dbPTM

BRAF_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	BRAF_MOUSE
UniProt AC	P28028
Protein Name	Serine/threonine-protein kinase B-raf
Gene Name	Braf
Organism	Mus musculus (Mouse).
Sequence Length	804
Subcellular Localization	Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes..
Protein Description	Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron (By similarity)..
Protein Sequence	MAALSGGGGSSSGGGGGGGGGGGGGDGGGGAEQGQALFNGDMEPEAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLVFQTPTDASRNNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPVPQEEASFPETALPSGSSSAPPSDSTGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDEKFPEVELQDQRDLIRDQGFRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSSSEDRSRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAALSGGGG ------CCCCCCCCC	19.67	-
9 (in isoform 2)	Phosphorylation	-	27.85	25266776
10 (in isoform 2)	Phosphorylation	-	25.30	25266776
134	Phosphorylation	DASRNNPKSPQKPIV HHHCCCCCCCCCCEE	76.52	26824392
135	Phosphorylation	ASRNNPKSPQKPIVR HHCCCCCCCCCCEEE	33.54	26824392
147	Ubiquitination	IVRVFLPNKQRTVVP EEEEECCCCCCCEEE	56.06	-
148	Ubiquitination	VRVFLPNKQRTVVPA EEEECCCCCCCEEEC	38.94	22790023
298	Phosphorylation	FPETALPSGSSSAPP CCCCCCCCCCCCCCC	52.75	-
300	Phosphorylation	ETALPSGSSSAPPSD CCCCCCCCCCCCCCC	26.00	-
301	Phosphorylation	TALPSGSSSAPPSDS CCCCCCCCCCCCCCC	33.76	-
313 (in isoform 2)	Phosphorylation	-	5.22	26745281
315	Phosphorylation	STGPQILTSPSPSKS CCCCCCCCCCCCCCC	39.97	29899451
316 (in isoform 2)	Phosphorylation	-	21.67	25266776
316	Phosphorylation	TGPQILTSPSPSKSI CCCCCCCCCCCCCCC	21.67	22817900
318	Phosphorylation	PQILTSPSPSKSIPI CCCCCCCCCCCCCCC	41.39	19060867
320	Phosphorylation	ILTSPSPSKSIPIPQ CCCCCCCCCCCCCCC	43.29	21082442
323 (in isoform 2)	Phosphorylation	-	4.05	26745281
345	Phosphorylation	NQFGQRDRSSSAPNV HCCCCCCCCCCCCCE	40.40	25521595
346	Phosphorylation	QFGQRDRSSSAPNVH CCCCCCCCCCCCCEE	32.69	25521595
346	Phosphorylation	QFGQRDRSSSAPNVH CCCCCCCCCCCCCEE	32.69	22802335
347	Phosphorylation	FGQRDRSSSAPNVHI CCCCCCCCCCCCEEE	31.26	25521595
347	Phosphorylation	FGQRDRSSSAPNVHI CCCCCCCCCCCCEEE	31.26	25521595
348	Phosphorylation	GQRDRSSSAPNVHIN CCCCCCCCCCCEEEC	49.33	25521595
355	Phosphorylation	SAPNVHINTIEPVNI CCCCEEECEEECCCC	21.81	25168779
356	Phosphorylation	APNVHINTIEPVNID CCCEEECEEECCCCC	26.56	25521595
376	Phosphorylation	VELQDQRDLIRDQGF CEECCHHHHHHHCCC	40.59	26643407
377	Phosphorylation	ELQDQRDLIRDQGFR EECCHHHHHHHCCCC	3.64	26160508
378	Phosphorylation	LQDQRDLIRDQGFRG ECCHHHHHHHCCCCC	5.52	27180971
379	Phosphorylation	QDQRDLIRDQGFRGD CCHHHHHHHCCCCCC	37.18	-
381	Phosphorylation	QRDLIRDQGFRGDGA HHHHHHHCCCCCCCC	42.97	26745281
382	Phosphorylation	RDLIRDQGFRGDGAP HHHHHHCCCCCCCCH	20.64	-
383	Phosphorylation	DLIRDQGFRGDGAPL HHHHHCCCCCCCCHH	7.02	25521595
384	Phosphorylation	LIRDQGFRGDGAPLN HHHHCCCCCCCCHHH	49.47	-
387	Phosphorylation	DQGFRGDGAPLNQLM HCCCCCCCCHHHHHH	31.73	26745281
391	Phosphorylation	RGDGAPLNQLMRCLR CCCCCHHHHHHHHHH	31.70	26643407
393	Phosphorylation	DGAPLNQLMRCLRKY CCCHHHHHHHHHHHH	1.97	25777480
431	Phosphorylation	PVFRGSTTGLSATPP CCCCCCCCCCCCCCC	37.51	17242355
432	Phosphorylation	VFRGSTTGLSATPPA CCCCCCCCCCCCCCC	20.09	21183079
434	Phosphorylation	RGSTTGLSATPPASL CCCCCCCCCCCCCCC	31.29	-
436	Phosphorylation	STTGLSATPPASLPG CCCCCCCCCCCCCCC	26.82	29899451
444	Phosphorylation	PPASLPGSLTNVKAL CCCCCCCCCCCHHHH	30.04	-
454	Phosphorylation	NVKALQKSPGPQRER CHHHHHCCCCCCCCC	23.33	26824392
463	Phosphorylation	GPQRERKSSSSSSSE CCCCCCCCCCCCCHH	41.49	23375375
464	Phosphorylation	PQRERKSSSSSSSED CCCCCCCCCCCCHHH	36.96	27087446
465	Phosphorylation	QRERKSSSSSSSEDR CCCCCCCCCCCHHHH	41.13	-
466	Phosphorylation	RERKSSSSSSSEDRS CCCCCCCCCCHHHHH	35.87	-
467	Phosphorylation	ERKSSSSSSSEDRSR CCCCCCCCCHHHHHH	39.57	-
477	Phosphorylation	EDRSRMKTLGRRDSS HHHHHHHHCCCCCCC	25.71	27087446
483	Phosphorylation	KTLGRRDSSDDWEIP HHCCCCCCCCCCCCC	33.58	18388127
484	Phosphorylation	TLGRRDSSDDWEIPD HCCCCCCCCCCCCCC	43.83	25521595
495	Phosphorylation	EIPDGQITVGQRIGS CCCCCEEEEEEEEEC	15.65	25619855
502	Phosphorylation	TVGQRIGSGSFGTVY EEEEEEECCCCCEEE	28.97	26745281
504	Phosphorylation	GQRIGSGSFGTVYKG EEEEECCCCCEEECC	23.38	28066266
507	Phosphorylation	IGSGSFGTVYKGKWH EECCCCCEEECCEEE	20.74	28066266
636	Phosphorylation	IGDFGLATVKSRWSG ECCCEEEEEHHCCCC	33.24	-
639	Phosphorylation	FGLATVKSRWSGSHQ CEEEEEHHCCCCCCC	34.35	23984901
642	Phosphorylation	ATVKSRWSGSHQFEQ EEEHHCCCCCCCHHH	29.34	26643407
644	Phosphorylation	VKSRWSGSHQFEQLS EHHCCCCCCCHHHHC	14.48	26643407
651	Phosphorylation	SHQFEQLSGSILWMA CCCHHHHCCCEEEEC	30.13	23984901
653	Phosphorylation	QFEQLSGSILWMAPE CHHHHCCCEEEECHH	15.90	23984901
656	Methylation	QLSGSILWMAPEVIR HHCCCEEEECHHHHC	4.53	-
708	Methylation	QIIFMVGRGYLSPDL EEEEEECCCCCCCCH	21.12	-
712	Phosphorylation	MVGRGYLSPDLSKVR EECCCCCCCCHHHHH	14.10	-
714	Phosphorylation	GRGYLSPDLSKVRSN CCCCCCCCHHHHHHC	61.26	-
734	Phosphorylation	KRLMAECLKKKRDER HHHHHHHHHHCCCCC	7.39	26745281
735	Phosphorylation	RLMAECLKKKRDERP HHHHHHHHHCCCCCC	68.83	21183079
737	Phosphorylation	MAECLKKKRDERPLF HHHHHHHCCCCCCCH	64.59	26745281
738	Phosphorylation	AECLKKKRDERPLFP HHHHHHCCCCCCCHH	60.96	-
744	Phosphorylation	KRDERPLFPQILASI CCCCCCCHHHHHHHH	4.66	26745281
764	Phosphorylation	SLPKIHRSASEPSLN CCHHHHHCCCCCCCC	23.22	27087446
766	Phosphorylation	PKIHRSASEPSLNRA HHHHHCCCCCCCCCC	51.81	18388127
769	Phosphorylation	HRSASEPSLNRAGFQ HHCCCCCCCCCCCCC	33.94	23335269
777	Phosphorylation	LNRAGFQTEDFSLYA CCCCCCCCCCCEEEE	34.95	26643407
781	Phosphorylation	GFQTEDFSLYACASP CCCCCCCEEEEECCC	32.56	26745281
783	Phosphorylation	QTEDFSLYACASPKT CCCCCEEEEECCCCC	9.84	22324799
787	Phosphorylation	FSLYACASPKTPIQA CEEEEECCCCCCCCC	27.15	25521595
790	Phosphorylation	YACASPKTPIQAGGY EEECCCCCCCCCCCC	29.07	25521595
797	Phosphorylation	TPIQAGGYGEFAAFK CCCCCCCCCCCCCCC	16.74	29899451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
766	S	Phosphorylation	Kinase	PRKAA1	Q13131	GPS
790	T	Phosphorylation	Kinase	MAPK1	P63085	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
348	S	Phosphorylation	21183079
Phosphorylation at Ser-348 by SGK1 inhibits its activity.
615	K	ubiquitylation	-
Polyubiquitinated at Lys-615 in response to EGF (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of BRAF_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RAF1_MOUSE	Raf1	physical	18332145
AKAP8_MOUSE	Akap8	physical	27034005
ENPL_MOUSE	Hsp90b1	physical	27034005
CSK2B_MOUSE	Csnk2b	physical	27034005
HSP7C_MOUSE	Hspa8	physical	27034005
PHB2_MOUSE	Phb2	physical	27034005
ANXA2_MOUSE	Anxa2	physical	27034005
G3BP2_MOUSE	G3bp2	physical	27034005
CDK1_MOUSE	Cdk1	physical	27034005
ANXA5_MOUSE	Anxa5	physical	27034005
GRP75_MOUSE	Hspa9	physical	27034005
SNX9_MOUSE	Snx9	physical	27034005
PHB_MOUSE	Phb	physical	27034005
VIME_MOUSE	Vim	physical	27034005
1433S_MOUSE	Sfn	physical	27034005
PGAM1_MOUSE	Pgam1	physical	27034005
DNJA1_MOUSE	Dnaja1	physical	27034005
IQGA1_MOUSE	Iqgap1	physical	27034005
1433T_MOUSE	Ywhaq	physical	27034005
1433B_MOUSE	Ywhab	physical	27034005
1433E_MOUSE	Ywhae	physical	27034005
1433Z_MOUSE	Ywhaz	physical	27034005
GNAI2_MOUSE	Gnai2	physical	27034005
1433G_MOUSE	Ywhag	physical	27034005
CDC37_MOUSE	Cdc37	physical	27034005
HS90A_MOUSE	Hsp90aa1	physical	27034005
NPM_MOUSE	Npm1	physical	27034005
WDR26_MOUSE	Wdr26	physical	27034005
MCM3_MOUSE	Mcm3	physical	27034005
G6PI_MOUSE	Gpi1	physical	27034005
ECM29_MOUSE	AI314180	physical	27034005
GNAI3_MOUSE	Gnai3	physical	27034005
CDK2_MOUSE	Cdk2	physical	27034005
DJB12_MOUSE	Dnajb12	physical	27034005
PP1RA_MOUSE	Ppp1r10	physical	27034005
FMNL3_MOUSE	Fmnl3	physical	27034005
MSHR_MOUSE	Mc1r	physical	27034005
SGPL1_MOUSE	Sgpl1	physical	27034005
DJB11_MOUSE	Dnajb11	physical	27034005
RAP1B_MOUSE	Rap1b	physical	27034005
MYOF_MOUSE	Myof	physical	27034005
RASH_MOUSE	Hras	physical	27034005
COPA_MOUSE	Copa	physical	27034005
PP1A_MOUSE	Ppp1ca	physical	27034005
PI42C_MOUSE	Pip4k2c	physical	27034005
1433F_MOUSE	Ywhah	physical	27034005
MP2K2_MOUSE	Map2k2	physical	27034005
MP2K1_MOUSE	Map2k1	physical	27034005
GNAS2_MOUSE	Gnas	physical	27034005
ALEX_MOUSE	Gnas	physical	27034005
GNAS1_MOUSE	Gnas	physical	27034005
GNAS3_MOUSE	Gnas	physical	27034005
PP2AB_MOUSE	Ppp2cb	physical	27034005
GRP78_MOUSE	Hspa5	physical	27034005
RAN_MOUSE	Ran	physical	27034005
ATAD3_MOUSE	Atad3a	physical	27034005
UBP7_MOUSE	Usp7	physical	27034005
RAF1_MOUSE	Raf1	physical	27034005

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of BRAF_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-483, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-483, AND MASSSPECTROMETRY.	17242355 [Abstract]

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