dbPTM

NFL_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFL_RAT
UniProt AC	P19527
Protein Name	Neurofilament light polypeptide
Gene Name	Nefl
Organism	Rattus norvegicus (Rat).
Sequence Length	542
Subcellular Localization
Protein Description	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber..
Protein Sequence	MSSFSYEPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYSGLQSSSYLMSARAFPAYYTSHVQEEQSEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDAKEEEGGEGEEEDTKESEEEEKKEESAGEEQAAKKKD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSSFSYEPY ------CCCCCCCCC	39.84	22673903
2	Acetylation	------MSSFSYEPY ------CCCCCCCCC	39.84	-
3	Phosphorylation	-----MSSFSYEPYF -----CCCCCCCCCC	19.53	22673903
5	Phosphorylation	---MSSFSYEPYFST ---CCCCCCCCCCCC	30.45	22673903
6	Phosphorylation	--MSSFSYEPYFSTS --CCCCCCCCCCCCC	20.93	24972320
11	Phosphorylation	FSYEPYFSTSYKRRY CCCCCCCCCCCCCCC	15.59	22673903
12	Phosphorylation	SYEPYFSTSYKRRYV CCCCCCCCCCCCCCE	26.61	22673903
13	Phosphorylation	YEPYFSTSYKRRYVE CCCCCCCCCCCCCEE	27.71	22817900
15	Acetylation	PYFSTSYKRRYVETP CCCCCCCCCCCEECC	30.11	22902405
21	O-linked_Glycosylation	YKRRYVETPRVHISS CCCCCEECCCEEHHH	13.32	32574643
23	Asymmetric dimethylarginine	RRYVETPRVHISSVR CCCEECCCEEHHHCC	39.81	-
23	Methylation	RRYVETPRVHISSVR CCCEECCCEEHHHCC	39.81	-
27	O-linked_Glycosylation	ETPRVHISSVRSGYS ECCCEEHHHCCCCCC	14.11	32574643
30	Methylation	RVHISSVRSGYSTAR CEEHHHCCCCCCCHH	26.72	-
34	Phosphorylation	SSVRSGYSTARSAYS HHCCCCCCCHHHHHH	21.08	-
34	O-linked_Glycosylation	SSVRSGYSTARSAYS HHCCCCCCCHHHHHH	21.08	32574643
38	Phosphorylation	SGYSTARSAYSSYSA CCCCCHHHHHHCCCC	29.15	-
40	Phosphorylation	YSTARSAYSSYSAPV CCCHHHHHHCCCCCC	10.21	-
42	Phosphorylation	TARSAYSSYSAPVSS CHHHHHHCCCCCCCC	15.49	22817900
43	Phosphorylation	ARSAYSSYSAPVSSS HHHHHHCCCCCCCCC	11.69	-
48	Phosphorylation	SSYSAPVSSSLSVRR HCCCCCCCCCCEEEC	17.10	-
48	O-linked_Glycosylation	SSYSAPVSSSLSVRR HCCCCCCCCCCEEEC	17.10	8510543
50	Phosphorylation	YSAPVSSSLSVRRSY CCCCCCCCCEEECCC	19.70	22817900
52	Phosphorylation	APVSSSLSVRRSYSS CCCCCCCEEECCCCC	18.61	26022182
56	Phosphorylation	SSLSVRRSYSSSSGS CCCEEECCCCCCCCC	20.85	28551015
57	Phosphorylation	SLSVRRSYSSSSGSL CCEEECCCCCCCCCC	15.68	28551015
58	Phosphorylation	LSVRRSYSSSSGSLM CEEECCCCCCCCCCC	25.62	28551015
59	Phosphorylation	SVRRSYSSSSGSLMP EEECCCCCCCCCCCC	21.93	28551015
60	Phosphorylation	VRRSYSSSSGSLMPS EECCCCCCCCCCCCC	32.55	28551015
61	Phosphorylation	RRSYSSSSGSLMPSL ECCCCCCCCCCCCCC	34.06	28551015
63	Phosphorylation	SYSSSSGSLMPSLEN CCCCCCCCCCCCCCC	24.36	28551015
67	Phosphorylation	SSGSLMPSLENLDLS CCCCCCCCCCCCCHH	34.06	28551015
74	Phosphorylation	SLENLDLSQVAAISN CCCCCCHHHHHHHHH	23.44	-
91	Ubiquitination	KSIRTQEKAQLQDLN HHHHHHHHHHHHHHH	31.45	-
91	Acetylation	KSIRTQEKAQLQDLN HHHHHHHHHHHHHHH	31.45	22902405
103	Phosphorylation	DLNDRFASFIERVHE HHHHHHHHHHHHHHH	24.84	22673903
157	Acetylation	AEDATNEKQALQGER HHHCCCHHHHHHHHH	42.83	22902405
178	Phosphorylation	LRNLQARYEEEVLSR HHHHHHHHHHHHHCH	30.61	-
184	Phosphorylation	RYEEEVLSREDAEGR HHHHHHHCHHHHHHH	38.51	-
224	Acetylation	MDEIAFLKKVHEEEI HHHHHHHHHHCHHHH	46.42	22902405
225	Acetylation	DEIAFLKKVHEEEIA HHHHHHHHHCHHHHH	51.47	8264427
268	Acetylation	DIRAQYEKLAAKNMQ HHHHHHHHHHHHHCC	38.19	22902405
268	Ubiquitination	DIRAQYEKLAAKNMQ HHHHHHHHHHHHHCC	38.19	-
282	Acetylation	QNAEEWFKSRFTVLT CCHHHHHHHHHHHHC	41.62	22902405
289	Phosphorylation	KSRFTVLTESAAKNT HHHHHHHCHHHHCCC	24.45	22673903
291	Phosphorylation	RFTVLTESAAKNTDA HHHHHCHHHHCCCHH	28.43	22673903
294	Acetylation	VLTESAAKNTDAVRA HHCHHHHCCCHHHHH	61.16	22902405
303	Acetylation	TDAVRAAKDEVSESR CHHHHHHHHHHHHHH	54.13	22902405
340	Acetylation	QLQELEDKQNADISA HHHHHHHHHHCCHHH	35.76	22902405
354	Acetylation	AMQDTINKLENELRS HHHHHHHHHHHHHHH	54.01	22902405
363	Acetylation	ENELRSTKSEMARYL HHHHHHCHHHHHHHH	45.74	22902405
371	Acetylation	SEMARYLKEYQDLLN HHHHHHHHHHHHHHC	45.50	22902405
373	Phosphorylation	MARYLKEYQDLLNVK HHHHHHHHHHHHCHH	13.02	-
390	Phosphorylation	LDIEIAAYRKLLEGE HHHHHHHHHHHHCCC	10.16	-
392	Acetylation	IEIAAYRKLLEGEET HHHHHHHHHHCCCCC	44.76	72622455
392	Ubiquitination	IEIAAYRKLLEGEET HHHHHHHHHHCCCCC	44.76	-
413	Phosphorylation	VGSITSGYSQSSQVF CCCCCCCCCCCCCHH	11.87	-
425	Phosphorylation	QVFGRSAYSGLQSSS CHHCCHHHCCCCCHH	12.43	-
432	Phosphorylation	YSGLQSSSYLMSARA HCCCCCHHHHHHHCC	27.77	-
433	Phosphorylation	SGLQSSSYLMSARAF CCCCCHHHHHHHCCC	14.36	-
436	Phosphorylation	QSSSYLMSARAFPAY CCHHHHHHHCCCCHH	16.41	22817900
443	Phosphorylation	SARAFPAYYTSHVQE HHCCCCHHHHHCHHH	13.91	25403869
444	Phosphorylation	ARAFPAYYTSHVQEE HCCCCHHHHHCHHHH	12.13	25403869
445	Phosphorylation	RAFPAYYTSHVQEEQ CCCCHHHHHCHHHHH	10.32	22673903
446	Phosphorylation	AFPAYYTSHVQEEQS CCCHHHHHCHHHHHH	12.92	22673903
453	Phosphorylation	SHVQEEQSEVEETIE HCHHHHHHHHHHHHH	47.06	25403869
473	Phosphorylation	EAKDEPPSEGEAEEE HHCCCCCCCCCHHHH	69.79	30411139
503	Phosphorylation	EEAAKDESEDAKEEE HHHHHHHCHHHHHHC	51.41	22673903
519	Phosphorylation	GEGEEEDTKESEEEE CCCCCCCCHHHHHHH	39.74	22673903
522	Phosphorylation	EEEDTKESEEEEKKE CCCCCHHHHHHHHHH	52.44	22673903
527	Acetylation	KESEEEEKKEESAGE HHHHHHHHHHHHHHH	69.62	26302492
528	Acetylation	ESEEEEKKEESAGEE HHHHHHHHHHHHHHH	70.73	26302492
531	Phosphorylation	EEEKKEESAGEEQAA HHHHHHHHHHHHHHH	42.54	30411139
539	Acetylation	AGEEQAAKKKD---- HHHHHHHHHCC----	64.47	26302492
540	Acetylation	GEEQAAKKKD----- HHHHHHHHCC-----	58.40	26302492

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
3	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
3	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
13	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
13	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
42	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
42	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
44	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
50	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
50	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
56	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
63	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
436	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
436	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NFL_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFL_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
TSC2_RAT	Tsc2	physical	12226091
TSC1_RAT	Tsc1	physical	12226091

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFL_RAT

Related Literatures of Post-Translational Modification

O-linked Glycosylation
Reference	PubMed
"Glycosylation of mammalian neurofilaments. Localization of multipleO-linked N-acetylglucosamine moieties on neurofilament polypeptides Land M."; Dong D.L.-Y., Xu Z.-S., Chevrier M.R., Cotter R.J., Cleveland D.W.,Hart G.W.; J. Biol. Chem. 268:16679-16687(1993). Cited for: GLYCOSYLATION AT THR-21 AND SER-27.	8344946 [Abstract]