IRF7_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: IRF7_MOUSE
• UniProt AC: P70434
• Protein Name: Interferon regulatory factor 7
• Gene Name: Irf7
• Organism: Mus musculus (Mouse).
• Sequence Length: 457
• Subcellular Localization: Nucleus . Cytoplasm . The phosphorylated and active form accumulates selectively in the nucleus.
• Protein Description: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages..
• Protein Sequence: MAEVRGVQRVLFGDWLLGEVSSGQYEGLQWLNEARTVFRVPWKHFGRRDLDEEDAQIFKAWAVARGRWPPSGVNLPPPEAEAAERRERRGWKTNFRCALHSTGRFILRQDNSGDPVDPHKVYELSRELGSTVGPATENREEVSLSNALPTQGVSPGSFLARENAGLQTPSPLLSSDAGDLLLQVLQYSHILESESGADPVPPQAPGQEQDRVYEEPYAAWQVEAVPSPRPQQPALTERSLGFLDVTIMYKGRTVLQAVVGHPRCVFLYSPMAPAVRTSEPQPVIFPSPAELPDQKQLHYTETLLQHVSPGLQLELRGPSLWALRMGKCKVYWEVGSPMGTTGPSTPPQLLERNRHTPIFDFSTFFRELEEFRARRRQGSPHYTIYLGFGQDLSAGRPKEKTLILVKLEPWVCKAYLEGVQREGVSSLDSSSLGLCLSSTNSLYEDIEHFLMDLGQWP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
59	Ubiquitination	EEDAQIFKAWAVARG HHHHHHHHHHHHHCC	44.65	22790023
92	Acetylation	RRERRGWKTNFRCAL HHHHHCCCCCCHHHC	36.09	-
406	Sumoylation	EKTLILVKLEPWVCK CCEEEEEECCHHHHH	43.62	-
406	Sumoylation	EKTLILVKLEPWVCK CCEEEEEECCHHHHH	43.62	-
413	Ubiquitination	KLEPWVCKAYLEGVQ ECCHHHHHHHHHHHH	30.37	22790023
425	Phosphorylation	GVQREGVSSLDSSSL HHHHCCCCCCCCCCH	35.72	15743772
426	Phosphorylation	VQREGVSSLDSSSLG HHHCCCCCCCCCCHH	33.51	15743772
429	Phosphorylation	EGVSSLDSSSLGLCL CCCCCCCCCCHHHHH	27.41	15743772
431	Phosphorylation	VSSLDSSSLGLCLSS CCCCCCCCHHHHHHC	30.34	22817900
437	Phosphorylation	SSLGLCLSSTNSLYE CCHHHHHHCCCHHHH	33.83	15743772
438	Phosphorylation	SLGLCLSSTNSLYED CHHHHHHCCCHHHHH	20.75	15743772
441	Phosphorylation	LCLSSTNSLYEDIEH HHHHCCCHHHHHHHH	32.20	15743772

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
431	S	Phosphorylation	Kinase	IKKE	Q9R0T8	Uniprot
431	S	Phosphorylation	Kinase	TBK1	Q9WUN2	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of IRF7_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of IRF7_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TRAF6_MOUSE	Traf6	physical	23042151
TRI35_MOUSE	Trim35	physical	25907537

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.