| UniProt ID | RAD3_YEAST | |
|---|---|---|
| UniProt AC | P06839 | |
| Protein Name | General transcription and DNA repair factor IIH helicase subunit XPD | |
| Gene Name | RAD3 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 778 | |
| Subcellular Localization | Nucleus. | |
| Protein Description | ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/RAD3 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription. XPD/RAD3 acts by forming a bridge between TFIIK and the core-TFIIH complex. Involved in the maintenance of the fidelity of DNA replication.. | |
| Protein Sequence | MKFYIDDLPVLFPYPKIYPEQYNYMCDIKKTLDVGGNSILEMPSGTGKTVSLLSLTIAYQMHYPEHRKIIYCSRTMSEIEKALVELENLMDYRTKELGYQEDFRGLGLTSRKNLCLHPEVSKERKGTVVDEKCRRMTNGQAKRKLEEDPEANVELCEYHENLYNIEVEDYLPKGVFSFEKLLKYCEEKTLCPYFIVRRMISLCNIIIYSYHYLLDPKIAERVSNEVSKDSIVIFDEAHNIDNVCIESLSLDLTTDALRRATRGANALDERISEVRKVDSQKLQDEYEKLVQGLHSADILTDQEEPFVETPVLPQDLLTEAIPGNIRRAEHFVSFLKRLIEYLKTRMKVLHVISETPKSFLQHLKQLTFIERKPLRFCSERLSLLVRTLEVTEVEDFTALKDIATFATLISTYEEGFLLIIEPYEIENAAVPNPIMRFTCLDASIAIKPVFERFSSVIITSGTISPLDMYPRMLNFKTVLQKSYAMTLAKKSFLPMIITKGSDQVAISSRFEIRNDPSIVRNYGSMLVEFAKITPDGMVVFFPSYLYMESIVSMWQTMGILDEVWKHKLILVETPDAQETSLALETYRKACSNGRGAILLSVARGKVSEGIDFDHQYGRTVLMIGIPFQYTESRILKARLEFMRENYRIRENDFLSFDAMRHAAQCLGRVLRGKDDYGVMVLADRRFSRKRSQLPKWIAQGLSDADLNLSTDMAISNTKQFLRTMAQPTDPKDQEGVSVWSYEDLIKHQNSRKDQGGFIENENKEGEQDEDEDEDIEMQ | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 94 | Phosphorylation | ENLMDYRTKELGYQE HHHHHHHHHHCCCCC | 23.73 | 30377154 | |
| 112 | Ubiquitination | GLGLTSRKNLCLHPE CCCCCCCCCEECCCC | 54.78 | 17644757 | |
| 122 | Ubiquitination | CLHPEVSKERKGTVV ECCCCCCCCCCCCCC | 67.71 | 17644757 | |
| 125 | Ubiquitination | PEVSKERKGTVVDEK CCCCCCCCCCCCCHH | 61.09 | 17644757 | |
| 180 | Acetylation | KGVFSFEKLLKYCEE CCCCCHHHHHHHHHH | 58.05 | 24489116 | |
| 223 | Phosphorylation | PKIAERVSNEVSKDS HHHHHHHCCCCCCCC | 33.23 | 30377154 | |
| 295 | Phosphorylation | KLVQGLHSADILTDQ HHHHHHHHCCCCCCC | 32.45 | 30377154 | |
| 347 | Ubiquitination | EYLKTRMKVLHVISE HHHHHHHHHHHHHCC | 38.05 | 17644757 | |
| 357 | Ubiquitination | HVISETPKSFLQHLK HHHCCCCHHHHHHHH | 62.35 | 17644757 | |
| 477 | Phosphorylation | PRMLNFKTVLQKSYA HHHHCHHHHHHHHHH | 23.47 | 19823750 | |
| 482 | Phosphorylation | FKTVLQKSYAMTLAK HHHHHHHHHHHHHHH | 12.74 | 19823750 | |
| 483 | Phosphorylation | KTVLQKSYAMTLAKK HHHHHHHHHHHHHHH | 13.87 | 19823750 | |
| 486 | Phosphorylation | LQKSYAMTLAKKSFL HHHHHHHHHHHHHCC | 18.52 | 19779198 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of RAD3_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of RAD3_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of RAD3_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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