COF1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: COF1_RAT
• UniProt AC: P45592
• Protein Name: Cofilin-1
• Gene Name: Cfl1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 166
• Subcellular Localization: Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium .
• Protein Description: Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration (By similarity)..
• Protein Sequence: MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYTTFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MASGVAVSD ------CCCCEEECC	18.26	9877327
3	Phosphorylation	-----MASGVAVSDG -----CCCCEEECCC	32.28	23984901
8	Phosphorylation	MASGVAVSDGVIKVF CCCCEEECCCEEEEE	20.93	22673903
13	Acetylation	AVSDGVIKVFNDMKV EECCCEEEEECCCCC	38.07	22902405
19	Acetylation	IKVFNDMKVRKSSTP EEEECCCCCCCCCCH	41.57	134749
22	Succinylation	FNDMKVRKSSTPEEV ECCCCCCCCCCHHHH	52.31	26843850
23	Phosphorylation	NDMKVRKSSTPEEVK CCCCCCCCCCHHHHH	28.96	28432305
24	Phosphorylation	DMKVRKSSTPEEVKK CCCCCCCCCHHHHHH	51.52	28432305
25	Phosphorylation	MKVRKSSTPEEVKKR CCCCCCCCHHHHHHH	41.64	29779826
30	Acetylation	SSTPEEVKKRKKAVL CCCHHHHHHHHCEEE	50.57	22902405
41	Phosphorylation	KAVLFCLSEDKKNII CEEEEECCCCCCEEE	44.95	27097102
44	Acetylation	LFCLSEDKKNIILEE EEECCCCCCEEEECC	43.60	22902405
45	Ubiquitination	FCLSEDKKNIILEEG EECCCCCCEEEECCC	66.74	-
53	Ubiquitination	NIILEEGKEILVGDV EEEECCCCEEEECCC	44.88	-
68	Phosphorylation	GQTVDDPYTTFVKML CCCCCCCCCCHHEEC	25.96	-
70	Phosphorylation	TVDDPYTTFVKMLPD CCCCCCCCHHEECCC	22.16	-
73	Ubiquitination	DPYTTFVKMLPDKDC CCCCCHHEECCCCCC	30.45	-
73	Acetylation	DPYTTFVKMLPDKDC CCCCCHHEECCCCCC	30.45	-
78	Succinylation	FVKMLPDKDCRYALY HHEECCCCCCCHHEE	57.71	26843850
89	Phosphorylation	YALYDATYETKESKK HHEEECCCCCCCCCC	24.26	-
92	Ubiquitination	YDATYETKESKKEDL EECCCCCCCCCCCCE	48.31	-
92	Acetylation	YDATYETKESKKEDL EECCCCCCCCCCCCE	48.31	22902405
112	Ubiquitination	APESAPLKSKMIYAS CCCCCCCCCCEEEEC	47.15	-
114	Acetylation	ESAPLKSKMIYASSK CCCCCCCCEEEECCH	27.94	22648279
114	Ubiquitination	ESAPLKSKMIYASSK CCCCCCCCEEEECCH	27.94	-
121	Acetylation	KMIYASSKDAIKKKL CEEEECCHHHHHHHH	49.00	8273227
140	Phosphorylation	HELQANCYEEVKDRC HHHHHCHHHHHHHHC	18.22	21940666
144	Acetylation	ANCYEEVKDRCTLAE HCHHHHHHHHCHHHH	42.61	25786129
156	Phosphorylation	LAEKLGGSAVISLEG HHHHHCCCEEEEECC	19.98	21738781
160	Phosphorylation	LGGSAVISLEGKPL- HCCCEEEEECCEEC-	17.48	27097102

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
3	S	Phosphorylation	Kinase	ILK	Q13418	PSP
3	S	Phosphorylation	Kinase	LIMK1	P53667	PSP
3	S	Phosphorylation	Kinase	LIMK1	P53669	PSP
3	S	Phosphorylation	Kinase	LIMK2	P53671	PSP
3	S	Phosphorylation	Kinase	TESK2	Q924U5	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
3	S	Phosphorylation	Inactivated by phosphorylation on Ser-3.	9877327
3	S	Phosphorylation	Phosphorylated on Ser-3 in resting cells.	9877327
24	S	Phosphorylation	The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of COF1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AT1A1_RAT	Atp1a1	physical	11139403

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Complete amino acid sequences and phosphorylation sites, determinedby Edman degradation and mass spectrometry, of rat parotid destrin-and cofilin-like proteins.";
Kanamori T., Suzuki M., Titani K.;
Arch. Oral Biol. 43:955-967(1998).
Cited for: PROTEIN SEQUENCE OF 2-166, ACETYLATION AT ALA-2, PHOSPHORYLATION ATSER-3, AND MASS SPECTROMETRY.
PubMed: 9877327

Phosphorylation

"Complete amino acid sequences and phosphorylation sites, determinedby Edman degradation and mass spectrometry, of rat parotid destrin-and cofilin-like proteins.";
Kanamori T., Suzuki M., Titani K.;
Arch. Oral Biol. 43:955-967(1998).
Cited for: PROTEIN SEQUENCE OF 2-166, ACETYLATION AT ALA-2, PHOSPHORYLATION ATSER-3, AND MASS SPECTROMETRY.
PubMed: 9877327