dbPTM

RBX2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RBX2_HUMAN
UniProt AC	Q9UBF6
Protein Name	RING-box protein 2
Gene Name	RNF7
Organism	Homo sapiens (Human).
Sequence Length	113
Subcellular Localization	Cytoplasm . Nucleus .
Protein Description	Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. [PubMed: 10851089 CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (By similarity Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.]
Protein Sequence	MADVEDGEETCALASHSGSSGSKSGGDKMFSLKKWNAVAMWSWDVECDTCAICRVQVMDACLRCQAENKQEDCVVVWGECNHSFHNCCMSLWVKQNNRCPLCQQDWVVQRIGK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MADVEDGEE ------CCCCCCCCE	29.56	19413330
10	Phosphorylation	DVEDGEETCALASHS CCCCCCEEEEEEECC	9.97	16874460
15	Phosphorylation	EETCALASHSGSSGS CEEEEEEECCCCCCC	21.02	27251275
17	Phosphorylation	TCALASHSGSSGSKS EEEEEECCCCCCCCC	37.56	25159151
19	Phosphorylation	ALASHSGSSGSKSGG EEEECCCCCCCCCCC	34.24	25159151
20	Phosphorylation	LASHSGSSGSKSGGD EEECCCCCCCCCCCC	51.08	25159151
22	Phosphorylation	SHSGSSGSKSGGDKM ECCCCCCCCCCCCCC	26.12	25627689
23	Ubiquitination	HSGSSGSKSGGDKMF CCCCCCCCCCCCCCE	57.56	-
28	Acetylation	GSKSGGDKMFSLKKW CCCCCCCCCEEECCC	45.15	23954790
28	Ubiquitination	GSKSGGDKMFSLKKW CCCCCCCCCEEECCC	45.15	29967540
31	O-linked_Glycosylation	SGGDKMFSLKKWNAV CCCCCCEEECCCCEE	35.46	30379171
31	Phosphorylation	SGGDKMFSLKKWNAV CCCCCCEEECCCCEE	35.46	-
58	Sulfoxidation	AICRVQVMDACLRCQ EEEEHHHHHHHHHHC	1.19	21406390

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
10	T	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
10	T	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
10	T	Phosphorylation	Kinase	CK2	-	Uniprot
10	T	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
-	K	Ubiquitination	E3 ubiquitin ligase	NEDD4	P46934	PMID:25216516

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RBX2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RBX2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CUL5_HUMAN	CUL5	physical	10230407
CUL1_HUMAN	CUL1	physical	10230407
CUL4A_HUMAN	CUL4A	physical	10230407
CUL2_HUMAN	CUL2	physical	10230407
ITM2C_HUMAN	ITM2C	physical	16189514
CUL1_HUMAN	CUL1	physical	11483504
FBW1A_HUMAN	BTRC	physical	20638939
CUL1_HUMAN	CUL1	physical	20638939
CUL1_HUMAN	CUL1	physical	11506706
SKP2_HUMAN	SKP2	physical	11255262
CSK2B_HUMAN	CSNK2B	physical	12470599
CSK21_HUMAN	CSNK2A1	physical	12470599
GLMN_HUMAN	GLMN	physical	22405651
RBX2_HUMAN	RNF7	physical	10443936
VHL_HUMAN	VHL	physical	17828303
CUL5_HUMAN	CUL5	physical	17828303
UB2D1_HUMAN	UBE2D1	physical	19037258
UB2R1_HUMAN	CDC34	physical	16874460
CHFR_HUMAN	CHFR	physical	22493164
UB2D1_HUMAN	UBE2D1	physical	24044920
NEDD4_HUMAN	NEDD4	physical	25216516
CUL5_HUMAN	CUL5	physical	25216516
UB2D1_HUMAN	UBE2D1	physical	20603330
CUL1_HUMAN	CUL1	physical	19250909
CUL2_HUMAN	CUL2	physical	19250909
CUL3_HUMAN	CUL3	physical	19250909
CUL4A_HUMAN	CUL4A	physical	19250909
CUL4B_HUMAN	CUL4B	physical	19250909
CUL5_HUMAN	CUL5	physical	19250909
UBE2F_HUMAN	UBE2F	physical	19250909
CUL5_HUMAN	CUL5	physical	23333304
PEBB_HUMAN	CBFB	physical	23333304
ELOC_HUMAN	TCEB1	physical	23333304
ELOB_HUMAN	TCEB2	physical	23333304
VIF_HV1B1	vif	physical	23333304
VIF_HV1BR	vif	physical	23333304
VIF_HV1H2	vif	physical	23333304
UBC12_HUMAN	UBE2M	physical	19250909
UB2D1_HUMAN	UBE2D1	physical	21737450
UB2D1_HUMAN	UBE2D1	physical	18799729
UB2R1_HUMAN	CDC34	physical	20638939
UB2D3_HUMAN	UBE2D3	physical	20638939
GHR_HUMAN	GHR	physical	25505247
UBE2F_HUMAN	UBE2F	physical	25912140
CUL5_HUMAN	CUL5	physical	25912140
SOCS3_HUMAN	SOCS3	physical	24438103
ELOB_HUMAN	TCEB2	physical	24438103
ELOC_HUMAN	TCEB1	physical	24438103
CUL5_HUMAN	CUL5	physical	24438103
UB2D1_HUMAN	UBE2D1	physical	24438103
UB2D2_HUMAN	UBE2D2	physical	24438103
UB2D3_HUMAN	UBE2D3	physical	24438103
UB2D3_HUMAN	UBE2D3	physical	27234298
UB2D1_HUMAN	UBE2D1	physical	27697924
CUL5_HUMAN	CUL5	physical	27910872
FBW1A_HUMAN	BTRC	physical	27910872
UBE2S_HUMAN	UBE2S	physical	27910872
UBE2C_HUMAN	UBE2C	physical	27910872
UB2D3_HUMAN	UBE2D3	physical	27910872
ERBIN_HUMAN	ERBB2IP	physical	27910872
UB2D1_HUMAN	UBE2D1	physical	28453520
CUL5_HUMAN	CUL5	physical	27591266
UBE2F_HUMAN	UBE2F	physical	27591266
APR_HUMAN	PMAIP1	physical	27591266
UBE2S_HUMAN	UBE2S	physical	27591266
UBE2C_HUMAN	UBE2C	physical	27591266

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RBX2_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.	19413330 [Abstract]
Phosphorylation
Reference	PubMed
"Phosphorylation of threonine 10 on CKBBP1/SAG/ROC2/Rbx2 by proteinkinase CKII promotes the degradation of IkappaBalpha and p27Kip1."; Kim Y.-S., Lee J.-Y., Son M.-Y., Park W., Bae Y.-S.; J. Biol. Chem. 278:28462-28469(2003). Cited for: PHOSPHORYLATION AT THR-10 BY CK2.	12748192 [Abstract]