1433B_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: 1433B_RAT
• UniProt AC: P35213
• Protein Name: 14-3-3 protein beta/alpha
• Gene Name: Ywhab
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 246
• Subcellular Localization: Cytoplasm . Melanosome .
• Protein Description: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13..
• Protein Sequence: MTMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICSDVLELLDKYLILNATHAESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESYKDSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1 (in isoform 2)	Acetylation	-	6.72	-
1	Acetylation	-------MTMDKSEL -------CCCCHHHH	6.72	-
2	Acetylation	------MTMDKSELV ------CCCCHHHHH	33.79	-
2	Phosphorylation	------MTMDKSELV ------CCCCHHHHH	33.79	23984901
5	Acetylation	---MTMDKSELVQKA ---CCCCHHHHHHHH	34.70	22902405
6	Phosphorylation	--MTMDKSELVQKAK --CCCCHHHHHHHHH	31.72	27097102
11	Acetylation	DKSELVQKAKLAEQA CHHHHHHHHHHHHHH	39.75	22902405
11	Ubiquitination	DKSELVQKAKLAEQA CHHHHHHHHHHHHHH	39.75	-
13	Acetylation	SELVQKAKLAEQAER HHHHHHHHHHHHHHH	56.09	22902405
13	Ubiquitination	SELVQKAKLAEQAER HHHHHHHHHHHHHHH	56.09	-
21	Phosphorylation	LAEQAERYDDMAAAM HHHHHHHHHHHHHHH	14.06	6852775
29	Ubiquitination	DDMAAAMKAVTEQGH HHHHHHHHHHHHHCH	34.67	-
32	Phosphorylation	AAAMKAVTEQGHELS HHHHHHHHHHCHHCC	27.70	23984901
47	Phosphorylation	NEERNLLSVAYKNVV HHHHHHHHHHHHHHH	14.10	27097102
50	Phosphorylation	RNLLSVAYKNVVGAR HHHHHHHHHHHHCCC	10.84	23984901
51	Ubiquitination	NLLSVAYKNVVGARR HHHHHHHHHHHCCCH	33.26	-
51	Acetylation	NLLSVAYKNVVGARR HHHHHHHHHHHCCCH	33.26	22902405
60	Phosphorylation	VVGARRSSWRVISSI HHCCCHHHHHHHHHH	19.65	13577399
65	Phosphorylation	RSSWRVISSIEQKTE HHHHHHHHHHHHHHH	23.54	25403869
66	Phosphorylation	SSWRVISSIEQKTER HHHHHHHHHHHHHHH	21.01	22673903
70	Acetylation	VISSIEQKTERNEKK HHHHHHHHHHHHHHH	39.29	22902405
70	Ubiquitination	VISSIEQKTERNEKK HHHHHHHHHHHHHHH	39.29	-
84	Nitrated tyrosine	KQQMGKEYREKIEAE HHHHHHHHHHHHHHH	27.79	-
84	Nitration	KQQMGKEYREKIEAE HHHHHHHHHHHHHHH	27.79	-
106	Nitrated tyrosine	VLELLDKYLILNATH HHHHHHHHHHHCCCC	9.91	-
106	Nitration	VLELLDKYLILNATH HHHHHHHHHHHCCCC	9.91	-
117	Acetylation	NATHAESKVFYLKMK CCCCCCCEEEEEEEC	28.06	-
122	Ubiquitination	ESKVFYLKMKGDYFR CCEEEEEEECCHHHH	27.47	-
122	Acetylation	ESKVFYLKMKGDYFR CCEEEEEEECCHHHH	27.47	7298887
124	Acetylation	KVFYLKMKGDYFRYL EEEEEEECCHHHHHH	47.22	22902405
132	Phosphorylation	GDYFRYLSEVASGDN CHHHHHHHHHHCCCC	22.53	10620507
136	Phosphorylation	RYLSEVASGDNKQTT HHHHHHHCCCCCCCC	52.43	28689409
140	Ubiquitination	EVASGDNKQTTVSNS HHHCCCCCCCCCCCH	54.48	-
143	Phosphorylation	SGDNKQTTVSNSQQA CCCCCCCCCCCHHHH	21.31	10620507
159	Acetylation	QEAFEISKKEMQPTH HHHHHHHHHHCCCCC	59.42	22902405
159	Ubiquitination	QEAFEISKKEMQPTH HHHHHHHHHHCCCCC	59.42	-
160	Ubiquitination	EAFEISKKEMQPTHP HHHHHHHHHCCCCCC	52.18	-
186	Phosphorylation	FYYEILNSPEKACSL HHHHHHCCHHHHHHH	30.87	30240740
189	Acetylation	EILNSPEKACSLAKT HHHCCHHHHHHHHHH	59.06	25786129
192	Phosphorylation	NSPEKACSLAKTAFD CCHHHHHHHHHHHHH	35.90	29779826
207	Phosphorylation	EAIAELDTLNEESYK HHHHHHHCCCHHHCC	44.07	30181290
212	Phosphorylation	LDTLNEESYKDSTLI HHCCCHHHCCCHHHH	31.16	30181290
216	Phosphorylation	NEESYKDSTLIMQLL CHHHCCCHHHHHHHH	22.44	22673903
217	Phosphorylation	EESYKDSTLIMQLLR HHHCCCHHHHHHHHH	30.36	22673903
232	Phosphorylation	DNLTLWTSENQGDEG HCCEEEECCCCCCCC	23.98	24259510

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
60	S	Phosphorylation	Kinase	CAMK2A	P11275	PSP
65	S	Phosphorylation	Kinase	CAMK2A	P11275	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
60	S	Phosphorylation	Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of 1433B_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SYUA_RAT	Snca	physical	10407019

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.