dbPTM

NFM_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFM_RAT
UniProt AC	P12839
Protein Name	Neurofilament medium polypeptide
Gene Name	Nefm
Organism	Rattus norvegicus (Rat).
Sequence Length	846
Subcellular Localization
Protein Description	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber..
Protein Sequence	MSYTLDSLGNPSAYRRVPTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLAYSSAMLSSAESSLDFSQSSSLLNGGSGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIHALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRAVRKDIEESSMVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLECHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFSGSITGPLYTHRQPSVTISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEDALTVIAEELAASAKEEKEEAEEKEEEPEVEKSPVKSPEAKEEEEGEKEEEEEGQEEEEEEDEGVKSDQAEEGGSEKEGSSEKDEGEQEEEGETEAEGEGEEAEAKEEKKTEGKVEEMAIKEEIKVEKPEKAKSPVPKSPVEEVKPKPEAKAGKDEQKEEEKVEEKKEVAKESPKEEKVEKKEEKPKDVPDKKKAESPVKEKAVEEMITITKSVKVSLEKDTKEEKPQQQEKVKEKAEEEGGSEEEVGDKSPQESKKEDIAINGEVEGKEEEEQETQEKGSGQEEEKGVVTNGLDVSPAEEKKGEDRSDDKVVVTKKVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQGD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSYTLDSLG ------CCCCCCCCC	27.16	22673903
2	Acetylation	------MSYTLDSLG ------CCCCCCCCC	27.16	-
3	Phosphorylation	-----MSYTLDSLGN -----CCCCCCCCCC	15.26	22673903
4	Phosphorylation	----MSYTLDSLGNP ----CCCCCCCCCCH	19.44	22673903
19	O-linked_Glycosylation	SAYRRVPTETRSSFS HHHCCCCCCCCCCCC	47.32	10563217
31	Phosphorylation	SFSRVSGSPSSGFRS CCCCCCCCCCCCCCC	17.69	30411139
33	O-linked_Glycosylation	SRVSGSPSSGFRSQS CCCCCCCCCCCCCCC	44.80	8344946
33	Phosphorylation	SRVSGSPSSGFRSQS CCCCCCCCCCCCCCC	44.80	22673903
34	Phosphorylation	RVSGSPSSGFRSQSW CCCCCCCCCCCCCCC	45.39	22673903
34	O-linked_Glycosylation	RVSGSPSSGFRSQSW CCCCCCCCCCCCCCC	45.39	8702840
43	Methylation	FRSQSWSRGSPSTVS CCCCCCCCCCCCCCC	43.27	-
45	Phosphorylation	SQSWSRGSPSTVSSS CCCCCCCCCCCCCHH	17.99	28432305
47	Phosphorylation	SWSRGSPSTVSSSYK CCCCCCCCCCCHHHH	43.04	25403869
47	O-linked_Glycosylation	SWSRGSPSTVSSSYK CCCCCCCCCCCHHHH	43.04	-
48	Phosphorylation	WSRGSPSTVSSSYKR CCCCCCCCCCHHHHH	28.00	28432305
48	O-linked_Glycosylation	WSRGSPSTVSSSYKR CCCCCCCCCCHHHHH	28.00	8702840
50	O-linked_Glycosylation	RGSPSTVSSSYKRSA CCCCCCCCHHHHHHC	17.41	-
50	Phosphorylation	RGSPSTVSSSYKRSA CCCCCCCCHHHHHHC	17.41	28432305
51	O-linked_Glycosylation	GSPSTVSSSYKRSAL CCCCCCCHHHHHHCH	33.09	-
51	Phosphorylation	GSPSTVSSSYKRSAL CCCCCCCHHHHHHCH	33.09	28432305
52	O-linked_Glycosylation	SPSTVSSSYKRSALA CCCCCCHHHHHHCHH	27.45	-
52	Phosphorylation	SPSTVSSSYKRSALA CCCCCCHHHHHHCHH	27.45	28432305
53	Phosphorylation	PSTVSSSYKRSALAP CCCCCHHHHHHCHHH	16.62	28432305
81	Phosphorylation	SSLDFSQSSSLLNGG HHCCHHHCCCCCCCC	22.24	16641100
83	Phosphorylation	LDFSQSSSLLNGGSG CCHHHCCCCCCCCCC	41.97	-
89	Phosphorylation	SSLLNGGSGGDYKLS CCCCCCCCCCCCCCC	40.79	-
93	Phosphorylation	NGGSGGDYKLSRSNE CCCCCCCCCCCCCCH	19.66	-
98	Phosphorylation	GDYKLSRSNEKEQLQ CCCCCCCCCHHHHHC	46.06	-
101	Acetylation	KLSRSNEKEQLQGLN CCCCCCHHHHHCCCH	55.82	22902405
126	Acetylation	HYLEQQNKEIEAEIH HHHHHHCHHHHHHHH	56.59	22902405
185	Acetylation	EEDIHRLKERFEEEA HHHHHHHHHHHHHHH	46.96	22902405
221	Acetylation	MVKVELDKKVQSLQD CEEEEHHHHHHHHHH	68.42	22902405
225	Phosphorylation	ELDKKVQSLQDEVAF EHHHHHHHHHHHHHH	30.87	-
270	Acetylation	TDISTALKEIRSQLE CCHHHHHHHHHHHHH	48.94	22902405
297	Acetylation	WFKCRYAKLTEAAEQ HHHHHHHHHHHHHHH	47.19	22902405
306	Acetylation	TEAAEQNKEAIRSAK HHHHHHCHHHHHHHH	48.49	22902405
313	Acetylation	KEAIRSAKEEIAEYR HHHHHHHHHHHHHHH	58.06	22902405
319	Phosphorylation	AKEEIAEYRRQLQSK HHHHHHHHHHHHHHC	11.32	-
327	Phosphorylation	RRQLQSKSIELESVR HHHHHHCCCCHHHHH	26.92	22673903
332	Phosphorylation	SKSIELESVRGTKES HCCCCHHHHHCCHHH	29.73	22673903
345	Phosphorylation	ESLERQLSDIEERHN HHHHHHHHHHHHHHC	28.36	25403869
358	Phosphorylation	HNHDLSSYQDTIQQL HCCCCHHHHHHHHHH	13.66	-
373	Acetylation	ENELRGTKWEMARHL HHHHHHCHHHHHHHH	43.94	22902405
383	Phosphorylation	MARHLREYQDLLNVK HHHHHHHHHHHHCHH	10.82	-
400	Phosphorylation	LDIEIAAYRKLLEGE HHHHHHHHHHHHCCC	10.16	-
402	Ubiquitination	IEIAAYRKLLEGEET HHHHHHHHHHCCCCC	44.76	-
402	Acetylation	IEIAAYRKLLEGEET HHHHHHHHHHCCCCC	44.76	72622461
415	Phosphorylation	ETRFSTFSGSITGPL CCCCCCCCCEECCCC	31.55	22673903
417	Phosphorylation	RFSTFSGSITGPLYT CCCCCCCEECCCCEE	18.77	22673903
419	Phosphorylation	STFSGSITGPLYTHR CCCCCEECCCCEECC	34.15	22673903
423	Phosphorylation	GSITGPLYTHRQPSV CEECCCCEECCCCCE	12.02	-
429	O-linked_Glycosylation	LYTHRQPSVTISSKI CEECCCCCEEECCCC	24.45	-
429	Phosphorylation	LYTHRQPSVTISSKI CEECCCCCEEECCCC	24.45	22673903
431	Phosphorylation	THRQPSVTISSKIQK ECCCCCEEECCCCCC	21.34	22673903
431	O-linked_Glycosylation	THRQPSVTISSKIQK ECCCCCEEECCCCCC	21.34	8702840
433	Phosphorylation	RQPSVTISSKIQKTK CCCCEEECCCCCCCC	19.06	22673903
434	Phosphorylation	QPSVTISSKIQKTKV CCCEEECCCCCCCCC	29.90	22673903
445	Acetylation	KTKVEAPKLKVQHKF CCCCCCCCCHHCHHH	68.91	22902405
467	Phosphorylation	TKVEDEKSEMEDALT HCCCCCHHHHHHHHH	39.88	22673903
483	Phosphorylation	IAEELAASAKEEKEE HHHHHHHHHHHHHHH	33.98	22673903
503	Phosphorylation	EEPEVEKSPVKSPEA CCCCHHCCCCCCHHH	22.78	30411139
507	Phosphorylation	VEKSPVKSPEAKEEE HHCCCCCCHHHHHHH	28.36	30240740
537	Phosphorylation	EEDEGVKSDQAEEGG HHHCCCCCHHHHCCC	32.33	22673903
545	Phosphorylation	DQAEEGGSEKEGSSE HHHHCCCCCCCCCCC	57.25	25403869
550	Phosphorylation	GGSEKEGSSEKDEGE CCCCCCCCCCCCCCC	36.09	28551015
551	Phosphorylation	GSEKEGSSEKDEGEQ CCCCCCCCCCCCCCC	60.57	30411139
564	Phosphorylation	EQEEEGETEAEGEGE CCCCCCCCCCCCCCH	51.88	22673903
604	Phosphorylation	EKPEKAKSPVPKSPV CCCHHCCCCCCCCCH	35.67	30411139
609	Phosphorylation	AKSPVPKSPVEEVKP CCCCCCCCCHHHCCC	28.27	30411139
617	Acetylation	PVEEVKPKPEAKAGK CHHHCCCCCCCCCCC	50.18	22902405
643	Phosphorylation	KKEVAKESPKEEKVE HHHHHHHCCCHHHCC	40.33	30240740
667	Phosphorylation	PDKKKAESPVKEKAV CCHHHCCCCCHHHHH	39.59	25403869
682	Ubiquitination	EEMITITKSVKVSLE HHHHHHHHHHEEEEC	49.77	-
685	Ubiquitination	ITITKSVKVSLEKDT HHHHHHHEEEECCCC	32.95	-
687	Phosphorylation	ITKSVKVSLEKDTKE HHHHHEEEECCCCCC	25.70	22673903
713	Phosphorylation	KAEEEGGSEEEVGDK HHHHHCCCHHHCCCC	53.33	30411139
721	Phosphorylation	EEEVGDKSPQESKKE HHHCCCCCCCHHHHH	36.23	30411139
725	Phosphorylation	GDKSPQESKKEDIAI CCCCCCHHHHHCCEE	43.72	30240740
751	Phosphorylation	QETQEKGSGQEEEKG HHHHHCCCCCHHHCC	48.66	25403869
761	Phosphorylation	EEEKGVVTNGLDVSP HHHCCEEECCCCCCH	22.59	28551015
767	Phosphorylation	VTNGLDVSPAEEKKG EECCCCCCHHHHHCC	20.33	30411139
790	Ubiquitination	VVTKKVEKITSEGGD EEEEEEEECCCCCCC	55.03	-
801	Acetylation	EGGDGATKYITKSVT CCCCCCCEEEEEEEE	33.84	22902405
840	Acetylation	VTSHAIVKEVTQGD- CCCCHHHEEHHCCC-	40.11	22902405

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
2	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
2	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
47	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
47	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
503	S	Phosphorylation	Kinase	GSK3A	P49840	PSP
507	S	Phosphorylation	Kinase	GSK3A	P49840	PSP
604	S	Phosphorylation	Kinase	GSK3A	P49840	PSP
604	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
609	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
667	S	Phosphorylation	Kinase	GSK3A	P49840	PSP
667	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
767	S	Phosphorylation	Kinase	PRKACA	P27791	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NFM_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFM_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of NFM_RAT !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFM_RAT

Related Literatures of Post-Translational Modification

O-linked Glycosylation
Reference	PubMed
"Glycosylation of mammalian neurofilaments. Localization of multipleO-linked N-acetylglucosamine moieties on neurofilament polypeptides Land M."; Dong D.L.-Y., Xu Z.-S., Chevrier M.R., Cotter R.J., Cleveland D.W.,Hart G.W.; J. Biol. Chem. 268:16679-16687(1993). Cited for: GLYCOSYLATION AT THR-48 AND THR-431.	8344946 [Abstract]
Phosphorylation
Reference	PubMed
"Identification of six phosphorylation sites in the COOH-terminal tailregion of the rat neurofilament protein M."; Xu Z.-S., Liu W.-S., Willard M.B.; J. Biol. Chem. 267:4467-4471(1992). Cited for: PHOSPHORYLATION AT SER-503; SER-507; SER-537; SER-604; SER-609 ANDSER-667, AND SEQUENCE REVISION TO 500.	1537832 [Abstract]