ACTS_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ACTS_RAT
• UniProt AC: P68136
• Protein Name: Actin, alpha skeletal muscle
• Gene Name: Acta1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 377
• Subcellular Localization: Cytoplasm, cytoskeleton.
• Protein Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells..
• Protein Sequence: MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MCDEDETTA ------CCCCCCCEE	8.68	-
3	Acetylation	-----MCDEDETTAL -----CCCCCCCEEE	66.15	-
35	Phosphorylation	APRAVFPSIVGRPRH CCCCCCHHHCCCCCC	20.59	5130775
46	Oxidation	RPRHQGVMVGMGQKD CCCCCCEEEECCCCC	2.49	-
49	Oxidation	HQGVMVGMGQKDSYV CCCEEEECCCCCCCC	3.32	-
52	Acetylation	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	174327
52	Ubiquitination	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	-
54	Phosphorylation	VGMGQKDSYVGDEAQ EECCCCCCCCCCHHH	28.84	25403869
54	O-linked_Glycosylation	VGMGQKDSYVGDEAQ EECCCCCCCCCCHHH	28.84	9548347
55	Phosphorylation	GMGQKDSYVGDEAQS ECCCCCCCCCCHHHC	20.70	4817623
62	Phosphorylation	YVGDEAQSKRGILTL CCCCHHHCCCCEEEE	31.22	29779826
63	Acetylation	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	174317
63	N6-malonyllysine	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	-
63	Malonylation	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	-
63	Ubiquitination	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	-
68	Phosphorylation	QSKRGILTLKYPIEH HCCCCEEEEECEECC	21.20	28826663
70	Acetylation	KRGILTLKYPIEHGI CCCEEEEECEECCCC	43.69	174315
71	Phosphorylation	RGILTLKYPIEHGII CCEEEEECEECCCCC	16.74	22673903
75	Methylation	TLKYPIEHGIITNWD EEECEECCCCCCCHH	33.21	-
79	Phosphorylation	PIEHGIITNWDDMEK EECCCCCCCHHHHHH	28.47	36748793
86	Acetylation	TNWDDMEKIWHHTFY CCHHHHHHHHHHHHH	44.45	174323
86	Methylation	TNWDDMEKIWHHTFY CCHHHHHHHHHHHHH	44.45	-
86	Ubiquitination	TNWDDMEKIWHHTFY CCHHHHHHHHHHHHH	44.45	-
91	Phosphorylation	MEKIWHHTFYNELRV HHHHHHHHHHCCCCC	18.22	9548383
93	Phosphorylation	KIWHHTFYNELRVAP HHHHHHHHCCCCCCC	14.64	174691
115	Acetylation	TEAPLNPKANREKMT CCCCCCCCCCHHHHH	58.16	30595959
128	Phosphorylation	MTQIMFETFNVPAMY HHHHHHHHCCHHHHH	14.83	21630457
135	Phosphorylation	TFNVPAMYVAIQAVL HCCHHHHHHHHHHHH	6.71	21630457
150	Phosphorylation	SLYASGRTTGIVLDS HHHHCCCCEEEEEEC	32.66	22673903
151	Phosphorylation	LYASGRTTGIVLDSG HHHCCCCEEEEEECC	24.45	22673903
157	O-linked_Glycosylation	TTGIVLDSGDGVTHN CEEEEEECCCCCCCC	34.32	10632789
157	Phosphorylation	TTGIVLDSGDGVTHN CEEEEEECCCCCCCC	34.32	-
168	Phosphorylation	VTHNVPIYEGYALPH CCCCCCEECCCCHHH	9.38	3840507
171	Phosphorylation	NVPIYEGYALPHAIM CCCEECCCCHHHHHH	7.94	15569651
188	Phosphorylation	DLAGRDLTDYLMKIL HCCCCCHHHHHHHHH	27.21	22673903
190	Phosphorylation	AGRDLTDYLMKILTE CCCCHHHHHHHHHHH	11.94	18876225
193	Ubiquitination	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	-
193	Acetylation	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	22646669
200	Phosphorylation	KILTERGYSFVTTAE HHHHHCCCCCEEHHC	12.73	174795
201	O-linked_Glycosylation	ILTERGYSFVTTAER HHHHCCCCCEEHHCH	18.96	5130783
201	Phosphorylation	ILTERGYSFVTTAER HHHHCCCCCEEHHCH	18.96	5130783
204	Phosphorylation	ERGYSFVTTAEREIV HCCCCCEEHHCHHHH	20.48	22673903
205	Phosphorylation	RGYSFVTTAEREIVR CCCCCEEHHCHHHHH	22.63	9548407
215	Acetylation	REIVRDIKEKLCYVA HHHHHHHHHHHCEEE	53.75	8276005
220	Phosphorylation	DIKEKLCYVALDFEN HHHHHHCEEEEECCH	10.13	174797
234	O-linked_Glycosylation	NEMATAASSSSLEKS HHHHHHCCCCCCCHH	28.43	10633201
234	Phosphorylation	NEMATAASSSSLEKS HHHHHHCCCCCCCHH	28.43	28551015
235	Phosphorylation	EMATAASSSSLEKSY HHHHHCCCCCCCHHE	21.15	28551015
236	Phosphorylation	MATAASSSSLEKSYE HHHHCCCCCCCHHEE	36.13	28551015
237	Phosphorylation	ATAASSSSLEKSYEL HHHCCCCCCCHHEEC	42.36	28551015
241	Phosphorylation	SSSSLEKSYELPDGQ CCCCCCHHEECCCCC	17.92	28689409
242	Phosphorylation	SSSLEKSYELPDGQV CCCCCHHEECCCCCE	32.33	22108457
251	Phosphorylation	LPDGQVITIGNERFR CCCCCEEEECCCEEC	24.79	46314633
296	Phosphorylation	IDIRKDLYANNVMSG CEECHHHHHCCCCCC	19.88	174185
317	Ubiquitination	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	-
317	Acetylation	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	174321
320	Phosphorylation	DRMQKEITALAPSTM HHHHHHHHHHCCCCE	19.40	3972893
325	Phosphorylation	EITALAPSTMKIKII HHHHHCCCCEEEEEE	35.21	9423901
325	O-linked_Glycosylation	EITALAPSTMKIKII HHHHHCCCCEEEEEE	35.21	9548443
326	Phosphorylation	ITALAPSTMKIKIIA HHHHCCCCEEEEEEC	22.79	27097102
328	Ubiquitination	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	-
328	Acetylation	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	174325
330	Ubiquitination	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	-
330	Acetylation	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	7629333
364	Phosphorylation	MWITKQEYDEAGPSI HCCHHHHHCCCCCCC	19.31	7330141
370	O-linked_Glycosylation	EYDEAGPSIVHRKCF HHCCCCCCCCCCCCC	36.00	10633213
370	Phosphorylation	EYDEAGPSIVHRKCF HHCCCCCCCCCCCCC	36.00	22673903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
35	S	Phosphorylation	Kinase	PRKACA	P00517	GPS
201	S	Phosphorylation	Kinase	PRKACA	P00517	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
46	M	Oxidation	Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization.	-
49	M	Oxidation	Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization.	-
75	H	Methylation	Methylated at His-75 by SETD3.	-
86	K	Methylation	Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ACTS_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of ACTS_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.