ALK1_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ALK1_YEAST
• UniProt AC: P43633
• Protein Name: Serine/threonine-protein kinase Haspin homolog ALK1
• Gene Name: ALK1
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 760
• Protein Description: Serine/threonine haspin-like protein kinase involved in cell cycle regulation..
• Protein Sequence: MLLHFDIVIQLLSSHTLKSHQVEPPMDFETSFEEFVEDKRFIALEVSDNDDDCDTDLTADTADELESSAILKMRESDASLNVTTGNNTSRKTTSNSKKRWSLLSNHSAVSSSKSKKRWSVLSSSFTSESHKDRESRNVLQQKRKSLQSYSSLDTVASNSSISASSSLKRSSTGLSLRQLFTKIGINDDISQPGIGIPQGKENLPPTMGKKNSSIASTSSENRLRTPLKPLVNHSKRPTSQPQQQQPLYNASLSSRRSSISSTVSSSSSSKWRFWKRNKNQTPALLQPDHHSLKTFPAVNRRDSMTPVEPRNMVKHKTSFSDFHKTIFSSNTYSESSDTISSMEITLKNKASSSSLSLNVLKKRNSQSSLKHKSSHASLQKFKRNKGKSSMIAPSTATNSSNDDSCSYSSKNSTLSHRISLPVPDQVSRDKIQNKLRYSTSLLSLNSKSSLPMNKNDHDETLLRQILLNCDIKRILNPAKGDVLPLINDVNHLSSIQLTSNVWQIGEVICKKVSLGTIDDITWDRKFLSLQELEKLKIMQQKFDGIPQLLKSFVVKEANGGLYLYLLFKDHGTPISLISLKNWKQILKIFWSCAGIIHGLEKNLKFEHRNLTLDNILIDGNGNITIIDFKCSRLQTPQDDVLYLRLDHPLFFLNGKDKSKINEYQYQFEFEIYQSMRILLNMDASAFEPMTNLYWLYYLSRVLLKFGDRKLGKNDANRDKMARVINHLEMNLAVHKRGGQLFKRLETEDIKNTGDLLKLYK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
76	Phosphorylation	AILKMRESDASLNVT HHHHHHHHCCCCEEE	28.28	19823750
79	Phosphorylation	KMRESDASLNVTTGN HHHHHCCCCEEECCC	26.01	19823750
83	Phosphorylation	SDASLNVTTGNNTSR HCCCCEEECCCCCCC	28.10	21440633
91	Ubiquitination	TGNNTSRKTTSNSKK CCCCCCCEECCCCHH	56.79	17644757
101	Phosphorylation	SNSKKRWSLLSNHSA CCCHHHHHHHHCCCC	24.38	19823750
104	Phosphorylation	KKRWSLLSNHSAVSS HHHHHHHHCCCCCCC	37.48	23749301
107	Phosphorylation	WSLLSNHSAVSSSKS HHHHHCCCCCCCCCC	34.32	23749301
110	Phosphorylation	LSNHSAVSSSKSKKR HHCCCCCCCCCCHHH	28.72	23749301
111	Phosphorylation	SNHSAVSSSKSKKRW HCCCCCCCCCCHHHH	34.88	19823750
112	Phosphorylation	NHSAVSSSKSKKRWS CCCCCCCCCCHHHHE	33.18	23749301
113	Ubiquitination	HSAVSSSKSKKRWSV CCCCCCCCCHHHHEE	69.14	17644757
122	Phosphorylation	KKRWSVLSSSFTSES HHHHEEECCCCCCHH	23.06	24961812
123	Phosphorylation	KRWSVLSSSFTSESH HHHEEECCCCCCHHH	25.88	24961812
124	Phosphorylation	RWSVLSSSFTSESHK HHEEECCCCCCHHHC	29.21	24961812
126	Phosphorylation	SVLSSSFTSESHKDR EEECCCCCCHHHCCH	33.27	24961812
127	Phosphorylation	VLSSSFTSESHKDRE EECCCCCCHHHCCHH	34.78	24961812
129	Phosphorylation	SSSFTSESHKDRESR CCCCCCHHHCCHHHH	35.29	24961812
142	Acetylation	SRNVLQQKRKSLQSY HHHHHHHHHHHHHCC	49.16	25381059
157	Phosphorylation	SSLDTVASNSSISAS HHHHHHHCCCCCCCC	32.67	19779198
159	Phosphorylation	LDTVASNSSISASSS HHHHHCCCCCCCCHH	26.93	19779198
160	Phosphorylation	DTVASNSSISASSSL HHHHCCCCCCCCHHC	25.47	19779198
162	Phosphorylation	VASNSSISASSSLKR HHCCCCCCCCHHCCC	24.85	19779198
164	Phosphorylation	SNSSISASSSLKRSS CCCCCCCCHHCCCCC	17.42	19779198
165	Phosphorylation	NSSISASSSLKRSST CCCCCCCHHCCCCCC	39.13	19779198
170	Phosphorylation	ASSSLKRSSTGLSLR CCHHCCCCCCCCCHH	30.57	22369663
171	Phosphorylation	SSSLKRSSTGLSLRQ CHHCCCCCCCCCHHH	30.44	22369663
172	Phosphorylation	SSLKRSSTGLSLRQL HHCCCCCCCCCHHHH	43.31	20377248
175	Phosphorylation	KRSSTGLSLRQLFTK CCCCCCCCHHHHHHH	23.89	22369663
181	Phosphorylation	LSLRQLFTKIGINDD CCHHHHHHHHCCCCC	30.28	19823750
182	Ubiquitination	SLRQLFTKIGINDDI CHHHHHHHHCCCCCC	31.44	17644757
190	Phosphorylation	IGINDDISQPGIGIP HCCCCCCCCCCCCCC	37.00	29688323
200	Ubiquitination	GIGIPQGKENLPPTM CCCCCCCCCCCCCCC	37.88	17644757
209	Ubiquitination	NLPPTMGKKNSSIAS CCCCCCCCCCCCCCC	37.65	23749301
210	Ubiquitination	LPPTMGKKNSSIAST CCCCCCCCCCCCCCC	57.23	23749301
212	Phosphorylation	PTMGKKNSSIASTSS CCCCCCCCCCCCCCC	31.81	23749301
213	Phosphorylation	TMGKKNSSIASTSSE CCCCCCCCCCCCCCC	31.41	28889911
216	Phosphorylation	KKNSSIASTSSENRL CCCCCCCCCCCCCCC	27.44	21551504
218	Phosphorylation	NSSIASTSSENRLRT CCCCCCCCCCCCCCC	32.91	23749301
219	Phosphorylation	SSIASTSSENRLRTP CCCCCCCCCCCCCCC	38.69	23749301
228	Ubiquitination	NRLRTPLKPLVNHSK CCCCCCCHHHCCCCC	37.19	17644757
235	Ubiquitination	KPLVNHSKRPTSQPQ HHHCCCCCCCCCCCH	55.64	17644757
251	Phosphorylation	QQPLYNASLSSRRSS CCCCHHHCHHCCCCC	25.75	25704821
253	Phosphorylation	PLYNASLSSRRSSIS CCHHHCHHCCCCCCC	21.21	19779198
254	Phosphorylation	LYNASLSSRRSSISS CHHHCHHCCCCCCCC	36.69	23749301
257	Phosphorylation	ASLSSRRSSISSTVS HCHHCCCCCCCCCCC	30.80	22890988
258	Phosphorylation	SLSSRRSSISSTVSS CHHCCCCCCCCCCCC	25.36	22890988
260	Phosphorylation	SSRRSSISSTVSSSS HCCCCCCCCCCCCCC	23.15	22890988
261	Phosphorylation	SRRSSISSTVSSSSS CCCCCCCCCCCCCCC	31.03	21440633
262	Phosphorylation	RRSSISSTVSSSSSS CCCCCCCCCCCCCCC	20.02	23749301
264	Phosphorylation	SSISSTVSSSSSSKW CCCCCCCCCCCCCCC	25.29	22890988
265	Phosphorylation	SISSTVSSSSSSKWR CCCCCCCCCCCCCCE	29.87	22890988
266	Phosphorylation	ISSTVSSSSSSKWRF CCCCCCCCCCCCCEE	27.08	22890988
267	Phosphorylation	SSTVSSSSSSKWRFW CCCCCCCCCCCCEEE	40.35	22890988
268	Phosphorylation	STVSSSSSSKWRFWK CCCCCCCCCCCEEEE	37.57	22890988
269	Phosphorylation	TVSSSSSSKWRFWKR CCCCCCCCCCEEEEC	37.88	21440633
270	Ubiquitination	VSSSSSSKWRFWKRN CCCCCCCCCEEEECC	44.32	17644757
278	Ubiquitination	WRFWKRNKNQTPALL CEEEECCCCCCCCCC	55.24	17644757
291	Phosphorylation	LLQPDHHSLKTFPAV CCCCCCHHCCCCCCC	28.30	21440633
293	Ubiquitination	QPDHHSLKTFPAVNR CCCCHHCCCCCCCCC	51.65	17644757
303	Phosphorylation	PAVNRRDSMTPVEPR CCCCCCCCCCCCCCC	23.81	28152593
305	Phosphorylation	VNRRDSMTPVEPRNM CCCCCCCCCCCCCHH	28.30	21440633
317	Phosphorylation	RNMVKHKTSFSDFHK CHHHCCCCCHHHHHH	34.42	21551504
318	Phosphorylation	NMVKHKTSFSDFHKT HHHCCCCCHHHHHHH	27.79	17287358
320	Phosphorylation	VKHKTSFSDFHKTIF HCCCCCHHHHHHHHH	38.85	19779198
336	Phosphorylation	SNTYSESSDTISSME CCCCCCCCCCEEEEE	34.42	21551504
345	Phosphorylation	TISSMEITLKNKASS CEEEEEEEECCCCCC	20.55	21551504
351	Phosphorylation	ITLKNKASSSSLSLN EEECCCCCCCCCHHH	32.15	19823750
352	Phosphorylation	TLKNKASSSSLSLNV EECCCCCCCCCHHHH	28.89	19684113
353	Phosphorylation	LKNKASSSSLSLNVL ECCCCCCCCCHHHHH	32.57	20377248
354	Phosphorylation	KNKASSSSLSLNVLK CCCCCCCCCHHHHHH	24.75	19823750
356	Phosphorylation	KASSSSLSLNVLKKR CCCCCCCHHHHHHHC	21.61	20377248
365	Phosphorylation	NVLKKRNSQSSLKHK HHHHHCCCCHHHCCC	35.16	19795423
367	Phosphorylation	LKKRNSQSSLKHKSS HHHCCCCHHHCCCCC	37.14	19823750
368	Phosphorylation	KKRNSQSSLKHKSSH HHCCCCHHHCCCCCH	33.52	19795423
373	Phosphorylation	QSSLKHKSSHASLQK CHHHCCCCCHHHHHH	27.37	19823750
374	Phosphorylation	SSLKHKSSHASLQKF HHHCCCCCHHHHHHH	28.07	19795423
377	Phosphorylation	KHKSSHASLQKFKRN CCCCCHHHHHHHHHC	26.38	23749301
387	Ubiquitination	KFKRNKGKSSMIAPS HHHHCCCCCCCCCCC	40.75	17644757
395	Phosphorylation	SSMIAPSTATNSSND CCCCCCCCCCCCCCC	36.12	27017623
397	Phosphorylation	MIAPSTATNSSNDDS CCCCCCCCCCCCCCC	35.40	21440633
399	Phosphorylation	APSTATNSSNDDSCS CCCCCCCCCCCCCCC	26.60	28889911
404	Phosphorylation	TNSSNDDSCSYSSKN CCCCCCCCCCCCCCC	14.07	21440633
406	Phosphorylation	SSNDDSCSYSSKNST CCCCCCCCCCCCCCC	31.81	27017623
407	Phosphorylation	SNDDSCSYSSKNSTL CCCCCCCCCCCCCCC	22.69	23749301
408	Phosphorylation	NDDSCSYSSKNSTLS CCCCCCCCCCCCCCC	20.67	21440633
409	Phosphorylation	DDSCSYSSKNSTLSH CCCCCCCCCCCCCCE	27.85	23749301
410	Ubiquitination	DSCSYSSKNSTLSHR CCCCCCCCCCCCCEE	49.31	17644757
412	Phosphorylation	CSYSSKNSTLSHRIS CCCCCCCCCCCEEEE	34.17	28889911
413	Phosphorylation	SYSSKNSTLSHRISL CCCCCCCCCCEEEEC	41.49	23749301
415	Phosphorylation	SSKNSTLSHRISLPV CCCCCCCCEEEECCC	15.71	21440633
419	Phosphorylation	STLSHRISLPVPDQV CCCCEEEECCCCCCC	26.95	21440633
427	Phosphorylation	LPVPDQVSRDKIQNK CCCCCCCCHHHHHHH	29.18	23749301
437	Phosphorylation	KIQNKLRYSTSLLSL HHHHHHHHCCCHHHC	27.08	19823750
438	Phosphorylation	IQNKLRYSTSLLSLN HHHHHHHCCCHHHCC	12.70	21440633
439	Phosphorylation	QNKLRYSTSLLSLNS HHHHHHCCCHHHCCC	17.92	21440633
440	Phosphorylation	NKLRYSTSLLSLNSK HHHHHCCCHHHCCCC	22.75	20377248
443	Phosphorylation	RYSTSLLSLNSKSSL HHCCCHHHCCCCCCC	30.69	19684113
446	Phosphorylation	TSLLSLNSKSSLPMN CCHHHCCCCCCCCCC	38.93	21440633
448	Phosphorylation	LLSLNSKSSLPMNKN HHHCCCCCCCCCCCC	36.61	19823750
449	Phosphorylation	LSLNSKSSLPMNKND HHCCCCCCCCCCCCC	39.98	21440633
510	Ubiquitination	QIGEVICKKVSLGTI HHHHHHCEECCCCCC	45.12	17644757
511	Ubiquitination	IGEVICKKVSLGTID HHHHHCEECCCCCCC	32.42	17644757
525	Ubiquitination	DDITWDRKFLSLQEL CCCCCCCCCCCHHHH	48.46	17644757
541	Ubiquitination	KLKIMQQKFDGIPQL HHHHHHHHCCCHHHH	28.65	23749301
587	Ubiquitination	KNWKQILKIFWSCAG CCHHHHHHHHHHHHH	37.17	17644757
601	Ubiquitination	GIIHGLEKNLKFEHR HHHHHHHHHCCEECC	73.03	22817900
604	Ubiquitination	HGLEKNLKFEHRNLT HHHHHHCCEECCCEE	59.09	22817900
629	Ubiquitination	NITIIDFKCSRLQTP CEEEEEEECCCCCCC	27.79	17644757
735	Ubiquitination	EMNLAVHKRGGQLFK HHHHHHHHHHHHHHH	46.25	17644757

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ALK1_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ALK1_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ALK1_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KSS1_YEAST	KSS1	genetic	19269370
BPT1_YEAST	BPT1	physical	16093310
PDR18_YEAST	PDR18	physical	16093310
ALK2_YEAST	ALK2	genetic	23973165
QCR9_YEAST	QCR9	genetic	27708008
YAJ8_YEAST	YAR028W	genetic	27708008
RL4A_YEAST	RPL4A	genetic	27708008
YPQ3_YEAST	RTC2	genetic	27708008
YBP1_YEAST	YBP1	genetic	27708008
NHP10_YEAST	NHP10	genetic	27708008
GPR1_YEAST	GPR1	genetic	27708008
OMS1_YEAST	OMS1	genetic	27708008
ERJ5_YEAST	ERJ5	genetic	27708008
SAP4_YEAST	SAP4	genetic	27708008
YGY0_YEAST	YGL230C	genetic	27708008
EMC4_YEAST	EMC4	genetic	27708008
YIA6_YEAST	YIA6	genetic	27708008
FIS1_YEAST	FIS1	genetic	27708008
PIR5_YEAST	YJL160C	genetic	27708008
YJ24_YEAST	KCH1	genetic	27708008
CSN12_YEAST	YJR084W	genetic	27708008
ENV10_YEAST	ENV10	genetic	27708008
COA4_YEAST	COA4	genetic	27708008
YL456_YEAST	YLR456W	genetic	27708008
PT127_YEAST	PET127	genetic	27708008

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"A multidimensional chromatography technology for in-depthphosphoproteome analysis.";
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
Mol. Cell. Proteomics 7:1389-1396(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351 AND THR-439, ANDMASS SPECTROMETRY.
PubMed: 18407956

"Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry.";
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-172 AND SER-318, ANDMASS SPECTROMETRY.
PubMed: 17287358