NEDD4_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NEDD4_RAT
• UniProt AC: Q62940
• Protein Name: E3 ubiquitin-protein ligase NEDD4
• Gene Name: Nedd4
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 887
• Subcellular Localization: Cytoplasm . Cell membrane; Peripheral membrane protein. Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosome
• Protein Description: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (By similarity). Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Is involved in ubiquitination of ERBB4 intracellular domain E4ICD. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2 (By similarity). Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (By similarity)..
• Protein Sequence: MAADDTEAPVLSEDEVWEFCLDKNEEGGGSPGSDVTDTCEPPCGCWELNPSSLEEEHVLFTAESIISSFNNDDTRVVRVKVIAGIGLAKKDILGASDPYVRVTLYDPMSGVLTSVQTKTIKKSLNPKWNEEILFRVLPQQHRILFEVFDENRLTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSDDENADQAEELEPGWVVLDQPDAATHLQHPPEPSPLPPGWEERQDVLGRTYYVNHESRTTQWKRPSPEDDLTDDENGDIQLQAHGAFTTRRQISEDVDGPDNHESPENWEIVREDENTIYSGQAVQSPPSGHPDVQVRLAEELDTRLTMYGNPATSQPVTSSNHSSRGGSSQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTPVDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRMQNVAITGPAEPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATEDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEVVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSLNHQVIHWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
99	Phosphorylation	ILGASDPYVRVTLYD HCCCCCCEEEEEEEC	13.09	26022182
105	Phosphorylation	PYVRVTLYDPMSGVL CEEEEEEECCCCCCE	14.50	26022182
109	Phosphorylation	VTLYDPMSGVLTSVQ EEEECCCCCCEEEEE	31.01	26022182
113	Phosphorylation	DPMSGVLTSVQTKTI CCCCCCEEEEEEECC	25.17	26022182
114	Phosphorylation	PMSGVLTSVQTKTIK CCCCCEEEEEEECCH	14.20	26022182
212	Phosphorylation	TYLPKNGSDDENADQ EECCCCCCCCCCHHH	51.81	21630457
263	Phosphorylation	QDVLGRTYYVNHESR HCCCCCEEEECCCCC	12.21	-
278	Phosphorylation	TTQWKRPSPEDDLTD CCCCCCCCCCCCCCC	44.98	23712012
284	Phosphorylation	PSPEDDLTDDENGDI CCCCCCCCCCCCCCE	48.69	21630457
306	Phosphorylation	FTTRRQISEDVDGPD EECCCCCCCCCCCCC	21.29	27097102
317	Phosphorylation	DGPDNHESPENWEIV CCCCCCCCCCCCEEE	29.93	27097102
339	Phosphorylation	YSGQAVQSPPSGHPD ECCEECCCCCCCCCC	31.98	30240740
362	Phosphorylation	LDTRLTMYGNPATSQ HCCCEEECCCCCCCC	14.44	-
377	Phosphorylation	PVTSSNHSSRGGSSQ CCCCCCCCCCCCCCC	26.44	-
382	Phosphorylation	NHSSRGGSSQTCIFE CCCCCCCCCCEEEEC	23.30	23984901
383	Phosphorylation	HSSRGGSSQTCIFEE CCCCCCCCCEEEECC	32.55	23984901
385	Phosphorylation	SRGGSSQTCIFEEQP CCCCCCCEEEECCCC	14.98	25575281
393	Phosphorylation	CIFEEQPTLPVLLPT EEECCCCCCCEEEEC	43.31	25575281
543	Phosphorylation	ANILEDSYRRIMGVK HHHCHHHHHHHHCCC	19.07	-
571	Phosphorylation	DGEKGLDYGGVAREW CCCCCCCCCCHHHHH	22.33	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NEDD4_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NEDD4_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NEDD4_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KCNQ2_RAT	Kcnq2	physical	17322297
FOXJ2_HUMAN	FOXJ2	physical	19953087
PLS3_HUMAN	PLSCR3	physical	19953087
WBP2_HUMAN	WBP2	physical	19953087
ABL1_HUMAN	ABL1	physical	19953087
FGF12_HUMAN	FGF12	physical	19953087
FKBP3_HUMAN	FKBP3	physical	19953087
ANXA9_HUMAN	ANXA9	physical	19953087
IF5_HUMAN	EIF5	physical	19953087
MAPK5_HUMAN	MAPKAPK5	physical	19953087
TYY1_HUMAN	YY1	physical	19953087
MTAP2_HUMAN	MAP2	physical	19953087
M3K20_HUMAN	ZAK	physical	19953087
S23A2_HUMAN	SLC23A2	physical	19953087
BEAN1_HUMAN	BEAN1	physical	19953087
AKT3_HUMAN	AKT3	physical	19953087
AQP9_HUMAN	AQP9	physical	19953087
MAPK3_HUMAN	MAPKAPK3	physical	19953087
VGFR1_HUMAN	FLT1	physical	19953087
BRAF_HUMAN	BRAF	physical	19953087
TIM10_HUMAN	TIMM10	physical	19953087
HEP2_HUMAN	SERPIND1	physical	19953087
CUED1_HUMAN	CUEDC1	physical	19953087
KCC1D_HUMAN	CAMK1D	physical	19953087
SGK2_HUMAN	SGK2	physical	19953087
M3K3_HUMAN	MAP3K3	physical	19953087
TEC_HUMAN	TEC	physical	19953087
CDK5_HUMAN	CDK5	physical	19953087
AKT1_HUMAN	AKT1	physical	19953087
PGFRA_HUMAN	PDGFRA	physical	19953087
M3K8_HUMAN	MAP3K8	physical	19953087
TRPT1_HUMAN	TRPT1	physical	19953087
BLK_HUMAN	BLK	physical	19953087
KPCD_HUMAN	PRKCD	physical	19953087
FYN_HUMAN	FYN	physical	19953087
MP2K1_HUMAN	MAP2K1	physical	19953087
BMR1B_HUMAN	BMPR1B	physical	19953087
KTU_HUMAN	DNAAF2	physical	19953087
MK12_HUMAN	MAPK12	physical	19953087
RGS3_HUMAN	RGS3	physical	19953087
KLC4_HUMAN	KLC4	physical	19953087
NTRK2_HUMAN	NTRK2	physical	19953087
BTK_HUMAN	BTK	physical	19953087
EGFR_HUMAN	EGFR	physical	19953087
RPC4_HUMAN	POLR3D	physical	19953087
CSK21_HUMAN	CSNK2A1	physical	19953087
STK16_HUMAN	STK16	physical	19953087
ARBK1_HUMAN	ADRBK1	physical	19953087
DDX54_HUMAN	DDX54	physical	19953087
TRI41_HUMAN	TRIM41	physical	19953087
HMCES_HUMAN	HMCES	physical	19953087
TAF1B_HUMAN	TAF1B	physical	19953087
FGF21_HUMAN	FGF21	physical	19953087
STAC_HUMAN	STAC	physical	19953087
H10_HUMAN	H1F0	physical	19953087
ADAP2_HUMAN	ADAP2	physical	19953087
UBC12_HUMAN	UBE2M	physical	19953087
TMG1_HUMAN	PRRG1	physical	19953087
KCAB2_HUMAN	KCNAB2	physical	19953087
BMR1A_HUMAN	BMPR1A	physical	19953087
PPID_HUMAN	PPID	physical	19953087
RBX2_HUMAN	RNF7	physical	19953087
NHP2_HUMAN	NHP2	physical	19953087
SIVA_HUMAN	SIVA1	physical	19953087
RSLBB_HUMAN	RASL11B	physical	19953087
TIRR_HUMAN	NUDT16L1	physical	19953087
CG050_HUMAN	C7orf50	physical	19953087
TCAL2_HUMAN	TCEAL2	physical	19953087
MOB3A_HUMAN	MOB3A	physical	19953087
TCP1L_HUMAN	TCP10L	physical	19953087
CU077_HUMAN	TCP10L	physical	19953087
DIRA1_HUMAN	DIRAS1	physical	19953087
TEANC_HUMAN	TCEANC	physical	19953087
KCAB1_HUMAN	KCNAB1	physical	19953087
PTGR3_HUMAN	ZADH2	physical	19953087
PPARD_HUMAN	PPARD	physical	19953087
RNF37_HUMAN	UBOX5	physical	19953087
1433F_RAT	Ywhah	physical	15677482
1433B_RAT	Ywhab	physical	15677482
1433G_RAT	Ywhag	physical	15677482
1433E_RAT	Ywhae	physical	15677482
1433T_RAT	Ywhaq	physical	15677482
SCNNG_RAT	Scnn1g	physical	11805112
SCNNA_RAT	Scnn1a	physical	8665844
SCNNB_RAT	Scnn1b	physical	9923703
SCNNB_RAT	Scnn1b	physical	12654927
CXA1_RAT	Gja1	physical	26841867

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.