| UniProt ID | CLK1_HUMAN | |
|---|---|---|
| UniProt AC | P49759 | |
| Protein Name | Dual specificity protein kinase CLK1 | |
| Gene Name | CLK1 | |
| Organism | Homo sapiens (Human). | |
| Sequence Length | 484 | |
| Subcellular Localization | Nucleus. | |
| Protein Description | Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.. | |
| Protein Sequence | MRHSKRTYCPDWDDKDWDYGKWRSSSSHKRRKRSHSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHSRRYIDEYRNDYTQGCEPGHRQRDHESRYQNHSSKSSGRSGRSSYKSKHRIHHSTSHRRSHGKSHRRKRTRSVEDDEEGHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRRCKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRITLREALKHPFFDLLKKSI | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
|
|
||
* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 15 | Ubiquitination | YCPDWDDKDWDYGKW CCCCCCCCCCCCCCC | 59.24 | 29967540 | |
| 18 (in isoform 3) | Phosphorylation | - | 53.06 | - | |
| 21 | Sumoylation | DKDWDYGKWRSSSSH CCCCCCCCCCCCCHH | 32.95 | - | |
| 21 | Ubiquitination | DKDWDYGKWRSSSSH CCCCCCCCCCCCCHH | 32.95 | 29967540 | |
| 21 | Sumoylation | DKDWDYGKWRSSSSH CCCCCCCCCCCCCHH | 32.95 | - | |
| 24 | Phosphorylation | WDYGKWRSSSSHKRR CCCCCCCCCCHHHHH | 33.19 | - | |
| 34 | Phosphorylation | SHKRRKRSHSSAQEN HHHHHHCCCCHHHHH | 30.65 | 29514088 | |
| 36 | Phosphorylation | KRRKRSHSSAQENKR HHHHCCCCHHHHHHH | 28.72 | 28985074 | |
| 37 (in isoform 2) | Phosphorylation | - | 28.66 | - | |
| 37 | Phosphorylation | RRKRSHSSAQENKRC HHHCCCCHHHHHHHH | 28.66 | 17192257 | |
| 50 | Sumoylation | RCKYNHSKMCDSHYL HHCCCHHHHCCHHHH | 35.68 | - | |
| 50 | Ubiquitination | RCKYNHSKMCDSHYL HHCCCHHHHCCHHHH | 35.68 | 29967540 | |
| 50 | Sumoylation | RCKYNHSKMCDSHYL HHCCCHHHHCCHHHH | 35.68 | - | |
| 54 | Phosphorylation | NHSKMCDSHYLESRS CHHHHCCHHHHHCCC | 14.76 | 28555341 | |
| 56 | Phosphorylation | SKMCDSHYLESRSIN HHHCCHHHHHCCCCC | 19.00 | - | |
| 57 | Ubiquitination | KMCDSHYLESRSINE HHCCHHHHHCCCCCH | 3.94 | 29967540 | |
| 59 | Phosphorylation | CDSHYLESRSINEKD CCHHHHHCCCCCHHH | 28.82 | 24247654 | |
| 61 (in isoform 2) | Phosphorylation | - | 38.76 | - | |
| 61 | Phosphorylation | SHYLESRSINEKDYH HHHHHCCCCCHHHHH | 38.76 | 17192257 | |
| 63 | Ubiquitination | YLESRSINEKDYHSR HHHCCCCCHHHHHHH | 52.06 | 29967540 | |
| 65 | Ubiquitination | ESRSINEKDYHSRRY HCCCCCHHHHHHHHH | 60.13 | 29967540 | |
| 76 | Phosphorylation | SRRYIDEYRNDYTQG HHHHHHHHHCCCCCC | 15.80 | 29978859 | |
| 80 | Phosphorylation | IDEYRNDYTQGCEPG HHHHHCCCCCCCCCC | 12.43 | 29978859 | |
| 81 | Phosphorylation | DEYRNDYTQGCEPGH HHHHCCCCCCCCCCH | 22.73 | 25348954 | |
| 92 | Ubiquitination | EPGHRQRDHESRYQN CCCHHHCCCHHHHCC | 41.38 | 29967540 | |
| 98 (in isoform 3) | Phosphorylation | - | 39.80 | 27642862 | |
| 101 | Phosphorylation | ESRYQNHSSKSSGRS HHHHCCCCCCCCCCC | 47.19 | - | |
| 102 | Phosphorylation | SRYQNHSSKSSGRSG HHHCCCCCCCCCCCC | 29.07 | - | |
| 103 (in isoform 3) | Phosphorylation | - | 52.21 | 24719451 | |
| 104 | Phosphorylation | YQNHSSKSSGRSGRS HCCCCCCCCCCCCCC | 39.72 | - | |
| 107 | Ubiquitination | HSSKSSGRSGRSSYK CCCCCCCCCCCCCHH | 37.57 | 29967540 | |
| 108 | Phosphorylation | SSKSSGRSGRSSYKS CCCCCCCCCCCCHHH | 41.92 | 26657352 | |
| 122 | Phosphorylation | SKHRIHHSTSHRRSH HHCCCCCCHHHCCCC | 19.90 | 27732954 | |
| 123 | Phosphorylation | KHRIHHSTSHRRSHG HCCCCCCHHHCCCCC | 24.97 | 27732954 | |
| 124 | Phosphorylation | HRIHHSTSHRRSHGK CCCCCCHHHCCCCCC | 20.29 | 27732954 | |
| 138 | Phosphorylation | KSHRRKRTRSVEDDE CCCCCCCCCCCCCCC | 30.26 | 30266825 | |
| 140 | Phosphorylation | HRRKRTRSVEDDEEG CCCCCCCCCCCCCCC | 29.28 | 29255136 | |
| 153 | Phosphorylation | EGHLICQSGDVLSAR CCEEEECCCCEEEEE | 32.33 | 29632367 | |
| 158 | Phosphorylation | CQSGDVLSARYEIVD ECCCCEEEEEEEEEC | 15.56 | 20873877 | |
| 174 | Ubiquitination | LGEGAFGKVVECIDH CCCCCCCEEEEEHHC | 36.33 | 29967540 | |
| 180 | Phosphorylation | GKVVECIDHKAGGRH CEEEEEHHCCCCCCE | 49.22 | 33259812 | |
| 180 (in isoform 3) | Phosphorylation | - | 49.22 | 24719451 | |
| 182 (in isoform 3) | Phosphorylation | - | 45.00 | 24719451 | |
| 182 | Ubiquitination | VVECIDHKAGGRHVA EEEEHHCCCCCCEEE | 45.00 | 29967540 | |
| 191 | Sumoylation | GGRHVAVKIVKNVDR CCCEEEEEECCCHHH | 31.85 | - | |
| 191 | Sumoylation | GGRHVAVKIVKNVDR CCCEEEEEECCCHHH | 31.85 | - | |
| 191 | Ubiquitination | GGRHVAVKIVKNVDR CCCEEEEEECCCHHH | 31.85 | 29967540 | |
| 216 (in isoform 3) | Ubiquitination | - | 48.67 | - | |
| 216 | Ubiquitination | VLEHLNTTDPNSTFR HHHHHCCCCCCCCCH | 48.67 | 29967540 | |
| 220 | Phosphorylation | LNTTDPNSTFRCVQM HCCCCCCCCCHHHHH | 34.02 | 20071362 | |
| 221 | Phosphorylation | NTTDPNSTFRCVQML CCCCCCCCCHHHHHH | 23.03 | 20071362 | |
| 224 | Ubiquitination | DPNSTFRCVQMLEWF CCCCCCHHHHHHHHH | 1.86 | 29967540 | |
| 233 | Ubiquitination | QMLEWFEHHGHICIV HHHHHHHHCCCEEEE | 23.82 | 29967540 | |
| 285 | Phosphorylation | FLHSNKLTHTDLKPE CHHCCCCCCCCCCHH | 25.08 | 24719451 | |
| 290 | Ubiquitination | KLTHTDLKPENILFV CCCCCCCCHHHEEEE | 53.77 | 29967540 | |
| 299 | Phosphorylation | ENILFVQSDYTEAYN HHEEEEECCCCHHHC | 27.55 | 24719451 | |
| 302 | O-linked_Glycosylation | LFVQSDYTEAYNPKI EEEECCCCHHHCCCC | 21.09 | 30379171 | |
| 305 | Phosphorylation | QSDYTEAYNPKIKRD ECCCCHHHCCCCCCC | 26.26 | 24719451 | |
| 308 | Ubiquitination | YTEAYNPKIKRDERT CCHHHCCCCCCCCCC | 58.16 | 29967540 | |
| 322 | Sumoylation | TLINPDIKVVDFGSA CCCCCCCEEEEECCC | 43.52 | - | |
| 327 (in isoform 3) | Phosphorylation | - | 16.35 | 24719451 | |
| 328 | Phosphorylation | IKVVDFGSATYDDEH CEEEEECCCCCCCCC | 19.89 | 29255136 | |
| 330 | Phosphorylation | VVDFGSATYDDEHHS EEEECCCCCCCCCCC | 29.22 | 29255136 | |
| 331 | Phosphorylation | VDFGSATYDDEHHST EEECCCCCCCCCCCC | 22.55 | 29255136 | |
| 332 | Ubiquitination | DFGSATYDDEHHSTL EECCCCCCCCCCCCE | 50.93 | 29967540 | |
| 337 | Phosphorylation | TYDDEHHSTLVSTRH CCCCCCCCCEEECCC | 25.91 | 30266825 | |
| 338 | Phosphorylation | YDDEHHSTLVSTRHY CCCCCCCCEEECCCC | 27.16 | 30266825 | |
| 341 | Phosphorylation | EHHSTLVSTRHYRAP CCCCCEEECCCCCCC | 23.88 | 29255136 | |
| 341 (in isoform 3) | Phosphorylation | - | 23.88 | 24719451 | |
| 342 | Phosphorylation | HHSTLVSTRHYRAPE CCCCEEECCCCCCCH | 17.62 | 29255136 | |
| 345 | Phosphorylation | TLVSTRHYRAPEVIL CEEECCCCCCCHHHH | 12.74 | 30576142 | |
| 347 (in isoform 3) | Phosphorylation | - | 9.46 | 24719451 | |
| 350 | Ubiquitination | RHYRAPEVILALGWS CCCCCCHHHHHCCCC | 4.18 | 29967540 | |
| 372 (in isoform 3) | Phosphorylation | - | 9.30 | 24719451 | |
| 400 | Ubiquitination | RILGPLPKHMIQKTR HHHCCCCHHHHHHHC | 54.19 | 29967540 | |
| 442 | Ubiquitination | PLKEFMLSQDVEHER CHHHHCCCCCCCHHH | 16.73 | 29967540 | |
| 464 | Ubiquitination | MLEYDPAKRITLREA HHCCCHHHCCCHHHH | 50.12 | 29967540 | |
| 481 | Ubiquitination | HPFFDLLKKSI---- CHHHHHHHHCC---- | 52.34 | 29967540 | |
| 506 | Ubiquitination | ----------------------------- ----------------------------- | 29967540 | ||
| 506 (in isoform 3) | Ubiquitination | - | - | ||
| 523 | Ubiquitination | ---------------------------------------------- ---------------------------------------------- | 29967540 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of CLK1_HUMAN !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of CLK1_HUMAN !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of CLK1_HUMAN !! | ||||||
| Kegg Disease | ||||||
|---|---|---|---|---|---|---|
| There are no disease associations of PTM sites. | ||||||
| OMIM Disease | ||||||
| There are no disease associations of PTM sites. | ||||||
| Kegg Drug | ||||||
| There are no disease associations of PTM sites. | ||||||
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
loading...
| Phosphorylation | |
| Reference | PubMed |
| "Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61; THR-138; SER-140;THR-330; THR-338 AND SER-341, AND MASS SPECTROMETRY. | |
| "A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-138 AND SER-140, ANDMASS SPECTROMETRY. | |
| "Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37; SER-61; THR-138;SER-140; SER-341 AND THR-342, AND MASS SPECTROMETRY. | |
| "Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-138 AND SER-140, ANDMASS SPECTROMETRY. | |
