TSC1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TSC1_RAT
• UniProt AC: Q9Z136
• Protein Name: Hamartin
• Gene Name: Tsc1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1163
• Subcellular Localization: Cytoplasm . Membrane ; Peripheral membrane protein . At steady state found in association with membranes.
• Protein Description: In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. [PubMed: 16707451 Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (By similarity Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (By similarity Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (By similarity]
• Protein Sequence: MAQLANIGELLSMLDSSTLGVRDDVTTIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGDAVSHQLSACFPHRSADVTTSSYVDTQNSYGGATSTPSSTSRLMLFSTPGQLPQSLSSLSTRPLPEPLQASLWSPSAVCGMTTPPTSPGNVPADLSHPYSKAFGTTTGGKGTPSGTPATSPPPAPPCPQDDCAHGPASQASATPPRKEERADSSRPYLPRQQDVPSDRGLEDLPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEADPVAPRGGFDSPFYRDSLSGSQRKTHSAASGTQGFSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADASPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKAKGNPEEDCVPSTSPMEVLDRLLEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQQQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRSMIAELRVELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELESLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLQKLEEDRAEAAEAAEERLDCCTDGCSDSLLGHNEEAAGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPNQRFSSRWEPTMGEPSSSIPTTVGSLPSSKSFLGMKTRELFRNKSESQCDEDGMTMSSFSETLKTELGKDSAGMENKTPPSLDAPHPSSPSSDSMGQLHIMDYNETHHEHS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
22	Methylation	DSSTLGVRDDVTTIF HHHCCCCCCHHHHHH	32.39	26494717
345	Phosphorylation	LPEPLQASLWSPSAV CCCCCCHHCCCCCCC	19.87	27097102
348	Phosphorylation	PLQASLWSPSAVCGM CCCHHCCCCCCCCCC	17.80	27097102
350	Phosphorylation	QASLWSPSAVCGMTT CHHCCCCCCCCCCCC	28.02	27097102
356	Phosphorylation	PSAVCGMTTPPTSPG CCCCCCCCCCCCCCC	22.79	27097102
357	Phosphorylation	SAVCGMTTPPTSPGN CCCCCCCCCCCCCCC	20.55	27097102
360	Phosphorylation	CGMTTPPTSPGNVPA CCCCCCCCCCCCCCC	49.08	27097102
361	Phosphorylation	GMTTPPTSPGNVPAD CCCCCCCCCCCCCCC	36.26	27097102
370	Phosphorylation	GNVPADLSHPYSKAF CCCCCCCCCCCHHCC	23.98	27097102
373	Phosphorylation	PADLSHPYSKAFGTT CCCCCCCCHHCCCCC	19.85	27097102
374	Phosphorylation	ADLSHPYSKAFGTTT CCCCCCCHHCCCCCC	23.20	27097102
379	Phosphorylation	PYSKAFGTTTGGKGT CCHHCCCCCCCCCCC	18.09	27097102
380	Phosphorylation	YSKAFGTTTGGKGTP CHHCCCCCCCCCCCC	24.67	27097102
381	Phosphorylation	SKAFGTTTGGKGTPS HHCCCCCCCCCCCCC	43.72	27097102
386	Phosphorylation	TTTGGKGTPSGTPAT CCCCCCCCCCCCCCC	20.12	27097102
388	Phosphorylation	TGGKGTPSGTPATSP CCCCCCCCCCCCCCC	55.95	27097102
390	Phosphorylation	GKGTPSGTPATSPPP CCCCCCCCCCCCCCC	17.68	27097102
393	Phosphorylation	TPSGTPATSPPPAPP CCCCCCCCCCCCCCC	42.86	27097102
394	Phosphorylation	PSGTPATSPPPAPPC CCCCCCCCCCCCCCC	37.20	27097102
412	Phosphorylation	DCAHGPASQASATPP CCCCCCHHHCCCCCC	29.43	27097102
415	Phosphorylation	HGPASQASATPPRKE CCCHHHCCCCCCCHH	25.66	27097102
417	Phosphorylation	PASQASATPPRKEER CHHHCCCCCCCHHHC	31.19	27097102
487	Phosphorylation	AAISKELSEITTAEA HHHHHHHHHHHHCCC	28.22	-
505	Phosphorylation	APRGGFDSPFYRDSL CCCCCCCCCCCCCCC	18.04	27097102
508	Phosphorylation	GGFDSPFYRDSLSGS CCCCCCCCCCCCCCC	19.52	25575281
511	Phosphorylation	DSPFYRDSLSGSQRK CCCCCCCCCCCCCCC	18.55	25575281
513	Phosphorylation	PFYRDSLSGSQRKTH CCCCCCCCCCCCCCC	40.11	23984901
515	Phosphorylation	YRDSLSGSQRKTHSA CCCCCCCCCCCCCCC	24.36	23984901
519	Phosphorylation	LSGSQRKTHSAASGT CCCCCCCCCCCCCCC	24.21	28432305
521	Phosphorylation	GSQRKTHSAASGTQG CCCCCCCCCCCCCCC	30.66	28432305
524	Phosphorylation	RKTHSAASGTQGFSV CCCCCCCCCCCCCCC	41.93	28432305
526	Phosphorylation	THSAASGTQGFSVNP CCCCCCCCCCCCCCC	24.03	27097102
530	Phosphorylation	ASGTQGFSVNPEPLH CCCCCCCCCCCHHCC	28.01	27097102
547	Phosphorylation	LDKHGPDTPKQAFTP HHCCCCCCCCCCCCC	35.20	29779826
553	Phosphorylation	DTPKQAFTPIDPPSG CCCCCCCCCCCCCCC	23.50	27097102
559	Phosphorylation	FTPIDPPSGSADASP CCCCCCCCCCCCCCC	51.25	27097102
561	Phosphorylation	PIDPPSGSADASPAG CCCCCCCCCCCCCCC	27.58	27097102
565	Phosphorylation	PSGSADASPAGDRDR CCCCCCCCCCCCCCC	18.81	27097102
574	Phosphorylation	AGDRDRQTSLETSIL CCCCCCCCCCEEECC	35.85	25575281
575	Phosphorylation	GDRDRQTSLETSILT CCCCCCCCCEEECCC	18.83	25575281
578	Phosphorylation	DRQTSLETSILTPSP CCCCCCEEECCCCCC	26.76	25575281
579	Phosphorylation	RQTSLETSILTPSPC CCCCCEEECCCCCCC	13.26	25575281
582	Phosphorylation	SLETSILTPSPCKIP CCEEECCCCCCCCCC	21.76	25575281
584	Phosphorylation	ETSILTPSPCKIPPQ EEECCCCCCCCCCCC	37.38	28432305
595	Phosphorylation	IPPQRGVSFGSGQLP CCCCCCCCCCCCCCC	27.06	27097102
598	Phosphorylation	QRGVSFGSGQLPPYD CCCCCCCCCCCCCCC	22.73	23984901
673	Phosphorylation	RLSLPSKSVDWTHFG HCCCCCCCCCCCCCC	29.64	27097102
886	Acetylation	MMKTAYRKELEKNRS HHHHHHHHHHHHHHH	54.71	22902405
1035	Phosphorylation	ASCGGRVTGGSSSSS CCCCCCCCCCCCCCC	34.17	27097102
1038	Phosphorylation	GGRVTGGSSSSSSEL CCCCCCCCCCCCCCC	28.52	27097102
1039	Phosphorylation	GRVTGGSSSSSSELS CCCCCCCCCCCCCCC	37.20	27097102
1040	Phosphorylation	RVTGGSSSSSSELST CCCCCCCCCCCCCCC	35.62	27097102
1041	Phosphorylation	VTGGSSSSSSELSTP CCCCCCCCCCCCCCC	39.57	27097102
1042	Phosphorylation	TGGSSSSSSELSTPE CCCCCCCCCCCCCCC	29.76	27097102
1043	Phosphorylation	GGSSSSSSELSTPEK CCCCCCCCCCCCCCC	44.50	27097102
1046	Phosphorylation	SSSSSELSTPEKPPN CCCCCCCCCCCCCCC	37.55	27097102
1047	Phosphorylation	SSSSELSTPEKPPNQ CCCCCCCCCCCCCCC	48.36	27097102
1068	Phosphorylation	EPTMGEPSSSIPTTV CCCCCCCCCCCCCCC	32.20	25575281
1069	Phosphorylation	PTMGEPSSSIPTTVG CCCCCCCCCCCCCCC	42.90	25575281
1070	Phosphorylation	TMGEPSSSIPTTVGS CCCCCCCCCCCCCCC	36.60	25575281
1073	Phosphorylation	EPSSSIPTTVGSLPS CCCCCCCCCCCCCCC	32.23	27097102
1074	Phosphorylation	PSSSIPTTVGSLPSS CCCCCCCCCCCCCCC	19.95	28432305
1077	Phosphorylation	SIPTTVGSLPSSKSF CCCCCCCCCCCCCHH	32.54	27097102
1080	Phosphorylation	TTVGSLPSSKSFLGM CCCCCCCCCCHHCCH	56.54	28432305
1081	Phosphorylation	TVGSLPSSKSFLGMK CCCCCCCCCHHCCHH	30.31	28432305
1083	Phosphorylation	GSLPSSKSFLGMKTR CCCCCCCHHCCHHHH	27.87	25575281
1097	Phosphorylation	RELFRNKSESQCDED HHHHHCCCHHHCCCC	46.13	27097102
1099	Phosphorylation	LFRNKSESQCDEDGM HHHCCCHHHCCCCCC	43.11	27097102
1130	Phosphorylation	SAGMENKTPPSLDAP CCCCCCCCCCCCCCC	52.60	25575281
1133	Phosphorylation	MENKTPPSLDAPHPS CCCCCCCCCCCCCCC	39.83	25575281
1140	Phosphorylation	SLDAPHPSSPSSDSM CCCCCCCCCCCCCCC	52.42	25575281
1141	Phosphorylation	LDAPHPSSPSSDSMG CCCCCCCCCCCCCCC	33.00	25575281
1143	Phosphorylation	APHPSSPSSDSMGQL CCCCCCCCCCCCCCE	49.04	25575281
1144	Phosphorylation	PHPSSPSSDSMGQLH CCCCCCCCCCCCCEE	36.77	25575281
1146	Phosphorylation	PSSPSSDSMGQLHIM CCCCCCCCCCCEEEE	27.10	25575281
1155	Phosphorylation	GQLHIMDYNETHHEH CCEEEECCCCCCCCC	9.49	25575281
1158	Phosphorylation	HIMDYNETHHEHS-- EEECCCCCCCCCC--	25.54	25575281

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TSC1_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
505	S	Phosphorylation	Phosphorylation at Ser-505 does not affect interaction with TSC2.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TSC1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RENI_RAT	Ren	physical	10807585
TSC2_RAT	Tsc2	physical	10807585
BEX3_RAT	Ngfrap1	physical	17355907

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.