dbPTM

PRPK_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PRPK_HUMAN
UniProt AC	Q96S44
Protein Name	EKC/KEOPS complex subunit TP53RK {ECO:0000305}
Gene Name	TP53RK {ECO:0000312\|HGNC:HGNC:16197}
Organism	Homo sapiens (Human).
Sequence Length	253
Subcellular Localization	Cytoplasm . Nucleus .
Protein Description	Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. [PubMed: 22912744]
Protein Sequence	MAAARATTPADGEEPAPEAEALAAARERSSRFLSGLELVKQGAEARVFRGRFQGRAAVIKHRFPKGYRHPALEARLGRRRTVQEARALLRCRRAGISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQSTMETEKTPQGLSNLAKTIGQVLARMHDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLSTHPNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MAAARATTPADGEE -CCCCCCCCCCCCCC	27.97	29255136
8	Phosphorylation	MAAARATTPADGEEP CCCCCCCCCCCCCCC	18.59	29255136
34	Phosphorylation	ERSSRFLSGLELVKQ HHHHHHHHHHHHHHC	38.32	20068231
40	Ubiquitination	LSGLELVKQGAEARV HHHHHHHHCCCCEEE	56.49	21906983
121	Phosphorylation	EEIEGSVTVRDYIQS EEECCCEEHHHHHHH	16.12	24719451
129	Phosphorylation	VRDYIQSTMETEKTP HHHHHHHHCCCCCCC	11.58	19060867
132	Phosphorylation	YIQSTMETEKTPQGL HHHHHCCCCCCCCHH	31.77	19060867
134	Ubiquitination	QSTMETEKTPQGLSN HHHCCCCCCCCHHHH	73.29	21906983
135	Phosphorylation	STMETEKTPQGLSNL HHCCCCCCCCHHHHH	18.02	25159151
140	Phosphorylation	EKTPQGLSNLAKTIG CCCCCHHHHHHHHHH	36.30	22817900
144	Ubiquitination	QGLSNLAKTIGQVLA CHHHHHHHHHHHHHH	43.79	21890473
201	Phosphorylation	EDKGVDLYVLEKAFL CCCCCCEEEEEHHHH	9.87	22817900
214	Phosphorylation	FLSTHPNTETVFEAF HHHCCCCHHHHHHHH	37.17	28985074
226	Phosphorylation	EAFLKSYSTSSKKAR HHHHHHCCCCCCCHH	28.98	28985074
238	Ubiquitination	KARPVLKKLDEVRLR CHHHHHHHHHHHHHC	57.94	24816145
250	Phosphorylation	RLRGRKRSMVG---- HHCCCCCCCCC----	22.36	17712528

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
250	S	Phosphorylation	Kinase	AKT1	P31749	PSP
250	S	Phosphorylation	Kinase	PBK	Q96KB5	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of PRPK_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PRPK_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TPRKB_HUMAN	TPRKB	physical	12659830
P53_HUMAN	TP53	physical	12659830
TPRKB_HUMAN	TPRKB	physical	22912744
OSGEP_HUMAN	OSGEP	physical	22912744
LAGE3_HUMAN	LAGE3	physical	22912744
GON7_HUMAN	C14orf142	physical	22912744
TPRKB_HUMAN	TPRKB	physical	22939629
AASD1_HUMAN	AARSD1	physical	22863883
KBP_HUMAN	KIAA1279	physical	22863883
TRMB_HUMAN	METTL1	physical	22863883
NIF3L_HUMAN	NIF3L1	physical	22863883
NSUN2_HUMAN	NSUN2	physical	22863883
PANK4_HUMAN	PANK4	physical	22863883
KAPCB_HUMAN	PRKACB	physical	22863883
RAGP1_HUMAN	RANGAP1	physical	22863883
SAE1_HUMAN	SAE1	physical	22863883
SH3G1_HUMAN	SH3GL1	physical	22863883
THG1_HUMAN	THG1L	physical	22863883
TM1L1_HUMAN	TOM1L1	physical	22863883
TPD54_HUMAN	TPD52L2	physical	22863883
UFM1_HUMAN	UFM1	physical	22863883
SPSY_HUMAN	SMS	physical	23455922
LAGE3_HUMAN	LAGE3	physical	23455922
NUP43_HUMAN	NUP43	physical	23455922
INADL_HUMAN	INADL	physical	23455922
GON7_HUMAN	C14orf142	physical	23455922
OSGEP_HUMAN	OSGEP	physical	23455922
TPRKB_HUMAN	TPRKB	physical	23455922
TPRKB_HUMAN	TPRKB	physical	26186194
GON7_HUMAN	C14orf142	physical	26186194
OSGEP_HUMAN	OSGEP	physical	26186194
NUP43_HUMAN	NUP43	physical	26186194
ARP19_HUMAN	ARPP19	physical	26344197
PRPS1_HUMAN	PRPS1	physical	26344197
WDR4_HUMAN	WDR4	physical	26344197
GON7_HUMAN	C14orf142	physical	28514442
OSGEP_HUMAN	OSGEP	physical	28514442
TPRKB_HUMAN	TPRKB	physical	28514442
NUP43_HUMAN	NUP43	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PRPK_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-7; THR-8; SER-34;THR-129; THR-132; THR-135; SER-140 AND TYR-201, AND MASS SPECTROMETRY.	19369195 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-135, AND MASSSPECTROMETRY.	18691976 [Abstract]