BRAP_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BRAP_MOUSE
• UniProt AC: Q99MP8
• Protein Name: BRCA1-associated protein
• Gene Name: Brap {ECO:0000312|MGI:MGI:1919649}
• Organism: Mus musculus (Mouse).
• Sequence Length: 591
• Subcellular Localization: Cytoplasm .
• Protein Description: Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport (By similarity)..
• Protein Sequence: MSVSLVVIRLELAGHSPVPTDFGFSAAAGEMSDEEIKKKTLASAVACLEGKSAGEKAAIIHQHLGRREMTDVIIETMKARADEVRDTVEEKKPSAAPVSAQRSREQSESVNTAPESPSKQLPDQISFFSGNPSVEIVHGIMHLYKTNKMTSLKEDVRRSAMLCVLTVPATMTSHDLMKFVAPFNDVIEQMKIIRDSTPNQYMVLIKFSAQADADSFYMACNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDSLELRLSDLLKEKQSVERKCTQLNTRVAKLSTELQEEQELNKCLRANQLVLQNQLKEEEKLLKETCAQKDLQITEIQEQLRDVMFYLETQQQISHLPAETRQEIQEGQINIAMASAPNPPSSGAGGKLQSRKGRSKRGK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
32	Phosphorylation	SAAAGEMSDEEIKKK CCCCCCCCHHHHHHH	37.25	27681418
52	Phosphorylation	VACLEGKSAGEKAAI HHHHCCCCHHHHHHH	52.98	-
78	Ubiquitination	DVIIETMKARADEVR HHHHHHHHHHHHHHH	41.98	22790023
94	Phosphorylation	TVEEKKPSAAPVSAQ HHHHHCCCCCCCCHH	46.04	23375375
103	Phosphorylation	APVSAQRSREQSESV CCCCHHHHHHHHHCC	28.26	25619855
107	Phosphorylation	AQRSREQSESVNTAP HHHHHHHHHCCCCCC	27.38	25619855
109	Phosphorylation	RSREQSESVNTAPES HHHHHHHCCCCCCCC	26.39	25619855
112	Phosphorylation	EQSESVNTAPESPSK HHHHCCCCCCCCCCC	41.24	25619855
116	Phosphorylation	SVNTAPESPSKQLPD CCCCCCCCCCCCCCC	33.15	25521595
118	Phosphorylation	NTAPESPSKQLPDQI CCCCCCCCCCCCCCC	42.17	27742792
232	Ubiquitination	FNSIEDDVCQLVYVE CCCCCCCCEEEEEEE	3.29	27667366
237	Phosphorylation	DDVCQLVYVERAEVL CCCEEEEEEEEHHHE	12.75	25338131
240	Ubiquitination	CQLVYVERAEVLKSE EEEEEEEEHHHEECC	26.46	27667366
304	Phosphorylation	TTCPVCRYCQTPEPV CCCCCCCCCCCCCCC	5.47	20469934
307	Phosphorylation	PVCRYCQTPEPVEEN CCCCCCCCCCCCCCC	26.51	30635358
350	Ubiquitination	HAYKHFEETQHTYAM HHHHHHHHCCCEEEE	53.47	27667366
380	Ubiquitination	VHRLVASKTDGKIVQ CEEEEEECCCCCEEE	40.68	22790023
384	Ubiquitination	VASKTDGKIVQYECE EEECCCCCEEEEEEE	42.38	22790023
399	Ubiquitination	GDTCQEEKIDALQLE CCCCCHHHCCHHHHH	45.17	-
421	Phosphorylation	QLESQRIYWENKIVR HHHHCCEEEECCEEE	14.62	25367039
441	Ubiquitination	AEEINNMKTKFKETI HHHHHHHHHHHHHHH	51.16	22790023
477	Phosphorylation	RKCTQLNTRVAKLST HHHHHHHHHHHHHHH	34.80	18779572
494	Ubiquitination	QEEQELNKCLRANQL HHHHHHHHHHHHHHH	47.47	22790023
508	Ubiquitination	LVLQNQLKEEEKLLK HHHHHHHHHHHHHHH	54.00	22790023
517	Phosphorylation	EEKLLKETCAQKDLQ HHHHHHHHHHHHCCC	16.14	18779572
579	Ubiquitination	PSSGAGGKLQSRKGR CCCCCCCCCCCCCCC	42.30	22790023
582	Phosphorylation	GAGGKLQSRKGRSKR CCCCCCCCCCCCCCC	46.53	29514104

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of BRAP_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of BRAP_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BRAP_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RAF1_MOUSE	Raf1	physical	18332145
KSR1_MOUSE	Ksr1	physical	18332145
MP2K1_MOUSE	Map2k1	physical	18332145
SYNE2_MOUSE	Syne2	physical	23707952
PHLP1_MOUSE	Phlpp1	physical	25820252
AKAP3_MOUSE	Akap3	physical	25820252

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116, AND MASSSPECTROMETRY.
PubMed: 17242355