dbPTM

KSR1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	KSR1_HUMAN
UniProt AC	Q8IVT5
Protein Name	Kinase suppressor of Ras 1
Gene Name	KSR1
Organism	Homo sapiens (Human).
Sequence Length	923
Subcellular Localization	Cytoplasm . Membrane Peripheral membrane protein . Cell membrane Peripheral membrane protein . Cell projection, ruffle membrane . Endoplasmic reticulum membrane . In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein,
Protein Description	Scaffolding protein that is part of a multiprotein signaling complex. Promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Does not have kinase activity by itself..
Protein Sequence	MDRAALRAAAMGEKKEGGGGGDAAAAEGGAGAAASRALQQCGQLQKLIDISIGSLRGLRTKCAVSNDLTQQEIRTLEAKLVRYICKQRQCKLSVAPGERTPELNSYPRFSDWLYTFNVRPEVVQEIPRDLTLDALLEMNEAKVKETLRRCGASGDECGRLQYALTCLRKVTGLGGEHKEDSSWSSLDARRESGSGPSTDTLSAASLPWPPGSSQLGRAGNSAQGPRSISVSALPASDSPTPSFSEGLSDTCIPLHASGRLTPRALHSFITPPTTPQLRRHTKLKPPRTPPPPSRKVFQLLPSFPTLTRSKSHESQLGNRIDDVSSMRFDLSHGSPQMVRRDIGLSVTHRFSTKSWLSQVCHVCQKSMIFGVKCKHCRLKCHNKCTKEAPACRISFLPLTRLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKEHPPAMNHLDSSSNPSSTTSSTPSSPAPFPTSSNPSSATTPPNPSPGQRDSRFNFPAAYFIHHRQQFIFPVPSAGHCWKCLLIAESLKENAFNISAFAHAAPLPEAADGTRLDDQPKADVLEAHEAEAEEPEAGKSEAEDDEDEVDDLPSSRRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDNGKVVITDFGLFGISGVVREGRRENQLKLSHDWLCYLAPEIVREMTPGKDEDQLPFSKAADVYAFGTVWYELQARDWPLKNQAAEASIWQIGSGEGMKRVLTSVSLGKEVSEILSACWAFDLQERPSFSLLMDMLEKLPKLNRRLSHPGHFWKSADINSSKVVPRFERFGLGVLESSNPKM

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
32	Ubiquitination	AAEGGAGAAASRALQ HHCCCHHHHHHHHHH	10.06	29967540
51	Phosphorylation	LQKLIDISIGSLRGL HHHHHHCCHHHHHCH	17.52	27174698
54	Phosphorylation	LIDISIGSLRGLRTK HHHCCHHHHHCHHHH	43.74	24719451
57	Phosphorylation	ISIGSLRGLRTKCAV CCHHHHHCHHHHHHH	34.66	24719451
60	Phosphorylation	GSLRGLRTKCAVSND HHHHCHHHHHHHCCC	4.46	27174698
61	Phosphorylation	SLRGLRTKCAVSNDL HHHCHHHHHHHCCCC	14.88	27251275
90	Phosphorylation	YICKQRQCKLSVAPG HHHHHCCCCCEECCC	7.00	27251275
103	Phosphorylation	PGERTPELNSYPRFS CCCCCCCCCCCCCCC	49.49	27251275
111	Phosphorylation	NSYPRFSDWLYTFNV CCCCCCCCCCCCCCC	3.28	33259812
120	Phosphorylation	LYTFNVRPEVVQEIP CCCCCCCHHHHHHCC	6.28	27251275
133	Phosphorylation	IPRDLTLDALLEMNE CCCCCCHHHHHHCCH	4.09	18669648
136	Phosphorylation	DLTLDALLEMNEAKV CCCHHHHHHCCHHHH	5.76	32142685
137	Phosphorylation	LTLDALLEMNEAKVK CCHHHHHHCCHHHHH	36.38	24719451
151	Phosphorylation	KETLRRCGASGDECG HHHHHHHCCCCCHHH	31.87	24719451
162	Phosphorylation	DECGRLQYALTCLRK CHHHHHHHHHHHHHH	2.71	-
174	Phosphorylation	LRKVTGLGGEHKEDS HHHHHCCCCCCCCCC	50.41	9858547
182	Phosphorylation	GEHKEDSSWSSLDAR CCCCCCCCCCCHHHH	23.99	28555341
184	Phosphorylation	HKEDSSWSSLDARRE CCCCCCCCCHHHHHH	5.08	-
192	Phosphorylation	SLDARRESGSGPSTD CHHHHHHCCCCCCCC	57.73	26657352
194	Phosphorylation	DARRESGSGPSTDTL HHHHHCCCCCCCCCC	35.43	23403867
197	Phosphorylation	RESGSGPSTDTLSAA HHCCCCCCCCCCCCC	50.41	23401153
198	Phosphorylation	ESGSGPSTDTLSAAS HCCCCCCCCCCCCCC	23.77	23403867
200	Phosphorylation	GSGPSTDTLSAASLP CCCCCCCCCCCCCCC	24.71	23403867
202	Phosphorylation	GPSTDTLSAASLPWP CCCCCCCCCCCCCCC	14.28	23403867
205	Phosphorylation	TDTLSAASLPWPPGS CCCCCCCCCCCCCCC	38.75	23403867
212	Phosphorylation	SLPWPPGSSQLGRAG CCCCCCCCCCCCCCC	50.03	25262027
213	Phosphorylation	LPWPPGSSQLGRAGN CCCCCCCCCCCCCCC	5.61	25262027
227	Phosphorylation	NSAQGPRSISVSALP CCCCCCCEEEEEECC	15.97	28857561
229	Phosphorylation	AQGPRSISVSALPAS CCCCCEEEEEECCCC	19.80	28857561
231	Phosphorylation	GPRSISVSALPASDS CCCEEEEEECCCCCC	2.57	27080861
236	Phosphorylation	SVSALPASDSPTPSF EEEECCCCCCCCCCC	28.33	27174698
238	Phosphorylation	SALPASDSPTPSFSE EECCCCCCCCCCCCC	35.20	25159151
240	Phosphorylation	LPASDSPTPSFSEGL CCCCCCCCCCCCCCC	43.83	28387310
242	Phosphorylation	ASDSPTPSFSEGLSD CCCCCCCCCCCCCCC	34.78	27174698
244	Phosphorylation	DSPTPSFSEGLSDTC CCCCCCCCCCCCCCC	29.32	21712546
248	Phosphorylation	PSFSEGLSDTCIPLH CCCCCCCCCCCEEEC	14.68	33259812
250	Phosphorylation	FSEGLSDTCIPLHAS CCCCCCCCCEEECCC	4.40	29978859
257	Phosphorylation	TCIPLHASGRLTPRA CCEEECCCCCCCHHH	32.66	29978859
261	Phosphorylation	LHASGRLTPRALHSF ECCCCCCCHHHHHHH	44.54	29978859
267	Phosphorylation	LTPRALHSFITPPTT CCHHHHHHHCCCCCC	6.74	22167270
268	Phosphorylation	TPRALHSFITPPTTP CHHHHHHHCCCCCCH	22.62	18669648
269	Phosphorylation	PRALHSFITPPTTPQ HHHHHHHCCCCCCHH	21.30	19664994
270	Phosphorylation	RALHSFITPPTTPQL HHHHHHCCCCCCHHH	40.01	27273156
272	Phosphorylation	LHSFITPPTTPQLRR HHHHCCCCCCHHHHH	18.14	18669648
273	Phosphorylation	HSFITPPTTPQLRRH HHHCCCCCCHHHHHC	33.42	22167270
274	Phosphorylation	SFITPPTTPQLRRHT HHCCCCCCHHHHHCC	45.57	27273156
288	Phosphorylation	TKLKPPRTPPPPSRK CCCCCCCCCCCCCHH	53.47	27794612
293	Phosphorylation	PRTPPPPSRKVFQLL CCCCCCCCHHHHHHC	52.30	21406692
309	Phosphorylation	SFPTLTRSKSHESQL CCCCCCCCCCCHHHH	33.46	20164059
311	Phosphorylation	PTLTRSKSHESQLGN CCCCCCCCCHHHHCC	47.47	28355574
314	Phosphorylation	TRSKSHESQLGNRID CCCCCCHHHHCCCCH	10.26	22115753
320	Phosphorylation	ESQLGNRIDDVSSMR HHHHCCCCHHHHHCE	39.57	9858547
324	Phosphorylation	GNRIDDVSSMRFDLS CCCCHHHHHCEEECC	3.74	23186163
325	Phosphorylation	NRIDDVSSMRFDLSH CCCHHHHHCEEECCC	24.71	23186163
331	Phosphorylation	SSMRFDLSHGSPQMV HHCEEECCCCCCHHH	37.33	25159151
332	Phosphorylation	SMRFDLSHGSPQMVR HCEEECCCCCCHHHH	13.41	18669648
334	Phosphorylation	RFDLSHGSPQMVRRD EEECCCCCCHHHHCC	44.70	22167270
345	Phosphorylation	VRRDIGLSVTHRFST HHCCCCCCEEECCCC	12.17	23186163
347	Phosphorylation	RDIGLSVTHRFSTKS CCCCCCEEECCCCHH	20.18	23186163
351	Phosphorylation	LSVTHRFSTKSWLSQ CCEEECCCCHHHHHH	38.14	30108239
352	Phosphorylation	SVTHRFSTKSWLSQV CEEECCCCHHHHHHH	33.08	23186163
394	Phosphorylation	EAPACRISFLPLTRL CCCCCEEEEECCCCC	2.44	28555341
399	Phosphorylation	RISFLPLTRLRRTES EEEEECCCCCCCCCC	3.51	28555341
402	Phosphorylation	FLPLTRLRRTESVPS EECCCCCCCCCCCCC	26.61	18220336
404	Phosphorylation	PLTRLRRTESVPSDI CCCCCCCCCCCCCCC	38.42	23401153
406	Phosphorylation	TRLRRTESVPSDINN CCCCCCCCCCCCCCC	35.25	19664994
409	Phosphorylation	RRTESVPSDINNPVD CCCCCCCCCCCCCCH	6.69	30266825
425	Phosphorylation	AAEPHFGTLPKALTK HCCCCCCCCCHHHHC	21.51	30266825
432	Phosphorylation	TLPKALTKKEHPPAM CCCHHHHCCCCCCHH	64.28	32142685
457	Phosphorylation	STTSSTPSSPAPFPT CCCCCCCCCCCCCCC	48.57	9858547
463	Phosphorylation	PSSPAPFPTSSNPSS CCCCCCCCCCCCCCC	28.49	24719451
473	Phosphorylation	SNPSSATTPPNPSPG CCCCCCCCCCCCCCC	64.37	-
478	Phosphorylation	ATTPPNPSPGQRDSR CCCCCCCCCCCCCCC	39.22	-
547	Phosphorylation	ADGTRLDDQPKADVL CCCCCCCCCCCHHHH	40.57	32142685
565	Ubiquitination	EAEAEEPEAGKSEAE HHHHCCCCCCCCCCC	32.15	29967540
567	Phosphorylation	EAEEPEAGKSEAEDD HHCCCCCCCCCCCCC	40.91	18669648
569	Phosphorylation	EEPEAGKSEAEDDED CCCCCCCCCCCCCCC	32.39	28355574
583	Phosphorylation	DEVDDLPSSRRPWRG CCCCCCCCCCCCCCC	53.68	23403867
584	Phosphorylation	EVDDLPSSRRPWRGP CCCCCCCCCCCCCCC	36.76	23403867
597	Phosphorylation	GPISRKASQTSVYLQ CCCCCCCCCCEEEEE	20.05	28857561
599	Phosphorylation	ISRKASQTSVYLQEW CCCCCCCCEEEEEEC	6.67	28857561
600	Phosphorylation	SRKASQTSVYLQEWD CCCCCCCEEEEEECC	11.48	28857561
602	Phosphorylation	KASQTSVYLQEWDIP CCCCCEEEEEECCCC	31.86	28857561
656	Acetylation	DHLKLFKKEVMNYRQ HHHHHHHHHHHCHHH	4.07	7675409
693	Phosphorylation	TSFCKGRTLHSFVRD HHHCCCCCHHHHHCC	20.69	19369195
709	Acetylation	KTSLDINKTRQIAQE CCCCCHHHHHHHHHH	27.76	18603801
723	Phosphorylation	EIIKGMGYLHAKGIV HHHHHCCHHHHCCEE	19.53	-
741	Phosphorylation	LKSKNVFYDNGKVVI CCCCCEEEECCEEEE	40.58	-
751	Phosphorylation	GKVVITDFGLFGISG CEEEEEECEECCHHH	3.43	24719451
844	Phosphorylation	EGMKRVLTSVSLGKE CHHHHHHHHHHCCHH	4.63	23186163
845	Phosphorylation	GMKRVLTSVSLGKEV HHHHHHHHHHCCHHH	31.04	23186163
869	Phosphorylation	FDLQERPSFSLLMDM CCCCCCCCHHHHHHH	25.57	27251275
871	Phosphorylation	LQERPSFSLLMDMLE CCCCCCHHHHHHHHH	2.37	27251275
888	Phosphorylation	PKLNRRLSHPGHFWK HHHHHHHCCCCCCCC	34.28	28152594
918	Phosphorylation	FGLGVLESSNPKM-- CCCCHHCCCCCCC--	49.87	28555341

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
406	S	Phosphorylation	Kinase	MAP3K7	O43318	GPS
888	S	Phosphorylation	Kinase	PRKACA	P17612	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
311	S	Phosphorylation	17081983
Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3.
406	S	Phosphorylation	18669648
Dephosphorylated on Ser-406 by PPP2CA.
406	S	Phosphorylation	18669648
Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3.
888	S	Phosphorylation	-
Phosphorylated by PKA at Ser-888.
888	S	Phosphorylation	-
Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3 (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of KSR1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MP2K1_HUMAN	MAP2K1	physical	18332145
BRAF_HUMAN	BRAF	physical	18332145
KSR1_HUMAN	KSR1	physical	18332145
MARK3_HUMAN	MARK3	physical	12941695
MARK3_HUMAN	MARK3	physical	11741534
RAF1_HUMAN	RAF1	physical	11741534
LSP1_HUMAN	LSP1	physical	15090600
PJA2_HUMAN	PJA2	physical	27195677
KCTD3_HUMAN	KCTD3	physical	27173435
KI13B_HUMAN	KIF13B	physical	27173435
ZBT21_HUMAN	ZBTB21	physical	27173435
GGYF1_HUMAN	GIGYF1	physical	27173435
CING_HUMAN	CGN	physical	27173435
LRFN1_HUMAN	LRFN1	physical	27173435
DEN1A_HUMAN	DENND1A	physical	27173435
SPD2A_HUMAN	SH3PXD2A	physical	27173435
RTKN_HUMAN	RTKN	physical	27173435
DEN4C_HUMAN	DENND4C	physical	27173435
MAST3_HUMAN	MAST3	physical	27173435
SRGP2_HUMAN	SRGAP2	physical	27173435
IF4E2_HUMAN	EIF4E2	physical	27173435
SI1L1_HUMAN	SIPA1L1	physical	27173435
PPM1H_HUMAN	PPM1H	physical	27173435
LPIN3_HUMAN	LPIN3	physical	27173435
LIMA1_HUMAN	LIMA1	physical	27173435
MAGI1_HUMAN	MAGI1	physical	27173435
TESK2_HUMAN	TESK2	physical	27173435
DCLK1_HUMAN	DCLK1	physical	27173435
ZN638_HUMAN	ZNF638	physical	27173435
SIN1_HUMAN	MAPKAP1	physical	27173435
NF1_HUMAN	NF1	physical	27173435
HDAC4_HUMAN	HDAC4	physical	27173435
CBY1_HUMAN	CBY1	physical	27173435
SH3R3_HUMAN	SH3RF3	physical	27173435
SRS12_HUMAN	SRSF12	physical	27173435
MPIP3_HUMAN	CDC25C	physical	27173435
SYDE1_HUMAN	SYDE1	physical	27173435
AGAP1_HUMAN	AGAP1	physical	27173435
NADK_HUMAN	NADK	physical	27173435
GGYF2_HUMAN	GIGYF2	physical	27173435
MPIP2_HUMAN	CDC25B	physical	27173435
CDK16_HUMAN	CDK16	physical	27173435
TANC2_HUMAN	TANC2	physical	27173435
CAMP2_HUMAN	CAMSAP2	physical	27173435
PTN13_HUMAN	PTPN13	physical	27173435
RGPS2_HUMAN	RALGPS2	physical	27173435
F110B_HUMAN	FAM110B	physical	27173435
UBP21_HUMAN	USP21	physical	27173435
M3K21_HUMAN	KIAA1804	physical	27173435
NGAP_HUMAN	RASAL2	physical	27173435
TIAM1_HUMAN	TIAM1	physical	27173435
AN34A_HUMAN	ANKRD34A	physical	27173435
FA53C_HUMAN	FAM53C	physical	27173435
PHLB2_HUMAN	PHLDB2	physical	27173435
RPTOR_HUMAN	RPTOR	physical	27173435
PKHA7_HUMAN	PLEKHA7	physical	27173435
OSBL6_HUMAN	OSBPL6	physical	27173435
KIF1C_HUMAN	KIF1C	physical	27173435
MELK_HUMAN	MELK	physical	27173435
NAV1_HUMAN	NAV1	physical	27173435
F110A_HUMAN	FAM110A	physical	27173435
GAB2_HUMAN	GAB2	physical	27173435
STA13_HUMAN	STARD13	physical	27173435
PTN14_HUMAN	PTPN14	physical	27173435
DEP1B_HUMAN	DEPDC1B	physical	27173435
HDAC7_HUMAN	HDAC7	physical	27173435
CWC25_HUMAN	CWC25	physical	27173435
INP5E_HUMAN	INPP5E	physical	27173435
LARP1_HUMAN	LARP1	physical	27173435
RFIP2_HUMAN	RAB11FIP2	physical	27173435
PKHA5_HUMAN	PLEKHA5	physical	27173435
MFR1L_HUMAN	MTFR1L	physical	27173435
AFAD_HUMAN	MLLT4	physical	27173435
CHK1_HUMAN	CHEK1	physical	27173435
KIF1B_HUMAN	KIF1B	physical	27173435
KC1A_HUMAN	CSNK1A1	physical	27173435
TBC25_HUMAN	TBC1D25	physical	27173435
HDAC5_HUMAN	HDAC5	physical	27173435
TBCD1_HUMAN	TBC1D1	physical	27173435
DACT3_HUMAN	DACT3	physical	27173435
PANK2_HUMAN	PANK2	physical	27173435
REEP3_HUMAN	REEP3	physical	27173435
CRTC2_HUMAN	CRTC2	physical	27173435
CRTC3_HUMAN	CRTC3	physical	27173435
MILK1_HUMAN	MICALL1	physical	27173435
SH3B4_HUMAN	SH3BP4	physical	27173435
BAIP2_HUMAN	BAIAP2	physical	27173435
FGD6_HUMAN	FGD6	physical	27173435

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of KSR1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-404, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-309 AND SER-404, ANDMASS SPECTROMETRY.	19369195 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-268; THR-272; SER-332;SER-404 AND SER-567, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-268; THR-272 ANDSER-332, AND MASS SPECTROMETRY.	18220336 [Abstract]
"Phosphoproteome of resting human platelets."; Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J.,Schuetz C., Walter U., Gambaryan S., Sickmann A.; J. Proteome Res. 7:526-534(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-268 AND SER-309, ANDMASS SPECTROMETRY.	18088087 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-309, AND MASSSPECTROMETRY.	17081983 [Abstract]

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