RAP1B_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RAP1B_MOUSE
• UniProt AC: Q99JI6
• Protein Name: Ras-related protein Rap-1b
• Gene Name: Rap1b
• Organism: Mus musculus (Mouse).
• Sequence Length: 184
• Subcellular Localization: Cell membrane. Cytoplasm, cytosol. Cell junction. May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction (By similarity)..
• Protein Description: GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function (By similarity)..
• Protein Sequence: MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Ubiquitination	---MREYKLVVLGSG ---CCEEEEEEECCC	30.01	-
11	Phosphorylation	YKLVVLGSGGVGKSA EEEEEECCCCCCCHH	28.36	28066266
31	Ubiquitination	VQGIFVEKYDPTIED EEEEEEEECCCCCCH	50.04	-
32	Phosphorylation	QGIFVEKYDPTIEDS EEEEEEECCCCCCHH	17.41	29514104
39	ADP-ribosylation	YDPTIEDSYRKQVEV CCCCCCHHHHHHHEE	17.66	-
39	Phosphorylation	YDPTIEDSYRKQVEV CCCCCCHHHHHHHEE	17.66	30635358
40	Phosphorylation	DPTIEDSYRKQVEVD CCCCCHHHHHHHEEC	33.86	27717184
51	Glutathionylation	VEVDAQQCMLEILDT HEECHHHHHHHHHHH	1.92	24333276
106	Phosphorylation	QILRVKDTDDVPMIL HHHCCCCCCCCCEEE	28.51	20469934
117	Ubiquitination	PMILVGNKCDLEDER CEEEECCCCCCCCCE	24.17	-
118	S-nitrosocysteine	MILVGNKCDLEDERV EEEECCCCCCCCCEE	9.29	-
118	S-nitrosylation	MILVGNKCDLEDERV EEEECCCCCCCCCEE	9.29	21278135
118	Glutathionylation	MILVGNKCDLEDERV EEEECCCCCCCCCEE	9.29	24333276
128	Ubiquitination	EDERVVGKEQGQNLA CCCEECCHHHHHHHH	34.94	-
128	Malonylation	EDERVVGKEQGQNLA CCCEECCHHHHHHHH	34.94	26320211
141	S-nitrosylation	LARQWNNCAFLESSA HHHHHHCCHHHHHHC	2.27	24895380
141	S-nitrosocysteine	LARQWNNCAFLESSA HHHHHHCCHHHHHHC	2.27	-
149	Ubiquitination	AFLESSAKSKINVNE HHHHHHCCCCCCHHH	54.98	-
150	Phosphorylation	FLESSAKSKINVNEI HHHHHCCCCCCHHHH	38.18	21082442
151	Ubiquitination	LESSAKSKINVNEIF HHHHCCCCCCHHHHH	37.28	-
159	Phosphorylation	INVNEIFYDLVRQIN CCHHHHHHHHHHHHC	17.65	22817900
174	Malonylation	RKTPVPGKARKKSSC CCCCCCCCCCCCCCC	38.66	26320211
179	Phosphorylation	PGKARKKSSCQLL-- CCCCCCCCCCCCC--	39.11	24759943
180	Phosphorylation	GKARKKSSCQLL--- CCCCCCCCCCCC---	18.11	24759943
181	Methylation	KARKKSSCQLL---- CCCCCCCCCCC----	4.22	-
181	Geranylgeranylation	KARKKSSCQLL---- CCCCCCCCCCC----	4.22	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
179	S	Phosphorylation	Kinase	PKA	-	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Smurf2	A2A5Z6	PMID:22199232

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RAP1B_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RAP1B_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of RAP1B_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.