dbPTM

KPYM_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	KPYM_RAT
UniProt AC	P11980
Protein Name	Pyruvate kinase PKM
Gene Name	Pkm
Organism	Rattus norvegicus (Rat).
Sequence Length	531
Subcellular Localization	Cytoplasm . Nucleus . Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
Protein Description	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation (By similarity)..
Protein Sequence	MPKPDSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRAATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADYLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAVFHRLLFEELARASSQSTDPLEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLDAWAEDVDLRVNLAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	"N6,N6,N6-trimethyllysine"	-----MPKPDSEAGT -----CCCCCCCCCC	61.22	-
3	Methylation	-----MPKPDSEAGT -----CCCCCCCCCC	61.22	-
37	Phosphorylation	MCRLDIDSAPITARN HHCCCCCCCCCEECC	35.96	-
41	Phosphorylation	DIDSAPITARNTGII CCCCCCCEECCCEEE	20.64	22673903
45	Phosphorylation	APITARNTGIICTIG CCCEECCCEEEEEEC	24.97	23984901
49	S-nitrosocysteine	ARNTGIICTIGPASR ECCCEEEEEECCCHH	1.83	-
49	S-nitrosylation	ARNTGIICTIGPASR ECCCEEEEEECCCHH	1.83	22178444
50	Phosphorylation	RNTGIICTIGPASRS CCCEEEEEECCCHHH	20.68	23984901
55	Phosphorylation	ICTIGPASRSVEMLK EEEECCCHHHHHHHH	28.37	23984901
57	Phosphorylation	TIGPASRSVEMLKEM EECCCHHHHHHHHHH	21.67	22673903
62	Acetylation	SRSVEMLKEMIKSGM HHHHHHHHHHHHCCC	43.88	22902405
66	Acetylation	EMLKEMIKSGMNVAR HHHHHHHHCCCCEEE	39.38	22902405
66	Succinylation	EMLKEMIKSGMNVAR HHHHHHHHCCCCEEE	39.38	-
66	Succinylation	EMLKEMIKSGMNVAR HHHHHHHHCCCCEEE	39.38	-
77	Phosphorylation	NVARLNFSHGTHEYH CEEEEECCCCCCCCC	21.67	28432305
80	Phosphorylation	RLNFSHGTHEYHAET EEECCCCCCCCCHHH	13.20	22673903
83	Phosphorylation	FSHGTHEYHAETIKN CCCCCCCCCHHHHHH	9.95	-
83	Nitration	FSHGTHEYHAETIKN CCCCCCCCCHHHHHH	9.95	-
89	Acetylation	EYHAETIKNVRAATE CCCHHHHHHHHHHHH	57.73	22902405
95	Phosphorylation	IKNVRAATESFASDP HHHHHHHHHHHCCCC	31.04	23984901
97	Phosphorylation	NVRAATESFASDPIL HHHHHHHHHCCCCCC	23.04	28432305
100	Phosphorylation	AATESFASDPILYRP HHHHHHCCCCCCCCC	41.74	22108457
105	Phosphorylation	FASDPILYRPVAVAL HCCCCCCCCCEEEEE	17.37	23984901
115	Acetylation	VAVALDTKGPEIRTG EEEEECCCCCCCCCE	72.62	22902405
121	Phosphorylation	TKGPEIRTGLIKGSG CCCCCCCCEEECCCC	41.84	22673903
127	Phosphorylation	RTGLIKGSGTAEVEL CCEEECCCCEEEEEE	28.15	25403869
129	Phosphorylation	GLIKGSGTAEVELKK EEECCCCEEEEEECC	22.72	25403869
135	Ubiquitination	GTAEVELKKGATLKI CEEEEEECCCCEEEE	35.34	-
135	Acetylation	GTAEVELKKGATLKI CEEEEEECCCCEEEE	35.34	22902405
141	Ubiquitination	LKKGATLKITLDNAY ECCCCEEEEEECCHH	29.32	-
143	Phosphorylation	KGATLKITLDNAYME CCCEEEEEECCHHHH	26.86	22673903
148	Nitration	KITLDNAYMEKCDEN EEEECCHHHHHCCCC	16.44	-
148	Phosphorylation	KITLDNAYMEKCDEN EEEECCHHHHHCCCC	16.44	22673903
166	Succinylation	LDYKNICKVVEVGSK ECHHCCEEEEEECCE	45.58	-
166	Succinylation	LDYKNICKVVEVGSK ECHHCCEEEEEECCE	45.58	-
166	Ubiquitination	LDYKNICKVVEVGSK ECHHCCEEEEEECCE	45.58	-
166	Acetylation	LDYKNICKVVEVGSK ECHHCCEEEEEECCE	45.58	22902405
175	Phosphorylation	VEVGSKIYVDDGLIS EEECCEEEEECCEEE	11.10	22673903
175	Nitration	VEVGSKIYVDDGLIS EEECCEEEEECCEEE	11.10	-
186	Acetylation	GLISLQVKEKGADYL CEEEEEEECCCCCEE	40.52	22902405
186	Ubiquitination	GLISLQVKEKGADYL CEEEEEEECCCCCEE	40.52	-
188	Acetylation	ISLQVKEKGADYLVT EEEEEECCCCCEEEE	54.39	22902405
192	Phosphorylation	VKEKGADYLVTEVEN EECCCCCEEEEEEEC	11.53	22673903
192	Nitration	VKEKGADYLVTEVEN EECCCCCEEEEEEEC	11.53	-
195	Phosphorylation	KGADYLVTEVENGGS CCCCEEEEEEECCCC	31.55	22673903
202	Phosphorylation	TEVENGGSLGSKKGV EEEECCCCCCCCCCC	30.84	22673903
205	Phosphorylation	ENGGSLGSKKGVNLP ECCCCCCCCCCCCCC	36.17	23984901
206	Acetylation	NGGSLGSKKGVNLPG CCCCCCCCCCCCCCC	52.31	22902405
206	Ubiquitination	NGGSLGSKKGVNLPG CCCCCCCCCCCCCCC	52.31	-
207	Ubiquitination	GGSLGSKKGVNLPGA CCCCCCCCCCCCCCC	70.55	-
222	Phosphorylation	AVDLPAVSEKDIQDL CCCCCCCCHHHHHHH	40.82	22673903
224	Acetylation	DLPAVSEKDIQDLKF CCCCCCHHHHHHHCC	53.26	22902405
266	Ubiquitination	GEKGKNIKIISKIEN CCCCCCCEEEEEECC	43.30	-
266	Acetylation	GEKGKNIKIISKIEN CCCCCCCEEEEEECC	43.30	22902405
270	Acetylation	KNIKIISKIENHEGV CCCEEEEEECCCHHH	43.56	22902405
305	Acetylation	GIEIPAEKVFLAQKM CCCCCHHHHHHHHHH	40.15	22902405
311	Acetylation	EKVFLAQKMMIGRCN HHHHHHHHHHHCCCC	25.48	22902405
322	Succinylation	GRCNRAGKPVICATQ CCCCCCCCCEEEHHH	34.82	-
322	Succinylation	GRCNRAGKPVICATQ CCCCCCCCCEEEHHH	34.82	-
322	Acetylation	GRCNRAGKPVICATQ CCCCCCCCCEEEHHH	34.82	-
326	S-nitrosocysteine	RAGKPVICATQMLES CCCCCEEEHHHHHHH	3.23	-
326	S-nitrosylation	RAGKPVICATQMLES CCCCCEEEHHHHHHH	3.23	22178444
358	S-nitrosylation	AVLDGADCIMLSGET HHHCCCCEEEEECCC	1.63	22178444
358	S-nitrosocysteine	AVLDGADCIMLSGET HHHCCCCEEEEECCC	1.63	-
370	Phosphorylation	GETAKGDYPLEAVRM CCCCCCCCCHHHHHH	20.56	28432305
370	Nitration	GETAKGDYPLEAVRM CCCCCCCCCHHHHHH	20.56	-
408	Hydroxylation	ASSQSTDPLEAMAMG HHCCCCCHHHHHHHC	31.99	-
423	S-nitrosylation	SVEASYKCLAAALIV CHHHHHHHHHHHHHH	2.07	22178444
423	S-nitrosocysteine	SVEASYKCLAAALIV CHHHHHHHHHHHHHH	2.07	-
437	Phosphorylation	VLTESGRSAHQVARY HHCCCCCCHHHHHHH	33.36	25403869
474	S-nitrosylation	RGIFPVLCKDAVLDA CCHHHHHCCHHHHHH	3.69	22178444
474	S-nitrosocysteine	RGIFPVLCKDAVLDA CCHHHHHCCHHHHHH	3.69	-
475	Acetylation	GIFPVLCKDAVLDAW CHHHHHCCHHHHHHH	44.56	-
498	Succinylation	NLAMNVGKARGFFKK HHHHHCHHHCCCEEC	30.54	-
498	Succinylation	NLAMNVGKARGFFKK HHHHHCHHHCCCEEC	30.54	-
519	Phosphorylation	LTGWRPGSGFTNTMR ECCCCCCCCCCCCEE	33.16	22673903
522	Phosphorylation	WRPGSGFTNTMRVVP CCCCCCCCCCEEEEE	33.05	23984901
524	Phosphorylation	PGSGFTNTMRVVPVP CCCCCCCCEEEEECC	11.87	23984901

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of KPYM_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
305	K	Acetylation		-
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of KPYM_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of KPYM_RAT !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of KPYM_RAT

Related Literatures of Post-Translational Modification

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