dbPTM

PJA2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PJA2_HUMAN
UniProt AC	O43164
Protein Name	E3 ubiquitin-protein ligase Praja-2
Gene Name	PJA2
Organism	Homo sapiens (Human).
Sequence Length	708
Subcellular Localization	Cytoplasm . Cell membrane . Endoplasmic reticulum membrane Peripheral membrane protein . Golgi apparatus membrane Peripheral membrane protein . Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Loca
Protein Description	Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes..
Protein Sequence	MSQYTEKEPAAMDQESGKAVWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENSSGSSPLDQVDSSLPSEPIFEKSETEIPTCGSALNQTTESSQSFVAVHHSEEGRDTLGSSTNLHNHSEGEYIPGACSASSVQNGIALVHTDSYDPDGKHGEDNDHLQLSAEVVEGSRYQESLGNTVFELENREAEAYTGLSPPVPSFNCEVRDEFEELDSVPLVKSSAGDTEFVHQNSQEIQRSSQDEMVSTKQQNNTSQERQTEHSPEDAACGPGHICSEQNTNDREKNHGSSPEQVVRPKVRKLISSSQVDQETGFNRHEAKQRSVQRWREALEVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENNQMTSESGATAGRQEVDNTFWNGCGDYYQLYDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIEASAAPSSEPDPDAPPSNDSIAEAP

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSQYTEKEP ------CCCCCCCCC	31.71	-
2	Phosphorylation	------MSQYTEKEP ------CCCCCCCCC	31.71	23186163
4	Phosphorylation	----MSQYTEKEPAA ----CCCCCCCCCCC	15.01	20068231
5	Phosphorylation	---MSQYTEKEPAAM ---CCCCCCCCCCCC	32.70	23186163
16	Phosphorylation	PAAMDQESGKAVWPK CCCCCCCCCCEECCC	39.70	28985074
18	Ubiquitination	AMDQESGKAVWPKPA CCCCCCCCEECCCCC	49.39	29967540
28	Phosphorylation	WPKPAGGYQTITGRR CCCCCCCEEEECCCE	11.10	28796482
30	Phosphorylation	KPAGGYQTITGRRYG CCCCCEEEECCCEEC	16.42	-
42	Phosphorylation	RYGRRHAYVSFKPCM EECCCEEEEEECCCC	7.18	28796482
44	Phosphorylation	GRRHAYVSFKPCMTR CCCEEEEEECCCCCH	18.18	30576142
46	Acetylation	RHAYVSFKPCMTRHE CEEEEEECCCCCHHH	29.78	23749302
46	Methylation	RHAYVSFKPCMTRHE CEEEEEECCCCCHHH	29.78	66725095
50	Phosphorylation	VSFKPCMTRHERSLG EEECCCCCHHHHHHC	35.27	26434776
63	Phosphorylation	LGRAGDDYEVLELDD HCCCCCCEEEEEHHC	16.81	28796482
76	Phosphorylation	DDVPKENSSGSSPLD HCCCCCCCCCCCCHH	36.85	22199227
77	Phosphorylation	DVPKENSSGSSPLDQ CCCCCCCCCCCCHHH	55.03	22199227
79	Phosphorylation	PKENSSGSSPLDQVD CCCCCCCCCCHHHCC	30.63	22199227
80	Phosphorylation	KENSSGSSPLDQVDS CCCCCCCCCHHHCCC	32.76	26657352
87	Phosphorylation	SPLDQVDSSLPSEPI CCHHHCCCCCCCCCC	34.61	22199227
88	Phosphorylation	PLDQVDSSLPSEPIF CHHHCCCCCCCCCCC	39.57	22199227
91	Phosphorylation	QVDSSLPSEPIFEKS HCCCCCCCCCCCCCC	62.39	26074081
134	Phosphorylation	EGRDTLGSSTNLHNH CCCCCCCCCCCCCCC	36.78	24275569
152	Phosphorylation	EYIPGACSASSVQNG CCCCCCCCCCHHCCC	30.56	28348404
154	Phosphorylation	IPGACSASSVQNGIA CCCCCCCCHHCCCEE	18.10	28348404
155	Phosphorylation	PGACSASSVQNGIAL CCCCCCCHHCCCEEE	27.69	28348404
165	Phosphorylation	NGIALVHTDSYDPDG CCEEEEECCCCCCCC	21.11	28348404
167	Phosphorylation	IALVHTDSYDPDGKH EEEEECCCCCCCCCC	32.37	28348404
168	Phosphorylation	ALVHTDSYDPDGKHG EEEECCCCCCCCCCC	34.01	28348404
193	Phosphorylation	EVVEGSRYQESLGNT EHHHCCHHHHHHCCE	20.52	20873877
196	Phosphorylation	EGSRYQESLGNTVFE HCCHHHHHHCCEEEE	26.50	23663014
200	Phosphorylation	YQESLGNTVFELENR HHHHHCCEEEEEECC	25.44	20873877
212	Phosphorylation	ENREAEAYTGLSPPV ECCCHHHHCCCCCCC	7.89	28796482
213	Phosphorylation	NREAEAYTGLSPPVP CCCHHHHCCCCCCCC	38.40	28796482
216	Phosphorylation	AEAYTGLSPPVPSFN HHHHCCCCCCCCCCC	28.62	25159151
221	Phosphorylation	GLSPPVPSFNCEVRD CCCCCCCCCCEEECH	29.35	28387310
235	Phosphorylation	DEFEELDSVPLVKSS HHHHHHCCCCEEECC	37.95	19664994
241	Phosphorylation	DSVPLVKSSAGDTEF CCCCEEECCCCCCCC	20.24	29691806
242	Phosphorylation	SVPLVKSSAGDTEFV CCCEEECCCCCCCCC	30.47	29691806
246	Phosphorylation	VKSSAGDTEFVHQNS EECCCCCCCCCCCCH	30.33	29691806
253	Phosphorylation	TEFVHQNSQEIQRSS CCCCCCCHHHHHHHC	23.71	23401153
259	Phosphorylation	NSQEIQRSSQDEMVS CHHHHHHHCHHHHHH	18.90	21406692
260	Phosphorylation	SQEIQRSSQDEMVST HHHHHHHCHHHHHHH	43.79	21406692
266	Phosphorylation	SSQDEMVSTKQQNNT HCHHHHHHHHHHCCC	28.69	21406692
267	Phosphorylation	SQDEMVSTKQQNNTS CHHHHHHHHHHCCCH	22.82	21406692
268 (in isoform 1)	Ubiquitination	-	42.24	21890473
268	Ubiquitination	QDEMVSTKQQNNTSQ HHHHHHHHHHCCCHH	42.24	27667366
268 (in isoform 2)	Ubiquitination	-	42.24	21890473
273	Phosphorylation	STKQQNNTSQERQTE HHHHHCCCHHHHHHC	39.06	29759185
274	Phosphorylation	TKQQNNTSQERQTEH HHHHCCCHHHHHHCC	31.97	21406692
282	Phosphorylation	QERQTEHSPEDAACG HHHHHCCCCCHHCCC	24.61	25159151
304	Ubiquitination	QNTNDREKNHGSSPE CCCCHHHHCCCCCHH	55.95	29967540
308	Phosphorylation	DREKNHGSSPEQVVR HHHHCCCCCHHHHHC	34.86	30266825
309	Phosphorylation	REKNHGSSPEQVVRP HHHCCCCCHHHHHCH	36.73	25159151
317	Ubiquitination	PEQVVRPKVRKLISS HHHHHCHHHHHHHCC	44.07	23000965
320	Ubiquitination	VVRPKVRKLISSSQV HHCHHHHHHHCCCCC	53.83	23000965
320 (in isoform 1)	Ubiquitination	-	53.83	21890473
320 (in isoform 2)	Ubiquitination	-	53.83	21890473
323	Phosphorylation	PKVRKLISSSQVDQE HHHHHHHCCCCCCHH	34.08	30266825
324	Phosphorylation	KVRKLISSSQVDQET HHHHHHCCCCCCHHH	20.14	30266825
325	Phosphorylation	VRKLISSSQVDQETG HHHHHCCCCCCHHHC	27.68	19690332
339	Ubiquitination	GFNRHEAKQRSVQRW CCCHHHHHHHHHHHH	43.17	21890473
339 (in isoform 2)	Ubiquitination	-	43.17	21890473
339 (in isoform 1)	Ubiquitination	-	43.17	21890473
342	Phosphorylation	RHEAKQRSVQRWREA HHHHHHHHHHHHHHH	21.80	21423175
355	Phosphorylation	EALEVEESGSDDLLI HHHHHHHHCCCCEEE	30.05	20873877
357	Phosphorylation	LEVEESGSDDLLIKC HHHHHHCCCCEEEEE	36.91	20873877
367	Phosphorylation	LLIKCEEYDGEHDCM EEEEEEEECCCCCEE	13.62	-
380	Phosphorylation	CMFLDPPYSRVITQR EEEECCCCCEEEEEH	17.94	22817900
381	Phosphorylation	MFLDPPYSRVITQRE EEECCCCCEEEEEHH	26.33	22817900
385	Phosphorylation	PPYSRVITQRETENN CCCCEEEEEHHCCCC	20.79	22817900
389	Phosphorylation	RVITQRETENNQMTS EEEEEHHCCCCCCCC	46.32	20873877
395	Phosphorylation	ETENNQMTSESGATA HCCCCCCCCCCCCCC	21.41	20873877
396	Phosphorylation	TENNQMTSESGATAG CCCCCCCCCCCCCCC	25.15	20873877
398	Phosphorylation	NNQMTSESGATAGRQ CCCCCCCCCCCCCCH	33.23	20873877
428	Phosphorylation	LYDKDEDSSECSDGE EEECCCCCCCCCCCC	26.45	25159151
429	Phosphorylation	YDKDEDSSECSDGEW EECCCCCCCCCCCCE	55.75	25159151
432	Phosphorylation	DEDSSECSDGEWSAS CCCCCCCCCCCEECC	44.60	25159151
437	Phosphorylation	ECSDGEWSASLPHRF CCCCCCEECCCCCCC	12.04	27251275
452	Phosphorylation	SGTEKDQSSSDESWE CCCCCCCCCCCCCCC	41.78	20363803
453	Phosphorylation	GTEKDQSSSDESWET CCCCCCCCCCCCCCC	35.85	20363803
454	Phosphorylation	TEKDQSSSDESWETL CCCCCCCCCCCCCCC	51.12	20363803
457	Phosphorylation	DQSSSDESWETLPGK CCCCCCCCCCCCCCC	34.89	20363803
460	Phosphorylation	SSDESWETLPGKDEN CCCCCCCCCCCCCCC	31.81	25159151
486	Phosphorylation	EEENQELSLQEGEQT HHHHHCCCCCCCCCC	27.53	26074081
493	Phosphorylation	SLQEGEQTSLEEGEI CCCCCCCCCCCCCCC	30.86	26074081
494	Phosphorylation	LQEGEQTSLEEGEIP CCCCCCCCCCCCCCC	33.21	26074081
576	Phosphorylation	VDPQFLTYMALEERL CCHHHHHHHHHHHHH	5.20	22817900
596	Phosphorylation	TALAHLESLAVDVEV HHHHHHHHHCCEEEE	28.17	24275569
647 (in isoform 2)	Ubiquitination	-	11.30	21890473
658	Ubiquitination	PCHHFFHKPCVSIWL CCCHHCCCCEEEEEE	34.65	29967540
667	Ubiquitination	CVSIWLQKSGTCPVC EEEEEEECCCCCCCC	49.00	22817900
667 (in isoform 1)	Ubiquitination	-	49.00	21890473

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
342	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
342	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
342	S	Phosphorylation	Kinase	PKA	-	Uniprot
389	T	Phosphorylation	Kinase	PRKACA	P17612	GPS
389	T	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
389	T	Phosphorylation	Kinase	PKA	-	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	PJA2	O43164	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of PJA2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PJA2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UB2D2_HUMAN	UBE2D2	physical	12036302
KAP2_HUMAN	PRKAR2A	physical	21423175
KAP0_HUMAN	PRKAR1A	physical	21423175
UB2D3_HUMAN	UBE2D3	physical	21423175
PJA2_HUMAN	PJA2	physical	12036302
KSR1_HUMAN	KSR1	physical	27195677
MFHA1_HUMAN	MFHAS1	physical	28471450

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PJA2_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Control of PKA stability and signalling by the RING ligase praja2."; Lignitto L., Carlucci A., Sepe M., Stefan E., Cuomo O., Nistico R.,Scorziello A., Savoia C., Garbi C., Annunziato L., Feliciello A.; Nat. Cell Biol. 13:412-422(2011). Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PRKAR1A; PRKAR2A ANDPRKAR2B, AND PHOSPHORYLATION AT SER-342 AND THR-389.	21423175 [Abstract]
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-380; SER-381; THR-385;SER-428; SER-429 AND SER-432, AND MASS SPECTROMETRY.	17081983 [Abstract]