| UniProt ID | ENT3_YEAST | |
|---|---|---|
| UniProt AC | P47160 | |
| Protein Name | Epsin-3 | |
| Gene Name | ENT3 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 408 | |
| Subcellular Localization |
Cytoplasm . Golgi apparatus, trans-Golgi network membrane Peripheral membrane protein . Cytoplasmic vesicle, clathrin-coated vesicle membrane Peripheral membrane protein . Associates with the trans Golgi network (TGN) and endosomal clathrin coats |
|
| Protein Description | Involved in the recruitment of clathrin to the Golgi network and endosomes to form clathrin coated vesicles. Plays a role in the trafficking of clathrin between the Golgi network and endosomes. Binds to membranes enriched in phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2) and, in association with VPS27, is involved in protein sorting at the multivesicular body (MVB).. | |
| Protein Sequence | MSLEDTLANMSLYDAKKYFRKAQNVVFNYTEMEGKVREATNNEPWGASSTLMDQISQGTYNFREREEILSMIFRRFTEKAGSEWRQIYKALQLLDYLIKHGSERFIDDTRNSINLIRILETFHYIDSQGRDQGINVRTRVKALIELLSDDNKIRAERKKARETAKKYKGVAGGSASADGSLNSKAGFTSTKVHGISVSADFDSDNEDNEDGSFSQNGYNDNASRATSTPGQGKQEPEDFVDFFSSESSKPSKELIQEDEKKADEEEDDDDEFSEFQSAVPVTNPANSFNLLNTSPIEGMPATTSSMPFYNSSTTDQGKITPAIAEPKKVDPFSSLFSTAKASAEAPSAPKASQAKAAASNPVSNSTTALSTDQDDDDEFGEMHGGAVQQEQNTNNNHTSSKEIDLLSF | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 2 | Acetylation | ------MSLEDTLAN ------CCHHHHHHH | 40.23 | 22814378 | |
| 2 | Phosphorylation | ------MSLEDTLAN ------CCHHHHHHH | 40.23 | 28152593 | |
| 6 | Phosphorylation | --MSLEDTLANMSLY --CCHHHHHHHCCHH | 20.56 | 21440633 | |
| 11 | Phosphorylation | EDTLANMSLYDAKKY HHHHHHCCHHHHHHH | 24.47 | 23607784 | |
| 13 | Phosphorylation | TLANMSLYDAKKYFR HHHHCCHHHHHHHHH | 13.42 | 28132839 | |
| 18 | Phosphorylation | SLYDAKKYFRKAQNV CHHHHHHHHHHHHCE | 14.12 | 19795423 | |
| 21 | Ubiquitination | DAKKYFRKAQNVVFN HHHHHHHHHHCEEEE | 44.41 | 24961812 | |
| 29 | Phosphorylation | AQNVVFNYTEMEGKV HHCEEEEEEEECCCE | 7.81 | 19795423 | |
| 30 | Phosphorylation | QNVVFNYTEMEGKVR HCEEEEEEEECCCEE | 29.93 | 19795423 | |
| 99 | Acetylation | QLLDYLIKHGSERFI HHHHHHHHHCCHHHC | 39.04 | 24489116 | |
| 112 | Phosphorylation | FIDDTRNSINLIRIL HCCCCCCHHHHHHHH | 15.01 | 27017623 | |
| 152 | Acetylation | ELLSDDNKIRAERKK HHHCCCHHHHHHHHH | 40.33 | 24489116 | |
| 152 | Ubiquitination | ELLSDDNKIRAERKK HHHCCCHHHHHHHHH | 40.33 | 23749301 | |
| 174 | Phosphorylation | YKGVAGGSASADGSL HCCCCCCCCCCCCCC | 20.38 | 22369663 | |
| 176 | Phosphorylation | GVAGGSASADGSLNS CCCCCCCCCCCCCCC | 28.70 | 22369663 | |
| 180 | Phosphorylation | GSASADGSLNSKAGF CCCCCCCCCCCCCCC | 25.56 | 22369663 | |
| 183 | Phosphorylation | SADGSLNSKAGFTST CCCCCCCCCCCCCCC | 29.22 | 24961812 | |
| 184 | Ubiquitination | ADGSLNSKAGFTSTK CCCCCCCCCCCCCCE | 52.00 | 23749301 | |
| 188 | Phosphorylation | LNSKAGFTSTKVHGI CCCCCCCCCCEEEEE | 34.27 | 27017623 | |
| 190 | Phosphorylation | SKAGFTSTKVHGISV CCCCCCCCEEEEEEE | 33.75 | 27017623 | |
| 196 | Phosphorylation | STKVHGISVSADFDS CCEEEEEEEEECCCC | 18.01 | 22369663 | |
| 198 | Phosphorylation | KVHGISVSADFDSDN EEEEEEEEECCCCCC | 18.48 | 22369663 | |
| 203 | Phosphorylation | SVSADFDSDNEDNED EEEECCCCCCCCCCC | 42.18 | 22369663 | |
| 212 | Phosphorylation | NEDNEDGSFSQNGYN CCCCCCCCCCCCCCC | 33.76 | 22369663 | |
| 214 | Phosphorylation | DNEDGSFSQNGYNDN CCCCCCCCCCCCCCC | 25.16 | 22369663 | |
| 218 | Phosphorylation | GSFSQNGYNDNASRA CCCCCCCCCCCCCCC | 27.22 | 22369663 | |
| 223 | Phosphorylation | NGYNDNASRATSTPG CCCCCCCCCCCCCCC | 28.80 | 22369663 | |
| 226 | Phosphorylation | NDNASRATSTPGQGK CCCCCCCCCCCCCCC | 31.83 | 22369663 | |
| 227 | Phosphorylation | DNASRATSTPGQGKQ CCCCCCCCCCCCCCC | 31.45 | 24909858 | |
| 228 | Phosphorylation | NASRATSTPGQGKQE CCCCCCCCCCCCCCC | 27.38 | 24909858 | |
| 244 | Phosphorylation | EDFVDFFSSESSKPS HHHHHHHCCCCCCCC | 32.94 | 22369663 | |
| 245 | Phosphorylation | DFVDFFSSESSKPSK HHHHHHCCCCCCCCH | 36.02 | 22369663 | |
| 247 | Phosphorylation | VDFFSSESSKPSKEL HHHHCCCCCCCCHHH | 45.68 | 22369663 | |
| 248 | Phosphorylation | DFFSSESSKPSKELI HHHCCCCCCCCHHHH | 44.53 | 22369663 | |
| 251 | Phosphorylation | SSESSKPSKELIQED CCCCCCCCHHHHHHH | 42.36 | 22369663 | |
| 294 | Phosphorylation | SFNLLNTSPIEGMPA CCCCCCCCCCCCCCC | 23.86 | 28889911 | |
| 320 | Phosphorylation | TTDQGKITPAIAEPK CCCCCCCCCCCCCCC | 15.34 | 22369663 | |
| 327 | Acetylation | TPAIAEPKKVDPFSS CCCCCCCCCCCCCHH | 57.86 | 24489116 | |
| 342 | Phosphorylation | LFSTAKASAEAPSAP HHHHHHHHCCCCCCC | 26.40 | 22369663 | |
| 347 | Phosphorylation | KASAEAPSAPKASQA HHHCCCCCCCHHHHH | 65.34 | 22369663 | |
| 352 | Phosphorylation | APSAPKASQAKAAAS CCCCCHHHHHHHHHC | 36.48 | 23749301 | |
| 363 | Phosphorylation | AAASNPVSNSTTALS HHHCCCCCCCCCCCC | 26.73 | 21440633 | |
| 365 | Phosphorylation | ASNPVSNSTTALSTD HCCCCCCCCCCCCCC | 21.45 | 23749301 | |
| 366 | Phosphorylation | SNPVSNSTTALSTDQ CCCCCCCCCCCCCCC | 21.97 | 24961812 | |
| 367 | Phosphorylation | NPVSNSTTALSTDQD CCCCCCCCCCCCCCC | 25.97 | 20377248 | |
| 370 | Phosphorylation | SNSTTALSTDQDDDD CCCCCCCCCCCCCCC | 27.72 | 20377248 | |
| 371 | Phosphorylation | NSTTALSTDQDDDDE CCCCCCCCCCCCCCC | 38.23 | 20377248 | |
| 393 | Phosphorylation | AVQQEQNTNNNHTSS HHEEECCCCCCCCCC | 38.73 | 21551504 | |
| 401 | Ubiquitination | NNNHTSSKEIDLLSF CCCCCCCCCEEECCC | 59.46 | 24961812 | |
| 407 | Phosphorylation | SKEIDLLSF------ CCCEEECCC------ | 35.89 | 22890988 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of ENT3_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of ENT3_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of ENT3_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203; SER-294; THR-320AND SER-365, AND MASS SPECTROMETRY. | |
| "Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae."; Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.; J. Proteome Res. 6:1190-1197(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, AND MASSSPECTROMETRY. | |
| "Quantitative phosphoproteomics applied to the yeast pheromonesignaling pathway."; Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J.,Mann M., Jensen O.N.; Mol. Cell. Proteomics 4:310-327(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407, AND MASSSPECTROMETRY. | |
