PHLP1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PHLP1_MOUSE
• UniProt AC: Q8CHE4
• Protein Name: PH domain leucine-rich repeat-containing protein phosphatase 1
• Gene Name: Phlpp1
• Organism: Mus musculus (Mouse).
• Sequence Length: 1687
• Subcellular Localization: Cytoplasm. Membrane; Peripheral membrane protein. Nucleus.
• Protein Description: Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (By similarity). Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity). Involved in the hippocampus-dependent long-term memory formation. [PubMed: 17382888 Involved in circadian control by regulating the consolidation of circadian periodicity after resetting]
• Protein Sequence: MEPAAAAPAQRLADPTGEDQALAAAAAEGGRCPDPALSAAAPSGGNGGAAREEAPCEAPPGPLPGRAGGTGRRRRRGAPQPAAGGAAPVPAAGGGANSLLLKRGRLKRNLSAAAAASSSSSPSSASSAAGGLPASCSASASLCTRSLDRKTLLLKHRQLLQLQPSDRDWVRHQLQRGCVHVFDRHMASSYLRPVLCTLDTTAAEVAARLLQLGHKGGGVVKVLGYGPPPAAAPAASDQTLDGEHGRDVEPPPSSGTVGAVRGPARAPPADLPLPGGAWTRCAPRISPAPSDSSPGELFAGGPGSPPRAPRPASDTESFSLSPSAESVSDRLDPYSSGGGGSSSSSEELEADPAMPHRPGRPAQPRPPSPKTSALLQPKAPTGVDSTGVIAGEGPGDDKAMAAAAPDVPLSTSGRIRETVQKTSPPSLYVQLHGETTRRLEADEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSVSGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQTPTLPAARGLGELQRFTKLKSLNLSNNHLGAFPSAVCSIPTLAELNVSCNALREVPAAVGDMQNLQTFLLDGNFLQSLPAELESMHQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMAGNCVETLRLQALRRMPHIKHVDLRLNILRKLMADEVDFVQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLNVCGYFLKALYASSNELAQLDVYPVPNYLSYMDVSRNCLESVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNRLARLPERLERTSVEVLDVQHNQITELPPNLLMKADSLRFLNASANKLETLPPATLSEETSSILQELYLTNNCLTDKCVPLLTGHPRLKILHMAYNRLQSFPASKMAKLEELEEIDISGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEVKCVDLSCNELSEITLPENLPPKLQELDLTGNPRLALDHKSLELLNNIRCFKIDQPSAGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFRDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKPDPVDLGGSFTLTSANVGKCQTVLCRNGKPLSLSRSYIMSCEEERKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSIDEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGSMPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPSGVLNESSPAYPNEQRCMLHPVCLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFSNGSRVEVEVDIHCSRAKEKERQQHLLQVPAEASDEGIVISANEDESGLSKKADFSAVGTIGRRRANGSVAPQERSHNVIEVAADAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQQQQLPPPPQPPQPQPQPQPQPQPQPQRHFQMDHLPDCYDTPL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEPAAAAP -------CCCCCCCC	53.63	-
98	Phosphorylation	AAGGGANSLLLKRGR CCCCCHHHHHHHCCH	21.62	26824392
102	Ubiquitination	GANSLLLKRGRLKRN CHHHHHHHCCHHHHH	53.42	22790023
111	Phosphorylation	GRLKRNLSAAAAASS CHHHHHHHHHHHHHC	21.29	25293948
117	Phosphorylation	LSAAAAASSSSSPSS HHHHHHHHCCCCCCC	26.48	26643407
118	Phosphorylation	SAAAAASSSSSPSSA HHHHHHHCCCCCCCC	29.52	26643407
126	Phosphorylation	SSSPSSASSAAGGLP CCCCCCCCHHCCCCC	23.58	29899451
146	Phosphorylation	SASLCTRSLDRKTLL HHHHHHCCCCHHHHH	19.23	29514104
151	Phosphorylation	TRSLDRKTLLLKHRQ HCCCCHHHHHHHHHH	24.43	29514104
215	Ubiquitination	RLLQLGHKGGGVVKV HHHHCCCCCCCEEEE	58.41	22790023
221	Acetylation	HKGGGVVKVLGYGPP CCCCCEEEEEECCCC	29.36	30987095
254	Phosphorylation	DVEPPPSSGTVGAVR CCCCCCCCCCCCCCC	44.23	21454597
256	Phosphorylation	EPPPSSGTVGAVRGP CCCCCCCCCCCCCCC	20.08	21454597
286	Phosphorylation	TRCAPRISPAPSDSS CCCCCCCCCCCCCCC	19.11	26824392
290	Phosphorylation	PRISPAPSDSSPGEL CCCCCCCCCCCCCCC	52.83	25293948
292	Phosphorylation	ISPAPSDSSPGELFA CCCCCCCCCCCCCCC	42.65	25293948
293	Phosphorylation	SPAPSDSSPGELFAG CCCCCCCCCCCCCCC	41.75	25293948
304	Phosphorylation	LFAGGPGSPPRAPRP CCCCCCCCCCCCCCC	34.56	22817900
313	Phosphorylation	PRAPRPASDTESFSL CCCCCCCCCCCCCCC	47.99	25338131
368	Phosphorylation	PAQPRPPSPKTSALL CCCCCCCCCCCCHHH	41.53	28066266
372	Phosphorylation	RPPSPKTSALLQPKA CCCCCCCCHHHCCCC	24.05	-
410	Phosphorylation	AAPDVPLSTSGRIRE HCCCCCCCCCCHHHH	17.92	25338131
421	Ubiquitination	RIRETVQKTSPPSLY HHHHHHHCCCCCCEE	46.78	22790023
629	Methylation	NLKQNFLRQTPTLPA CCCHHHHHCCCCCHH	33.90	58858887
638	Methylation	TPTLPAARGLGELQR CCCCHHHCCHHHHHH	42.87	58858893
931	Phosphorylation	DVQHNQITELPPNLL ECCCCCCCCCCCCHH	23.00	-
943	Phosphorylation	NLLMKADSLRFLNAS CHHHHHHHHHHHCCC	26.72	-
1102	Ubiquitination	PRLALDHKSLELLNN CCCCCCHHHHHHHHC	57.30	22790023
1480	Phosphorylation	MLHPVCLSNSFQRQL CCCHHHHCHHHHHHH	25.87	22324799
1482	Phosphorylation	HPVCLSNSFQRQLSS CHHHHCHHHHHHHHH	21.27	22324799
1503	Phosphorylation	FSDNGLDSDDEEPIE HCCCCCCCCCCCCCC	52.59	22817900
1573	Phosphorylation	ADFSAVGTIGRRRAN CCCCCCCCCCCCCCC	17.41	26824392

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PHLP1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PHLP1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PHLP1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BRAP_MOUSE	Brap	physical	25820252

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.