22369663 | |
| 468 | Phosphorylation | LIFPDSSSQQQQQQQ EECCCCHHHHHHHHH | 35.64 | 28889911 | |
| 490 | Phosphorylation | QQQNHGITSKISAPL HHHHCCCCHHCCHHH | 28.67 | 19823750 | |
| 491 | Phosphorylation | QQNHGITSKISAPLL HHHCCCCHHCCHHHH | 26.52 | 24961812 | |
| 494 | Phosphorylation | HGITSKISAPLLNNN CCCCHHCCHHHHCCC | 27.80 | 17563356 | |
| 576 | Phosphorylation | QPPMDVQSVDSYTSD CCCCCHHHCCCCCCC | 27.95 | 19779198 | |
| 579 | Phosphorylation | MDVQSVDSYTSDNPD CCHHHCCCCCCCCCC | 28.31 | 28889911 | |
| 580 | Phosphorylation | DVQSVDSYTSDNPDS CHHHCCCCCCCCCCC | 13.05 | 20377248 | |
| 581 | Phosphorylation | VQSVDSYTSDNPDSN HHHCCCCCCCCCCCC | 33.29 | 20377248 | |
| 582 | Phosphorylation | QSVDSYTSDNPDSNV HHCCCCCCCCCCCCC | 27.45 | 19779198 | |
| 593 | Phosphorylation | DSNVIAKSPDKRSSL CCCCCCCCCCCCHHC | 29.34 | 19795423 | |
| 598 | Phosphorylation | AKSPDKRSSLVSLSK CCCCCCCHHCCHHHH | 33.49 | 22369663 | |
| 599 | Phosphorylation | KSPDKRSSLVSLSKV CCCCCCHHCCHHHHC | 36.50 | 22369663 | |
| 602 | Phosphorylation | DKRSSLVSLSKVSPH CCCHHCCHHHHCCHH | 32.02 | 22369663 | |
| 604 | Phosphorylation | RSSLVSLSKVSPHLL CHHCCHHHHCCHHHH | 24.41 | 22369663 | |
| 607 | Phosphorylation | LVSLSKVSPHLLSST CCHHHHCCHHHHCCC | 15.44 | 28889911 | |
| 612 | Phosphorylation | KVSPHLLSSTSSNGN HCCHHHHCCCCCCCC | 37.62 | 20377248 | |
| 613 | Phosphorylation | VSPHLLSSTSSNGNT CCHHHHCCCCCCCCE | 32.07 | 21551504 | |
| 614 | Phosphorylation | SPHLLSSTSSNGNTI CHHHHCCCCCCCCEE | 32.57 | 21440633 | |
| 615 | Phosphorylation | PHLLSSTSSNGNTIS HHHHCCCCCCCCEEE | 24.61 | 21551504 | |
| 616 | Phosphorylation | HLLSSTSSNGNTISC HHHCCCCCCCCEEEC | 49.17 | 21551504 | |
| 620 | Phosphorylation | STSSNGNTISCPNVA CCCCCCCEEECCCCC | 18.70 | 19779198 | |
| 644 | Phosphorylation | NDISTKKSLSNSTLR CCCCCCHHHCCHHHC | 38.29 | 22369663 | |
| 646 | Phosphorylation | ISTKKSLSNSTLRHS CCCCHHHCCHHHCCC | 35.51 | 22369663 | |
| 648 | Phosphorylation | TKKSLSNSTLRHSSA CCHHHCCHHHCCCCC | 25.99 | 22369663 | |
| 649 | Phosphorylation | KKSLSNSTLRHSSAN CHHHCCHHHCCCCCC | 31.87 | 22369663 | |
| 653 | Phosphorylation | SNSTLRHSSANRNSN CCHHHCCCCCCCCCC | 25.39 | 24961812 | |
| 670 | Phosphorylation | DNKRPLRTTVSKIFD CCCCCCCHHHHHHHC | 38.95 | 21551504 | |
| 673 | Phosphorylation | RPLRTTVSKIFDSNP CCCCHHHHHHHCCCC | 20.24 | 23749301 | |
| 674 | Acetylation | PLRTTVSKIFDSNPN CCCHHHHHHHCCCCC | 42.95 | 24489116 | |
| 689 | Phosphorylation | GAPLRRYSGVPDHVN CCCCCCCCCCCCCCC | 31.35 | 28889911 | |
| 713 | Phosphorylation | NYPTMQNSVKKDEFY CCCCCCHHCCHHHHH | 20.58 | 28889911 | |
| 835 | Phosphorylation | HGRMPSRSNNDYYDF CCCCCCCCCCCCEEE | 44.30 | 27017623 | |
| 839 | Phosphorylation | PSRSNNDYYDFMVGN CCCCCCCCEEEECCC | 13.68 | 27017623 | |
| 840 | Phosphorylation | SRSNNDYYDFMVGNN CCCCCCCEEEECCCC | 12.93 | 27017623 | |
| 848 | Phosphorylation | DFMVGNNTGNNNQLN EEECCCCCCCCCCCC | 45.33 | 27017623 | |
| 857 | Phosphorylation | NNNQLNEYTPLRMKR CCCCCCCCCCCCCHH | 16.75 | 27017623 | |
| 880 | Phosphorylation | NNSIMNGSIHMNGND CCCCCCCCEEECCCC | 12.33 | 19779198 | |
| 892 | Phosphorylation | GNDDVTHSNINNNDI CCCCCCCCCCCCCCE | 30.00 | 19779198 | |
| 945 | Ubiquitination | GFLLATNKELQDVDV HHHHHCCCCCCCCCE | 56.28 | 15699485 | |
| 1020 | N-linked_Glycosylation | KILQITTNLSLVEES HHHHHHHCHHHHHHH | 20.98 | - | |
| 1053 | Acetylation | KKLETPLKFQGGAFN CCCCCCCEECCCCCC | 36.52 | 24489116 | |
| 1099 | N-linked_Glycosylation | GDDGDDENNTNERQY CCCCCCCCCCCHHHH | 68.56 | - |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of VAC7_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of VAC7_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of VAC7_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-258; SER-443; SER-579;SER-602 AND SER-607, AND MASS SPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-494, AND MASSSPECTROMETRY. | |
