TRAF6_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TRAF6_MOUSE
• UniProt AC: P70196
• Protein Name: TNF receptor-associated factor 6
• Gene Name: Traf6
• Organism: Mus musculus (Mouse).
• Sequence Length: 530
• Subcellular Localization: Cytoplasm . Cytoplasm, cell cortex . Nucleus . Lipid droplet . RSAD2/viperin recruits it to the lipid droplet.
• Protein Description: E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation (By similarity). Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production..
• Protein Sequence: MSLLNCENSCGSSQSSSDCCAAMAASCSAAVKDDSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYDLDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSRGCRPEDPNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDKVAEMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIKDDTLLVRCEVSTRFDMGGLRKEGFQPRSTDAGV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	SLLNCENSCGSSQSS CCCCCCCCCCCCCCH	9.50	-
12	Phosphorylation	NCENSCGSSQSSSDC CCCCCCCCCCCHHHH	29.54	-
13	Phosphorylation	CENSCGSSQSSSDCC CCCCCCCCCCHHHHH	21.26	-
15	Phosphorylation	NSCGSSQSSSDCCAA CCCCCCCCHHHHHHH	33.48	-
46	Phosphorylation	SASTGNLSSSFMEEI CCCCCCCCHHHHHHH	28.12	-
47	Phosphorylation	ASTGNLSSSFMEEIQ CCCCCCCHHHHHHHC	30.66	-
48	Phosphorylation	STGNLSSSFMEEIQG CCCCCCHHHHHHHCC	25.82	-
195	Phosphorylation	SCVNCAVSMAYEEKE CHHHHHHHHHHHCHH	4.52	29514104
291	Phosphorylation	LRPCDAASPSRGCRP ECCCCCCCCCCCCCC	25.73	25266776
293	Phosphorylation	PCDAASPSRGCRPED CCCCCCCCCCCCCCC	38.52	23984901
330	Phosphorylation	ELTAKMETQSMYVGE HHHHHHHHCHHHHHH	23.57	28576409
334	Phosphorylation	KMETQSMYVGELKRT HHHHCHHHHHHHHHH	15.56	28576409
471	Phosphorylation	LLAFQRPTIPRNPKG HHHEECCCCCCCCCC	45.89	-
494	Phosphorylation	LEALRQGTFIKDDTL HHHHHCCCCCCCCEE	17.53	-
525	Phosphorylation	KEGFQPRSTDAGV-- CCCCCCCCCCCCC--	37.00	30635358
526	Phosphorylation	EGFQPRSTDAGV--- CCCCCCCCCCCC---	31.02	30635358

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
46	S	Phosphorylation	Kinase	RPS6KA3	P18654	GPS
47	S	Phosphorylation	Kinase	RPS6KA3	P18654	GPS
48	S	Phosphorylation	Kinase	RPS6KA3	P18654	GPS
471	T	Phosphorylation	Kinase	MST4	Q9P289	PSP
471	T	Phosphorylation	Kinase	MST4	Q99JT2	PSP
494	T	Phosphorylation	Kinase	MST4	Q9P289	PSP
494	T	Phosphorylation	Kinase	MST4	Q99JT2	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	Traf6	P70196	PMID:22199232
-	K	Ubiquitination	E3 ubiquitin ligase	Trim38	Q5SZ99	PMID:22323536

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
63	K	ubiquitylation	LRRC19 induces 'Lys-63' ubiquitination.	25026888
63	K	ubiquitylation	This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules.	25026888
124	K	Sumoylation	Sumoylated on Lys-124, Lys-142 and Lys-461 with SUMO1.	-
124	K	ubiquitylation	Polyubiquitinated on Lys-124 by TRAF3IP2; after cell stimulation with IL17A (By similarity).	25026888
142	K	Sumoylation	Sumoylated on Lys-124, Lys-142 and Lys-461 with SUMO1.	-
461	K	Sumoylation	Sumoylated on Lys-124, Lys-142 and Lys-461 with SUMO1.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TRAF6_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TIFA_MOUSE	Tifa	physical	12566447
ECSIT_MOUSE	Ecsit	physical	10465784
CYLD_MOUSE	Cyld	physical	18382763
CYLD_MOUSE	Cyld	physical	18174161
MALT1_MOUSE	Malt1	physical	16831874
IRAK1_MOUSE	Irak1	physical	16831874
IRAK3_MOUSE	Irak3	physical	21098228
CITE2_MOUSE	Cited2	genetic	21098220
ITP_HHV11	UL37	physical	18682563
ACTN4_MOUSE	Actn4	physical	16196101
ACTN1_MOUSE	Actn1	physical	16196101
WDR24_MOUSE	Wdr24	physical	16196101
EF2_MOUSE	Eef2	physical	16196101
HS90B_MOUSE	Hsp90ab1	physical	16196101
HS90A_MOUSE	Hsp90aa1	physical	16196101
GRP78_MOUSE	Hspa5	physical	16196101
GRP75_MOUSE	Hspa9	physical	16196101
VATA_MOUSE	Atp6v1a	physical	16196101
HSP7C_MOUSE	Hspa8	physical	16196101
HSP72_MOUSE	Hspa2	physical	16196101
KPYM_MOUSE	Pkm	physical	16196101
ATPB_MOUSE	Atp5b	physical	16196101
EF1A1_MOUSE	Eef1a1	physical	16196101
ARP3_MOUSE	Actr3	physical	16196101
ENOA_MOUSE	Eno1	physical	16196101
ACTB_MOUSE	Actb	physical	16196101
ALDOA_MOUSE	Aldoa	physical	16196101
NEMO_MOUSE	Ikbkg	physical	21566132
NEMO_MOUSE	Ikbkg	physical	19136968
SRA1_MOUSE	Sra1	physical	21460221
UBE2N_MOUSE	Ube2n	physical	20185725
TNAP3_MOUSE	Tnfaip3	physical	20185725
UB2D3_MOUSE	Ube2d3	physical	20185725
ITCH_MOUSE	Itch	physical	20185725
RNF11_MOUSE	Rnf11	physical	20185725
TAXB1_MOUSE	Tax1bp1	physical	20185725
NLRX1_MOUSE	Nlrx1	physical	21703539
TAXB1_MOUSE	Tax1bp1	physical	21765415
ITCH_MOUSE	Itch	physical	21765415
RNF11_MOUSE	Rnf11	physical	21765415
UBE2N_MOUSE	Ube2n	physical	21765415
UB2D3_MOUSE	Ube2d3	physical	21765415
TNAP3_MOUSE	Tnfaip3	physical	21765415
UBE2N_HUMAN	UBE2N	physical	15147900
TRAF6_MOUSE	Traf6	physical	15147900
ITCH_MOUSE	Itch	physical	18246070
ARRB1_MOUSE	Arrb1	physical	16378096
ARRB2_MOUSE	Arrb2	physical	16378096
IRF5_MOUSE	Irf5	physical	18824541
FHL2_MOUSE	Fhl2	physical	16184196
NR0B2_MOUSE	Nr0b2	physical	14500659
TRFL_BOVIN	LTF	physical	22593578
TAXB1_MOUSE	Tax1bp1	physical	18239685
IRF7_MOUSE	Irf7	physical	21743479
M3K7_MOUSE	Map3k7	physical	22649194
ITCH_MOUSE	Itch	physical	23782702
CYLD_MOUSE	Cyld	physical	23782702
WWP1_MOUSE	Wwp1	physical	23799152
MAVS_MOUSE	Mavs	physical	23951545
AKT1_MOUSE	Akt1	physical	23907468
PR15A_MOUSE	Ppp1r15a	physical	23907468
MTOR_MOUSE	Mtor	physical	23911927
KSYK_MOUSE	Syk	physical	23962979
TLR4_MOUSE	Tlr4	physical	23962979
ARRB2_MOUSE	Arrb2	physical	23960234
M3K7_MOUSE	Map3k7	physical	23960234
TRAD1_MOUSE	Trafd1	physical	16221674
CHIP_MOUSE	Stub1	physical	24578159
PKHO1_MOUSE	Plekho1	physical	24777252
HS71B_MOUSE	Hspa1b	physical	16697380
UBE2N_HUMAN	UBE2N	physical	17709375
UB2V1_HUMAN	UBE2V1	physical	17709375
TRAF6_MOUSE	Traf6	physical	17709375
UB2D3_HUMAN	UBE2D3	physical	17709375
UBE2N_MOUSE	Ube2n	physical	19136968
AKT1_MOUSE	Akt1	physical	25808491
STK26_MOUSE	Stk26	physical	25642822
MYSM1_MOUSE	Mysm1	physical	26474655
TRAF3_MOUSE	Traf3	physical	26474655
TAB2_MOUSE	Tab2	physical	26474655
TAB3_MOUSE	Tab3	physical	26474655
NEMO_MOUSE	Ikbkg	physical	26474655
M3K7_MOUSE	Map3k7	physical	26474655
GSK3B_MOUSE	Gsk3b	physical	25828701
M3K7_MOUSE	Map3k7	physical	25828701
TAB1_MOUSE	Tab1	physical	25828701
TAB2_MOUSE	Tab2	physical	25828701
TCAM2_MOUSE	Ticam2	physical	25828701
SMN_MOUSE	Smn1	physical	28214532
VAV3_MOUSE	Vav3	physical	27507811
CUL5_MOUSE	Cul5	physical	27233966

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.