dbPTM

CXA1_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CXA1_RAT
UniProt AC	P08050
Protein Name	Gap junction alpha-1 protein
Gene Name	Gja1
Organism	Rattus norvegicus (Rat).
Sequence Length	382
Subcellular Localization	Cell membrane Multi-pass membrane protein . Cell junction, gap junction . Endoplasmic reticulum . Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65.
Protein Description	Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. [PubMed: 15181016 Plays an essential role in gap junction communication in the ventricles (By similarity]
Protein Sequence	MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVEMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNAKKVAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---MGDWSALGKLLD ---CCCHHHHHHHHH	20.53	22673903
109	Acetylation	KEEKLNKKEEELKVA HHHHHCHHHHHHHHH	70.10	22902405
109	Ubiquitination	KEEKLNKKEEELKVA HHHHHCHHHHHHHHH	70.10	-
128	Ubiquitination	VNVEMHLKQIEIKKF CCCEEEEEEEEEEEC	34.25	-
133	Acetylation	HLKQIEIKKFKYGIE EEEEEEEEECCCCHH	39.09	22902405
134	Ubiquitination	LKQIEIKKFKYGIEE EEEEEEEECCCCHHH	53.39	-
136	Ubiquitination	QIEIKKFKYGIEEHG EEEEEECCCCHHHCC	51.99	-
137	Phosphorylation	IEIKKFKYGIEEHGK EEEEECCCCHHHCCC	26.86	-
144	Acetylation	YGIEEHGKVKMRGGL CCHHHCCCEEECCHH	40.36	22902405
244	Phosphorylation	KDRVKGRSDPYHATT HHHHCCCCCCCCCCC	52.16	23991683
247	Phosphorylation	VKGRSDPYHATTGPL HCCCCCCCCCCCCCC	14.74	14681032
250	Phosphorylation	RSDPYHATTGPLSPS CCCCCCCCCCCCCCC	20.97	27097102
251	Phosphorylation	SDPYHATTGPLSPSK CCCCCCCCCCCCCCC	37.27	27097102
255	Phosphorylation	HATTGPLSPSKDCGS CCCCCCCCCCCCCCC	30.20	23991683
257	Phosphorylation	TTGPLSPSKDCGSPK CCCCCCCCCCCCCCC	37.44	16000550
258	Ubiquitination	TGPLSPSKDCGSPKY CCCCCCCCCCCCCCE	61.49	-
262	Phosphorylation	SPSKDCGSPKYAYFN CCCCCCCCCCEEEEC	25.26	27097102
264	Ubiquitination	SKDCGSPKYAYFNGC CCCCCCCCEEEECCC	44.18	-
265	Phosphorylation	KDCGSPKYAYFNGCS CCCCCCCEEEECCCC	15.21	14681032
267	Phosphorylation	CGSPKYAYFNGCSSP CCCCCEEEECCCCCC	8.42	27097102
271	S-nitrosocysteine	KYAYFNGCSSPTAPL CEEEECCCCCCCCCC	3.69	-
271	S-nitrosylation	KYAYFNGCSSPTAPL CEEEECCCCCCCCCC	3.69	-
272	Phosphorylation	YAYFNGCSSPTAPLS EEEECCCCCCCCCCC	40.53	27097102
273	Phosphorylation	AYFNGCSSPTAPLSP EEECCCCCCCCCCCC	30.28	27097102
275	Phosphorylation	FNGCSSPTAPLSPMS ECCCCCCCCCCCCCC	42.74	27097102
279	Phosphorylation	SSPTAPLSPMSPPGY CCCCCCCCCCCCCCC	19.93	8631994
282	Phosphorylation	TAPLSPMSPPGYKLV CCCCCCCCCCCCEEC	31.18	8631994
286	Phosphorylation	SPMSPPGYKLVTGDR CCCCCCCCEECCCCC	13.61	27097102
287	Ubiquitination	PMSPPGYKLVTGDRN CCCCCCCEECCCCCC	42.25	-
290	Phosphorylation	PPGYKLVTGDRNNSS CCCCEECCCCCCCHH	43.74	23991683
296	Phosphorylation	VTGDRNNSSCRNYNK CCCCCCCHHCCCCCH	34.07	30411139
297	Phosphorylation	TGDRNNSSCRNYNKQ CCCCCCHHCCCCCHH	21.15	30411139
301	Phosphorylation	NNSSCRNYNKQASEQ CCHHCCCCCHHHHHC	12.37	22673903
303	Ubiquitination	SSCRNYNKQASEQNW HHCCCCCHHHHHCHH	36.56	-
306	Phosphorylation	RNYNKQASEQNWANY CCCCHHHHHCHHHHH	36.90	23991683
313	Phosphorylation	SEQNWANYSAEQNRM HHCHHHHHHHHHHCC	11.08	27097102
314	Phosphorylation	EQNWANYSAEQNRMG HCHHHHHHHHHHCCC	25.74	27097102
325	Phosphorylation	NRMGQAGSTISNSHA HCCCCCCCCCCCCCC	26.11	23991683
326	Phosphorylation	RMGQAGSTISNSHAQ CCCCCCCCCCCCCCC	27.75	30411139
328	Phosphorylation	GQAGSTISNSHAQPF CCCCCCCCCCCCCCC	32.16	23991683
330	Phosphorylation	AGSTISNSHAQPFDF CCCCCCCCCCCCCCC	17.26	23991683
364	Phosphorylation	AIVDQRPSSRASSRA EEECCCCCCCHHHCC	34.35	23712012
365	Phosphorylation	IVDQRPSSRASSRAS EECCCCCCCHHHCCC	34.25	23712012
368	Phosphorylation	QRPSSRASSRASSRP CCCCCCHHHCCCCCC	21.17	19423616
369	Phosphorylation	RPSSRASSRASSRPR CCCCCHHHCCCCCCC	30.77	23712012
372	Phosphorylation	SRASSRASSRPRPDD CCHHHCCCCCCCCCC	26.27	30411139
373	Phosphorylation	RASSRASSRPRPDDL CHHHCCCCCCCCCCC	45.37	23712012

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
244	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
244	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
247	Y	Phosphorylation	Kinase	SRC64	-	PhosphoELM
247	Y	Phosphorylation	Kinase	TYK2	P29597	PSP
247	Y	Phosphorylation	Kinase	SRC	P12931	PSP
255	S	Phosphorylation	Kinase	MAPK-FAMILY	-	GPS
255	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
255	S	Phosphorylation	Kinase	MAPK3	P21708	GPS
255	S	Phosphorylation	Kinase	MAPK7	Q13164	GPS
255	S	Phosphorylation	Kinase	MK07	P0C865	PhosphoELM
255	S	Phosphorylation	Kinase	MK01	P63086	PhosphoELM
255	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
255	S	Phosphorylation	Kinase	MAPK1	P63085	GPS
257	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
257	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
262	S	Phosphorylation	Kinase	PRKCA	P05696	GPS
262	S	Phosphorylation	Kinase	PKCE	P09216	PSP
265	Y	Phosphorylation	Kinase	SRC	Q9WUD9	PSP
265	Y	Phosphorylation	Kinase	SRC64	-	PhosphoELM
265	Y	Phosphorylation	Kinase	TYK2	P29597	PSP
265	Y	Phosphorylation	Kinase	SRC	P12931	PSP
267	Y	Phosphorylation	Kinase	TYK2	P29597	PSP
279	S	Phosphorylation	Kinase	MAPK3	P21708	GPS
279	S	Phosphorylation	Kinase	MAPK1	P63086	GPS
279	S	Phosphorylation	Kinase	ERK2	P63085	PSP
279	S	Phosphorylation	Kinase	MAPK-FAMILY	-	GPS
282	S	Phosphorylation	Kinase	ERK2	P63085	PSP
282	S	Phosphorylation	Kinase	MAPK-FAMILY	-	GPS
282	S	Phosphorylation	Kinase	MAPK1	P63086	GPS
282	S	Phosphorylation	Kinase	MAPK3	P21708	GPS
296	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
296	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
297	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
297	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
306	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
306	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
313	Y	Phosphorylation	Kinase	TYK2	P29597	PSP
314	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
314	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
325	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
325	S	Phosphorylation	Kinase	CSNK1D	Q06486	GPS
325	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
328	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
328	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
328	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
328	S	Phosphorylation	Kinase	CSNK1D	Q06486	GPS
330	S	Phosphorylation	Kinase	CSNK1D	Q06486	GPS
330	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
330	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
330	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
364	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
364	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
364	S	Phosphorylation	Kinase	PRKCE	Q02156	GPS
364	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
364	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
365	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
365	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
365	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
365	S	Phosphorylation	Kinase	PRKCE	Q02156	GPS
365	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
368	S	Phosphorylation	Kinase	PRKCG	P05129	GPS
368	S	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
368	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
368	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
368	S	Phosphorylation	Kinase	PKCA	P04409	PSP
368	S	Phosphorylation	Kinase	PKCA	P17252	PSP
368	S	Phosphorylation	Kinase	PRKCA	P20444	GPS
368	S	Phosphorylation	Kinase	PKCA	P05696	PSP
368	S	Phosphorylation	Kinase	PRKCG	P63319	Uniprot
368	S	Phosphorylation	Kinase	PRKCB	P05126-2	GPS
368	S	Phosphorylation	Kinase	PRKCD	P09215	Uniprot
368	S	Phosphorylation	Kinase	PKCE	P09216	PSP
368	S	Phosphorylation	Kinase	PRKCE	Q02156	GPS
369	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
369	S	Phosphorylation	Kinase	PKCA	P05696	PSP
369	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
369	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
369	S	Phosphorylation	Kinase	PRKCE	Q02156	GPS
369	S	Phosphorylation	Kinase	AKT1	P31749	PSP
369	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
372	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
372	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
372	S	Phosphorylation	Kinase	PRKCE	Q02156	GPS
372	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
373	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
373	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
373	S	Phosphorylation	Kinase	PRKCE	Q02156	GPS
373	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
373	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
373	S	Phosphorylation	Kinase	AKT3	Q63484	PSP
373	S	Phosphorylation	Kinase	AKT1	P31749	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
271	C	S-nitrosylation	-
S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.
325	S	Phosphorylation	22673903
Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly.
328	S	Phosphorylation	22673903
Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly.
330	S	Phosphorylation	22673903
Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly.
368	S	Phosphorylation	12417300
Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity).
368	S	Phosphorylation	12417300
Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CXA1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UBC_RAT	Ubc	physical	15371442
UBC_RAT	Ubc	physical	14970263
NEDD4_RAT	Nedd4	physical	16931598
NEDD4_RAT	Nedd4	physical	21936631
RLA2_RAT	Rplp2	physical	23106098
TMM43_RAT	Tmem43	physical	23106098
ACTS_RAT	Acta1	physical	23106098
PROF1_RAT	Pfn1	physical	23106098
PRDX2_RAT	Prdx2	physical	23106098
RS3A_RAT	Rps3a	physical	23106098
1433Z_RAT	Ywhaz	physical	23106098
ANXA1_RAT	Anxa1	physical	23106098
RL22_RAT	Rpl22	physical	23106098
ALDOA_RAT	Aldoa	physical	23106098
RL27_RAT	Rpl27	physical	23106098
RAP1A_RAT	Rap1a	physical	23106098
EF1D_RAT	Eef1d	physical	23106098
ALDR_RAT	Akr1b1	physical	23106098
ANXA2_RAT	Anxa2	physical	23106098
RLA0_RAT	Rplp0	physical	23106098
NFM_RAT	Nefm	physical	23106098
AT1B3_RAT	Atp1b3	physical	23106098
ATPB_RAT	Atp5b	physical	23106098
RAN_RAT	Ran	physical	23106098
PSD13_RAT	Psmd13	physical	23106098
VPS29_RAT	Vps29	physical	23106098
RL23_RAT	Rpl23	physical	23106098
ACTN4_RAT	Actn4	physical	23106098
OAT_RAT	Oat	physical	23106098
UN45A_RAT	Unc45a	physical	23106098
NUP93_RAT	Nup93	physical	23106098
RS17_RAT	Rps17	physical	23106098
PRDX1_RAT	Prdx1	physical	23106098
CLH1_RAT	Cltc	physical	23106098
PGS2_RAT	Dcn	physical	23106098
LEG1_RAT	Lgals1	physical	23106098
EF2_RAT	Eef2	physical	23106098
EF1G_RAT	Eef1g	physical	23106098
SODC_RAT	Sod1	physical	23106098
HS90A_RAT	Hsp90aa1	physical	23106098
VIME_RAT	Vim	physical	23106098
ARPC5_RAT	Arpc5	physical	23106098
NFL_RAT	Nefl	physical	23106098
NDUS1_RAT	Ndufs1	physical	23106098
EIF3E_RAT	Eif3e	physical	23106098
ARP2_RAT	Actr2	physical	23106098
RS16_RAT	Rps16	physical	23106098
CH60_RAT	Hspd1	physical	23106098
ACADL_RAT	Acadl	physical	23106098
ECHA_RAT	Hadha	physical	23106098
PDIA3_RAT	Pdia3	physical	23106098
KPYM_RAT	Pkm	physical	23106098
LMNB1_RAT	Lmnb1	physical	23106098
GDN_RAT	Serpine2	physical	23106098
COF1_RAT	Cfl1	physical	23106098
PPIA_RAT	Ppia	physical	23106098
RSSA_RAT	Rpsa	physical	23106098
SYDC_RAT	Dars	physical	23106098
H15_RAT	Hist1h1b	physical	23106098
TBA1A_RAT	Tuba1a	physical	23106098
UBB_RAT	Ubb	physical	23106098
CAZA2_RAT	Capza2	physical	23106098
TRXR1_RAT	Txnrd1	physical	23106098
CAZA1_RAT	Capza1	physical	23106098
GNAI2_RAT	Gnai2	physical	23106098
CATL1_RAT	Ctsl	physical	23106098
NRM_RAT	Nrm	physical	23106098
QCR2_RAT	Uqcrc2	physical	23106098
PRDX3_RAT	Prdx3	physical	23106098
G3P_RAT	Gapdh	physical	23106098
PLD3_RAT	Pld3	physical	23106098
HS90B_RAT	Hsp90ab1	physical	23106098
TBB4B_RAT	Tubb4b	physical	23106098
FLNC_RAT	Flnc	physical	23106098
HSP7C_RAT	Hspa8	physical	23106098
PRDX5_RAT	Prdx5	physical	23106098
HNRPD_RAT	Hnrpd	physical	23106098
PSB7_RAT	Psmb7	physical	23106098
PSMD2_RAT	Psmd2	physical	23106098
GRP78_RAT	Hspa5	physical	23106098
VDAC2_RAT	Vdac2	physical	23106098
ENOA_RAT	Eno1	physical	23106098
ADT2_RAT	Slc25a5	physical	23106098
ADT1_RAT	Slc25a4	physical	23106098
AT1A1_RAT	Atp1a1	physical	23106098
CXA1_RAT	Gja1	physical	23106098
ELP6_RAT	Elp6	physical	23106098
ELP5_RAT	Elp5	physical	23106098
DHB12_RAT	Hsd17b12	physical	23106098
NEDD4_RAT	Nedd4	physical	24236122
UBC_RAT	Ubc	physical	24236122
NEDD4_RAT	Nedd4	physical	25748165

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CXA1_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Identification and functional analysis of novel phosphorylation sitesin Cx43 in rat primary granulosa cells."; Yogo K., Ogawa T., Akiyama M., Ishida N., Takeya T.; FEBS Lett. 531:132-136(2002). Cited for: PHOSPHORYLATION AT SER-365; SER-368; SER-369 AND SER-373.	12417300 [Abstract]

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