MYOF_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MYOF_MOUSE
• UniProt AC: Q69ZN7
• Protein Name: Myoferlin
• Gene Name: Myof
• Organism: Mus musculus (Mouse).
• Sequence Length: 2048
• Subcellular Localization: Cell membrane; Single-pass type II membrane protein. Nucleus membrane; Single-pass type II membrane protein. Cytoplasmic vesicle membrane; Single-pass type II membrane protein. Found at nuclear and plasma membranes. Enriched in undifferentiated myobl
• Protein Description: Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR..
• Protein Sequence: MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQTSLLNEKGQDTGATIDLVIGYTPPSAPHPNDPSGTSVPGMGEEEEEDQGDEDRVDGIVRGPGPKGPSGTVSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPPEKRDRDNDSDDVESNLLLPAGIALRWVTFMLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEATTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRIFVELNTFLEKKPPEKKLEPISSDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPANQLAEVWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRSRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKTNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNENRYPGGEWKQAEDTYTDANGDKAASPSEMTCPPGWEWEDDAWIYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGEEKGPEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIFGEPQTVLQNPPNVTIELFDNDQVGKDEFLGRSICSPLVKLNSETDITPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCREVVIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIRYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEDMSDIYVKGWISGSEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQRSMKGWWPCYADKDGTRVMAGKVEMTLEVLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFVAVLLYSLPNYLSMKIVRPNA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
16	Acetylation	ATNIPKTKFGKPDPI CCCCCCCCCCCCCCE	59.24	23806337
19	Acetylation	IPKTKFGKPDPIVSV CCCCCCCCCCCEEEE	49.75	23236377
102	Ubiquitination	QNRSLPYKQTSLLNE CCCCCCCCHHHHCCC	45.20	-
170	Phosphorylation	GPGPKGPSGTVSEAQ CCCCCCCCCCCCHHH	56.62	21082442
172	Phosphorylation	GPKGPSGTVSEAQLA CCCCCCCCCCHHHHH	25.79	26824392
174	Phosphorylation	KGPSGTVSEAQLARR CCCCCCCCHHHHHHH	27.35	27087446
183	Phosphorylation	AQLARRITKGKSSRR HHHHHHHHCCHHHHH	31.75	29550500
193	Phosphorylation	KSSRRMLSNKPQDFQ HHHHHHHCCCCCCCE	33.20	27899381
313	Acetylation	EDTSSGAKGYMKVSM CCCCCCCCCCEEEEE	54.84	23236377
315	Phosphorylation	TSSGAKGYMKVSMFV CCCCCCCCEEEEEEE	7.95	29895711
319	Phosphorylation	AKGYMKVSMFVLGTG CCCCEEEEEEEEECC	10.86	29895711
325	Phosphorylation	VSMFVLGTGDEPPPE EEEEEEECCCCCCCC	36.98	28059163
340	Phosphorylation	KRDRDNDSDDVESNL CCCCCCCCCCHHHHC	41.80	27087446
345	Phosphorylation	NDSDDVESNLLLPAG CCCCCHHHHCHHCHH	32.40	25619855
406	Acetylation	VEVSFAGKKVCTNII EEEEECCHHHHCCHH	38.31	30590635
456	Phosphorylation	TKNDVVGTTYLYLSK CCCCCCCHHHEEHHH	10.72	24759943
457	Phosphorylation	KNDVVGTTYLYLSKI CCCCCCHHHEEHHHH	12.95	24759943
458	Phosphorylation	NDVVGTTYLYLSKIA CCCCCHHHEEHHHHH	8.00	24759943
540	Acetylation	EKKPPEKKLEPISSD CCCCCCCCCCCCCCC	57.32	23806337
545	Phosphorylation	EKKLEPISSDDLLVV CCCCCCCCCCCEEEH	38.13	29550500
546	Phosphorylation	KKLEPISSDDLLVVE CCCCCCCCCCEEEHH	35.54	23984901
562	Phosphorylation	YQRRRKYSLSAVFHS HHHHHCCCHHHHHHH	20.91	26239621
564	Phosphorylation	RRRKYSLSAVFHSAT HHHCCCHHHHHHHHC	19.22	26239621
597	Glutathionylation	GNKFDATCKPLASTT CCCCCCCCCCCCCCC	4.59	24333276
812	Phosphorylation	LKYPQEKTNGPKVPV EECCCHHCCCCCCCE	44.66	18779572
845	Phosphorylation	SFAEGTFTVFAEMYE HHCCCCEEEEEEEEC	17.98	29109428
863	Phosphorylation	LVFGKWGTSGLVGRH EEEECCCCCCCCCCC	20.64	29109428
871	Acetylation	SGLVGRHKFSDVTGK CCCCCCCCCCCCCCC	45.24	-
873	Phosphorylation	LVGRHKFSDVTGKIK CCCCCCCCCCCCCEE	35.65	23984901
978	Acetylation	INRAVDEKGWEYGIT CCHHHCCCCCCCCEE	65.93	23806337
1023	Phosphorylation	KDLTQTASSTARAME HHHHHHHHHHHHHHH	31.44	26824392
1042	Phosphorylation	REGWEYASLIGWKFH CCCHHHHHHCCCEEE	21.44	25338131
1054	Phosphorylation	KFHWKQRSSDTFRRR EEECCCCCCHHHHHH	30.03	29514104
1055	Phosphorylation	FHWKQRSSDTFRRRR EECCCCCCHHHHHHH	42.74	29514104
1154	Phosphorylation	SDPYAHVSFLHRSKT CCCCCCCEEECCCCC	16.54	28973931
1227	Phosphorylation	SPLVKLNSETDITPK CCHHCCCCCCCCCHH	52.92	25338131
1306	Phosphorylation	GLRNMKNYQMASVTS HHHCCCCCEECCCCC	8.31	29899451
1352	Acetylation	FMKVFLPKEELYMPP EEEEECCHHHHCCCC	67.00	23236377
1379	Glutathionylation	RKPVVGQCTIDHLDR CCCCCCEEEECCHHH	2.72	24333276
1380	Phosphorylation	KPVVGQCTIDHLDRF CCCCCEEEECCHHHC	22.88	-
1412	Glutathionylation	SLMSAPPCREVVIEI HHHCCCCCEEEEEEE	5.88	24333276
1433	Acetylation	LASKLSEKEEEIVDW HHHHCCCCHHHHHHH	68.03	23806337
1470	Glutathionylation	SKLKIYDCELEDVAD CCCEEEECCCCCCCC	3.45	24333276
1494	Acetylation	TFKLYRGKSDENEDP HHCHHCCCCCCCCCC	45.82	23806337
1495	Phosphorylation	FKLYRGKSDENEDPS HCHHCCCCCCCCCCC	53.64	30635358
1561	Glutathionylation	PQDNNGLCDPYIKIT CCCCCCCCCCCEEEE	5.40	24333276
1768	Phosphorylation	PGPPFNITPRKAKKY CCCCCCCCHHHCCCE	20.39	27180971
1793	Phosphorylation	DVILDEKSITGEDMS CEEECCCCCCCCCHH	24.28	25338131
1795	Phosphorylation	ILDEKSITGEDMSDI EECCCCCCCCCHHHH	41.43	25338131
2000	S-palmitoylation	FLWFTNPCKTMRFIV EEEECCCCCCHHHHH	6.59	28680068

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MYOF_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MYOF_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MYOF_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of MYOF_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry.";
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
Mol. Cell. Proteomics 8:904-912(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-174, AND MASSSPECTROMETRY.
PubMed: 19131326