RASH_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RASH_MOUSE
• UniProt AC: Q61411
• Protein Name: GTPase HRas
• Gene Name: Hras
• Organism: Mus musculus (Mouse).
• Sequence Length: 189
• Subcellular Localization: Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. Shuttles between the plasma membrane and the Golgi apparatus. The active GTP-bound form is localized most strongly to membranes tha
• Protein Description: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity..
• Protein Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MTEYKLVV -------CCCEEEEE	6.07	-
2	Acetylation	------MTEYKLVVV ------CCCEEEEEE	48.28	-
32	Phosphorylation	QNHFVDEYDPTIEDS HHHCCCCCCCCCCHH	23.82	27717184
39	Phosphorylation	YDPTIEDSYRKQVVI CCCCCCHHHCCEEEE	17.66	23684622
40	Phosphorylation	DPTIEDSYRKQVVID CCCCCHHHCCEEEEC	33.86	27717184
87	Phosphorylation	CVFAINNTKSFEDIH EEEEECCCCCHHHHH	24.47	29899451
101	Ubiquitination	HQYREQIKRVKDSDD HHHHHHHHCCCCCCC	51.32	-
101	Ubiquitination	HQYREQIKRVKDSDD HHHHHHHHCCCCCCC	51.32	22790023
118	S-nitrosocysteine	MVLVGNKCDLAARTV EEEECCHHHHHHHHH	6.34	-
118	S-nitrosylation	MVLVGNKCDLAARTV EEEECCHHHHHHHHH	6.34	-
147	Ubiquitination	PYIETSAKTRQGVED CEEECCCCHHHCHHH	43.96	27667366
147	Ubiquitination	PYIETSAKTRQGVED CEEECCCCHHHCHHH	43.96	22790023
170	Ubiquitination	IRQHKLRKLNPPDES HHHHHHHHCCCCCCC	64.15	-
170	Ubiquitination	IRQHKLRKLNPPDES HHHHHHHHCCCCCCC	64.15	22790023
177	Phosphorylation	KLNPPDESGPGCMSC HCCCCCCCCCCCCCC	58.63	19060867
181	S-palmitoylation	PDESGPGCMSCKCVL CCCCCCCCCCCEEEE	1.72	28680068
184	Other	SGPGCMSCKCVLS-- CCCCCCCCEEEEC--	1.25	-
184	S-palmitoylation	SGPGCMSCKCVLS-- CCCCCCCCEEEEC--	1.25	28680068
186	Methylation	PGCMSCKCVLS---- CCCCCCEEEEC----	4.34	-
186	Farnesylation	PGCMSCKCVLS---- CCCCCCEEEEC----	4.34	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
177	S	Phosphorylation	Kinase	PRKACA	P00517	GPS
177	S	Phosphorylation	Kinase	PRKCA	P05696	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
104	K	Acetylation	Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).	-
170	K	ubiquitylation	Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RASH_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RAF1_HUMAN	RAF1	physical	9261098
RIN1_MOUSE	Rin1	physical	9144171
RAF1_MOUSE	Raf1	physical	9144171
GNDS_MOUSE	Ralgds	physical	9144171
ITF2_MOUSE	Tcf4	genetic	16478791
BRAP_MOUSE	Brap	physical	14724641
RGL3_MOUSE	Rgl3	physical	10869344
VKIND_MOUSE	Kndc1	physical	17984326
CH60_MOUSE	Hspd1	physical	1347942

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.