dbPTM

NF1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NF1_HUMAN
UniProt AC	P21359
Protein Name	Neurofibromin
Gene Name	NF1
Organism	Homo sapiens (Human).
Sequence Length	2839
Subcellular Localization	Nucleus . Nucleus, nucleolus .
Protein Description	Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity..
Protein Sequence	MAAHRPVEWVQAVVSRFDEQLPIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHFLLFYGVGCDIPSSGNTSQMSMDHEELLRTPGASLRKGKGNSSMDSAAGCSGTPPICRQAQTKLEVALYMFLWNPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNLLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSNSGLATYSPPMGPVSERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDESAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEVDPTRLEPSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDAVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLVFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGTALLEQNLHTLDSLRIFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKETQPWSSPKGSEGYLAATYPTVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPALQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAHRPVEW ------CCCCCCHHH	24.23	-
15	Phosphorylation	EWVQAVVSRFDEQLP HHHHHHHHHCHHCCC	22.19	30631047
24	Ubiquitination	FDEQLPIKTGQQNTH CHHCCCCCCCCCCCC	44.67	29967540
40	Ubiquitination	KVSTEHNKECLINIS EECCCCCCCEEEEHH	52.27	29967540
52	Phosphorylation	NISKYKFSLVISGLT EHHHHHHEEHHHCHH	19.99	20068231
56	Phosphorylation	YKFSLVISGLTTILK HHHEEHHHCHHHHHH	21.92	20068231
59	Phosphorylation	SLVISGLTTILKNVN EEHHHCHHHHHHHHC	18.47	20068231
60	Phosphorylation	LVISGLTTILKNVNN EHHHCHHHHHHHHCC	29.38	20068231
107	Phosphorylation	DTMRLDETMLVKQLL CCCCCCHHHHHHHHH	18.57	23403867
146	Phosphorylation	SGVLFSLSCNNFNAV CCEEEEEECCCHHHH	17.38	22468782
155	Phosphorylation	NNFNAVFSRISTRLQ CCHHHHHHHHHHHHH	23.31	22468782
261	Ubiquitination	DGFAESTKRKAAVWP HHHHHHHHHHHCHHH	62.27	29967540
297	Ubiquitination	DENNMNKKLFLDSLR CCCCCCHHHHHHHHH	39.19	29967540
302	Phosphorylation	NKKLFLDSLRKALAG CHHHHHHHHHHHHCC	32.02	26091039
361	Phosphorylation	KNLLFNPSKPFSRGS HHHCCCCCCCCCCCC	54.81	29396449
362	Ubiquitination	NLLFNPSKPFSRGSQ HHCCCCCCCCCCCCC	51.87	23000965
449	Ubiquitination	MFGETLHKAVQGCGA HHHHHHHHHHCCCCC	54.06	-
465	Phosphorylation	PAIRMAPSLTFKEKV HHHHCCCCCCHHHHH	30.42	20860994
467	Phosphorylation	IRMAPSLTFKEKVTS HHCCCCCCHHHHHHC	36.68	20860994
469	Methylation	MAPSLTFKEKVTSLK CCCCCCHHHHHHCCC	51.93	115973915
469	Ubiquitination	MAPSLTFKEKVTSLK CCCCCCHHHHHHCCC	51.93	33845483
471	Methylation	PSLTFKEKVTSLKFK CCCCHHHHHHCCCCC	51.94	15612473
471	Ubiquitination	PSLTFKEKVTSLKFK CCCCHHHHHHCCCCC	51.94	-
476	Methylation	KEKVTSLKFKEKPTD HHHHHCCCCCCCCCC	54.91	15612485
476	Ubiquitination	KEKVTSLKFKEKPTD HHHHHCCCCCCCCCC	54.91	33845483
478	Ubiquitination	KVTSLKFKEKPTDLE HHHCCCCCCCCCCCC	64.34	29967540
480	Ubiquitination	TSLKFKEKPTDLETR HCCCCCCCCCCCCHH	54.53	33845483
491	Phosphorylation	LETRSYKYLLLSMVK CCHHHHHHHHHHHHH	8.37	-
505	Ubiquitination	KLIHADPKLLLCNPR HHHHCCCCEEECCCC	52.92	29967540
575 (in isoform 5)	Phosphorylation	-	25.85	25072903
577 (in isoform 5)	Phosphorylation	-	2.78	25072903
579 (in isoform 5)	Phosphorylation	-	3.03	25072903
583 (in isoform 5)	Phosphorylation	-	41.06	25072903
584 (in isoform 5)	Phosphorylation	-	53.22	25072903
593 (in isoform 5)	Phosphorylation	-	26.54	-
620	Phosphorylation	KNKQADRSSCHFLLF CCCCCCHHCCEEEEE	37.55	27251275
621	Phosphorylation	NKQADRSSCHFLLFY CCCCCHHCCEEEEEE	16.71	27251275
628	Phosphorylation	SCHFLLFYGVGCDIP CCEEEEEEECCCCCC	15.75	27642862
653	Phosphorylation	DHEELLRTPGASLRK CHHHHHCCCCCCCCC	26.50	23186163
657	Phosphorylation	LLRTPGASLRKGKGN HHCCCCCCCCCCCCC	34.32	27422710
660	Ubiquitination	TPGASLRKGKGNSSM CCCCCCCCCCCCCCC	70.98	22817900
662	Ubiquitination	GASLRKGKGNSSMDS CCCCCCCCCCCCCHH	58.93	21963094
665	Phosphorylation	LRKGKGNSSMDSAAG CCCCCCCCCCHHCCC	35.39	26471730
666	Phosphorylation	RKGKGNSSMDSAAGC CCCCCCCCCHHCCCC	30.52	26471730
669	Phosphorylation	KGNSSMDSAAGCSGT CCCCCCHHCCCCCCC	16.04	26471730
669	Ubiquitination	KGNSSMDSAAGCSGT CCCCCCHHCCCCCCC	16.04	22817900
670	Ubiquitination	GNSSMDSAAGCSGTP CCCCCHHCCCCCCCC	12.01	22817900
671	Ubiquitination	NSSMDSAAGCSGTPP CCCCHHCCCCCCCCH	24.39	21963094
672	Ubiquitination	SSMDSAAGCSGTPPI CCCHHCCCCCCCCHH	13.07	21963094
674	Phosphorylation	MDSAAGCSGTPPICR CHHCCCCCCCCHHHH	44.79	-
676	Phosphorylation	SAAGCSGTPPICRQA HCCCCCCCCHHHHHH	15.03	-
783	Ubiquitination	AWEDTHAKWEQATKL HHHHHHHHHHHHHHH	43.59	29967540
796	Ubiquitination	KLILNYPKAKMEDGQ HHHHHCCHHHCCCCH	50.72	29967540
798	Ubiquitination	ILNYPKAKMEDGQAA HHHCCHHHCCCCHHH	49.28	-
807	Phosphorylation	EDGQAAESLHKTIVK CCCHHHHHHHHHHHH	30.92	29255136
810	Ubiquitination	QAAESLHKTIVKRRM HHHHHHHHHHHHHHH	45.28	33845483
811	O-linked_Glycosylation	AAESLHKTIVKRRMS HHHHHHHHHHHHHHC	21.91	31492838
856	Phosphorylation	GVCLQQRSNSGLATY CEEEECCCCCCCCCC	30.89	21945579
858	Phosphorylation	CLQQRSNSGLATYSP EEECCCCCCCCCCCC	36.11	21945579
862	Phosphorylation	RSNSGLATYSPPMGP CCCCCCCCCCCCCCC	29.58	21945579
863	Phosphorylation	SNSGLATYSPPMGPV CCCCCCCCCCCCCCC	17.36	21945579
864	Phosphorylation	NSGLATYSPPMGPVS CCCCCCCCCCCCCCC	20.41	19664994
871	Phosphorylation	SPPMGPVSERKGSMI CCCCCCCCCCCCCEE	34.51	21945579
874	Ubiquitination	MGPVSERKGSMISVM CCCCCCCCCCEEEEE	51.81	-
876	Phosphorylation	PVSERKGSMISVMSS CCCCCCCCEEEEECC	18.91	23401153
879	Phosphorylation	ERKGSMISVMSSEGN CCCCCEEEEECCCCC	11.79	29255136
882	Phosphorylation	GSMISVMSSEGNADT CCEEEEECCCCCCCC	24.07	29255136
883	Phosphorylation	SMISVMSSEGNADTP CEEEEECCCCCCCCH	31.28	29255136
889	Phosphorylation	SSEGNADTPVSKFMD CCCCCCCCHHHHHHH	24.15	25850435
892	Phosphorylation	GNADTPVSKFMDRLL CCCCCHHHHHHHHHH	22.75	23186163
915	Phosphorylation	KVGLQIRTNVKDLVG CCCEEEECCHHHHCC	46.09	-
926	Phosphorylation	DLVGLELSPALYPML HHCCCCCCHHHHHHH	9.96	-
930	Phosphorylation	LELSPALYPMLFNKL CCCCHHHHHHHHHHH	6.68	-
1036	Ubiquitination	LSFCQEMKFRNKMVE CHHHHHHHHHHHHHH	40.18	-
1052	Phosphorylation	LTDWVMGTSNQAADD HHHHHHCCCCCCCCC	13.35	28985074
1053	Phosphorylation	TDWVMGTSNQAADDD HHHHHCCCCCCCCCC	22.63	28985074
1140	Phosphorylation	GMSRRLASLRHCTVL HHHHHHHHHHHHHHH	30.44	25394399
1204	Methylation	AETVLADRFERLVEL HHHHHHHHHHHHHHH	29.71	18567601
1283	Acetylation	RGNSLASKIMTFCFK CCCHHHHHHHHHHHH	30.78	19821473
1375	Phosphorylation	LYQATCHSLLNKATV HHHHHHHHHHHHHCH	36.38	24719451
1440	Malonylation	PRIERGLKLMSKILQ CHHHHHHHHHHHHHH	44.99	32601280
1456	Phosphorylation	IANHVLFTKEEHMRP HHHCCCCCCHHHCCC	33.13	18452278
1496	Ubiquitination	SDAVNHSLSFISDGN HHHCCCCEEECCCCC	3.64	21890473
1496 (in isoform 2)	Ubiquitination	-	3.64	21890473
1506	Ubiquitination	ISDGNVLALHRLLWN CCCCCHHEEHHHHHC	9.31	21890473
1517	Ubiquitination	LLWNNQEKIGQYLSS HHHCCHHHHHHHHHC	42.23	22817900
1517 (in isoform 1)	Ubiquitination	-	42.23	21890473
1517 (in isoform 4)	Ubiquitination	-	42.23	21890473
1521	Phosphorylation	NQEKIGQYLSSNRDH CHHHHHHHHHCCCCC	11.88	25147952
1523	Phosphorylation	EKIGQYLSSNRDHKA HHHHHHHHCCCCCCC	22.19	24961811
1526	Ubiquitination	GQYLSSNRDHKAVGR HHHHHCCCCCCCCCC	49.73	21890473
1527	Ubiquitination	QYLSSNRDHKAVGRR HHHHCCCCCCCCCCC	52.46	21890473
1560	Acetylation	PVADTHWSSLNLTSS CCCCCCHHHCCCCHH	19.83	19608861
1567	Phosphorylation	SSLNLTSSKFEEFMT HHCCCCHHHHHHHHH	35.81	-
1581	Acetylation	TRHQVHEKEEFKALK HHCCCCCHHHHHHHH	47.90	19608861
1589	Phosphorylation	EEFKALKTLSIFYQA HHHHHHHHHHHHHHC	26.96	28258704
1680	Ubiquitination	NVSAVYIYNCNSWVR CEEEEEEEECCHHHH	8.93	29967540
1701	Ubiquitination	ERLLTGLKGSKRLVF HHHHHCCCCCCEEEE	63.58	29967540
1724	Ubiquitination	EHIEHEQQKLPAATL HHHHHHHHHCCHHHH	46.39	29967540
1730	Phosphorylation	QQKLPAATLALEEDL HHHCCHHHHHHHHHH	18.05	29396449
1745	Ubiquitination	KVFHNALKLAHKDTK HHHHHHHHHHCCCCC	41.01	29967540
1751	Phosphorylation	LKLAHKDTKVSIKVG HHHHCCCCCEEEEEC	37.84	-
1834	Phosphorylation	IRTRWELSQPDSIPQ HHHCHHHCCCCCCCC	28.31	27461979
1838	Phosphorylation	WELSQPDSIPQHTKI HHHCCCCCCCCCCCC	42.74	27461979
2013	Phosphorylation	LLDVVLDSFIKTSAT HHHHHHHHHHHCCCC	25.39	24719451
2028	Ubiquitination	GGLGSIKAEVMADTA CCCCCHHHHHHHHHH	16.97	29967540
2040	Phosphorylation	DTAVALASGNVKLVS HHHHHHHCCCEEEEC	31.58	25599653
2049	Ubiquitination	NVKLVSSKVIGRMCK CEEEECHHHHHHHHH	30.30	29967540
2157	Phosphorylation	RLSLTEFSLPKFYLL HHHCHHCCCCHHHHH	37.21	24719451
2180	Phosphorylation	AAVIAFRSSYRDRSF EEEEEECCCCCCCCC	25.85	23312004
2181	Phosphorylation	AVIAFRSSYRDRSFS EEEEECCCCCCCCCC	21.67	23312004
2182	Phosphorylation	VIAFRSSYRDRSFSP EEEECCCCCCCCCCC	20.13	23312004
2186	Phosphorylation	RSSYRDRSFSPGSYE CCCCCCCCCCCCCHH	34.07	19691289
2188	Phosphorylation	SYRDRSFSPGSYERE CCCCCCCCCCCHHHC	29.60	25159151
2191	Phosphorylation	DRSFSPGSYERETFA CCCCCCCCHHHCCEE	27.49	19691289
2192	Phosphorylation	RSFSPGSYERETFAL CCCCCCCHHHCCEEE	25.56	19691289
2196	Phosphorylation	PGSYERETFALTSLE CCCHHHCCEEECCHH	22.04	24043423
2200	Phosphorylation	ERETFALTSLETVTE HHCCEEECCHHHHHH	28.03	24043423
2201	Phosphorylation	RETFALTSLETVTEA HCCEEECCHHHHHHH	25.67	24043423
2204	Phosphorylation	FALTSLETVTEALLE EEECCHHHHHHHHHH	37.71	24043423
2206	Phosphorylation	LTSLETVTEALLEIM ECCHHHHHHHHHHHH	23.98	24043423
2299	Phosphorylation	EATVIALTKLQPLLN HHHHHHHHHHHHHHC	22.23	22210691
2444	Phosphorylation	AYLAALLTVSEEVRS HHHHHHHHCCHHHHH	23.88	-
2446	Phosphorylation	LAALLTVSEEVRSRC HHHHHHCCHHHHHHC	23.86	-
2451	Phosphorylation	TVSEEVRSRCSLKHR HCCHHHHHHCCHHCC	42.70	-
2460	Phosphorylation	CSLKHRKSLLLTDIS CCHHCCCHHEEEECC	24.98	22617229
2464	Phosphorylation	HRKSLLLTDISMENV CCCHHEEEECCCCCC	31.12	27732954
2467	Phosphorylation	SLLLTDISMENVPMD HHEEEECCCCCCCCC	23.89	-
2468	Ubiquitination	LLLTDISMENVPMDT HEEEECCCCCCCCCC	4.42	29967540
2476	Phosphorylation	ENVPMDTYPIHHGDP CCCCCCCCCCCCCCC	8.91	22817900
2484	Phosphorylation	PIHHGDPSYRTLKET CCCCCCCHHCCCCCC	31.80	27642862
2485	Phosphorylation	IHHGDPSYRTLKETQ CCCCCCHHCCCCCCC	17.01	27642862
2487	Phosphorylation	HGDPSYRTLKETQPW CCCCHHCCCCCCCCC	33.67	27174698
2489	Methylation	DPSYRTLKETQPWSS CCHHCCCCCCCCCCC	59.09	115973907
2489	Ubiquitination	DPSYRTLKETQPWSS CCHHCCCCCCCCCCC	59.09	29967540
2491	Phosphorylation	SYRTLKETQPWSSPK HHCCCCCCCCCCCCC	38.04	28450419
2494	Phosphorylation	TLKETQPWSSPKGSE CCCCCCCCCCCCCCC	11.13	32142685
2495	Phosphorylation	LKETQPWSSPKGSEG CCCCCCCCCCCCCCC	43.37	30266825
2496	Phosphorylation	KETQPWSSPKGSEGY CCCCCCCCCCCCCCE	27.11	30266825
2500	Phosphorylation	PWSSPKGSEGYLAAT CCCCCCCCCCEEEEE	33.69	21945579
2503	Phosphorylation	SPKGSEGYLAATYPT CCCCCCCEEEEECCC	6.79	21945579
2507	Phosphorylation	SEGYLAATYPTVGQT CCCEEEEECCCCCCC	25.15	21945579
2508	Phosphorylation	EGYLAATYPTVGQTS CCEEEEECCCCCCCC	7.68	21945579
2510	Phosphorylation	YLAATYPTVGQTSPR EEEEECCCCCCCCCC	27.19	21945579
2514	Phosphorylation	TYPTVGQTSPRARKS ECCCCCCCCCCCHHH	34.47	22167270
2515	Phosphorylation	YPTVGQTSPRARKSM CCCCCCCCCCCHHHC	12.42	22167270
2521	Phosphorylation	TSPRARKSMSLDMGQ CCCCCHHHCCCCCCC	14.19	29255136
2522	Phosphorylation	SPRARKSMSLDMGQP CCCCHHHCCCCCCCC	5.31	32142685
2523	Phosphorylation	PRARKSMSLDMGQPS CCCHHHCCCCCCCCC	28.45	29255136
2530	Phosphorylation	SLDMGQPSQANTKKL CCCCCCCCCCCHHHH	33.75	23927012
2540	Phosphorylation	NTKKLLGTRKSFDHL CHHHHHCCHHHHHHH	34.59	29214152
2543	Phosphorylation	KLLGTRKSFDHLISD HHHCCHHHHHHHHCC	32.39	29255136
2544	Phosphorylation	LLGTRKSFDHLISDT HHCCHHHHHHHHCCC	8.44	32645325
2549	Phosphorylation	KSFDHLISDTKAPKR HHHHHHHCCCCCCCH	45.66	23927012
2551	Phosphorylation	FDHLISDTKAPKRQE HHHHHCCCCCCCHHH	23.47	23312004
2561	Phosphorylation	PKRQEMESGITTPPK CCHHHHHCCCCCCHH	33.90	28176443
2564	Phosphorylation	QEMESGITTPPKMRR HHHHCCCCCCHHHHH	36.45	30266825
2565	Phosphorylation	EMESGITTPPKMRRV HHHCCCCCCHHHHHH	35.36	23401153
2574	Ubiquitination	PKMRRVAETDYEMET HHHHHHHCCCHHHHH	39.08	29967540
2575	Phosphorylation	KMRRVAETDYEMETQ HHHHHHCCCHHHHHH	33.86	21945579
2577	Phosphorylation	RRVAETDYEMETQRI HHHHCCCHHHHHHHC	25.29	21945579
2580	Phosphorylation	AETDYEMETQRISSS HCCCHHHHHHHCCCC	29.83	32142685
2581	Phosphorylation	ETDYEMETQRISSSQ CCCHHHHHHHCCCCC	23.10	30576142
2585	Phosphorylation	EMETQRISSSQQHPH HHHHHHCCCCCCCCC	26.56	28555341
2586	Phosphorylation	METQRISSSQQHPHL HHHHHCCCCCCCCCC	29.78	25627689
2587	Phosphorylation	ETQRISSSQQHPHLR HHHHCCCCCCCCCCE	27.37	25159151
2595	Ubiquitination	QQHPHLRKVSVSESN CCCCCCEECCCCCCC	45.26	29967540
2597	Phosphorylation	HPHLRKVSVSESNVL CCCCEECCCCCCCCC	23.98	30266825
2599	Phosphorylation	HLRKVSVSESNVLLD CCEECCCCCCCCCCC	28.00	30266825
2601	Phosphorylation	RKVSVSESNVLLDEE EECCCCCCCCCCCHH	25.40	29255136
2611	Phosphorylation	LLDEEVLTDPKIQAL CCCHHHCCCHHHHHH	56.27	23927012
2781	Phosphorylation	ANLNLSNSMTSLATS EECCCCCCHHHHHHC	21.77	20068231
2799 (in isoform 6)	Phosphorylation	-	9.09	25849741
2802	Phosphorylation	DKENVELSPTTGHCN CHHCEEECCCCCCCC	13.49	29255136
2804	Phosphorylation	ENVELSPTTGHCNSG HCEEECCCCCCCCCC	41.87	29255136
2805	Phosphorylation	NVELSPTTGHCNSGR CEEECCCCCCCCCCC	28.45	21815630
2810	Phosphorylation	PTTGHCNSGRTRHGS CCCCCCCCCCCCCCC	34.74	25159151
2813	Phosphorylation	GHCNSGRTRHGSASQ CCCCCCCCCCCCHHH	30.46	29514088
2817	Phosphorylation	SGRTRHGSASQVQKQ CCCCCCCCHHHHHHH	20.72	28176443
2819	Phosphorylation	RTRHGSASQVQKQRS CCCCCCHHHHHHHHH	32.36	30576142
2826	Phosphorylation	SQVQKQRSAGSFKRN HHHHHHHHCCCCCHH	34.19	33259812
2829	Phosphorylation	QKQRSAGSFKRNSIK HHHHHCCCCCHHHCH	27.58	28102081
2834	Phosphorylation	AGSFKRNSIKKIV-- CCCCCHHHCHHCC--	39.56	26074081

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
2808	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	FAF2	Q96CS3	PMID:20160012
-	K	Ubiquitination	E3 ubiquitin ligase	FBXW7	Q969H0	PMID:24658274
-	K	Ubiquitination	E3 ubiquitin ligase	SAG	P10523	PMID:23136067

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NF1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NF1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RPB1_HUMAN	POLR2A	physical	15999204
SMCA4_HUMAN	SMARCA4	physical	15999204
TERA_HUMAN	VCP	physical	22105171
NSF1C_HUMAN	NSFL1C	physical	22105171
FAF2_HUMAN	FAF2	physical	20160012
NOSIP_HUMAN	NOSIP	physical	26344197
A4_HUMAN	APP	physical	16374483
1433F_HUMAN	YWHAH	physical	14741381
PML_HUMAN	PML	physical	22293200
PTEN_HUMAN	PTEN	genetic	28319113
SDC2_HUMAN	SDC2	physical	11356864
KI13B_HUMAN	KIF13B	physical	27173435
ZBT21_HUMAN	ZBTB21	physical	27173435
CING_HUMAN	CGN	physical	27173435
GGYF1_HUMAN	GIGYF1	physical	27173435
LRFN1_HUMAN	LRFN1	physical	27173435
SRGP2_HUMAN	SRGAP2	physical	27173435
SI1L1_HUMAN	SIPA1L1	physical	27173435
LIMA1_HUMAN	LIMA1	physical	27173435
RGPS2_HUMAN	RALGPS2	physical	27173435
MAGI1_HUMAN	MAGI1	physical	27173435
CBY1_HUMAN	CBY1	physical	27173435
TESK2_HUMAN	TESK2	physical	27173435
DCLK1_HUMAN	DCLK1	physical	27173435
GGYF2_HUMAN	GIGYF2	physical	27173435
HDAC4_HUMAN	HDAC4	physical	27173435
NADK_HUMAN	NADK	physical	27173435
SRS12_HUMAN	SRSF12	physical	27173435
NGAP_HUMAN	RASAL2	physical	27173435
SYDE1_HUMAN	SYDE1	physical	27173435
AGAP1_HUMAN	AGAP1	physical	27173435
PKHA7_HUMAN	PLEKHA7	physical	27173435
KIF1C_HUMAN	KIF1C	physical	27173435
F110B_HUMAN	FAM110B	physical	27173435
UBP21_HUMAN	USP21	physical	27173435
NAV1_HUMAN	NAV1	physical	27173435
STA13_HUMAN	STARD13	physical	27173435
RASN_HUMAN	NRAS	physical	7499408

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
162200	Neurofibromatosis 1 (NF1)
607785	Leukemia, juvenile myelomonocytic (JMML)
193520	Watson syndrome (WS)
162210	Familial spinal neurofibromatosis (FSNF)
601321	Neurofibromatosis-Noonan syndrome (NFNS)
114500	Colorectal cancer (CRC)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NF1_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-1581, AND MASS SPECTROMETRY.	19608861 [Abstract]
Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-864; SER-876 ANDSER-2515, AND MASS SPECTROMETRY.	19690332 [Abstract]
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2515; SER-2543 ANDSER-2597, AND MASS SPECTROMETRY.	19369195 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-864 AND SER-2523, ANDMASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-864; SER-2188; SER-2515;SER-2521 AND SER-2543, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2188; SER-2496 ANDSER-2543, AND MASS SPECTROMETRY.	18691976 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2515, AND MASSSPECTROMETRY.	18220336 [Abstract]
"Phosphoproteome analysis of the human mitotic spindle."; Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R.; Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2188, AND MASSSPECTROMETRY.	16565220 [Abstract]
"Global phosphoproteome of HT-29 human colon adenocarcinoma cells."; Kim J.-E., Tannenbaum S.R., White F.M.; J. Proteome Res. 4:1339-1346(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2829, AND MASSSPECTROMETRY.	16083285 [Abstract]
"Large-scale characterization of HeLa cell nuclear phosphoproteins."; Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-864 AND SER-2515, ANDMASS SPECTROMETRY.	15302935 [Abstract]
"Multiple reaction monitoring for robust quantitative proteomicanalysis of cellular signaling networks."; Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M.; Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-2577, AND MASSSPECTROMETRY.	17389395 [Abstract]
"Global survey of phosphotyrosine signaling identifies oncogenickinases in lung cancer."; Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J.,Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L.,Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J.,Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X.,Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.; Cell 131:1190-1203(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-2476, AND MASSSPECTROMETRY.	18083107 [Abstract]