GNAI3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GNAI3_MOUSE
• UniProt AC: Q9DC51
• Protein Name: Guanine nucleotide-binding protein G(k) subunit alpha
• Gene Name: Gnai3
• Organism: Mus musculus (Mouse).
• Sequence Length: 354
• Subcellular Localization: Cytoplasm . Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Membrane ; Lipid-anchor . Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
• Protein Description: Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. Stimulates the activity of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division..
• Protein Sequence: MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGESARADDARQLFVLAGSAEEGVMTSELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQTNYIPTQQDVLRTRVKTTGIVETHFTFKELYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEVYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Myristoylation	------MGCTLSAED ------CCCCCCHHH	17.08	-
3	S-palmitoylation	-----MGCTLSAEDK -----CCCCCCHHHH	3.14	-
17	Ubiquitination	KAAVERSKMIDRNLR HHHHHHHHHHCHHHH	46.20	27667366
35	Ubiquitination	EKAAKEVKLLLLGAG HHHHHHHHHHEECCC	34.21	-
44	Phosphorylation	LLLGAGESGKSTIVK HEECCCCCCCCHHHH	51.07	22817900
46	Ubiquitination	LGAGESGKSTIVKQM ECCCCCCCCHHHHEE	54.42	27667366
47	Phosphorylation	GAGESGKSTIVKQMK CCCCCCCCHHHHEEE	27.22	29899451
48	Phosphorylation	AGESGKSTIVKQMKI CCCCCCCHHHHEEEE	33.02	-
51	Ubiquitination	SGKSTIVKQMKIIHE CCCCHHHHEEEEECC	40.60	27667366
62	Phosphorylation	IIHEDGYSEDECKQY EECCCCCCHHHHCEE	44.06	28973931
69	Phosphorylation	SEDECKQYKVVVYSN CHHHHCEEEEEEECC	7.40	-
92	Ubiquitination	IRAMGRLKIDFGESA HHHHCCCCCCCCCCC	38.58	22790023
98	Phosphorylation	LKIDFGESARADDAR CCCCCCCCCCHHHHH	24.35	22817900
139	S-palmitoylation	RDGGVQACFSRSREY HCCCCEEEEECCCCE	1.45	28680068
180	Ubiquitination	DVLRTRVKTTGIVET HHHHHHCCCCCEEEE	36.76	-
192	Ubiquitination	VETHFTFKELYFKMF EEEEEEHHHHHHHEE	43.69	-
206	Phosphorylation	FDVGGQRSERKKWIH ECCCCCCCCCHHHHH	33.59	22817900
248	Ubiquitination	NRMHESMKLFDSICN HHHHHHHHHHHHHHC	56.11	-
252	Phosphorylation	ESMKLFDSICNNKWF HHHHHHHHHHCCCCC	23.00	29899451
271	Ubiquitination	IILFLNKKDLFEEKI EEEEEEHHHHHHHHH	59.60	27667366
277	Ubiquitination	KKDLFEEKIKRSPLT HHHHHHHHHHCCCCE	46.45	-
330	Ubiquitination	FTCATDTKNVQFVFD EEECCCCCCCEEEEE	58.91	-
345	Ubiquitination	AVTDVIIKNNLKECG HHHHHHHHCCHHHCC	28.76	-
349	Ubiquitination	VIIKNNLKECGLY-- HHHHCCHHHCCCC--	53.75	-
354	Phosphorylation	NLKECGLY------- CHHHCCCC-------	11.20	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of GNAI3_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of GNAI3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GNAI3_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of GNAI3_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.